{"id":108150,"date":"2017-11-01T06:30:06","date_gmt":"2017-10-31T22:30:06","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=108150"},"modified":"2017-11-01T09:04:09","modified_gmt":"2017-11-01T01:04:09","slug":"%e8%a7%82%e7%82%b9%e4%b8%a8%e6%b5%81%e5%bc%8f%e7%bb%86%e8%83%9e%e4%bb%aa%e7%9a%84%e5%8e%9f%e7%90%86%e3%80%81%e5%ba%94%e7%94%a8%e5%8f%8a%e6%9c%80%e6%96%b0%e8%bf%9b%e5%b1%95","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=108150","title":{"rendered":"\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u539f\u7406\u3001\u5e94\u7528\u53ca\u6700\u65b0\u8fdb\u5c55"},"content":{"rendered":"

\u524d\u8a00<\/strong><\/p>\n

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1 \u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u548c\u6280\u672f\u6307\u6807<\/strong><\/span><\/p>\n

1.1 \u6d41\u5f0f\u7ec6\u80de\u4eea\u5de5\u4f5c\u539f\u7406<\/strong><\/p>\n

\u56fe1\u4e3a\u6d41\u5f0f\u7ec6\u80de\u4eea\u57fa\u672c\u7ed3\u6784\u3002\u5b83\u4e3b\u8981\u6709\u56db\u90e8\u5206\u7ec4\u6210\uff1a\u6d41\u52a8\u5ba4\u548c\u6db2\u6d41\u7cfb\u7edf\uff1b\u5149\u6e90\u4e0e\u5149\u5b66\u7cfb\u7edf\u3001\u4fe1\u53f7\u6536\u96c6\u4e0e\u4fe1\u53f7\u8f6c\u6362\u7cfb\u7edf\u3001\u8ba1\u7b97\u673a\u4e0e\u5206\u6790\u7cfb\u7edf\u3002\u5177\u6709\u5206\u9009\u529f\u80fd\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fd8\u5305\u62ec\u5206\u9009\u7cfb\u7edf\u3002\u5c06\u5f85\u6d4b\u7ec6\u80de\u8367\u5149\u67d3\u8272\u540e\u5236\u6210\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u5728\u9798\u6db2\u7684\u5305\u88ab\u4e0b\u5355\u884c\u6392\u5217\uff0c\u4f9d\u6b21\u901a\u8fc7\u6d41\u52a8\u5ba4\u68c0\u6d4b\u533a\u57df\u3002\u4ee5\u6fc0\u5149\u4f5c\u4e3a\u6fc0\u53d1\u5149\u6e90\uff0c\u5782\u76f4\u7167\u5c04\u68c0\u6d4b\u533a\u57df\u7684\u6837\u54c1\u6d41\uff0c\u88ab\u8367\u5149\u67d3\u8272\u7684\u7ec6\u80de\u5728\u5176\u7167\u5c04\u4e0b\uff0c\u4ea7\u751f\u6563\u5c04\u5149\u548c\u6fc0\u53d1\u8367\u5149\uff0c\u5b83\u4eec\u540c\u65f6\u88ab\u524d\u5411\u5149\u7535\u4e8c\u6781\u7ba1\u548c\u4fa7\u541190\u00b0\u65b9\u5411\u7684\u5149\u7535\u500d\u589e\u7ba1\u63a5\u6536\u3002\u524d\u5411\u5c0f\u89d2\u5ea6\u7684\u5149\u6563\u5c04\u4fe1\u53f7\uff08forward scatter,FSC\uff09\u53cd\u6620\u4e86\u7ec6\u80de\u4f53\u79ef\u7684\u5927\u5c0f\uff1b\u4fa7\u541190\u00b0\u65b9\u5411\u7684\u5149\u6563\u5c04\u4fe1\u53f7\uff08side scatter\uff0cSSC\uff09\u53cd\u6620\u4e86\u7ec6\u80de\u5185\u9897\u7c92\u7684\u590d\u6742\u60c5\u51b5\u3002\u6fc0\u53d1\u8367\u5149\u4fe1\u53f7\u4ee3\u8868\u4e86\u6240\u6807\u8bb0\u7684\u88ab\u6d4b\u7ec6\u80de\u5185\u90e8\u9897\u7c92\u7684\u4fe1\u606f\u3002\u8fd9\u4e9b\u5149\u4fe1\u53f7\u8f6c\u5316\u6210\u7535\u4fe1\u53f7\uff0c\u88ab\u4f20\u9001\u5230\u8ba1\u7b97\u673a\uff0c\u7ecfA\/D\u8f6c\u6362\u5668\u4f20\u8f93\u5230\u5fae\u673a\u5904\u7406\u5668\u5f62\u6210\u6570\u636e\u6587\u4ef6\uff0c\u4fdd\u5b58\u5230\u8ba1\u7b97\u673a\u4e0a\uff0c\u4ee5\u5907\u8131\u673a\u540e\u7684\u6570\u636e\u5904\u7406\u548c\u5206\u6790\u3002\u7ec6\u80de\u7684\u5206\u9009\u662f\u5c06\u5f85\u6d4b\u6db2\u6ef4\u5145\u4ee5\u6b63\u8d1f\u4e0d\u540c\u7684\u7535\u8377\uff0c\u8ba9\u5176\u5728\u9ad8\u538b\u7535\u573a\u7684\u4f5c\u7528\u4e0b\u504f\u8f6c\uff0c\u843d\u5165\u4e0d\u540c\u7684\u6536\u96c6\u5bb9\u5668\u4e2d\uff0c\u4ece\u800c\u5b9e\u73b0\u7ec6\u80de\u7684\u5206\u79bb\u3002<\/p>\n

