{"id":128540,"date":"2018-03-11T06:30:25","date_gmt":"2018-03-10T22:30:25","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=128540"},"modified":"2018-03-10T16:38:14","modified_gmt":"2018-03-10T08:38:14","slug":"%e5%82%ac%e5%8c%96%e6%b4%bb%e6%80%a7%e5%8d%95%e9%93%be%e8%81%9a%e5%90%88%e7%89%a9%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90%ef%bc%9a%e6%8e%a2%e7%b4%a2%e5%85%b6%e5%9c%a8%e5%a4%8d%e6%9d%82%e7%94%9f%e7%89%a9","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=128540","title":{"rendered":"\u50ac\u5316\u6d3b\u6027\u5355\u94fe\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\uff1a\u63a2\u7d22\u5176\u5728\u590d\u6742\u751f\u7269\u5a92\u4f53\u4e2d\u7684\u529f\u80fd"},"content":{"rendered":"<p><strong>\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span title=\"Dynamic single-chain polymeric nanoparticles (SCPNs) are intriguing, bioinspired architectures that result from the collapse or folding of an individual polymer chain into a nanometer-sized particle.\">\u52a8\u6001\u5355\u94fe\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\uff08SCPNs\uff09\u662f\u5f15\u4eba\u6ce8\u76ee\u7684\u751f\u7269\u611f\u5e94\u7ed3\u6784\uff0c\u7531\u5355\u4e2a\u805a\u5408\u7269\u94fe\u6298\u53e0\u6216\u6298\u53e0\u6210\u7eb3\u7c73\u5c3a\u5bf8\u7684\u7c92\u5b50\u800c\u5f62\u6210\u3002\u672c\u6587<\/span><span title=\"Here we present a detailed\n\">\u63d0\u4f9b\u4e86\u4e00\u4e2a\u8be6\u7ec6\u7684<\/span><span title=\"biophysical study on the behavior of dynamic SCPNs in living cells and an evaluation of their catalytic functionality in such a complex medium.\">\u5173\u4e8e\u52a8\u6001SCPN\u5728\u6d3b\u7ec6\u80de\u4e2d\u884c\u4e3a\u7684\u751f\u7269\u7269\u7406\u5b66\u7814\u7a76\u4ee5\u53ca\u5728\u8fd9\u79cd\u590d\u6742\u4ecb\u8d28\u4e2d\u5bf9\u5176\u50ac\u5316\u529f\u80fd\u7684\u8bc4\u4f30\u3002<\/span><span title=\"We first developed a number of delivery strategies that allowed the selective localization of SCPNs in different cellular compartments.\">\u7814\u7a76\u4eba\u5458\u9996\u5148\u5236\u5b9a\u4e86\u8bb8\u591a\u4ea4\u4ed8\u5b9e\u9a8c\uff0c\u5141\u8bb8SCPNs\u5728\u4e0d\u540c\u7684\u7ec6\u80de\u533a\u5ba4\u9009\u62e9\u6027\u5b9a\u4f4d\u3002<\/span><span title=\"Live\/dead tests showed that\n\">\u6d3b\/\u6b7b\u5b9e\u9a8c\u8868\u660e<\/span><span title=\"the SCPNs were not toxic to cells while spectral imaging revealed that SCPNs provide a structural shielding and reduced the influence from the outer biological media.\">SCPN\u5bf9\u7ec6\u80de\u6ca1\u6709\u6bd2\u6027\uff0c\u800c\u5149\u8c31\u6210\u50cf\u663e\u793aSCPNs\u63d0\u4f9b\u7684\u7ed3\u6784\u53ef\u4ee5\u5c4f\u853d\u5e76\u51cf\u5c11\u6765\u81ea\u5916\u90e8\u751f\u7269\u4ecb\u8d28\u7684\u5f71\u54cd\u3002 <\/span><span title=\"The ability of SCPNs to act as catalysts in biological media was first assessed by investigating their potential for reactive oxygen species generation.\">SCPNs\u4f5c\u4e3a\u751f\u7269\u4ecb\u8d28\u50ac\u5316\u5242\u7684\u80fd\u529b\u9996\u5148\u901a\u8fc7\u8c03\u67e5\u5176\u6d3b\u6027\u6c27\u4ea7\u751f\u7684\u6f5c\u529b\u6765\u8bc4\u4f30\u3002<\/span><span title=\"With porphyrins covalently attached to the SCPNs, singlet oxygen was generated upon irradiation with light, inducing spatially controlled cell death.