{"id":117556,"date":"2017-12-20T06:30:38","date_gmt":"2017-12-19T22:30:38","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=117556"},"modified":"2017-12-18T20:06:26","modified_gmt":"2017-12-18T12:06:26","slug":"%e4%bf%84%e4%ba%a5%e4%bf%84%e5%b7%9e%e7%ab%8b%e5%a4%a7%e5%ad%a6nature-nanotechnology%ef%bc%9a%e9%80%9a%e8%bf%87%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90%e6%96%b9%e5%90%91%e6%80%a7%e6%8e%a7%e5%88%b6rna","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=117556","title":{"rendered":"\u4fc4\u4ea5\u4fc4\u5dde\u7acb\u5927\u5b66Nature Nanotechnology\uff1a\u901a\u8fc7\u7eb3\u7c73\u7c92\u5b50\u65b9\u5411\u6027\u63a7\u5236RNA\u53ca\u76f8\u5e94\u914d\u4f53\u8fbe\u5230\u6cbb\u7597\u764c\u75c7\u7684\u6548\u679c"},"content":{"rendered":"<p><strong>\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n<p>\u7eb3\u7c73\u6750\u6599\u662f80\u5e74\u4ee3\u4e2d\u671f\u53d1\u5c55\u8d77\u6765\u7684\u65b0\u578b\u6750\u6599\u3002\u7eb3\u7c73\u6750\u6599\u5177\u6709\u8bb8\u591a\u72ec\u7279\u529f\u80fd\uff0c\u800c\u4e14\u7528\u91cf\u5c11\uff0c\u4f46\u5374\u8d4b\u4e88\u6750\u6599\u610f\u60f3\u4e0d\u5230\u7684\u9ad8\u6027\u80fd\uff0c\u9644\u52a0\u503c\u751a\u9ad8\u3002\u7eb3\u7c73\u590d\u5408\u9ad8\u5206\u5b50\u6750\u6599\u3001\u7eb3\u7c73\u6297\u83cc\u3001\u4fdd\u9c9c\u3001\u9664\u81ed\u6750\u6599\u7b49\u7b49\uff0c\u7531\u4e8e\u7eb3\u7c73\u6750\u6599\u7684\u5c3a\u5bf8\u5c0f\uff0c\u6bd4\u8840\u6db2\u4e2d\u7684\u7ea2\u8840\u7403\u5c0f\u4e00\u5343\u591a\u500d\uff0c\u6bd4\u7ec6\u83cc\u5c0f\u51e0\u5341\u500d\uff0c\u6c14\u4f53\u901a\u8fc7\u5176\u6269\u6563\u7684\u901f\u5ea6\u6bd4\u5e38\u89c4\u6750\u6599\u5feb\u51e0\u5343\u500d\u3002\u7eb3\u7c73\u9897\u7c92\u4e0e\u751f\u7269\u7ec6\u80de\u819c\u7684\u5316\u7269\u4f5c\u7528\u5f88\u5f3a\uff0c\u6781\u6613\u8fdb\u5165\u7ec6\u80de\u5185\u3002\u6b64\u7bc7\u6587\u7ae0\u62a5\u9053\u4e86\u7eb3\u7c73\u6280\u672f\u5bf9RNA\u65b9\u5411\u63a7\u5236\u7684\u4f18\u52bf\u3002<\/p>\n<p><strong>\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u4fc4\u4ea5\u4fc4\u5dde\u7acb\u5927\u5b66\u7684\u7f8e\u56fdNIH\u7eb3\u7c73\u533b\u5b66\u4e2d\u5fc3\u4e3b\u4efb\u90ed\u57f9\u5ba3\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u7b49\u4eba\u5728<strong>Nature Nanotechnology<\/strong>\u4e0a\u53d1\u8868\u6700\u65b0\u7814\u7a76\u6210\u679c\u201c<strong>Nanoparticle orientation to control RNA loading and ligand display on extracellular vesicles for cancer regression<\/strong>\u201d\u3002\u5728\u8be5\u6587\u4e2d\uff0c\u7814\u7a76\u56e2\u961f\u62a5\u9053\u4e86\u5e94\u7528\u7eb3\u7c73\u6280\u672f\u6765\u63a7\u5236RNA\u65b9\u5411\u7684\u4f18\u52bf\u6027\u3002\u5b9e\u9a8c\u901a\u8fc7\u6539\u53d8\u7bad\u5934\u72b6RNA\u7684\u65b9\u5411\u6765\u63a7\u5236\u7ec6\u80de\u5916\u56ca\u6ce1\u819c\u4e0a\u7684\u914d\u4f53\uff0c\u6216\u8c03\u63a7\u5e72\u6270\u5c0fRNA\u3001\u5faeRNA\u7684\u7ec6\u80de\u5185\u8fd0\u8f93\u3002\u5728\u7bad\u5934\u72b6RNA\u7684\u5c3e\u90e8\u653e\u7f6e\u819c\u951a\u5b9a\u80c6\u56fa\u9187\u4f7f\