{"id":120621,"date":"2018-01-10T14:18:51","date_gmt":"2018-01-10T06:18:51","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=120621"},"modified":"2018-01-24T10:49:37","modified_gmt":"2018-01-24T02:49:37","slug":"%e8%8b%8f%e5%b7%9e%e5%a4%a7%e5%ad%a6%e9%ab%98%e6%98%8e%e8%bf%9c%e6%95%99%e6%8e%88%e5%9b%a2%e9%98%9fjacs%ef%bc%9a%e5%8f%8c%e6%af%94%e7%8e%87%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88%e5%ae%9e%e7%8e%b0","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=120621","title":{"rendered":"\u4e2d\u79d1\u9662\u5316\u5b66\u6240\u9ad8\u660e\u8fdcJACS\uff1a\u53cc\u6bd4\u7387\u8367\u5149\u63a2\u9488\u5b9e\u73b0\u6d3b\u4f53MMP-9\u548cpH\u53d8\u5316\u7684\u5b9e\u65f6\u76d1\u6d4b"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u8fd1\u6765\uff0c\u80bf\u7624\u5fae\u73af\u5883\u5728\u80bf\u7624\u7684\u590d\u53d1\u548c\u8f6c\u79fb\u4e2d\u7684\u4f5c\u7528\u53d7\u5230\u80bf\u7624\u7814\u7a76\u8005\u7684\u9ad8\u5ea6\u5173\u6ce8\u3002\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u5145\u65a5\u7740\u5927\u91cf\u8bf8\u5982\u57fa\u8d28\u91d1\u5c5e\u86cb\u767d\u9176\uff08MMPs\uff09\u7684\u86cb\u767d\u9176\u7c7b\u5bb6\u65cf\uff0cMMPs\u4e0d\u4ec5\u548c\u80bf\u7624\u8f6c\u79fb\u76f8\u5173\uff0c\u8fd8\u4e0e\u7ec6\u80de\u51cb\u4ea1\u3001\u8840\u7ba1\u751f\u6210\u3001\u80bf\u7624\u751f\u957f\u6709\u5173\u3002\u901a\u5e38MMPs\u7684\u68c0\u6d4b\u662f\u5229\u7528\u5c0f\u7ec4\u7ec7\u6d3b\u68c0\u6216\u4f53\u5185\u8367\u5149\u7b49\u65b9\u5f0f,\u5c0f\u7ec4\u7ec7\u6d3b\u68c0\u4e0d\u80fd\u5b8c\u5168\u53cd\u5e94MMPs\u5728\u80bf\u7624\u4e2d\u7684\u6574\u4f53\u5206\u5e03\uff0c\u4f53\u5185\u8367\u5149\u65b9\u5f0f\u4e0d\u80fd\u63d0\u4f9bMMPs\u8868\u8fbe\u7684\u5b9a\u91cf\u4fe1\u606f\u3002<\/p>\n