\">\u968f\u7740\u535f\u5549\u4e0eSCPN\u5171\u4ef7\u8fde\u63a5\uff0c\u7528\u5149\u7167\u5c04\u4ea7\u751f\u5355\u7ebf\u6001\u6c27\uff0c\u8bf1\u5bfc\u7a7a\u95f4\u63a7\u5236\u7684\u7ec6\u80de\u6b7b\u4ea1\u3002<\/span><span title=\"In addition, Cu(I)- and Pd(II)-based SCPNs were prepared and these catalysts were screened in vitro and studied in cellular environments for the carbamate cleavage reaction of rhodamine-based substrates.\">\u6b64\u5916\uff0c\u8fd8\u5236\u5907\u4e86Cu\uff08I\uff09 &#8211; \u548cPd\uff08II\uff09- \u57faSCPNs\uff0c\u5e76\u5bf9\u8fd9\u4e9b\u50ac\u5316\u5242\u8fdb\u884c\u4e86\u4f53\u5916\u7b5b\u9009\uff0c\u5e76\u5728\u7ec6\u80de\u73af\u5883\u4e2d\u7814\u7a76\u4e86\u7f57\u4e39\u660e\u57fa\u5e95\u7269\u7684\u6c28\u57fa\u7532\u9178\u916f\u88c2\u89e3\u53cd\u5e94\u3002<\/span><span title=\"This is a model reaction for the uncaging of bioactive compounds such as cytotoxic drugs for catalysis-based cancer therapy.\">\u8fd9\u662f\u7528\u4e8e\u89e3\u9664\u751f\u7269\u6d3b\u6027\u5316\u5408\u7269\u4f8b\u5982\u7ec6\u80de\u6bd2\u6027\u836f\u7269\u7528\u4e8e\u57fa\u4e8e\u50ac\u5316\u7684\u764c\u75c7\u6cbb\u7597\u7684\u6a21\u578b\u53cd\u5e94\u3002\u53ef\u4ee5<\/span><span title=\"We observed that the rate of the deprotection depends on both the organometallic catalysts and the nature of the protective group.\">\u89c2\u5bdf\u5230\uff0c\u8131\u4fdd\u62a4\u901f\u7387\u53d6\u51b3\u4e8e\u6709\u673a\u91d1\u5c5e\u50ac\u5316\u5242\u548c\u4fdd\u62a4\u57fa\u56e2\u7684\u6027\u8d28\u3002<\/span><span title=\"The rate reduces from in vitro to the biological environment, indicating a strong influence of biomolecules on catalyst performance.\">\u4ece\u4f53\u5916\u5230\u751f\u7269\u73af\u5883\u7684\u901f\u7387\u964d\u4f4e\uff0c\u8868\u660e\u751f\u7269\u5206\u5b50\u5bf9\u50ac\u5316\u5242\u6027\u80fd\u7684\u5f3a\u70c8\u5f71\u54cd\u3002<\/span><span title=\"The Cu(I)-based SCPNs in combination with the dimethylpropargyloxycarbonyl protective group showed the best performances both in vitro and in biological environment, making this group promising in biomedical applications.\">\u57fa\u4e8eCu\uff08I\uff09\u7684SCPN\u4e0e\u4e8c\u7532\u57fa\u7094\u4e19\u6c27\u57fa\u7fb0\u57fa\u4fdd\u62a4\u57fa\u7ec4\u5408\u5728\u4f53\u5916\u548c\u751f\u7269\u73af\u5883\u4e2d\u8868\u73b0\u51fa\u6700\u597d\u7684\u6027\u80fd\uff0c\u4f7f\u5f97\u8be5\u7ec4\u5728\u751f\u7269\u533b\u5b66\u5e94\u7528\u4e2d\u6709\u524d\u666f\u3002<\/span><\/span><\/p>\n<p><strong>\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span title=\"In this work, we report our attempt to explore efficient gain media with superior thermal, optical, and electrical properties to address the challenges for OSLs.