\"\"<\/p>\n

\u56fe 1 \u6d41\u5f0f\u7ec6\u80de\u4eea\u7ed3\u6784\u793a\u610f\u56fe<\/p>\n

1.2 \u6d41\u5f0f\u7ec6\u80de\u4eea\u6027\u80fd\u7684\u6280\u672f\u6307\u6807<\/strong><\/p>\n

\u6d41\u5f0f\u7ec6\u80de\u4eea\u6027\u80fd\u7684\u6280\u672f\u6307\u6807\u4e3b\u8981\u6709\u8367\u5149\u5206\u8fa8\u7387\u3001\u8367\u5149\u7075\u654f\u5ea6\u3001\u5206\u6790\u53c2\u6570\u591a\u5c11\u53ca\u5206\u6790\u3001\u5206\u9009\u901f\u5ea6\u53ca\u7eaf\u5ea6\u7b49\u3002\u8367\u5149\u5206\u8fa8\u7387\u662f\u6307\u5206\u8fa8\u4e24\u4e2a\u76f8\u90bb\u5cf0\u7684\u6700\u5c0f\u8ddd\u79bb\uff0c\u901a\u5e38\u7528\u53d8\u5f02\u7cfb\u6570\uff08CV\u503c\uff09\u6765\u8868\u793a\u3002\u73b0\u5728\u5e02\u573a\u4e0a\u4e3b\u6d41\u578b\u53f7\u7684\u8367\u5149\u5206\u8fa8\u7387\u5c0f\u4e8e2.0%\uff0c\u6709\u7684\u751a\u81f3\u57281%\u4e4b\u5185\u3002\u8367\u5149\u7075\u654f\u5ea6\u53cd\u6620\u4e86\u4eea\u5668\u63a2\u6d4b\u6700\u5c0f\u8367\u5149\u5149\u5f3a\u7684\u80fd\u529b\uff0c\u4e00\u822c\u7528\u8367\u5149\u5fae\u7403\u4e0a\u53ef\u6d4b\u51fa\u7684\u8367\u5149\uff08FITC\uff09\u7684\u6700\u5c0f\u5206\u5b50\u6570\u6765\u8868\u793a\u3002\u76ee\u524d\u4eea\u5668\u53ef\u4ee5\u8fbe\u5230100\u4ee5\u5185\u3002\u4e00\u822c\u5206\u6790\u901f\u5ea6\u4e3a500-10000\u4e2a\u7ec6\u80de\/s\uff0c\u5206\u9009\u901f\u5ea6\u63a7\u5236\u57281000\u4e2a\u7ec6\u80de\/s\u4ee5\u4e0b\uff0c\u5206\u9009\u7eaf\u5ea6>98%,\u6709\u7684\u4eea\u5668\uff08\u5982 MoFLo High-Performance Cell Sorter\uff09\u5206\u6790\u901f\u5ea6\u53ef\u8fbe100000\u4e2a\u7ec6\u80de\/s\uff0c\u5206\u9009\u901f\u5ea6\u53ef\u8fbe70000\u4e2a\u7ec6\u80de\/s\uff0c\u5206\u9009\u7eaf\u5ea6>98%\u3002\u5e94\u7528\u65b9\u5411\u4e0d\u540c\uff0c\u5206\u6790\u53c2\u6570\u7684\u591a\u5c11\u5dee\u5f02\u8f83\u5927\uff0c\u5c11\u7684\uff08\u5982Guava PCA-96\uff09\u53ef\u4ee5\u5206\u67903\u4e2a\u8367\u5149\u53c2\u6570\u548c1\u4e2a\u6563\u5c04\u4fe1\u53f7\uff0c\u591a\u7684\uff08\u5982CyFlow SL\uff09\u53ef\u4ee5\u5206\u679013\u4e2a\u8367\u5149\u53c2\u6570\u548c3\u4e2a\u6563\u5c04\u4fe1\u53f7\u3002<\/p>\n