\u5728\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u9664\u4e86MMP\u7684\u5f02\u5e38\u8868\u8fbe\u5916\uff0c\u7531\u8461\u8404\u7cd6\u4ee3\u8c22\u6539\u53d8\u548c\u8461\u8404\u7cd6\u6444\u53d6\u589e\u52a0\u5f15\u8d77\u7684pH\u503c\u964d\u4f4e\u4e5f\u662f\u80bf\u7624\u7684\u53e6\u4e00\u4e2a\u91cd\u8981\u7279\u5f81\u3002\u7136\u800c\u5e38\u7528\u7684pH\u54cd\u5e94\u6027\u8367\u5149\u63a2\u9488\u901a\u5e38\u4f1a\u53d7\u5230\u8bb8\u591a\u5916\u754c\u56e0\u7d20\u7684\u5e72\u6270\uff0c\u800c\u5f71\u54cd\u6d3b\u4f53\u80bf\u7624pH\u7684\u68c0\u6d4b\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u6bd4\u7387\u578b\u8367\u5149\u63a2\u9488\u53ef\u4ee5\u5f88\u597d\u7684\u7f13\u89e3\u5916\u754c\u5e72\u6270\uff0c\u5e76\u53ef\u5b9e\u73b0pH\u7684\u5b9a\u91cf\u68c0\u6d4b\u3002\u7136\u800c\uff0c\u76ee\u524d\u7814\u7a76\u7684\u6210\u50cf\u63a2\u9488\u4ec5\u53ef\u7528\u4e8e\u80bf\u7624\u5fae\u73af\u5883\u5355\u4e00\u65b9\u9762\u5f02\u5e38\u56e0\u7d20\u7684\u68c0\u6d4b\uff0c\u4f46\u4e0e\u80bf\u7624\u76f8\u5173\u7684\u5f02\u5e38\u6539\u53d8\u901a\u5e38\u662f\u591a\u65b9\u9762\u5e76\u4e14\u76f8\u4e92\u4e4b\u95f4\u662f\u5f3a\u70c8\u76f8\u5173\u7684\u3002\u56e0\u6b64\uff0c\u5bf9\u4e8e\u53ef\u540c\u65f6\u68c0\u6d4b\u80bf\u7624\u76f8\u5173\u86cb\u767d\u9176\uff08\u5982MMPs\uff09\u548c\u7ec6\u80de\u5916pH\u503c\u7684\u63a2\u9488\u7684\u7814\u7a76\u6210\u4e3a\u5f53\u4e0b\u4e9f\u5f85\u89e3\u51b3\u7684\u7814\u7a76\u70ed\u70b9\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u4e2d\u79d1\u9662\u5316\u5b66\u6240\u9ad8\u660e\u8fdc\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u56e2\u961f\u5728JACS<\/strong>\u5728\u7ebf\u53d1\u8868\u4e86\u4e00\u7bc7\u9898\u4e3a\u201cDual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH in Vivo<\/strong>\u201d\u7684\u6587\u7ae0\uff0c\u4ecb\u7ecd\u4e86\u4e00\u79cd\u53cc\u6bd4\u7387\u8367\u5149\u63a2\u9488\uff0c\u5b9e\u73b0\u4e86MMP-9\u8fc7\u8868\u8fbe\u548c\u80bf\u7624\u5fae\u73af\u5883pH\u5f02\u5e38\u7684\u5b9a\u91cf\u53ef\u89c6\u5316\uff0c\u9610\u660e\u5176\u7a7a\u95f4\u5f02\u8d28\u6027\u5e76\u4e14\u534f\u540c\u7684\u6307\u793a\u4e86\u4f53\u5185\u80bf\u7624\u5165\u4fb5\u8fc7\u7a0b\u3002\u8be5\u7814\u7a76\u6700\u5927\u7684\u521b\u65b0\u70b9\u5728\u4e8e\u901a\u8fc7\u65e0\u521b\u4f24\u591a\u6a21\u6001\u6210\u50cf\u5bf9\u591a\u79cd\u80bf\u7624\u5fae\u73af\u5883\u7279\u5f81\u4fe1\u53f7\u8fdb\u884c\u5b9a\u91cf\u548c\u5b9e\u65f6\u68c0\u6d4b\u3002\u7814\u7a76\u8005\u5bf9\u6240\u8bbe\u8ba1\u63a2\u9488\u4e00\u822c\u6982\u5ff5\u7684\u5c55\u793a\u53ef\u80fd\u4e3a\u4f53\u5185\u5f02\u5e38\u80bf\u7624\u7279\u5f81\u7814\u7a76\u63d0\u4f9b\u4e86\u4e00\u79cd\u5f3a\u6709\u529b\u5de5\u5177\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u793a\u610f\u56fe<\/strong> \u9759\u8109\u6ce8\u5c04\u7eb3\u7c73\u63a2\u9488\u540e\u63a2\u9488\u901a\u8fc7\u53d7\u635f\u8840\u7ba1\u8fdb\u5165\u80bf\u7624\u7ec4\u7ec7<\/strong><\/p>\n

