{"id":123665,"date":"2018-01-30T06:30:47","date_gmt":"2018-01-29T22:30:47","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=123665"},"modified":"2018-02-03T20:12:12","modified_gmt":"2018-02-03T12:12:12","slug":"%e8%8d%a7%e5%85%89%e7%94%9f%e7%89%a9%e6%8e%a2%e9%92%882017%e5%b9%b4%e7%a0%94%e7%a9%b6%e8%bf%9b%e5%b1%95%e6%a2%b3%e7%90%86","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=123665","title":{"rendered":"\u68b3\u7406\uff1a\u8fc7\u53bb\u4e00\u5e74\u8367\u5149\u751f\u7269\u63a2\u9488\u9886\u57df\u91cd\u5927\u7814\u7a76\u7a81\u7834"},"content":{"rendered":"

\u751f\u7269\u4f53\u5185\u7684\u4e00\u4e9b\u5316\u5b66\u7269\u8d28\u3001\u9176\u3001\u751f\u7269\u5927\u5206\u5b50\u7b49\u5bf9\u751f\u7269\u4f53\u7684\u751f\u547d\u6d3b\u52a8\u5747\u6709\u91cd\u8981\u7684\u5f71\u54cd\uff0c\u56e0\u6b64\uff0c\u57fa\u4e8e\u8fd9\u4e9b\u7269\u8d28\u7684\u68c0\u6d4b\u4e0e\u6210\u50cf\u5bf9\u4e8e\u79d1\u7814\u5de5\u4f5c\u8005\u63a2\u7d22\u751f\u547d\u79d1\u5b66\u7684\u672a\u77e5\u4e16\u754c\u6709\u7740\u91cd\u8981\u7684\u6307\u5bfc\u610f\u4e49\u3002\u76ee\u524d\uff0c\u9488\u5bf9\u751f\u7269\u4f53\u5185\u7684\u5316\u5b66\u4e0e\u751f\u7269\u7269\u8d28\u7684\u68c0\u6d4b\u4e0e\u6210\u50cf\uff0c\u79d1\u7814\u5de5\u4f5c\u8005\u5df2\u7ecf\u5f00\u53d1\u51fa\u4e86\u4e00\u7cfb\u5217\u7684\u65b9\u6cd5\uff0c\u6bd4\u5982\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u3001\u7535\u5b50\u81ea\u65cb\u5171\u632f\u3001\u6838\u78c1\u5171\u632f\u6210\u50cf\u7b49\u3002\u867d\u7136\u8fd9\u4e9b\u65b9\u6cd5\u4e5f\u80fd\u83b7\u5f97\u4e00\u4e9b\u7ed3\u679c\uff0c\u4f46\u662f\u7531\u4e8e\u4f9d\u8d56\u4e8e\u6602\u8d35\u7684\u4eea\u5668\u4ee5\u53ca\u8017\u65f6\u592a\u957f\uff0c\u4ed6\u4eec\u7684\u5e94\u7528\u53d7\u5230\u4e86\u4e00\u5b9a\u7684\u9650\u5236\u3002\u5bf9\u6bd4\u4e8e\u524d\u9762\u6240\u63d0\u5230\u7684\u8fd9\u4e9b\u68c0\u6d4b\u4e0e\u6210\u50cf\u7684\u65b9\u6cd5\uff0c\u57fa\u4e8e\u8367\u5149\u7684\u751f\u7269\u63a2\u9488\u56e0\u4e3a\u7075\u654f\u5ea6\u9ad8\u3001\u901f\u5ea6\u5feb\u3001\u65e0\u7834\u574f\u6027\u7b49\u4f18\u70b9\u53d7\u5230\u4e86\u5e7f\u6cdb\u7684\u5173\u6ce8\u3002\u57282017\u5e74\uff0c\u8367\u5149\u751f\u7269\u63a2\u9488\u5728\u68c0\u6d4b\u3001\u8bca\u7597\u548c\u6210\u50cf\u7b49\u9886\u57df\u90fd\u6709\u7740\u7cbe\u5f69\u7684\u8868\u73b0\u3002\u4e0b\u9762\u5c31\u7531\u6211\u5e26\u9886\u5927\u5bb6\u56de\u987e\u4e0e\u603b\u7ed3\u8367\u5149\u751f\u7269\u63a2\u94882017\u5e74\u7814\u7a76\u8fdb\u5c55\u3002<\/p>\n

1. \u57fa\u4e8e\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb<\/p>\n

\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u662f\u6307\u5728\u4e24\u4e2a\u4e0d\u540c\u7684\u8367\u5149\u57fa\u56e2\u4e2d\uff0c\u5982\u679c\u4e00\u4e2a\u8367\u5149\u57fa\u56e2\uff08\u4f9b\u4f53\uff09\u7684\u53d1\u5c04\u5149\u8c31\u4e0e\u53e6\u5916\u4e00\u4e2a\u57fa\u56e2\uff08\u53d7\u4f53\uff09\u7684\u5438\u6536\u5149\u8c31\u6709\u4e00\u5b9a\u7684\u91cd\u53e0\uff0c\u5f53\u8fd9\u4e24\u4e2a\u8367\u5149\u57fa\u56e2\u95f4\u7684\u8ddd\u79bb\u5c0f\u4e8e100\u57c3\u65f6\uff0c\u5c31\u53ef\u89c2\u5bdf\u5230\u8367\u5149\u80fd\u91cf\u7531\u4f9b\u4f53\u5411\u53d7\u4f53\u8f6c\u79fb\u7684\u73b0\u8c61\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u5728\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u53d1\u751f\u65f6\uff0c\u4ee5\u4f9b\u4f53\u7684\u6fc0\u53d1\u6ce2\u957f\u6fc0\u53d1\u65f6\uff0c\u53ef\u89c2\u5bdf\u5230\u53d7\u4f53\u53d1\u5c04\u7684\u8367\u5149\u3002<\/p>\n