2 \u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u5e94\u7528<\/strong><\/span><\/p>\n

2.1 \u5728\u4e34\u5e8a\u4e0a\u7684\u5e94\u7528<\/strong><\/p>\n

\u7ec6\u80de\u764c\u53d8\u8fc7\u7a0b\u4e2d\u4f34\u968f\u7ec6\u80deDNA\u542b\u91cf\u7684\u6539\u53d8\uff0cDNA\u975e\u6574\u500d\u4f53\u7ec6\u80de\u662f\u6076\u6027\u80bf\u7624\u7684\u7279\u5f02\u6027\u6807\u5fd7\u3002\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684FSC\u80fd\u591f\u7ed9\u51fa\u8fd9\u4e9b\u4fe1\u606f\uff0c\u5728\u80bf\u7624\u7684\u65e9\u671f\u8bca\u65ad\u548c\u9274\u522b\u8bca\u65ad\u4e2d\u6709\u7740\u5f88\u91cd\u8981\u7684\u4f5c\u7528\uff1b\u65e9\u572880\u5e74\u4ee3\u4eba\u4eec\u53d1\u73b0\u7279\u5b9aT\u7ec6\u80de\u4e9a\u7fa4\u53ef\u4ee5\u53cd\u6620AIDS\u60a3\u8005\u7684\u4e34\u5e8a\u72b6\u6001\uff0c\u800c\u6d41\u5f0f\u7ec6\u80de\u4eea\u53ef\u4ee5\u5bf9\u8fd9\u4e9bT\u7ec6\u80de\u4e9a\u7fa4\u8fdb\u884c\u68c0\u6d4b\uff0c\u4e3aAIDS\u7684\u8bca\u65ad\u3001\u6cbb\u7597\u63d0\u4f9b\u6709\u7528\u4fe1\u606f\uff1b\u6d41\u5f0f\u7ec6\u80de\u4eea\u501f\u52a9\u4e8e\u5404\u79cd\u8367\u5149\u67d3\u6599\u6d4b\u5b9a\u4e00\u4e2a\u7ec6\u80de\u7684\u591a\u79cd\u53c2\u6570\u5b8c\u6210\u767d\u8840\u75c5\u53ca\u6dcb\u5df4\u7624\u7684\u8bca\u65ad\uff0c\u51c6\u786e\u7387\u8fbe90%\u5de6\u53f3\uff0c\u8f83\u4f20\u7edf\u5f62\u6001\u5b66\u68c0\u67e5\u51c6\u786e\u6027\u63d0\u9ad810%-20%\uff1b\u6d41\u5f0f\u7ec6\u80de\u4eea\u5bf9\u6dcb\u5df4\u7ec6\u80de\u53ca\u5176\u4e9a\u7fa4\u6570\u91cf\u7684\u68c0\u6d4b\uff0c\u53ef\u4ee5\u63a2\u7d22\u75be\u75c5\u7684\u53d1\u75c5\u673a\u7406\u3001\u75c5\u7a0b\u3001\u9884\u540e\uff0c\u6307\u5bfc\u4e34\u5e8a\u6cbb\u7597\u65b9\u6848\u3002<\/p>\n

2.2 \u5728\u751f\u7269\u5b66\u53ca\u5fae\u751f\u7269\u5b66\u4e0a\u7684\u5e94\u7528<\/strong><\/p>\n

