{"id":109284,"date":"2017-11-02T06:30:16","date_gmt":"2017-11-01T22:30:16","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=109284"},"modified":"2017-10-31T19:19:12","modified_gmt":"2017-10-31T11:19:12","slug":"%e6%b9%96%e5%8d%97%e5%a4%a7%e5%ad%a6%e8%81%82%e8%88%9f%e8%af%be%e9%a2%98%e7%bb%84angew-chem-int-ed-%ef%bc%9a%e8%bd%ac%e8%82%bd%e9%85%b6%e7%9a%84%e5%a4%9a%e8%82%bd%e9%80%bb%e8%be%91%e9%97%a8","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=109284","title":{"rendered":"\u6e56\u5357\u5927\u5b66\u8042\u821f\u8bfe\u9898\u7ec4Angew. Chem. Int. Ed.\uff1a\u8f6c\u80bd\u9176\u7684\u591a\u80bd\u903b\u8f91\u95e8\u7535\u8def\u5b9e\u73b0\u903b\u8f91\u8c03\u63a7\u7ec6\u80de\u51cb\u4ea1"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u751f\u7269\u8ba1\u7b97\u4f5c\u4e3a\u4e00\u4e2a\u4fe1\u606f\u5904\u7406\u5de5\u5177\u5728\u591a\u53c2\u6570\u4f20\u611f\u548c\u751f\u7269\u533b\u5b66\u65b9\u9762\u663e\u793a\u5de8\u5927\u7684\u6f5c\u529b\u3002\u76ee\u524d\uff0c\u5927\u91cf\u591a\u6837\u7684\u590d\u6742\u751f\u7269\u903b\u8f91\u7cfb\u7edf\u53d1\u5c55\u8d77\u6765\uff0c\u4f46\u662f\u751f\u7269\u903b\u8f91\u8bbe\u5907\u5728\u64cd\u63a7\u7ec6\u80de\u884c\u4e3a\u65b9\u9762\u4f9d\u7136\u662f\u4e00\u5927\u6311\u6218\u3002DNA\u4f5c\u4e3a\u903b\u8f91\u95e8\u7684\u6784\u7b51\u6750\u6599\u5f15\u8d77\u4e86\u5e7f\u6cdb\u5173\u6ce8\uff0c\u4f46\u662f\u591a\u80bd\u903b\u8f91\u95e8\u8fd8\u5904\u5728\u8d77\u6b65\u9636\u6bb5\uff0c\u4e14\u6c28\u57fa\u9178\u6b8b\u57fa\u5177\u6709\u591a\u6837\u4e14\u590d\u6742\u7684\u5316\u5b66\u6027\u8d28\uff0c\u56e0\u6b64\u4e9f\u9700\u5f00\u53d1\u4e00\u79cd\u901a\u7528\u578b\u7684\u591a\u80bd\u751f\u7269\u903b\u8f91\u7cfb\u7edf\uff0c\u5b9e\u73b0\u667a\u80fd\u8c03\u63a7\u7ec6\u80de\u884c\u4e3a\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u6700\u8fd1\uff0c\u6e56\u5357\u5927\u5b66\u8042\u821f\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u8bfe\u9898\u7ec4\u62a5\u9053\u4e86\u4e00\u4e2a\u65b0\u9896\u7684\u591a\u80bd\u751f\u7269\u903b\u8f91\u7cfb\u7edf\uff0c\u901a\u8fc7\u5229\u7528\u8f6c\u80bd\u9176A\uff08Srt A\uff09\u4f5c\u4e3a\u4e00\u79cd\u901a\u7528\u5904\u7406\u5668\u6765\u8c03\u8282\u7ec6\u80de\u529f\u80fd\u884c\u4e3a\u3002\u91cd\u8981\u7684\u662f\uff0c\u901a\u8fc7\u6a21\u5757\u5316\u7684\u591a\u80bd\u8bbe\u8ba1\uff0c\u6210\u529f\u6784\u5efa\u4e86\u590d\u6742\u7684\u529f\u80fd\u903b\u8f91\u5668\u4ef6\uff0c\u5982\u591a\u80bd\u5bc6\u7801\u9501\uff0c\u5b9e\u73b0\u903b\u8f91\u8c03\u63a7\u7ec6\u80de\u51cb\u4ea1\uff1b\u6b64\u5916\uff0c\u5e76\u5efa\u7acb\u4e86\u4e00\u5957\u6982\u5ff5\u9a8c\u8bc1\u7684\u591a\u80bd\u8c03\u63a7\u7535\u8def\u5b9e\u73b0\u5206\u6790\u7ec6\u80de\u5206\u6ccc\u86cb\u767d\u751f\u7269\u6807\u5fd7\u7269\u7684\u8868\u8fbe\u53ca\u89e6\u53d1\u7279\u5f02\u6027\u7684\u7ec6\u80de\u51cb\u4ea1\u3002\u8be5\u6210\u679c\u4ee5\u201c Peptide Logic Circuits Based on Chemoenzymatic Ligation for Programmable Cell Apoptosis<\/strong>\u201d\u53d1\u8868\u5728Angew. Chem. Int. Ed.<\/strong>\u9876\u7ea7\u671f\u520a\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/strong><\/p>\n