\">\u8fd1\u65e5\uff0c<strong>\u57c3\u56e0\u970d\u82ac\u7406\u5de5\u5927\u5b66\u7684\u00a0Anja R. A. Palmans <\/strong>\u548c<strong>\u52a0\u6cf0\u7f57\u5c3c\u4e9a\u751f\u7269\u7814\u7a76\u6240<\/strong>\u7684<strong>Lorenzo Albertazzi<\/strong>\u5728<strong>Journal of the American Chemical Society<\/strong>\u4e0a\u53d1\u8868\u4e86\u4e00\u7bc7\u9898\u4e3a\u201c<strong>Catalytically Active Single-Chain Polymeric Nanoparticles: Exploring Their Functions in Complex Biological Media<\/strong>\u201d\u7684\u6587\u7ae0\u3002<span title=\"Here we present a biophysical study of SCPN behavior in living cells and an evaluation of their performances in catalysis in complex biological environments.\">\u8be5\u6587\u4ecb\u7ecd\u4e86SCPN\u5728\u6d3b\u7ec6\u80de\u4e2d\u884c\u4e3a\u7684\u751f\u7269\u7269\u7406\u5b66\u7814\u7a76\uff0c\u4ee5\u53ca\u4ed6\u4eec\u5728\u590d\u6742\u751f\u7269\u73af\u5883\u4e2d\u50ac\u5316\u6027\u80fd\u7684\u8bc4\u4f30\u3002<\/span><span title=\"In our design (Figure 1), the SCPNs comprise a polyacrylamide-based backbone functionalized with (i) water-soluble side chains (oligo-(ethylene oxide-co-propylene oxide) of DP = 22, Jeffamine@M-1000) to\">SCPN\u5305\u542b\u7528\uff08i\uff09\u6c34\u6eb6\u6027\u4fa7\u94fe\uff08DP = 22\u7684\u4f4e\u805a &#8211; \uff08\u6c27\u5316\u4e59\u70ef &#8211; \u5171 &#8211; \u6c27\u5316\u4e19\u70ef\uff09\uff09\u5b98\u80fd\u5316\u7684\u57fa\u4e8e\u805a\u4e19\u70ef\u9170\u80fa\u7684\u9aa8\u67b6<\/span><span title=\"ensure water solubility;\">\u786e\u4fdd\u6c34\u6eb6\u6027; <\/span><span title=\"(ii) benzene-1,3,5-tricarboxamide (BTA) supramolecular moieties in order to trigger hydrogen-bonding-induced polymer folding and (iii) catalytically active sites.\">\uff08ii\uff09\u82ef-1,3,5-\u4e09\u7532\u9170\u80fa\uff08BTA\uff09\u8d85\u5206\u5b50\u90e8\u5206\u4ee5\u5f15\u53d1\u6c22\u952e\u8bf1\u5bfc\u7684\u805a\u5408\u7269\u6298\u53e0\u548c\uff08iii\uff09\u50ac\u5316\u6d3b\u6027\u4f4d\u70b9\u3002<\/span><span title=\"We first assess and optimize delivery strategies in order to target different compartments: the intracellular space, the cytoplasm or the endolysosomal compartment, and the extracellular space.\">\u9996\u5148\u8bc4\u4f30\u548c\u4f18\u5316\u9012\u9001\u7b56\u7565\uff0c\u4ee5\u9488\u5bf9\u4e0d\u540c\u7684\u533a\u5ba4\uff1a\u7ec6\u80de\u5185\u7a7a\u95f4\uff0c\u7ec6\u80de\u8d28\u6216\u80de\u8d28\u6eb6\u8d28\u4f53\u5ba4\u548c\u7ec6\u80de\u5916\u7a7a\u95f4\u3002<\/span><span title=\"This allows us to\n\">\u8fd9\u4f7f\u7814\u7a76\u4eba\u5458\u80fd\u591f<\/span><span title=\"choose the delivery strategy depending on the desired applications.\">\u6839\u636e\u6240\u9700\u7684\u5e94\u7528\u9009\u62e9\u4ea4\u4ed8\u7b56\u7565\u3002<\/span><span title=\"Next, we evaluate two activities to be performed in the cellular environment as shown in Scheme 1: (i) the photocatalytic generation of singlet oxygen ( 1 O 2 ) from\n\">\u63a5\u4e0b\u6765\uff0c\u8bc4\u4f30\u4e86\u4e24\u79cd\u5728\u7ec6\u80de\u73af\u5883\u4e2d\u8fdb\u884c\u7684\u6d3b\u52a8\uff0c\u5b9e\u9a8c\u7ed3\u679c\u5f88\u6709\u524d\u666f<\/span><span title=\"Both approaches give promising results, paving the way toward catalysis in vivo for biomedical applications.