\u4ece\u65e9\u671f\u5230\u73b0\u5728\uff0c\u5728\u7ec6\u80de\u53ca\u7ec6\u80de\u5668\u65b9\u9762\u5f88\u591a\u5df2\u88ab\u4eba\u4eec\u6240\u63a5\u53d7\u7684\u79d1\u6280\uff0c\u90fd\u7ecf\u6d41\u5f0f\u7ec6\u80de\u4eea\u7814\u7a76\u8fc7\u3002\u5728\u7ec6\u80de\u529f\u80fd\u7684\u65e9\u671f\u7814\u7a76\u4e2d\uff0c\u7814\u7a76\u8005\u4ec5\u80fd\u901a\u8fc7\u89c2\u6d4b\u5fae\u751f\u7269\u7684\u541e\u566c\u6765\u7814\u7a76\u566c\u4e2d\u6027\u7c92\u5b50,\u800c\u6d41\u5f0f\u7ec6\u80de\u4eea\u53ef\u4ee5\u901a\u8fc7\u5b83\u4eec\u7684\u5927\u5c0f\u3001\u5f62\u72b6\u3001\u6297\u4f53\u53ca\u541e\u566c\u8fc7\u7a0b\u4e2d\u5fae\u751f\u7269\u79cd\u7c7b\u3001\u6570\u91cf\u7684\u53d8\u5316\u66f4\u6df1\u5165\u7684\u8fa8\u522b\u3001\u7814\u7a76\u8fd9\u4e9b\u566c\u4e2d\u6027\u7c92\u5b50\uff1b\u6c27\u81ea\u7531\u57fa\u7684\u5b9e\u65f6\u3001\u5355\u7ec6\u80de\u7e41\u6b96\u8bc4\u4ef7\uff0cDNA\u500d\u4f53\u5206\u6790\uff0c\u7ec6\u80de\u5faa\u73af\u4e2d\u7ec6\u80de\u589e\u6b96\u548c\u51cb\u4ea1\u5206\u6790\u7b49\u90fd\u4f1a\u7528\u5230\u6d41\u5f0f\u7ec6\u80de\u4eea\uff1b\u6d41\u5f0f\u7ec6\u80de\u4eea\u5bf9\u7ec6\u80de\u5468\u671f\u53ef\u4ee5\u8f7b\u677e\u5206\u6790\uff0c\u4fc3\u8fdb\u4e86\u4ee5\u6b64\u4e3a\u57fa\u7840\u7684\u690d\u7269\u5b66\u7684\u53d1\u5c55\uff0c\u8f85\u4ee5\u6210\u50cf\u4eea\u5668\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u53ef\u4ee5\u5bf9\u5fae\u751f\u7269\u7684\u6210\u957f\u3001\u7e41\u6b96\u8fdb\u884c3-D\u7814\u7a76\u3002<\/p>\n

2.3 \u5728\u751f\u6b96\u5b66\u53ca\u5236\u836f\u5b66\u4e0a\u7684\u5e94\u7528<\/strong><\/p>\n

\u5229\u7528\u5bf9\u7cbe\u5b50DNA\u8fdb\u884c\u65e0\u5bb3\u6807\u5b9a\u7684DNA\u67d3\u6599(\u5982Hoechst 33342)\u6216H-Y\u6297\u4f53\u90fd\u53ef\u4ee5\u5bf9\u7cbe\u5b50\u8fdb\u884c\u68c0\u6d4b\u548c\u5206\u7c7b\uff0c\u5b8c\u6210\u751f\u6b96\u5b66\u4e0a\u7684\u8981\u6c42\uff1bFCM\u68c0\u6d4b\u80bf\u7624\u7ec6\u80de\u7684\u8010\u836f\u76f8\u5173\u86cb\u767d\u7684\u8868\u8fbe\u6c34\u5e73\uff0c\u5bf9\u68c0\u6d4b\u4e34\u5e8a\u80bf\u7624\u5316\u7597\u6548\u679c\u53ca\u836f\u7269\u7684\u9009\u62e9\u6709\u4e00\u5b9a\u7684\u610f\u4e49\u3002\u6b64\u5916\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u5728\u7ec6\u80de\u6bd2\uff0c\u53ef\u6eb6\u6027\u86cb\u767d\u8d28\u5206\u5b50\uff0c\u9499\u79bb\u5b50\u6d53\u5ea6\u68c0\u6d4b\u7b49\u90fd\u6709\u5f88\u5e7f\u6cdb\u7684\u7528\u9014\u3002\u6b64\u5916\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u5728\u7ec6\u80de\u6bd2\uff0c\u53ef\u6eb6\u6027\u86cb\u767d\u8d28\u5206\u5b50\uff0c\u9499\u79bb\u5b50\u6d53\u5ea6\u68c0\u6d4b\u7b49\u90fd\u6709\u5f88\u5e7f\u6cdb\u7684\u7528\u9014\u3002<\/p>\n