\u67cf\u6797\u6d2a\u5821\u5927\u5b66\u7684Christoph Arenz\u6559\u6388\u5728Angew. Chem. Int. Ed.\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cAmplification of a FRET Probe by Lipid-Water Partition for the Detection of Acid Sphingomyelinase in Live Cells\u201d\u7684\u6587\u7ae0\u3002\u5728\u672c\u6587\u7684\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u3001\u5408\u6210\u4e86\u4e00\u79cd\u57fa\u4e8e\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u7684\u63a2\u9488\u3002\u8be5\u63a2\u9488\u7531\u4e00\u5bf9\u53ef\u4ee5\u53d1\u751f\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u7684\u8367\u5149\u56e2\u7ec4\u6210\uff0c\u4f9b\u4f537-methoxy-coumarin-3-carboxylate (MCC)\u548c\u53d7\u4f53nitrobenzoxadiazole (NBD)\uff0c\u5728\u7ed3\u6784\u4e0a\u5c5e\u4e8e\u9798\u78f7\u8102\u884d\u751f\u7269\uff0c\u56e0\u800c\u53ef\u4ee5\u88ab\u9178\u6027\u9798\u78f7\u8102\u9176\u7279\u5f02\u6027\u8bc6\u522b\u3002\u5f53\u8367\u5149\u57fa\u56e2MCC\u548cNBD\u76f8\u8ddd\u5f88\u8fd1\u65f6\uff0c\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u6548\u7387\u9ad8\uff0c\u53d1\u5149\u5728550nm\uff1b\u5f53\u9176\u5207\u53cd\u5e94\u53d1\u751f\u65f6\uff0c\u8367\u5149\u56e2\u4e4b\u95f4\u7684\u8ddd\u79bb\u53d8\u5927\uff0c\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u6548\u7387\u5927\u5927\u964d\u4f4e\uff0c550nm\u5904\u7684\u8367\u5149\u5f3a\u5ea6\u964d\u4f4e\u7684\u540c\u65f6\uff0c400nm\u5904\u7684\u8367\u5149\u5f3a\u5ea6\u589e\u52a0\uff0c\u4ece\u800c\u5b9e\u73b0\u4e86\u9178\u6027\u9798\u78f7\u8102\u9176\u7684\u7279\u5f02\u6027\u68c0\u6d4b\u3002<\/p>\n

\"\"<\/p>\n

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\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201611706\/epdf<\/a><\/p>\n

2. \u5149\u81f4\u7535\u5b50\u8f6c\u79fb<\/p>\n

\u5149\u81f4\u7535\u5b50\u8f6c\u79fb\u4f53\u7cfb\u4e00\u822c\u662f\u901a\u8fc7\u95f4\u9694\u57fa\u56e2\u5c06\u7535\u5b50\u7ed9\u4f53\u548c\u7535\u5b50\u53d7\u4f53\u76f8\u8fde\u6784\u6210\u3002\u5728\u6fc0\u53d1\u5149\u7167\u5c04\u4e0b\uff0c\u7535\u5b50\u7ed9\u4f53\u7684\u7535\u5b50\u4ece\u57fa\u6001\u6fc0\u53d1\u5230\u6fc0\u53d1\u6001\u3002\u7531\u4e8e\u7535\u5b50\u7ed9\u53d7\u4f53\u95f4\u7684\u7535\u5b50\u8f6c\u79fb\u4f5c\u7528\uff0c\u7ed9\u4f53\u6fc0\u53d1\u6001\u7684\u7535\u5b50\u8f6c\u79fb\u5230\u7535\u5b50\u53d7\u4f53\uff0c\u800c\u65e0\u6cd5\u56de\u5230\u57fa\u6001\uff0c\u4ece\u800c\u5bfc\u81f4\u8367\u5149\u6dec\u706d\u3002<\/p>\n

\u6765\u81ea\u534e\u5357\u7406\u5de5\u5927\u5b66\u7684\u5434\u6c34\u73e0\u6559\u6388\u56e2\u961f\u4e8e2017\u5e747\u6708\u5728Biomaterials\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cA self-immolative prodrug nanosystem capable of releasing a drug and a NIR reporter for in vivo imaging and therapy\u201d\u3002\u8fd9\u4e2a\u5de5\u4f5c\u8bbe\u8ba1\u5408\u6210\u4e86\u4e00\u79cd\u201c\u8bca\u7597\u5408\u4e00\u201d\u7684\u751f\u7269\u63a2\u9488\uff0c\u5c06\u6297\u764c\u836f\u7269\u559c\u6811\u78b1\u3001\u8fd1\u7ea2\u5916\u8367\u5149\u67d3\u6599\u548c\u5bf9\u8c37\u80f1\u7518\u80bd\u7279\u5f02\u6027\u54cd\u5e94\u7684\u4e8c\u785d\u57fa\u82ef\u78fa\u9170\u57fa\u56e2\uff0c\u901a\u8fc7\u4e00\u4e2a\u5177\u6709self-immolative\u7279\u6027\u7684\u95f4\u9694\u57fa\u56e2\u8fde\u63a5\u8d77\u6765\u3002\u4e8c\u785d\u57fa\u82ef\u78fa\u9170\u57fa\u7531\u4e8e\u5f3a\u5438\u7535\u5b50\u4f5c\u7528\uff0c\u548c\u8367\u5149\u56e2\u4e4b\u95f4\u53d1\u751f\u5149\u81f4\u7535\u5b50\u8f6c\u79fb\uff0c\u8fd1\u7ea2\u5916\u8367\u5149\u67d3\u6599\u7684\u8367\u5149\u56e0\u6b64\u53d1\u751f\u6dec\u706d\u3002\u5f53\u4e8c\u785d\u57fa\u82ef\u78fa\u9170\u57fa\u56e2\u4e0e\u8c37\u80f1\u7518\u80bd\u53d1\u751f\u4f5c\u7528\u88ab\u5207\u9664\uff0c\u03b2-\u6d88\u9664\u7684\u53cd\u5e94\u53d1\u751f\uff0c\u559c\u6811\u78b1\u548c\u8367\u5149\u56e2\u540c\u65f6\u91ca\u653e\u51fa\u6765\uff0c\u5149\u81f4\u7535\u5b50\u8f6c\u79fb\u4f5c\u7528\u6d88\u5931\uff0c\u8367\u5149\u5f3a\u5ea6\u9010\u6e10\u589e\u5f3a\u3002\u56e0\u800c\u53ef\u4ee5\u5b9e\u73b0\u57fa\u4e8e\u764c\u75c7\u7ec6\u80de\u4e2d\u57fa\u4e8e\u8c37\u80f1\u7518\u80bd\u54cd\u5e94\u7684\u764c\u75c7\u8bca\u65ad\u548c\u6cbb\u7597\u3002\u8fd9\u4e2a\u63a2\u9488\u5728\u7ec6\u80de\u548c\u5c0f\u9f20\u4e0a\u5747\u53d6\u5f97\u4e86\u5f88\u597d\u7684\u6548\u679c\u3002<\/p>\n