\">\uff0c\u4e3a\u751f\u7269\u533b\u5b66\u5e94\u7528\u7684\u4f53\u5185\u50ac\u5316\u94fa\u5e73\u4e86\u9053\u8def\u3002<\/span><span title=\"Moreover, our biophysical studies of SCPN behavior in living cells provides crucial information and will enable the rational design of improved nanosystems for catalysis-based therapies.\">\u6b64\u5916\uff0c\u5173\u4e8e\u6d3b\u7ec6\u80de\u4e2dSCPN\u884c\u4e3a\u7684\u751f\u7269\u7269\u7406\u5b66\u7814\u7a76\u63d0\u4f9b\u4e86\u5173\u952e\u4fe1\u606f\uff0c\u5e76\u5c06\u4f7f\u5f97\u80fd\u591f\u5408\u7406\u8bbe\u8ba1\u6539\u8fdb\u7684\u7528\u4e8e\u57fa\u4e8e\u50ac\u5316\u7597\u6cd5\u7684\u7eb3\u7c73\u7cfb\u7edf\u3002<\/span><\/span><\/span><\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>\u56fe1 SCPNs\u8bbe\u8ba1\u56fe<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128558 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/1-9.png\" alt=\"\" width=\"700\" height=\"399\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/1-9.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/1-9-300x171.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08A\uff09\u805a\u5408\u7269\u50ac\u5316\u5242\u7684\u4e00\u822c\u7ed3\u6784\uff0c\u5176\u6298\u53e0\u6210SCPN\u53ca\u5176\u7ec6\u80de\u9012\u9001\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> <span class=\"\">\uff08B\uff09\u672c\u7814\u7a76\u4e2d\u4f7f\u7528\u7684\u6c34\u6eb6\u6027\u4fa7\u94fe\uff0c\u57fa\u4e8eBTA\u7684\u6298\u53e0\u6a21\u4f53\uff0c\u50ac\u5316\u5242\u548c\u5e95\u7269\u7684\u5316\u5b66\u7ed3\u6784\u3002<\/span><\/span><\/p>\n<p><strong>\u56fe2 <span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">Hela\u7ec6\u80de\u7684\u5171\u805a\u7126\u6210\u50cf\u4f7f\u7528\u4e0d\u540c\u7684\u65b9\u6cd5\u6765\u8fdb\u884c\u57fa\u4e8eP1\u7684SCPN\u9012\u9001<\/span><\/span><br \/>\n <\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128557 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/2-10.png\" alt=\"\" width=\"700\" height=\"470\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/2-10.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/2-10-300x201.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\">\uff08A\uff09\u901a\u8fc7\u4ee5\u9ad8\u6d53\u5ea6\uff082.5 mg\u00b7mL<sup>-1<\/sup>\uff09\u5728\u57f9\u517b\u57fa\u4e2d\u901a\u8fc7\u80de\u541e\u4f5c\u7528\u548c\u968f\u540e\u7684P<sub>1<\/sub>\u7684\u6eb6\u9176\u4f53\u5b9a\u4f4d\u65bd\u7528\u7ec6\u80de\u5185\u531624\u5c0f\u65f6\uff1b<\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> \uff08B\uff09\u901a\u8fc7\u7535\u7a7f\u5b54\u7ec6\u80de\u5185\u9012\u9001\u548c\u968f\u540eP1\u7684\u80de\u8d28\u5b9a\u4f4d\uff1b<\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08C\uff09\u901a\u8fc7\u5411\u57f9\u517b\u57fa\u4e2d\u65bd\u7528P<sub>1<\/sub> 1.0mg\/mL\u5904\u74063\u5c0f\u65f6\u540e\u8fdb\u884c\u7ec6\u80de\u5916\u5b9a\u4f4d\u3002<\/span><\/span><\/p>\n<p><strong>\u56fe3\u00a0<span id=\"result_box\" class=\"short_text\" lang=\"zh-CN\"><span class=\"\">\u7528\u6d3b\/\u6b7b\u5b9e\u9a8c\u6d4b\u5b9aP<sub>1<\/sub>\u7684\u6bd2\u6027<\/span><\/span><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128556 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/3-10.png\" alt=\"\" width=\"700\" height=\"389\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/3-10.