\u793a\u610f\u56fe1 \u7ec6\u80de\u51cb\u4ea1\u4f5c\u4e3a\u8f93\u51fa\u4fe1\u53f7\u591a\u80bd\u903b\u8f91\u7cfb\u7edf\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u793a\u610f\u56fe1 \u7ec6\u80de\u51cb\u4ea1\u4f5c\u4e3a\u8f93\u51fa\u4fe1\u53f7\u7684\u8f6c\u80bd\u9176\u591a\u80bd\u751f\u7269\u8fd0\u7b97\u793a\u610f\u56fe<\/p>\n

\u56fe1 SrtA\u5904\u7406\u7684\u591a\u80bd\u903b\u8f91\u8c03\u8282\u764c\u75c7\u7ec6\u80de\u51cb\u4ea1\u7684\u8fc7\u7a0b<\/strong><\/p>\n

\"\"<\/p>\n

(a) AND\u903b\u8f91\u95e8\u793a\u610f\u56fe\u3002<\/p>\n

(b) \u6fc0\u5149\u5171\u805a\u7126\u7ec6\u80de\u6210\u50cf\u56fe\u3002<\/p>\n

(c) Annexin V-FITC (\u7eff\u8272)\/PI(\u7ea2\u8272) \u53cc\u67d3\u8272\u6cd5\u7684\u6d41\u5f0f\u7ec6\u80de\u5206\u6790\u56fe\u3002<\/p>\n

(d) caspase 3 \u6fc0\u6d3b\u7684Hela\u7ec6\u80de\u4e0e\u8f93\u5165\u591a\u80bd\u5171\u5b75\u80b2\u4e4b\u540e\u7684\u514d\u75ab\u5370\u8ff9\u5206\u6790\u56fe\u3002<\/p>\n

(e) \u80bf\u7624\u5c3a\u5bf8\u53d8\u5316\u56fe\u3001\u663e\u5fae\u955c\u6210\u50cf\u56fe\u548c\u8367\u5149\u6210\u50cf\u56fe\u3002<\/p>\n

\u56fe2 SrtA\u7684\u591a\u80bd\u4e32\u8054\u903b\u8f91\u8c03\u8282\u764c\u75c7\u7ec6\u80de\u51cb\u4ea1\u7684\u8fc7\u7a0b<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u4e32\u8054\u903b\u8f91\u95e8\u7684\u4e09\u80bd\u952e\u76d8\u9501\u56fe\u3002<\/p>\n

(b) \u4e09\u8f93\u5165AND\u903b\u8f91\u95e8\u7684\u903b\u8f91\u7b26\u53f7\u548c\u7ec6\u80de\u51cb\u4ea1\u67f1\u72b6\u56fe\u3002<\/p>\n

(c) \u4e09\u4f4d\u952e\u76d8\u9501\u7684\u7ec6\u80de\u51cb\u4ea1\u7387\u7684\u6240\u6709\u6392\u5217\u56fe\u3002<\/p>\n

(d) \u80bd\u952e\u76d8\u9501\u8c03\u63a7\u80bf\u7624\u51cb\u4ea1\u7684\u80bf\u7624\u5c3a\u5bf8\u3001\u663e\u5fae\u955c\u6210\u50cf\u548c\u8367\u5149\u6210\u50cf\u56fe\u3002<\/p>\n

\u56fe3 SrtA\u903b\u8f91\u95e8\u88c5\u7f6e\u5b9e\u73b0\u764c\u75c7\u7ec6\u80de\u8bc6\u522b\u548c\u7279\u5f02\u6027\u8c03\u63a7\u7ec6\u80de\u51cb\u4ea1<\/strong><\/p>\n

\"\"<\/p>\n

(a) SrtA\u903b\u8f91\u95e8\u8bbe\u5907\u7684\u8ba1\u7b97\u5904\u7406\u8fc7\u7a0b\u56fe\u3002<\/p>\n

(b) Annexin V-FITC (\u7eff\u8272)\/PI(\u7ea2\u8272) \u53cc\u67d3\u8272\u7684\u56db\u79cd\u764c\u7ec6\u80de\u7cfb\uff08HepG2\u3001ES\u3001HSAS3\u3001LO2\uff09\u7684\u6fc0\u5149\u5171\u805a\u7126\u6210\u50cf\u56fe\u3002<\/p>\n