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u5916\u8868\u9762\u4e0a\u51fa\u73b0RNA\u9002\u4f53\u6216\u53f6\u9178\u3002\u5229\u7528\u5177\u6709\u7279\u5b9a\u9776\u5411\u7684RNA\u914d\u4f53\u4e0e\u7ec6\u80de\u5916\u56ca\u6ce1\u8fdb\u884c\u6709\u6548\u878d\u5408\uff0c\u7531\u6b64\u5f97\u5230\u7684\u914d\u4f53\u80fd\u591f\u5c06siRNA\u7cbe\u51c6\u9012\u9001\u81f3\u7ec6\u80de\uff0c\u5e76\u6709\u6548\u5730\u963b\u65ad\u4e09\u79cd\u764c\u75c7\u6a21\u578b\u4e2d\u7684\u80bf\u7624\u751f\u957f\u3002<\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>\u56fe1 \u4fee\u9970\u539f\u751f\u7ec6\u80de\u5916\u56ca\u6ce1\u7684RNA\u7eb3\u7c73\u6280\u672f\u00a0<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-117557 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/1-59.jpg\" alt=\"\" width=\"750\" height=\"809\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/1-59.jpg 750w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/1-59-278x300.jpg 278w\" sizes=\"(max-width: 750px) 100vw, 750px\" \/><\/p>\n<p>a) \u566c\u83cc\u4f53phi29\u7684\u6269\u5c55\u4e09\u901a\u8fde\u63a5\u9a6c\u8fbepRNA\u7684\u539f\u5b50\u529b\u663e\u5fae\u955c\u56fe\u50cf\uff0c\u8272\u6807\u8868\u793a\u5e45\u5ea6\u53d8\u5316\uff1b<\/p>\n<p>b) 3WJ\u7bad\u5934\u6216\u7bad\u5934\u72b6\u80c6\u56fa\u9187\u6807\u8bb0\u4f4d\u7f6e\u793a\u610f\u56fe\uff1b<\/p>\n<p>c) \u901a\u8fc7\u5dee\u901f\u8d85\u901f\u79bb\u5fc3\uff08UC\uff09\u65b9\u6cd5\u548c\u7f13\u51b2\u57ab\u4fee\u9970\u8d85\u901f\u79bb\u5fc3\u6cd5\u7eaf\u5316\u7684HEK293T\u7ec6\u80de\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u8d1f\u67d3\u7535\u5b50\u663e\u5fae\u955c\uff08EM\uff09\u56fe\u50cf\uff1b<\/p>\n<p>d-g) \u7528\u7eb3\u7c73\u7c92\u5b50\u8ddf\u8e2a\u5206\u6790\u65b9\u6cd5\u8fdb\u884c\u7684\u5c3a\u5bf8\u5206\u6790\u793a\u610f\u56fe\u548c\u7528\u4e8ezeta\u7535\u4f4d\u6d4b\u91cf\u7684\u52a8\u6001\u5149\u6563\u5c04\u793a\u610f\u56fe\uff1b<\/p>\n<p>h) \u4e8c\u7ef4\u7ed3\u6784\uff08\u5de6\u56fe\uff09\u548c\u539f\u751fPAGE\uff0c\u7528\u4e8e\u4ece\u4e09\u4e2a\u7ec4\u5206\u94fe\u7ec4\u88c5\u76843WJ \u793a\u610f\u56fe\uff1b<\/p>\n<p>i) \u7ec6\u80de\u5916\u56ca\u6ce1\u8d1f\u8f7d\u4e0eRNA\u9002\u4f53\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p><strong>\u56fe2 \u7bad\u5934\u72b6RNA\u548c\u7bad\u5934\u72b63WJ\u7684\u4f5c\u7528\u6bd4\u8f83<\/strong>\u00a0<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-117558 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/2-49.jpg\" alt=\"\" width=\"718\" height=\"520\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/2-49.jpg 718w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/2-49-300x217.jpg 300w\" sizes=\"(max-width: 718px) 100vw, 