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/3-10-300x167.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08A\uff09\u5728SCPN\u5185\u5316\u540eHeLa\u7ec6\u80de\u7684\u7ec6\u80de\u5b58\u6d3b\u529b\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08B\uff09\u672a\u5904\u7406\u7ec6\u80de\u7684\u5b58\u6d3b\u529b\u4f5c\u4e3a\u5bf9\u7167\uff1b<\/span> <\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08C\uff09\u7535\u7a7f\u5b54\u533a\u57df\u7684\u6210\u50cf\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> <span class=\"\">\uff08D\uff09\u53d6\u51b3\u4e8e\u4e0e\u7535\u6781\u7684\u8ddd\u79bb\u7684\u7ec6\u80de\u751f\u5b58\u529b\u7684\u7a7a\u95f4\u5206\u5e03\u3002<\/span>\u00a0<\/span><\/p>\n<p><strong>\u56fe4\u00a0<span id=\"result_box\" class=\"short_text\" lang=\"zh-CN\"><span class=\"\"><span id=\"result_box\" class=\"\" lang=\"zh-CN\">\u5728\u7528\u4e0d\u540c\u6d53\u5ea6\u7684\u57fa\u4e8eP<sub>3<\/sub>\u7684SCPN\u6e29\u80b224\u5c0f\u65f6\u540eHeLa\u7ec6\u80de\u4e2d\u5355\u7ebf\u6001\u6c27\u7684\u5149\u751f\u6210<\/span><\/span><\/span><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128553 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/4-10.png\" alt=\"\" width=\"700\" height=\"525\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/4-10.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/4-10-300x225.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\">\uff08A\uff09\u7528\u4e0d\u540c\u6d53\u5ea6\u7684P3\u5904\u7406\u7684HeLa\u7ec6\u80de\u7684\u5171\u7126\u6210\u50cf\u663e\u793a\u6d53\u5ea6\u4f9d\u8d56\u6027\u6444\u53d6\uff1b<\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> \uff08B\uff09\u6d3b\/\u6b7b\u7684\u5171\u7126\u6210\u50cf\uff0c<span class=\"\">\u5bf9\u7528P1\uff08\u5de6\uff09\u548cP3\uff08\u53f3\uff09\u5904\u7406\u7684HeLa\u7ec6\u80de\u8fdb\u884c\u6d4b\u5b9a\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> <span class=\"\">\uff08C\uff09\u5728\u4e0d\u540c\u805a\u5408\u7269\u6d53\u5ea6\u548c30\u548c150 s\u7684UV\u7167\u5c04\u65f6\u95f4\u4e0b\u7ec6\u80de\u6d3b\u529b\u7684\u5b9a\u91cf\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> <span class=\"\">\uff08D\uff09\u4e0d\u540c\u805a\u5408\u7269\u6d53\u5ea6\u7684\u76f8\u5e94\u5171\u7126\u6d3b\/\u6b7b\u6d4b\u5b9a\u3002<\/span> <span class=\"\">\u6bd4\u4f8b\u5c3a= 50\u03bcm\u3002<\/span><\/span><\/p>\n<p><strong>\u56fe5\u00a0<span id=\"result_box\" class=\"short_text\" lang=\"zh-CN\"><span class=\"\"><span id=\"result_box\" class=\"\" lang=\"zh-CN\">\u5728\u5b58\u5728HeLa\u7ec6\u80de\u7684\u60c5\u51b5\u4e0bSCPN\u57fa\u50ac\u5316\u5242\u5bf9\u53d7\u4fdd\u62a4\u7684\u7f57\u4e39\u80fa\u8fdb\u884c\u8131\u4fdd\u62a4\u53cd\u5e94\u7684\u7ed3\u679c<\/span><\/span><\/span><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128555 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/5-9.png\" alt=\"\" width=\"700\" height=\"287\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/5-9.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/5-9-300x123.