(c) \u56db\u79cd\u7ec6\u80de\u4e0e\u6bcf\u4e2a\u903b\u8f91\u95e8\u591a\u80bd\u5171\u5b75\u80b2\u4e4b\u540e\u7684\u7ec6\u80de\u51cb\u4ea1\u6bd4\u70ed\u56fe\u3002<\/p>\n

\u3010\u5c55\u671b\u3011<\/strong><\/p>\n

\u8be5\u5de5\u4f5c\u9996\u6b21\u6784\u5efa\u4e86\u4e00\u79cd\u901a\u7528\u578b\u3001\u591a\u529f\u80fd\u6a21\u5757\u5316\u7684\u591a\u80bd\u903b\u8f91\u7cfb\u7edf\uff0c\u5229\u7528\u8f6c\u80bd\u9176A\u6784\u5efa\u591a\u80bd\u903b\u8f91\u95e8\u5b9e\u73b0\u7ec6\u80de\u5185\u529f\u80fd\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u901a\u8fc7\u6a21\u5757\u5316\u7684\u8bbe\u8ba1\uff0c\u660e\u663e\u589e\u5f3a\u4e86\u591a\u80bd\u7535\u8def\u7684\u590d\u6742\u6027\u548c\u529f\u80fd\u6027\uff0c\u4e14\u8fd9\u79cd\u6982\u5ff5\u9a8c\u8bc1\u8bbe\u5907\u5b9e\u73b0\u4e86\u7ec6\u80de\u7684\u8bc6\u522b\u548c\u76ee\u6807\u7ec6\u80de\u7684\u6740\u4f24\u3002\u8fd9\u4e00\u7b56\u7565\u4e3a\u591a\u80bd\u751f\u7269\u8ba1\u7b97\u7cfb\u7edf\u7684\u7eb3\u7c73\u5668\u4ef6\u3001\u7ec6\u80de\u7f16\u7a0b\u3001\u75be\u75c5\u8bca\u65ad\u548c\u6cbb\u7597\u5f00\u8f9f\u4e86\u65b0\u7684\u9053\u8def\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/b><\/p>\n

Peptide\u00a0Logic\u00a0Circuits\u00a0Based\u00a0on\u00a0Chemoenzymatic\u00a0Ligation\u00a0for\u00a0Programmable\u00a0Cell\u00a0Apoptosis<\/b><\/a><\/span>\u00a0<\/b>(\u00a0Angew.\u00a0Chem.\u00a0Int.\u00a0Ed,\u00a02017,\u00a0DOI:\u00a010.1002\/anie.201708327)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u751f\u7269\u6750\u6599\u7ec4\u9093\u5b8f\u534e\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

\u53c2\u4e0e\u751f\u7269\u6750\u6599\u8bdd\u9898\u8ba8\u8bba\u6216\u4e86\u89e3\u751f\u7269\u6750\u6599\u5c0f\u7ec4\u62db\u52df\u8be6\u60c5\uff0c\u8bf7\u52a0\u5165\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u4ea4\u6d41\u7fa4\uff08124806506\uff09\u3002<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1aRDD-2011-CHERISH\uff0c\u4efb\u4e39\u4e39\uff0c\u6211\u4eec\u4f1a\u9080\u8bf7\u5404\u4f4d\u8001\u5e08\u52a0\u5165\u4e13\u5bb6\u7fa4\u3002<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c<\/b>\u4e0a\u6d4b\u8bd5\u8c37<\/b><\/a>\uff01<\/b><\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u751f\u7269\u8ba1\u7b97\u4f5c\u4e3a\u4e00\u4e2a\u4fe1\u606f\u5904\u7406\u5de5\u5177\u5728\u591a\u53c2\u6570\u4f20\u611f\u548c\u751f\u7269\u533b\u5b66\u65b9\u9762\u663e\u793a\u5de8\u5927\u7684\u6f5c\u529b\u3002\u76ee\u524d\uff0c\u5927\u91cf\u591a\u6837\u7684\u590d\u6742\u751f\u7269\u903b\u8f91\u7cfb\u7edf\u53d1\u5c55\u8d77\u6765\uff0c\u4f46\u662f\u751f\u7269\u903b\u8f91\u8bbe\u5907\u5728\u64cd\u63a7\u7ec6\u80de\u884c\u4e3a\u65b9\u9762\u4f9d\u7136…<\/p>\n","protected":false},"author":1745,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[75],"class_list":["post-109284","post","type-post","status-publish","format-standard","hentry","category-jiedu","tag-biomaterial"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/109284"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/1745"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=109284"}],"version-history":[{"count":5,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/109284\/revisions"}],"predecessor-version":[{"id":109556,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/109284\/revisions\/109556"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=109284"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=109284"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=109284"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}