\"\"<\/p>\n

\u57fa\u4e8e\u5149\u81f4\u7535\u5b50\u8f6c\u79fb\u7684\u751f\u7269\u8bca\u7597\u63a2\u9488<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttps:\/\/ac.els-cdn.com\/S0142961217303952\/1-s2.0-S0142961217303952-main.pdf?_tid=97736f76-0372-11e8-986d-00000aab0f6b&acdnat=1517065314_7bf160badddb0a6361be395fbe1d0027<\/a><\/p>\n

3. \u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb<\/p>\n

\u53d1\u751f\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u7684\u4f53\u7cfb\u4e00\u822c\u662f\u63a8\u7535\u5b50\u57fa\u56e2\u548c\u5438\u7535\u5b50\u57fa\u56e2\u901a\u8fc7\u03c0\u7535\u5b50\u5171\u8f6d\u4f53\u7cfb\u8fde\u63a5\u800c\u6210\uff0c\u5728\u57fa\u6001\u65f6\uff0c\u8868\u73b0\u4e3a\u6781\u5316\u7ed3\u6784\uff1b\u800c\u5728\u5149\u6fc0\u53d1\u4e0b\uff0c\u5076\u6781\u77e9\u589e\u5927\uff0c\u5f3a\u5316\u4e86\u8fd9\u79cd\u7279\u6027\u3002\u5f53\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u4f5c\u7528\u589e\u5f3a\uff0c\u5206\u5b50\u7684\u53d1\u5149\u7ea2\u79fb\uff1b\u5f53\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u4f5c\u7528\u51cf\u5f31\uff0c\u5206\u5b50\u7684\u53d1\u5149\u84dd\u79fb\u3002<\/p>\n

\u5510\u672c\u5fe0\u9662\u58eb\u8bfe\u9898\u7ec4\u4e8e2017\u5e741\u6708\u5728Chem. Commun.\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cA two-channel responsive fluorescent probe with AIE characteristics and its application for selective imaging of superoxide anions in living cells\u201d\u7684\u6587\u7ae0\u3002\u6d3b\u6027\u6c27\u4f5c\u4e3a\u751f\u7269\u4f53\u5185\u7684\u4e00\u79cd\u91cd\u8981\u7269\u8d28\uff0c\u4e0e\u7ec6\u80de\u51cb\u4ea1\u3001\u764c\u75c7\u3001\u708e\u75c7\u7b49\u75be\u75c5\u90fd\u6709\u91cd\u5927\u7684\u8054\u7cfb\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u57fa\u4e8e\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u7684\u751f\u7269\u8367\u5149\u63a2\u9488\uff0c\u5b9e\u73b0\u4e86\u9488\u5bf9\u8d85\u6c27\u9634\u79bb\u5b50\u7684\u53cc\u901a\u9053\u68c0\u6d4b\u4e0e\u6210\u50cf\u3002\u63a2\u9488\u7684\u7ed3\u6784\u4e3a\u63a8\u7535\u5b50\u7684\u7532\u6c27\u57fa\u548c\u62c9\u7535\u5b50\u5421\u5576\u76d0\u901a\u8fc7\u56db\u82ef\u57fa\u4e59\u70ef\u5171\u8f6d\u76f8\u8fde\u3002\u7531\u4e8e\u5421\u5576\u76d0\u7684\u62c9\u7535\u5b50\u4f5c\u7528\u8f83\u5f3a\uff0c\u63a2\u9488\u7684\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u4f5c\u7528\u8f83\u5f3a\uff0c\u4f7f\u5f97\u5206\u5b50\u7684\u53d1\u5149\u5728600nm\u5de6\u53f3\uff1b\u5f53\u63a2\u9488\u4e0e\u8d85\u6c27\u9634\u79bb\u5b50\u4f5c\u7528\u540e\uff0c\u5421\u5576\u76d0\u90e8\u5206\u53d8\u6210\u5421\u5576\uff0c\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u4f5c\u7528\u53d8\u5f31\uff0c\u63a2\u9488\u7684\u53d1\u5149\u84dd\u79fb\u5230520nm\uff0c\u4ece\u800c\u5b9e\u73b0\u4e86\u5bf9\u8d85\u6c27\u9634\u79bb\u5b50\u7684\u53cc\u901a\u9053\u68c0\u6d4b\u4e0e\u6210\u50cf\u3002\u63a2\u9488\u5206\u5b50\u5728\u7ec6\u80de\u708e\u75c7\u548c\u51cb\u4ea1\u6a21\u578b\u4e2d\u5747\u6709\u5f88\u597d\u7684\u6548\u679c\u3002<\/p>\n