718px\" \/><\/p>\n<p>a,b) \u7bad\u5934\u72b6\u7ec6\u80de\u5916\u56ca\u6ce1\u548c\u7bad\u5934\u72b63WJ\u7684\u533a\u522b\u793a\u610f\u56fe\uff1b<\/p>\n<p>c,d) \u7bad\u5934\u72b6\u548c\u7bad\u5934\u72b6Alexa647-3WJ\/EV\u5728\u725b\u80ce\u8840\u6e05\u4e2d\u6838\u7cd6\u6838\u9178\u9176\u7684\u964d\u89e3\u60c5\u51b5\uff0c\u51dd\u80f6\u5728Alexa647\u7684\u901a\u9053\u4e0a\u6210\u50cf\uff0c\u51dd\u80f6\u6210\u50cf\uff08c\uff09\u4ea7\u751f\u7684\u6761\u5e26\u901a\u8fc7\u56fe\u50cf\uff08d\uff09\u5b9a\u91cf\u8868\u793a\uff1b<\/p>\n<p>e-i) \u6bd4\u8f83\u53d7\u4f53\u9633\u6027\u548c\u9634\u6027\u7ec6\u80de\u4e2d\u7bad\u5934\u72b6\u53f6\u9178-3WJ\u7684\u7ec6\u80de\u7ed3\u5408\u80fd\u529b\uff1b<\/p>\n<p>e-g) \u6bd4\u8f83\u53d7\u4f53\u9633\u6027\u548c\u9634\u6027\u7ec6\u80de\u4e2d\u7bad\u5934\u72b6\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u7ec6\u80de\u7ed3\u5408\u80fd\u529b\uff1b<\/p>\n<p>h,i) \u6bd4\u8f83\u53d7\u4f53\u9633\u6027\u548c\u9634\u6027\u7ec6\u80de\u4e2d\u53f6\u9178\u7684\u7ec6\u80de\u7ed3\u5408\u80fd\u529b\u3002* p &lt;0.05\u3002<\/p>\n<p><strong>\u56fe3 \u542bPSMA\u9002\u4f53\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u5728\u4f53\u5916\u8fdb\u884c\u7279\u5f02\u6027\u7ed3\u5408\u4e0esiRNA\u7684\u9012\u9001<\/strong>\u00a0<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-117559 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/3-53.jpg\" alt=\"\" width=\"707\" height=\"408\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/3-53.jpg 707w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/3-53-300x173.jpg 300w\" sizes=\"(max-width: 707px) 100vw, 707px\" \/><\/p>\n<p>a) PSMA RNA\u9002\u4f53\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u4e0ePSMA\u53d7\u4f53\u9633\u6027\u548c\u9634\u6027\u7ec6\u80de\u7ed3\u5408\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\uff08\u5de6\uff09\u548c\u5171\u7126\u56fe\u50cf\uff08\u53f3\uff09\u3002\u5171\u805a\u7126\u56fe\u50cf\u4e2d\u7684\u6838\uff08\u84dd\u8272\uff09\uff0c\u7ec6\u80de\u9aa8\u67b6\uff08\u7eff\u8272\uff09\uff0cRNA\uff08\u7ea2\u8272\uff09\uff1b<\/p>\n<p>b) \u901a\u8fc7\u7ec6\u80de\u5916\u56ca\u6ce1\u5411PSMA+\u524d\u5217\u817a\u764c\u7ec6\u80de\u7684PSMA-\u9002\u4f53\u4ecb\u5bfc\u9012\u9001\u751f\u5b58\u7d20siRNA\u7684RT-PCR\u6d4b\u5b9a\u3002\u7edf\u8ba1\uff1an=4\uff1b\u5b9e\u9a8c\u7528\u56db\u4e2a\u751f\u7269\u5b66\u91cd\u590d\u548c\u4e8c\u5230\u56db\u4e2a\u6280\u672f\u91cd\u590d\u8fdb\u884cANOVA\u5206\u6790\uff1b\u6821\u6b63\uff1ap=0.0120,0.0067\uff0c\u5206\u522b\u6bd4\u8f83PSMAapt\/EV\/siSurvivin\u548cPSMAapt\/EV\/siScramble\u548c3WJ\/EV\/siSurvivin\uff1b<\/p>\n<p>c) \u5bf9\u51cf\u5c11\u7684\u7ec6\u80de\u8fdb\u884cMTT\u6d4b\u5b9a\u3002n=3\uff0cp=0.003,0.031\uff0c\u5206\u522b\u6bd4\u8f83PSMAapt\/EV\/siSurvivin\u4e0ePSMAapt\/EV\/siScramble\u548c3WJ\/EV\/siSurvivin\u3002* p &lt;0.05\uff0c** p &lt;0.01\u3002<\/p>\n<p><strong>\u56fe4 \u4f7f\u7528\u542b\u914d\u4f53\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u6291\u5236\u80bf\u7624\u751f\u957f<\/strong>\u00a0<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-117560 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/4-47.jpg\" alt=\"\" width=\"629\" height=\"517\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/4-47.jpg 629w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2017\/12\/4-47-300x247.jpg 