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08A\uff09\u672c\u7814\u7a76\u4e2d\u4f7f\u7528\u7684\u57fa\u6750S1-S4\u7684\u5316\u5b66\u7ed3\u6784\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> <span class=\"\">\uff08B\uff0cC\uff09S2\uff08B\uff09\u548cS1\uff08C\uff09\u4e0e\u57fa\u4e8eP2 Cu\uff08I\uff09\u548cP1 Pd\uff08II\uff09\u7684SCPN\u7684\u8131\u4fdd\u62a4\u53cd\u5e94\u7684\u52a8\u529b\u5b66\u66f2\u7ebf\u5728\u5b58\u5728\u7ec6\u80de\u57f9\u517b\u57fa\u548cHeLa<\/span> \u7ec6\u80de\uff1b <\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"> \uff08D\uff09\u76f4\u65b9\u56fe\u603b\u7ed3\uff0c<span class=\"\">\u4e0d\u540c\u50ac\u5316\u5242\u3001\u5e95\u7269\u548c\u5bf9\u7167\u53cd\u5e942\u5c0f\u65f6\u540eMC-Rh 110\u7684\u8367\u5149\u5f3a\u5ea6\u3002<\/span><\/span><\/p>\n<p><strong>\u56fe6\u00a0<span id=\"result_box\" class=\"\" lang=\"zh-CN\">\u5728\u590d\u6742\u4ecb\u8d28\u4e2d\u4f7f\u7528SCPN\u8fdb\u884c48\u5c0f\u65f6\u7684\u8131\u4fdd\u62a4\u53cd\u5e94\u52a8\u529b\u5b66<\/span><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-128554 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/6-6.png\" alt=\"\" width=\"700\" height=\"655\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/6-6.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/6-6-300x281.png 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08A\uff0cB\uff09\u5728\u5168\u7ec6\u80de\u57f9\u517b\u57fa\uff08DMEM + 10\uff05\u8840\u6e05\uff09\u4e2d\uff0c\u5728\u6709\u6216\u6ca1\u6709HeLa\u7ec6\u80de\u7684\u60c5\u51b5\u4e0b\uff0cS<sub>2<\/sub>\uff08B\uff09\u7684S<sub>1<\/sub>\uff08A\uff09\u548cP1 @ Pd\uff08II\uff09\u7684P2 Cu\uff08I\uff09\u50ac\u5316\u8131\u7094\u4e8c\u5316\u53cd\u5e94\uff1b<\/span><\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\">\u00a0<\/span><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span class=\"\">\uff08C\uff0cD\uff09SCPN\u4e0e\u91d1\u5c5e\u6216\u4ec5\u4e0e\u91d1\u5c5e\u590d\u5408\uff0c\u4e0e\u5e95\u7269\u4e00\u8d77\u5b75\u80b2\u7684HeLa\u7ec6\u80de\u7684\u6210\u50cf<\/span>\u3002<br \/>\n <\/span><\/p>\n<p><strong>\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"zh-CN\"><span title=\"In this work, we show the results of a biophysical study of dynamic single-chain catalytic polymeric nanoparticle activity in a biological environment.\">\u5728\u8fd9\u9879\u5de5\u4f5c\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u5c55\u793a\u4e86\u751f\u7269\u73af\u5883\u4e2d\u52a8\u6001\u5355\u94fe\u50ac\u5316\u805a\u5408\u7269\u7eb3\u7c73\u9897\u7c92\u6d3b\u6027\u7684\u751f\u7269\u7269\u7406\u5b66\u7814\u7a76\u7ed3\u679c\u3002<\/span><span title=\"The SCPNs, even those comprising transition-metal-based catalysts, showed excellent biocompatibility and did not show significant toxicity toward cells.\">SCPNs\u751a\u81f3\u5305\u542b\u8fc7\u6e21\u91d1\u5c5e\u57fa\u50ac\u5316\u5242\u7684\u90a3\u4e9b\u90fd\u8868\u73b0\u51fa\u4f18\u5f02\u7684\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u5e76\u4e14\u4e0d\u663e\u793a\u5bf9\u7ec6\u80de\u7684\u663e\u7740\u6bd2\u6027\u3002<\/span><span title=\"In addition, evidence is presented that the dynamic SCPNs create a rather stable environment for the catalytic species.