\"\"<\/p>\n

\u57fa\u4e8e\u5206\u5b50\u5185\u7535\u8377\u8f6c\u79fb\u7684\u53cc\u901a\u9053\u8367\u5149\u63a2\u9488<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/pubs.rsc.org\/-\/content\/articlehtml\/2017\/cc\/c6cc09307h<\/a><\/p>\n

4. \u5206\u5b50\u4f59\u8f89\u6210\u50cf<\/p>\n

\u4f20\u7edf\u8367\u5149\u6210\u50cf\u7531\u4e8e\u6fc0\u53d1\u5149\u5728\u751f\u7269\u4f53\u5185\u4f1a\u4ea7\u751f\u5e72\u6270\u6027\u5f88\u5f3a\u7684\u81ea\u8367\u5149\uff0c\u4ece\u800c\u5927\u5927\u964d\u4f4e\u6210\u50cf\u7684\u7075\u654f\u5ea6\u548c\u4fe1\u566a\u6bd4\u3002\u4f59\u8f89\u53d1\u5149\u662f\u6307\u5149\u6fc0\u53d1\u540e\uff0c\u6750\u6599\u4f1a\u6709\u6301\u7eed\u53d1\u5149\u7684\u8fc7\u7a0b\u3002\u5176\u673a\u7406\u4e3a\u7ecf\u8fc7\u70ed\u6fc0\u6d3b\u7684\u6750\u6599\uff0c\u5149\u5b50\u4f1a\u4ece\u80fd\u9631\u4e2d\u7f13\u6162\u91ca\u653e\u3002\u548c\u5bff\u547d\u5728ns\u7ea7\u522b\u7684\u80cc\u666f\u8367\u5149\u76f8\u6bd4\uff0c\u4f59\u8f89\u53d1\u5149\u53ef\u4ee5\u6301\u7eed\u66f4\u4e45\uff0c\u5728\u80cc\u666f\u8367\u5149\u6d88\u5931\u540e\u518d\u6536\u96c6\u4f59\u8f89\u53d1\u5149\uff0c\u53ef\u4ee5\u6709\u6548\u5730\u6d88\u9664\u80cc\u666f\u8367\u5149\u7684\u5e72\u6270\uff0c\u63d0\u9ad8\u6210\u50cf\u8d28\u91cf\u3002<\/p>\n

\u6765\u81ea\u65b0\u52a0\u5761\u5357\u6d0b\u7406\u5de5\u5927\u5b66\u7684\u6d66\u4f83\u88d4\u6559\u6388\u8bfe\u9898\u7ec4\u5728Nature Biotechnology\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cMolecular afterglow imaging with bright, biodegradable polymer nanoparticles\u201d\u7684\u6587\u7ae0\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u4e00\u79cd\u534a\u8870\u671f\u957f\u8fbe6\u5206\u949f\u3001\u8fd1\u7ea2\u5916\u53d1\u5149\u7684\u5171\u8f6d\u9ad8\u5206\u5b50\u3002\u4e0e\u4f20\u7edf\u65e0\u673a\u4f59\u8f89\u53d1\u5149\u63a2\u9488\u76f8\u6bd4\uff0c\u8fd9\u79cd\u5171\u8f6d\u9ad8\u5206\u5b50\u6750\u6599\u5177\u6709\u66f4\u5f3a\u7684\u4eae\u5ea6\uff08100\u500d\u4ee5\u4e0a\uff09\u548c\u66f4\u6df1\u7684\u7ec4\u7ec7\u7a7f\u900f\u6df1\u5ea6\uff0c\u540c\u65f6\u5177\u6709\u66f4\u4f4e\u7684\u6bd2\u6027\uff1b\u4e0e\u4f20\u7edf\u7684\u8fd1\u7ea2\u5916\u6750\u6599\u76f8\u6bd4\uff0c\u8fd9\u79cd\u5171\u8f6d\u9ad8\u5206\u5b50\u6750\u6599\u5728\u5c0f\u9f20\u6d3b\u4f53\u6dcb\u5df4\u7ed3\u548c\u80bf\u7624\u6210\u50cf\u4e2d\uff0c\u5177\u6709\u66f4\u9ad8\u7684\u4fe1\u53f7\u5f3a\u5ea6\uff08127\u500d\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u4f59\u8f89\u53d1\u5149\u7684\u8367\u5149\u63a2\u9488\u5728\u6210\u50cf\u4e0a\u7684\u5e94\u7528<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1awww.nature.com\/articles\/nbt.3987.pdf<\/a><\/p>\n

5. \u5316\u5b66\u53d1\u5149<\/p>\n

\u5316\u5b66\u53d1\u5149\u662f\u6307\u7269\u8d28\u5728\u8fdb\u884c\u5316\u5b66\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u4f34\u968f\u7684\u4e00\u79cd\u5149\u8f90\u5c04\u73b0\u8c61\u3002A\u4e0eB\u4e24\u79cd\u7269\u8d28\u5728\u53d1\u751f\u5316\u5b66\u53cd\u5e94\u91ca\u653e\u80fd\u91cf\uff0c\u91ca\u653e\u7684\u80fd\u91cf\u88ab\u7269\u8d28C\u5438\u6536\u3002\u7269\u8d28C\u5438\u6536\u80fd\u91cf\u540e\uff0c\u4ece\u57fa\u6001\u8dc3\u8fc1\u81f3\u6fc0\u53d1\u6001C*\uff0c\u53c8\u56de\u5230\u57fa\u6001\u7684\u8fc7\u7a0b\u4e2d\uff0c\u4ea7\u751f\u5149\u8f90\u5c04\u3002<\/p>\n