3 \u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u6700\u65b0\u53d1\u5c55<\/span><\/strong><\/p>\n

3.1 \u4eea\u5668\u529f\u80fd\u4e13\u4e1a\u5316<\/strong><\/p>\n

Partec CyFlow\u3001BD FACSCount\u53ef\u4ee5\u5b9e\u73b0CD4,CD8,CD3\u7edd\u5bf9\u8ba1\u6570\uff0c\u7528\u4e8eHIV\u68c0\u6d4b\u7684\u7ecf\u6d4e\u666e\u53ca\u578b\u6d41\u5f0f\u7ec6\u80de\u4eea\u3002<\/p>\n

3.2 \u4eea\u5668\u5168\u9762\u81ea\u52a8\u5316<\/strong><\/p>\n

\u514d\u75ab\u6807\u672c\u5236\u5907\u4eea\u3001\u7ec4\u7ec7\u6837\u672c\u5236\u5907\u4eea\u548c\u6837\u54c1\u524d\u5904\u7406\u4eea\u5728\u5b9e\u9a8c\u4e2d\u5f97\u5230\u5e7f\u6cdb\u5e94\u7528\u3002\u4e0d\u540c\u89c4\u683c\u7684\u591a\u5b54\u677f\u6216\u591a\u8bd5\u7ba1\u81ea\u52a8\u8fdb\u6837\u5668\u52a0\u901f\u4e86\u81ea\u52a8\u5316\u8fdb\u7a0b\u3002Epics XL\u3001FACSCalibur \u7b49\u4e3b\u6d41\u5382\u5bb6\u4eea\u5668\u90fd\u5b9e\u73b0\u4e86\u81ea\u52a8\u5316\u3002<\/p>\n

3.3 \u591a\u8272\u591a\u53c2\u6570\u5206\u6790\u8fc5\u901f\u53d1\u5c55\uff0c\u5206\u6790\u3001\u5206\u7c7b\u901f\u5ea6\u52a0\u5feb<\/strong><\/p>\n

\u65b0\u5174\u8367\u5149\u63a2\u9488\u7684\u4e0d\u65ad\u5f00\u53d1\u548c\u4eea\u5668\u8f6f\u3001\u786c\u4ef6\u7684\u9010\u6b65\u66f4\u65b0\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u591a\u8272\u8367\u5149\u5206\u6790\u5f97\u5230\u4e86\u8fc5\u901f\u53d1\u5c55\uff0c\u5bf9\u7ec6\u80de\u4e9a\u7fa4\u7684\u8bc6\u522b\u66f4\u51c6\u786e\u3001\u66f4\u7cbe\u7ec6\u3002<\/p>\n

3.4 \u4eea\u5668\u5c0f\u578b\u5316<\/strong><\/p>\n

\u9002\u5e94\u5b9e\u9645\u9700\u8981\u7684\u5c0f\u578b\u5316\u3001\u4fbf\u643a\u5f0f\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e5f\u662f\u4e00\u4e2a\u53d1\u5c55\u7684\u8d8b\u52bf\u3002\u6700\u8fd1\u51e0\u5e74\uff0c\u5fae\u6d41\u4f53\u6d41\u5f0f\u7ec6\u80de\u4eea\u6210\u4e3a\u7814\u7a76\u7684\u70ed\u95e8\u65b9\u5411\uff0c\u5728\u8fd9\u65b9\u9762\u7684\u7814\u7a76\u4e5f\u8d8a\u6765\u8d8a\u591a\uff0c\u53d6\u5f97\u4e86\u4e00\u5b9a\u7684\u79d1\u7814\u6210\u679c\u3002<\/p>\n