\">\u6b64\u5916\uff0c\u6709\u8bc1\u636e\u8868\u660e\uff0c\u52a8\u6001SCPN\u4e3a\u50ac\u5316\u7269\u79cd\u521b\u9020\u4e86\u4e00\u4e2a\u76f8\u5f53\u7a33\u5b9a\u7684\u73af\u5883\u3002<\/span><span title=\"Two strategies to assess the viability of the use of SCPNs in complex biological media were investigated.\">\u7814\u7a76\u4e86\u4e24\u79cd\u8bc4\u4f30\u5728\u590d\u6742\u751f\u7269\u4ecb\u8d28\u4e2d\u4f7f\u7528SCPN\u7684\u53ef\u884c\u6027\u7684\u7b56\u7565\u3002<\/span><span title=\"induced localized toxicity when porphyrin-based SCPNs were introduced via endocytosis into cells.\"><span title=\"Irradiation with light successfully generated singlet oxygen and promoted light-induced localized toxicity when porphyrin-based SCPNs were introduced via endocytosis into cells.\">\u5f53\u901a\u8fc7\u5185\u541e\u4f5c\u7528\u5c06\u535f\u5549\u57faSCPN\u5f15\u5165\u7ec6\u80de\u65f6\uff0c\u7528\u5149\u7167\u5c04\u6210\u529f\u4ea7\u751f\u5355\u7ebf\u6001\u6c27\u5e76\u4fc3\u8fdb\u5149\u81f4\u5c40\u90e8\u6bd2\u6027\u3002<\/span><\/span><span title=\"This makes these SCPNs highly interesting for future applications in photodynamic therapy.\">\u8fd9\u4f7f\u5f97\u8fd9\u4e9bSCPN\u5bf9\u672a\u6765\u5728\u5149\u52a8\u529b\u7597\u6cd5\u4e2d\u7684\u5e94\u7528\u975e\u5e38\u6709\u8da3\u3002<\/span><span title=\"In addition, Pd(II) and Cu(I) loaded metal-based SCPNs showed an efficient depropargylation of protected rhodamine in the extracellular space.\">\u6b64\u5916\uff0c\u88c5\u8f7dPd\uff08II\uff09\u548cCu\uff08I\uff09\u7684\u91d1\u5c5e\u57faSCPN\u5728\u7ec6\u80de\u5916\u7a7a\u95f4\u4e2d\u663e\u793a\u51fa\u6709\u6548\u7684\u8131\u4fdd\u62a4\u7684\u7f57\u4e39\u660e\u4fdd\u62a4\u4f5c\u7528\u3002<\/span><span title=\"The development of catalytic systems that are able to perform their functions in living cells has been a subject of recent attention because of its potential applications in the fields of synthetic biology and therapeutic biomaterials.\">\u7531\u4e8eSCPN\u5728\u5408\u6210\u751f\u7269\u5b66\u548c\u6cbb\u7597\u6027\u751f\u7269\u6750\u6599\u9886\u57df\u7684\u6f5c\u5728\u5e94\u7528\uff0c\u80fd\u591f\u5728\u6d3b\u7ec6\u80de\u4e2d\u53d1\u6325\u5176\u529f\u80fd\u7684\u50ac\u5316\u7cfb\u7edf\u7684\u53d1\u5c55\u4e00\u76f4\u662f\u8fd1\u6765\u5173\u6ce8\u7684\u4e3b\u9898\u3002<\/span><span title=\"However, the rational design of such structures is a huge chemical challenge, mostly due to the lack of knowledge on the behavior of catalytic systems in the complex biological environment and therefore on their structure\u2212activity relations.\">\u7136\u800c\uff0c\u8fd9\u79cd\u7ed3\u6784\u7684\u5408\u7406\u8bbe\u8ba1\u662f\u4e00\u4e2a\u5de8\u5927\u7684\u5316\u5b66\u6311\u6218\uff0c\u4e3b\u8981\u662f\u7531\u4e8e\u7f3a\u4e4f\u5bf9\u590d\u6742\u751f\u7269\u73af\u5883\u4e2d\u50ac\u5316\u4f53\u7cfb\u884c\u4e3a\u7684\u4e86\u89e3\uff0c\u56e0\u6b64\u7f3a\u4e4f\u5bf9\u5176\u7ed3\u6784 &#8211; \u6d3b\u6027\u5173\u7cfb\u7684\u4e86\u89e3\u3002<\/span><span title=\"Therefore, the biophysical study presented here, sheds light on the behavior of SCPNs in the cellular environment, paving the way toward the rational design of nanosystems that are able to perform effective catalysis in vivo.