\u6765\u81ea\u4ee5\u8272\u5217\u7279\u62c9\u7ef4\u592b\u5927\u5b66\u7684Doron Shabat\u8bfe\u9898\u7ec4\u4e8e2017\u5e747\u6708\u5728J. Am. Chem. Soc.\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cSelf-immolative chemiluminescence polymers: innate assimilation of chemiexcitation in a domino-like depolymerization\u201d\u7684\u6587\u7ae0\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u4e00\u79cd\u5177\u6709self-immolative\u6027\u8d28\u7684\u805a\u5408\u7269\u3002\u8fd9\u79cd\u805a\u5408\u7269\u5728\u4e00\u5b9a\u7684\u5916\u754c\u523a\u6fc0\u4e0b\uff0c\u4f1a\u53d1\u751f\u591a\u7c73\u8bfa\u4f3c\u7684\u94fe\u5f0f\u8fde\u9501\u53cd\u5e94\uff0c\u5728\u918c-\u7532\u57fa\u6d88\u9664\u53cd\u5e94\u53d1\u751f\u7684\u540c\u65f6\uff0c\u53d1\u751f\u5316\u5b66\u53d1\u5149\uff0c\u5b9e\u73b0\u5bf9\u5916\u754c\u523a\u6fc0\u73af\u5883\u7684\u7279\u5f02\u6027\u54cd\u5e94\u3002\u56e0\u6b64\u901a\u8fc7\u6539\u53d8\u6b64\u805a\u5408\u7269\u7684\u5f00\u5173\u7ed3\u6784\uff0c\u53ef\u4ee5\u5b9e\u73b0\u7279\u5f02\u6027\u5730\u68c0\u6d4b\u591a\u79cd\u7269\u8d28\u3002\u8fd9\u4e2a\u5de5\u4f5c\u4e3a\u4fe1\u53f7\u653e\u5927\u3001\u5149\u7167\u660e\u6750\u6599\u3001\u5206\u5b50\u63a2\u9488\u548c\u6210\u50cf\u7b49\u76f8\u5173\u9886\u57df\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\uff0c\u5177\u6709\u4e00\u5b9a\u7684\u542f\u53d1\u610f\u4e49\u3002<\/p>\n

\"\"<\/p>\n

Self-immolative\u805a\u5408\u7269\u7684\u7ed3\u6784\u53ca\u5176\u5316\u5b66\u53d1\u5149\u673a\u7406<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.7b04804<\/a><\/p>\n

6. \u591a\u5149\u5b50\u6210\u50cf<\/p>\n

\u8367\u5149\u6210\u50cf\u7684\u9ad8\u901f\u53d1\u5c55\u8981\u6c42\u6750\u6599\u5177\u6709\u66f4\u597d\u7684\u7ec4\u7ec7\u7a7f\u900f\u80fd\u529b\u3001\u5bf9\u751f\u7269\u4f53\u7684\u4f24\u5bb3\u66f4\u4f4e\uff0c\u66f4\u9ad8\u7684\u4fe1\u566a\u6bd4\u548c\u51fa\u4f17\u7684\u65f6\u7a7a\u5206\u8fa8\u7387\u3002\u7136\u800c\uff0c\u5e38\u89c4\u7684\u5355\u5149\u5b50\u8367\u5149\u6750\u6599\u5e76\u4e0d\u80fd\u6ee1\u8db3\u8fd9\u4e9b\u9700\u6c42\u3002\u5355\u5149\u5b50\u8367\u5149\u6750\u6599\u7684\u6fc0\u53d1\u5149\u6ce2\u957f\u901a\u5e38\u5728350-500\u7eb3\u7c73\u3002\u8fd9\u79cd\u6ce2\u957f\u7684\u6fc0\u53d1\u5149\u6ce2\u957f\u8f83\u77ed\u4e14\u80fd\u91cf\u8f83\u9ad8\uff0c\u56e0\u800c\u5176\u7ec4\u7ec7\u7a7f\u900f\u6df1\u5ea6\u901a\u5e38\u5c0f\u4e8e100\u5fae\u7c73\uff0c\u4e14\u4f1a\u5bf9\u751f\u7269\u4f53\u9020\u6210\u4e00\u5b9a\u7684\u4f24\u5bb3\u3002\u4e0d\u4ec5\u5982\u6b64\uff0c\u8fd9\u4e2a\u6ce2\u957f\u7684\u6fc0\u53d1\u5149\u53ef\u4ee5\u6fc0\u53d1\u751f\u7269\u4f53\u7684\u81ea\u8367\u5149\uff0c\u5bf9\u6210\u50cf\u4fe1\u53f7\u9020\u6210\u5e72\u6270\uff0c\u964d\u4f4e\u4fe1\u566a\u6bd4\u3002\u591a\u5149\u5b50\u6210\u50cf\u662f\u6307\u6750\u6599\u540c\u65f6\u5438\u6536\u591a\u4e2a\u5149\u5b50\uff0c\u4ece\u57fa\u6001\u8dc3\u8fc1\u5230\u6fc0\u53d1\u6001\u7684\u975e\u7ebf\u6027\u5149\u5b66\u8fc7\u7a0b\u3002\u4e0e\u5355\u5149\u5b50\u6210\u50cf\u76f8\u6bd4\uff0c\u53cc\/\u4e09\u5149\u5b50\u6210\u50cf\u5728\u7ec4\u7ec7\u7a7f\u900f\u80fd\u529b\u3001\u751f\u7269\u4f53\u4fdd\u62a4\u3001\u4fe1\u566a\u6bd4\u548c\u65f6\u7a7a\u5206\u8fa8\u7387\u4e0a\u5747\u6709\u5f88\u597d\u7684\u8868\u73b0\u3002\u56e0\u6b64\uff0c\u53cc\/\u4e09\u5149\u5b50\u6210\u50cf\u5728\u6210\u50cf\u9886\u57df\u53d7\u5230\u4e86\u5e7f\u6cdb\u7684\u5173\u6ce8\u3002<\/p>\n