300w\" sizes=\"(max-width: 629px) 100vw, 629px\" \/><\/p>\n<p>a) \u5668\u5b98\u56fe\u50cf\u663e\u793a\u5728\u5c06\u76ae\u4e0b\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u5168\u8eab\u6027\u53f6\u9178\u6ce8\u5c04\u5230\u5177\u6709\u76ae\u4e0bKB\u7ec6\u80de\u5f02\u79cd\u79fb\u690d\u7269\u7684\u5c0f\u9f208\u5c0f\u65f6\u540e\u7684\u7279\u5f02\u6027\u80bf\u7624\u9776\u5411\u3002n=2\uff0c\u4e24\u4e2a\u72ec\u7acb\u7684\u5b9e\u9a8c\uff1b<\/p>\n<p>b) \u7528\u9759\u8109\u5185\u5904\u7406\u643a\u5e26\u76ae\u4e0bLNCaP-LN3\u4e14\u5f02\u79cd\u79fb\u690dPSMAapt\/EV\/siSurvivin\u6216PSMAapt\/EV\/siScramble\uff08\u5747\u5177\u67090.6mg\/kg\uff0csiRNA\/\u5c0f\u9f20\u4f53\u91cd\uff09\u7684\u88f8\u9f20\uff0c\u4ee5\u6ce8\u5c04PBS\u4e3a\u5bf9\u7167\uff0c\u6bcf\u5468\u6ce8\u5c04\u4e24\u6b21\uff0c\u6ce8\u5c04\u4e09\u4e2a\u661f\u671f\u3002\u751f\u7269\u5b66\u91cd\u590dn=10\uff0c\u4e24\u4e2a\u72ec\u7acb\u7684\u5b9e\u9a8c\uff0c\u5e76\u4e14\u7edf\u8ba1\u6570\u636e\u4f7f\u7528\u4ee5\u5e73\u5747\u503c\u548c\u6807\u51c6\u504f\u5dee\u8868\u793a\u7684\u53cc\u4fa7t-\u68c0\u9a8c\u6765\u8ba1\u7b97\u3002\u7b2c15,18\u5929\u7684p=0.347,0.6\u00d710-2,1.5\u00d710-6,8.2\u00d710-8,2.1\u00d710-7,1.0\u00d710-7,1.9\u00d710-7\u548c1.8\u00d710-6\uff0c22,25,29,32,36\u548c39\u5206\u522b\u8868\u793aPSMAapt\/EV\/siSurvivin\u548c\u5bf9\u7167\u7ec4\uff1b<\/p>\n<p>c) \u7528\u7ec6\u80de\u5916\u56ca\u6ce1\u6cbb\u7597\u671f\u95f4\u5c0f\u9f20\u7684\u4f53\u91cd\uff1b<\/p>\n<p>d) 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\u6709EGFR\u9002\u4f53\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u5bf9\u539f\u4f4d\u5f02\u79cd\u79fb\u690d\u5c0f\u9f20\u6a21\u578b\u4e2d\u4e73\u817a\u80bf\u7624\u6709\u589e\u5f3a\u7684\u9776\u5411\u4f5c\u7528\u3002\u8272\u6807\u8868\u793a\u201c\u65b9\u6cd5\u201d\u4e2d\u5b9a\u4e49\u7684\u8f90\u5c04\u6548\u7387\uff1b<\/p>\n<p>b) \u7528EGFRapt\/EV\/siSurvivin\u548c\u5bf9\u7167FBS\uff08n=5\uff09\u9759\u8109\u5185\u5904\u7406\u643a\u5e26\u4e73\u817a\u764c\u539f\u4f4d\u5f02\u79cd\u79fb\u690d\u7269\u7684\u88f8\u9f20\u30026\u5468\u540e\uff0cEGFRapt\/EV\/siSurvivin\u6cbb\u7597\u7ec4\u80bf\u7624\u5927\u5c0f\u660e\u663e\u5c0f\u4e8e\u5176\u4ed6\u5bf9\u7167\u7ec4\u3002\u5c06EGFRapt\/EV\/siSurvivin\u4e0eEGFRapt\/EV\/siScramble\u8fdb\u884c\u6bd4\u8f83\uff0cp=0.008\uff1b<\/p>\n<p>c) 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href=\"https:\/\/www.nature.com\/articles\/s41565-017-0012-z\" target=\"_blank\" rel=\"noopener\">Nanoparticle orientation to control RNA loading and ligand display on extracellular vesicles for cancer regression<\/a>\uff08Nature Nanotechnology\uff0c2017\uff0cDOI\uff1a10.1038\/s41565-017-0012-z\uff09<\/p>\n<p>\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u751f\u7269\u6750\u6599\u5b66\u672f\u7ec4\u661d\u83f2\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n<p>\u6750\u6599\u4eba\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c<a 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