\"\"\u7eb3\u7c73\u63a2\u9488\u4e00\u65e6\u8fdb\u5165\u80bf\u7624\u7ec4\u7ec7\u5185\uff0cMMP-9\u5c06\u5bf9\u8fde\u63a5ANNA\uff08pH\u654f\u611f\u7684\u8367\u5149\u67d3\u6599\uff09\u4e0eFe3<\/sub>O 4<\/sub>\u7eb3\u7c73\u9897\u7c92\u8fde\u63a5\u7684\u80bd\u63a5\u5934\u8fdb\u884c\u5207\u5272\uff0c\u4f7fANNA\u7684\u8367\u5149\u5f97\u5230\u6d3b\u5316\u3002\u901a\u8fc7\u6bd4\u8f83ANNA\u7684\u53ef\u53d8\u6fc0\u6d3b\u53d1\u5c04\u548cCy5.5\u7684\u6052\u5b9a\u8367\u5149\uff0c\u53ef\u4ee5\u5bf9MMP-9\u6d3b\u6027\u8fdb\u884c\u91cf\u5316\u5e76\u4f5c\u56fe\u3002\u5728\u8fd9\u79cd\u8bbe\u8ba1\u4e2d\uff0cFe3<\/sub>O 4<\/sub>\u9897\u7c92\u8f7d\u4f53\u4e0d\u4ec5\u4f5c\u4e3aANNA\u7684\u731d\u706d\u5242\uff0c\u800c\u4e14\u8fd8\u4f5c\u4e3a\u80bf\u7624\u7684\u78c1\u5171\u632f\u6210\u50cf\uff08MRI\uff09\u7684\u9020\u5f71\u5242\u3002<\/p>\n

\u56fe1. <\/strong>\u7eb3\u7c73\u63a2\u9488\u7684\u7cfb\u5217<\/strong>\u8868\u5f81\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/p>\n

\"\"a\uff09 \u7eb3\u7c73\u63a2\u9488\u7ed3\u6784\u793a\u610f\u56fe<\/p>\n

b\uff09 Fe3<\/sub>O 4<\/sub>\u7eb3\u7c73\u9897\u7c92\u7684TEM\u8868\u5f81<\/p>\n

c\uff09 Fe3<\/sub>O 4<\/sub>\u7eb3\u7c73\u9897\u7c92\u7684\u7684UV-vis-NIR\u5149\u8c31<\/p>\n

d\uff09 \u8d28\u5b50\u5316\/\u53bb\u8d28\u5b50\u5316\u8bf1\u5bfc\u7684ANNA\u7ed3\u6784\u8f6c\u53d8\uff1bCy5.5\u7684\u5316\u5b66\u7ed3\u6784\uff1b\u4e0d\u540cpH\u4e0bANNA\u548cCy5.5\u7684\u8367\u5149\u5149\u8c31\u56fe\u3002<\/p>\n

\u56fe2.<\/strong> \u7eb3\u7c73\u63a2\u9488\u7684\u4f53\u5916\u8367\u5149\u8868\u5f81<\/strong><\/p>\n

\"\"a\uff09 \u7eb3\u7c73\u63a2\u9488\u4e0e\u6fc0\u6d3b\u7684MMP-9\u4f5c\u7528\u4e0d\u540c\u65f6\u95f4\u540e\u5728455 nm\u548c650 nm\u6fc0\u53d1\u4e0b\u7684\u8367\u5149\u5149\u8c31<\/p>\n

b\uff09 ANNA(\u7eff\u8272)\u548cCy 5.5(\u7ea2\u8272)\u8367\u5149\u5cf0\u503c\u5f3a\u5ea6\u968f\u65f6\u95f4\u7684\u53d8\u5316<\/p>\n

c\uff09 \u7eb3\u7c73\u63a2\u9488\u4e0e\u6d53\u5ea6\u7684MMP-9\u5171\u5b75\u80b2\u540e\u7684\u8367\u5149\u5149\u8c31<\/p>\n