\u6765\u81ea\u6d59\u6c5f\u5927\u5b66\u7684\u94b1\u9a8f\u6559\u6388\u8bfe\u9898\u7ec4\u4e8e2017\u5e7410\u6708\u5728ACS Nano\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cAggregation-Induced Emission Luminogen with Deep-Red Emission for Through-Skull Three-Photon Fluorescence Imaging of Mouse\u201d\u7684\u6587\u7ae0\u3002\u9885\u5185\u6210\u50cf\u5728\u795e\u7ecf\u79d1\u5b66\u548c\u4e34\u5e8a\u533b\u5b66\u9886\u57df\u90fd\u6709\u5f88\u91cd\u8981\u7684\u610f\u4e49\uff0c\u4f20\u7edf\u7684X\u5c04\u7ebf\u8ba1\u7b97\u673a\u65ad\u5c42\u6210\u50cf\u548c\u78c1\u5171\u632f\u6210\u50cf\u5b58\u5728\u7a7a\u95f4\u5206\u8fa8\u7387\u8f83\u4f4e\u7684\u7f3a\u9677\u3002\u8fd9\u4e2a\u5de5\u4f5c\u5229\u7528\u7ea2\u5149AIE\u6750\u6599\uff0c\u907f\u514d\u4e86\u53cc\u5149\u5b50\u6210\u50cf\u6240\u9700\u8981\u7684\u5f00\u9885\u624b\u672f\u548c\u8111\u9885\u5e8a\u6280\u672f\uff0c\u5b9e\u73b0\u4e86\u5168\u9885\u6210\u50cf\u3002\u5934\u76d6\u9aa8\u7a7f\u900f\u6df1\u5ea6\u8fbe\u5230\u4e86300\u5fae\u7c73\uff0c2.4\u5fae\u7c73\u7684\u5c0f\u8840\u7ba1\u4e5f\u4f9d\u7136\u4e5f\u88ab\u89c2\u5bdf\u5230\u3002<\/p>\n

\"\"<\/p>\n

\u9885\u5185\u4e09\u5149\u5b50\u6210\u50cf<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.7b05645<\/a><\/p>\n

7. \u805a\u96c6\u8bf1\u5bfc\u53d1\u5149<\/p>\n

\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u662f\u6307\u4e00\u7c7b\u5177\u6709\u87ba\u65cb\u72b6\u7ed3\u6784\u7684\u5206\u5b50\u5728\u5206\u6563\u7684\u6eb6\u6db2\u6001\u4e0d\u53d1\u5149\uff0c\u800c\u5728\u5206\u5b50\u805a\u96c6\u7684\u72b6\u6001\u4e0b\u8367\u5149\u663e\u8457\u589e\u5f3a\u7684\u73b0\u8c61\u3002\u5f53\u8fd9\u4e9b\u5206\u5b50\u4ee5\u5355\u5206\u5b50\u5f62\u5f0f\u5b58\u5728\u4e8e\u6eb6\u6db2\u4e2d\u65f6\uff0c\u6fc0\u53d1\u6001\u7684\u7535\u5b50\u4ee5\u5206\u5b50\u5185\u8fd0\u52a8\uff08\u65cb\u8f6c\u548c\u632f\u52a8\uff09\u7684\u80fd\u91cf\u5f1b\u8c6b\u56de\u5230\u57fa\u6001\uff0c\u6b64\u65f6\u5206\u5b50\u4e0d\u53d1\u5149\uff1b\u800c\u5728\u5206\u5b50\u5904\u4e8e\u805a\u96c6\u6001\u65f6\uff0c\u5206\u5b50\u7684\u65cb\u8f6c\u548c\u632f\u52a8\u53d7\u5230\u9650\u5236\uff0c\u6fc0\u53d1\u6001\u7684\u7535\u5b50\u53ea\u80fd\u901a\u8fc7\u8f90\u5c04\u8dc3\u8fc1\u7684\u65b9\u5f0f\u56de\u5230\u57fa\u6001\uff0c\u56e0\u800c\u89c2\u5bdf\u5230\u8367\u5149\u589e\u5f3a\u7684\u73b0\u8c61\u3002\u56e0\u6b64\u4e00\u90e8\u5206\u57fa\u4e8e\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u7684\u63a2\u9488\u662f\u901a\u8fc7\u63a2\u9488\u6eb6\u89e3\u6027\u6539\u53d8\u5b9e\u73b0\u7684\u3002\u548c\u4f20\u7edf\u8367\u5149\u63a2\u9488\u76f8\u6bd4\uff0c\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u63a2\u9488\u5177\u6709\u6210\u50cf\u80cc\u666f\u4f4e\uff0c\u4fe1\u566a\u6bd4\u9ad8\u7684\u7279\u70b9\u3002<\/p>\n