3.5 \u4eea\u5668\u529f\u80fd\u589e\u5f3a\u5316<\/strong><\/p>\n

\u9274\u4e8e\u62c9\u66fc\u5149\u8c31\u6613\u4e8e\u8bc6\u522b\u7684\u7279\u70b9\uff0cDakota A. Watson \u7b49\u5bf9\u80fd\u591f\u63a2\u6d4b\u62c9\u66fc\u5149\u8c31\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fdb\u884c\u4e86\u7814\u7a76\uff0c\u8fd9\u5c06\u4f7f\u7ec6\u80de\u7684\u591a\u53c2\u6570\u6d4b\u91cf\u63d0\u5347\u4e00\u4e2a\u6863\u6b21\u3002<\/p>\n

3.6 \u7406\u8bba\u7684\u65b0\u53d1\u5c55<\/strong><\/p>\n

An-Shik Yang\u7b49\u91c7\u7528SIMPLEC\u8fed\u4ee3\u7b97\u6cd5\u5bf9\u6d41\u5f0f\u7ec6\u80de\u4eea\u6db2\u4f53\u6d41\u52a8\u7684\u7406\u8bba\u6a21\u62df\u7ed3\u679c\u4e0e\u6587\u732e\u516c\u5e03\u7684\u5b9e\u9a8c\u6570\u636e\u543b\u5408\u5f88\u597d\uff0c\u4e3a\u5fae\u6d41\u4f53\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u6d41\u4f53\u5ba4\u8bbe\u8ba1\u63d0\u4f9b\u4e86\u7406\u8bba\u53c2\u8003\u3002<\/p>\n

\u53e6\u5916\uff0c\u8bd5\u5242\u76d2\u8367\u5149\u8bd5\u5242\u3001\u6db2\u76f8\u82af\u7247\u6280\u672f\u3001\u5b9a\u91cf\u6d41\u5f0f\u7ec6\u80de\u5206\u6790\u3001\u4eea\u5668\u6a21\u5757\u5316\u7b49\u4e5f\u662f\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u53d1\u5c55\u8d8b\u52bf\u3002<\/p>\n

4 \u672a\u6765\u5c55\u671b<\/strong><\/span><\/p>\n

\u672c\u6587\u5bf9\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u539f\u7406\u3001\u5e94\u7528\u3001\u53d1\u5c55\u73b0\u72b6\u548c\u8d8b\u52bf\u4f5c\u4e86\u7b80\u660e\u627c\u8981\u7684\u8bba\u8ff0\uff0c\u4f7f\u79d1\u7814\u8005\u548c\u4f7f\u7528\u8005\u6709\u4e00\u4e2a\u66f4\u6df1\u523b\u7684\u4e86\u89e3\u548c\u8ba4\u8bc6\u3002\u968f\u7740\u79d1\u5b66\u6280\u672f\u7684\u8fdb\u6b65\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e13\u4e1a\u5316\u533a\u5206\u66f4\u52a0\u660e\u663e\uff0c\u79d1\u7814\u578b\u5927\u578b\u4eea\u5668\u548c\u666e\u53ca\u6027\u5e94\u7528\u4eea\u5668\u6210\u4e3a\u4e24\u4e2a\u4e3b\u6d41\uff1b\u968f\u7740\u5149\u7535\u6280\u672f\u7684\u53d1\u5c55\uff0c\u76f8\u4fe1\u6d41\u5f0f\u7ec6\u80de\u4eea\u80fd\u591f\u5728\u6d4b\u91cf\u591a\u8272\u8367\u5149\u53c2\u6570\u7684\u65f6\u5019\uff0c\u7ed9\u51fa\u591a\u53c2\u6570\u5206\u6790\u7ed3\u679c\uff0c\u540c\u65f6\u5b8c\u6210\u5206\u7c7b\uff0c\u5b9e\u73b0\u5b9e\u65f6\u540c\u6b65\u5206\u6790\u7684\u529f\u80fd\uff0c\u8fd9\u5bf9\u4e34\u5e8a\u533b\u7597\u5feb\u901f\u8bca\u65ad\u610f\u4e49\u91cd\u5927\u3002<\/p>\n

\u672c\u6587\u8f6c\u8f7d\u81ea\u4f5c\u8005\u8d75\u4e66\u6d9b\u7b49\u4eba\uff0c\u6750\u6599\u725b\u77f3\u5c0f\u6885\u7f16\u8f91\u6574\u7406\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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