d\uff09 \u56fa\u5b9a\u5b75\u80b2\u65f6\u95f4\u4e3a30\u5206\u949f\uff0cANNA\u548cCy5.5\uff08IANNA \/ ICy5.5\uff09\u7684\u79ef\u5206\u53d1\u5c04\u5f3a\u5ea6\u6bd4\u7387\u4e0e\u6fc0\u6d3b\u7684MMP-9\u6d53\u5ea6\u4e4b\u95f4\u7684\u7ebf\u6027\u5173\u7cfb\uff08\u7ebf\u6027\u62df\u5408\u7ed3\u679c\u663e\u793a\u4f53\u5916MMP-9\u7684\u68c0\u6d4b\u6781\u9650\u4e3a0.74nM\uff0c\u7f6e\u4fe1\u6c34\u5e73\u4e3a99.9\uff05\uff09<\/p>\n

e\uff09 \u7eb3\u7c73\u63a2\u9488\u5b75\u80b26\u5c0f\u65f6\u540eLS180\u7ec6\u80de\uff08\u9876\u884c\uff09\u548c\u4eba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08\u5bf9\u7167\u7ec6\u80de\uff08\u5e95\u884c\uff09\uff09\u7684\u5171\u805a\u7126\u663e\u5fae\u955c\u56fe\u50cf\uff0c\u6839\u636e\u67d3\u6599\u7684\u4e0d\u540c\u53d1\u5c04\u6ce2\u957f\u7528\u4e0d\u540c\u7684\u901a\u9053\u6210\u50cf\uff08\u7ec6\u80de\u6838\uff1aHoechst\u67d3\u8272\uff0c\u6807\u5c3a\u4e3a10\u03bcm\uff09\u3002<\/p>\n

\u56fe3. \u8377\u7624\u5c0f\u9f20\u8367\u5149\u6210\u50cf\u548cMR\u6210\u50cf\u8868\u5f81<\/strong><\/p>\n

\"\"\u4e0a\u56fe\uff1a\u8377\u7624\u5c0f\u9f20\u76f8\u5e94\u660e\u573a\u56fe\u50cf\u4e0a\u7684\u80bf\u7624\u90e8\u4f4dCy5.5\u548cANNA\u4fe1\u53f7\u53d8\u5316\u7684\u8367\u5149\u56fe\u50cf\uff08\u53f3\u68461-9\uff1a\u5fc3\u810f\uff0c\u809d\u810f\uff0c\u813e\u810f\uff0c\u80ba\u810f\uff0c\u80be\u810f\uff0c\u80a0\u9053\uff0c\u9aa8\u9abc\uff0c\u808c\u8089\u548c\u80bf\u7624\u7b49\uff09\u3002<\/p>\n

\u4e0b\u56fe\uff1a\u9759\u8109\u6ce8\u5c04\u7eb3\u7c73\u63a2\u9488\u4e4b\u524d\u548c\u6ce8\u5c04\u540e\u4e0d\u540c\u65f6\u95f4\u70b9\u83b7\u5f97\u7684\u8377\u7624\u5c0f\u9f20\u7684T2\u52a0\u6743MR\u56fe\u50cf\u989c\u8272\u7f16\u7801\u56fe\uff08\u53f3\u6846\uff1a\u5177\u6709\u7c7b\u4f3c\u7ed3\u6784\u7684\u4e0d\u542bFA\u7684\u7eb3\u7c73\u63a2\u9488\u7684T2\u503c\u968f\u65f6\u95f4\u53d8\u5316\u4f5c\u4e3a\u5bf9\u7167\uff09\u3002<\/p>\n

\u56fe<\/strong>4. <\/strong>MMP-9<\/strong>\u548c<\/strong>pH<\/strong>\u503c\u7684\u4f53\u5185\u6210\u50cf\u56fe<\/strong><\/p>\n