\u6765\u81ea\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u7684\u5218\u658c\u6559\u6388\u8bfe\u9898\u7ec4\u4e8e2017\u5e741\u6708\u5728Chem. Sci.\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cLight-up probe based on AIEgens: dual signal turnon for caspase cascade activation monitoring\u201d\u7684\u6587\u7ae0\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u4e00\u79cd\u53ef\u4ee5\u540c\u65f6\u68c0\u6d4bcaspase-3\u548ccaspase-8\u7684\u8367\u5149\u63a2\u9488\u3002\u8be5\u63a2\u9488\u7531\u4e00\u6bb5\u53ef\u88abcaspase-3\u548ccaspase-8\u8bc6\u522b\u7684\u6c34\u6eb6\u6027\u591a\u80bd\uff0c\u5c06\u5206\u522b\u53d1\u84dd\u8272\u8367\u5149\u548c\u7ea2\u8272\u8367\u5149\u7684AIE\u8367\u5149\u56e2\u8fde\u63a5\u8d77\u6765\u3002\u63a2\u9488\u5206\u5b50\u7531\u4e8e\u5f88\u597d\u7684\u6c34\u6eb6\u6027\uff0c\u5728\u6eb6\u6db2\u4e2d\u4e0d\u53d1\u5149\u3002caspase-8\u53ef\u4ee5\u5c06\u591a\u80bd\u5207\u65ad\uff0c\u91ca\u653e\u51fa\u4e0d\u6c34\u6eb6\u7684\u84dd\u5149\u8367\u5149\u56e2\uff1b\u800ccaspase-3\u53ef\u4ee5\u5c06\u591a\u80bd\u5207\u65ad\uff0c\u91ca\u653e\u51fa\u4e0d\u6c34\u6eb6\u7684\u7ea2\u5149\u8367\u5149\u56e2\uff0c\u56e0\u6b64\u5b9e\u73b0\u9488\u5bf9\u4e24\u79cd\u9176\u6d3b\u6027\u7684\u8367\u5149\u68c0\u6d4b\u3002\u63a2\u9488\u5728\u6eb6\u6db2\u6001\u68c0\u6d4b\u548c\u7ec6\u80de\u5185\u6210\u50cf\u5747\u53d6\u5f97\u4e86\u5f88\u597d\u7684\u6548\u679c\u3002<\/p>\n

\"\"<\/p>\n

\u57fa\u4e8e\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u7684\u63a2\u9488\u5728caspase-3\u548ccaspase-8\u68c0\u6d4b\u4e2d\u7684\u5e94\u7528<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/pubs.rsc.org\/-\/content\/articlehtml\/2017\/sc\/c6sc04322d<\/a><\/p>\n

8. \u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb<\/p>\n

\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u662f\u6307\u5206\u5b50\u53d7\u5149\u6fc0\u53d1\u5230\u6fc0\u53d1\u6001\u540e\uff0c\u53d1\u751f\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u90e8\u76f8\u8fd1\u7684\u8d28\u5b50\u7ed9\u4f53\u4e0e\u8d28\u5b50\u53d7\u4f53\u95f4\u7684\u8d28\u5b50\u8f6c\u79fb\u73b0\u8c61\u3002\u901a\u5e38\u4e3a\u8d28\u5b50\u4ece\u7ed9\u4f53\u6cbf\u7740\u5206\u5b50\u6c22\u952e\u5230\u8fbe\u76f8\u90bb\u7684N\u3001O\u3001S\u7b49\u6742\u539f\u5b50\u4e0a\u7684\u5f02\u6784\u5316\u8fc7\u7a0b\u3002\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u4f5c\u4e3a\u5149\u5316\u5b66\u548c\u5149\u7269\u7406\u4e2d\u7684\u91cd\u8981\u53cd\u5e94\u53d7\u5230\u4e86\u5e7f\u6cdb\u7684\u5173\u6ce8\u548c\u7814\u7a76\u3002\u76ee\u524d\uff0c\u57fa\u4e8e\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u800c\u8bbe\u8ba1\u7684\u8367\u5149\u63a2\u9488\u5df2\u6709\u5f88\u591a\u3002<\/p>\n

\u6765\u81ea\u4e0a\u6d77\u534e\u4e1c\u7406\u5de5\u5927\u5b66\u7684\u94b1\u65ed\u7ea2\u9662\u58eb\u8bfe\u9898\u7ec4\u4e8e2017\u5e741\u6708\u5728Dyes and Pigments\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cA novel \u2018\u2018donor-two-acceptor\u2019\u2019 type fluorophore-based probe for fast detection and intracellular imaging of nitroreductase\u201d\u7684\u6587\u7ae0\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u5408\u6210\u4e86\u4e00\u79cd\u57fa\u4e8e\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u673a\u7406\u3001\u53ef\u68c0\u6d4b\u785d\u57fa\u8fd8\u539f\u9176\u6d3b\u6027\u7684\u8367\u5149\u63a2\u9488\u3002\u5f53\u785d\u57fa\u88ab\u785d\u57fa\u8fd8\u539f\u9176\u8fd8\u539f\u6210\u6c28\u57fa\u540e\uff0c\u7f9f\u57fa\u8131\u4fdd\u62a4\uff0c\u5e76\u4e0e\u82ef\u5e76\u567b\u5511\u4e0a\u7684\u6c2e\u539f\u5b50\u4e4b\u95f4\u5f62\u6210\u6c22\u952e\u3002\u6b64\u65f6\uff0c\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u8fc7\u7a0b\u53d1\u751f\uff0c\u5728\u7ea2\u5149\u6ce2\u6bb5\u7684\u8367\u5149\u5f3a\u5ea6\u589e\u52a0\uff0c\u5b9e\u73b0\u9488\u5bf9\u785d\u57fa\u8fd8\u539f\u9176\u7684\u68c0\u6d4b\u3002\u8fd9\u4e2a\u63a2\u9488\u53ef\u7528\u6765\u68c0\u6d4b\u7ec6\u80de\u5185\u7f3a\u6c27\u73af\u5883\u3002<\/p>\n