\">\u56e0\u6b64\uff0c\u672c\u6587\u4ecb\u7ecd\u7684\u751f\u7269\u7269\u7406\u5b66\u7814\u7a76\u63ed\u793a\u4e86SCPN\u5728\u7ec6\u80de\u73af\u5883\u4e2d\u7684\u884c\u4e3a\uff0c\u4e3a\u5408\u7406\u8bbe\u8ba1\u80fd\u591f\u5728\u4f53\u5185\u8fdb\u884c\u6709\u6548\u50ac\u5316\u7684\u7eb3\u7c73\u7cfb\u7edf\u94fa\u5e73\u4e86\u9053\u8def\u3002<\/span><\/span><\/p>\n<p><strong>\u6587\u732e\u94fe\u63a5<\/strong>\uff1a<a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.8b00122\" target=\"_blank\" rel=\"noopener\">Catalytically Active Single-Chain Polymeric Nanoparticles: Exploring Their Functions in Complex Biological Media<\/a>\uff08<em>J. Am. Chem. Soc.<\/em>\uff0c2018\uff0cDOI:10.1021\/jacs.8b00122\uff09<\/p>\n<p>\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u9ad8\u5206\u5b50\u5b66\u672f\u7ec4\u6c34\u624b\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n<p>\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c<a href=\"http:\/\/www.cailiaoniu.com\/25127.html\" target=\"_blank\" rel=\"noopener\"><strong>\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/strong><\/a>\u3002\u53c2\u4e0e\u9ad8\u5206\u5b50\u8bdd\u9898\u8ba8\u8bba\u6216\u4e86\u89e3\u9ad8\u5206\u5b50\u7ec4\u62db\u52df\u8be6\u60c5\uff0c\u8bf7\u52a0\u9ad8\u5206\u5b50\u4ea4\u6d41\u7fa4\uff08298064863\uff09\u3002<\/p>\n<p><strong>\u6750\u6599\u6d4b\u8bd5\u3001\u6570\u636e\u5206\u6790\uff0c\u4e0a<\/strong><a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\" rel=\"noopener\"><strong>\u6d4b\u8bd5\u8c37<\/strong><\/a><strong>\uff01<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5f15\u8a00\u3011 \u52a8\u6001\u5355\u94fe\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\uff08SCPNs\uff09\u662f\u5f15\u4eba\u6ce8\u76ee\u7684\u751f\u7269\u611f\u5e94\u7ed3\u6784\uff0c\u7531\u5355\u4e2a\u805a\u5408\u7269\u94fe\u6298\u53e0\u6216\u6298\u53e0\u6210\u7eb3\u7c73\u5c3a\u5bf8\u7684\u7c92\u5b50\u800c\u5f62\u6210\u3002\u672c\u6587\u63d0\u4f9b\u4e86\u4e00\u4e2a\u8be6\u7ec6\u7684\u5173\u4e8e\u52a8\u6001SCPN\u5728\u6d3b&#8230;<\/p>\n","protected":false},"author":3247,"featured_media":128558,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[75],"class_list":["post-128540","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-biomaterial"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/128540"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/3247"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=128540"}],"version-history":[{"count":3,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/128540\/revisions"}],"predecessor-version":[{"id":129472,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/128540\/revisions\/129472"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/media\/128558"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=128540"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=128540"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=128540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}