\"\"a-b\uff09 MMP-9\u5728\u4f53\u5185\u6d3b\u6027\u5f3a\u5ea6\u7684\u7b49\u9ad8\u7ebf\u56fe\u548c\u80bf\u7624\u90e8\u4f4d\u7684pH\u503c\uff08\u767d\u8272\u865a\u7ebf\u8868\u793a\u7ec4\u7ec7\u5206\u6790\u5f97\u5230\u7684\u80bf\u7624\u6a2a\u622a\u9762\uff09<\/p>\n

c\uff09 \u4e09\u4e2a\u76f8\u90bb\u7ec4\u7ec7\u7684H&E\u5207\u7247\u4ee5\u53ca\u666e\u9c81\u58eb\u84dd\u67d3\u8272\u3002<\/p>\n

\u56fe<\/strong>5. <\/strong>MMP-9<\/strong>\u548c<\/strong>pH<\/strong>\u503c\u7684\u76f8\u4e92\u5173\u8054\u5206\u6790<\/strong><\/p>\n

\"\"a\uff09 \u7624\u5185\u6ce8\u5c04PBS\uff08pH 6.2\uff09\u8c03\u8282\u80bf\u7624pH\u540eMMP-9\u6d3b\u6027\u7684\u53d8\u5316<\/p>\n

b\uff09 \u901a\u8fc7\u6bd4\u8272\u6d4b\u5b9a\uff08\u5de6\uff09\u83b7\u5f97\u7684MMP-9\u7684\u5b9a\u91cf\u8868\u8fbe\u6c34\u5e73\u548c\u591a\u6a21\u6001\u6210\u50cf\uff08\u4e2d\uff09\u83b7\u5f97\u7684\u76f8\u5bf9MMP-9\u6d3b\u6027\uff0c\u4ee5\u53ca\u4f7f\u7528ANNA\u8367\u5149\u53d1\u5c04\uff08\u53f3\uff09\u91cf\u5316\u7684\u80bf\u7624\u5fae\u73af\u5883pH<\/p>\n

c\uff09 \u5728D + 0\uff0cD + 2\u548cD + 4\u83b7\u5f97\u7684\u80bf\u7624\u7684\u5b9a\u91cfpH\u548cMMP-9\u8868\u8fbe\u56fe\u8c31\uff08\u5728\u4ece\u9ed1\u8272\u5230\u9ec4\u8272\u7684\u989c\u8272\u6761\u9634\u5f71\u4e2d\uff0c\u7528\u4e8e\u8bfb\u53d6MMP-9\u8868\u8fbe\u6c34\u5e73\uff0c\u6bcf\u4e2a\u683c\u5bf9\u5e940.25\u4e2a\u5355\u4f4d\uff09<\/p>\n

d\uff09 4\u5929\u5185\u76ae\u4e0b\u80bf\u7624\u7684\u751f\u957f\u7167\u7247<\/p>\n

e\uff09 \u4e0a\u56fe\uff1aH\uff06E\u67d3\u8272\u7684\u7ec4\u7ec7\u8f7d\u73bb\u7247\u7684\u663e\u5fae\u56fe\u50cf; \u4e0b\u56fe\uff1a\u4e24\u4e2a\u76f8\u90bb\u8f7d\u73bb\u7247\u7528E-\u9499\u7c98\u86cb\u767d\u67d3\u8272\uff08\u7ea2\u8272\uff09\u548cMMP-9\u8868\u8fbe\uff08\u7eff\u8272\uff09\uff08\u6807\u5c3a\uff1a200 \u03bcm\uff09\u7684\u514d\u75ab\u8367\u5149\u663e\u5fae\u56fe\u50cf\u7684\u7ec4\u5408\u3002\u6a59\u8272\u7bad\u5934\u6307\u5411\u5065\u5eb7\u7ec4\u7ec7\u548cMMP-9\u9ad8\u5ea6\u8868\u8fbe\u7684\u80bf\u7624\u7ec4\u7ec7\u4e4b\u95f4\u7684\u5206\u754c\u7ebf\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

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\u00a0<\/strong>\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH in Vivo<\/strong><\/a>\uff08J. Am. Chem. Soc.\uff0c2017\uff0cDOI: 10.1021\/jacs.7b08900\uff09<\/p>\n

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