\"\"<\/p>\n

\u57fa\u4e8e\u6fc0\u53d1\u6001\u5206\u5b50\u5185\u8d28\u5b50\u8f6c\u79fb\u7684\u785d\u57fa\u8fd8\u539f\u9176\u6d3b\u6027\u68c0\u6d4b<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttps:\/\/www.sciencedirect.com\/science\/article\/pii\/S0143720816304119<\/a><\/p>\n

9. \u751f\u7269\u6b63\u4ea4\u5316\u5b66<\/p>\n

\u751f\u7269\u6b63\u4ea4\u5316\u5b66\u6307\u7684\u662f\u5728\u751f\u547d\u7cfb\u7edf\u5185\u80fd\u591f\u53d1\u751f\u7684\uff0c\u5e76\u4e14\u4e0d\u5f71\u54cd\u751f\u7269\u4f53\u672c\u8eab\u5316\u5b66\u8fc7\u7a0b\u7684\u5316\u5b66\u53cd\u5e94\u3002\u4e00\u4e2a\u5178\u578b\u7684\u751f\u7269\u6b63\u4ea4\u5316\u5b66\u5b9e\u9a8c\u4e3b\u8981\u5206\u4e3a\u4e24\u6b65\u3002\u7b2c\u4e00\u6b65\u751f\u7269\u6807\u8bb0\u662f\uff0c\u4e00\u4e2a\u7b80\u5355\u7684\u5316\u5b66\u57fa\u56e2\uff08\u6bd4\u5982\u78f7\u9178\u57fa\u56e2\u3001\u53e0\u6c2e\u57fa\u56e2\u7b49\uff09\uff0c\u5229\u7528\u751f\u7269\u4f53\u7cfb\u7edf\uff08\u7ec6\u80de\u6216\u5b8c\u6574\u7684\u751f\u7269\u4f53\uff09\u81ea\u8eab\u7684\u751f\u7269\u5408\u6210\u65b9\u5f0f\u6574\u5408\u5230\u76ee\u6807\u751f\u7269\u5206\u5b50\u4e0a\u7684\u8fc7\u7a0b\uff1b\u7b2c\u4e8c\u6b65\u5316\u5b66\u6807\u8bb0\u4e3a\uff0c\u5229\u7528\u80fd\u4e0e\u7b2c\u4e00\u6b65\u4e2d\u7684\u5c0f\u5206\u5b50\u7279\u5f02\u6027\u7ed3\u5408\u7684\u5316\u5b66\u53d1\u5149\u57fa\u56e2\u5728\u4e00\u5b9a\u7684\u6761\u4ef6\u4e0b\u4e0e\u5c0f\u5206\u5b50\u7ed3\u5408\u6216\u53cd\u5e94\uff0c\u4ece\u800c\u53ef\u4ee5\u62a5\u544a\u4f53\u5185\u88ab\u6807\u8bb0\u7684\u5927\u5206\u5b50\u7684\u5b9e\u65f6\u72b6\u6001\u3002<\/p>\n

\u6765\u81ea\u7ebd\u7ea6\u5dde\u7acb\u5927\u5b66\u7684qing lin\u6559\u6388\u4e8e2017\u5e749\u6708\u5728J. Am. Chem. Soc.\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201cSpirohexene-Tetrazine Ligation Enables Bioorthogonal Labeling of Class B G Protein-Coupled Receptors in Live Cells\u201d\u7684\u6587\u7ae0\u3002\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u5229\u7528\u57fa\u56e0\u7f16\u7801\u6269\u5c55\u6280\u672f\uff0c\u5c06\u8d56\u6c28\u9178\u7684spirohexene\u884d\u751f\u7269\u63d2\u5165\u5230\u4e86B\u578bG\u86cb\u767d\u5076\u8054\u53d7\u4f53\uff08GPCR\uff09\u4e2d\u53bb\u3002\u518d\u5229\u7528spirohexene\u4e0etetrazine\u4e4b\u95f4\u7684Diels-Alder\u53cd\u5e94\uff0c\u5b9e\u73b0\u5bf9GPCR\u7684\u8367\u5149\u6210\u50cf\uff0c\u5728\u6eb6\u6db2\u6001\u548c\u7ec6\u80de\u5185\u5747\u8fbe\u5230\u4e86\u5f88\u597d\u7684\u6548\u679c\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u8fd9\u4e2a\u5de5\u4f5c\u5bf9\u540e\u7eedGPCR\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5f88\u597d\u7684\u5de5\u5177\u3002<\/p>\n

\"\"<\/p>\n

\u57fa\u4e8e\u751f\u7269\u6b63\u4ea4\u5316\u5b66\u7684\u8367\u5149\u6210\u50cf<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1ahttp:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/jacs.7b05674<\/a><\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u4e13\u680f\u79d1\u6280\u987e\u95eegaxy\u4f9b\u7a3f\uff0c\u6750\u6599\u4eba\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

\u6750\u6599\u4eba\u62db\u52df\u4e13\u680f\u79d1\u6280\u987e\u95ee\uff0c\u916c\u52b3\u4e30\u539a\uff08\u6587\u5b57200-300\u5143+\u9605\u8bfb\u91cf\u5956\u52b1\uff1b\u89c6\u9891500-1000\u5143\u4e00\u6761\uff09\uff0c\u6709\u610f\u8005\u8bf7\u4e0e\u5ba2\u670d\u5fae\u4fe1cailiaokefu\u8054\u7cfb\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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