{"id":130724,"date":"2018-03-29T06:30:34","date_gmt":"2018-03-28T22:30:34","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=130724"},"modified":"2018-03-28T22:12:08","modified_gmt":"2018-03-28T14:12:08","slug":"%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88%e5%9c%a8%e8%b6%85%e5%88%86%e8%be%a8%e6%88%90%e5%83%8f%e4%b8%ad%e7%9a%84%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=130724","title":{"rendered":"\u3010\u6d4b\u8bd5\u6280\u672f\u4e13\u680f\u3011\u8367\u5149\u63a2\u9488\u5728\u8d85\u5206\u8fa8\u6210\u50cf\u4e2d\u7684\u5e94\u7528"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-131967\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/ceshi-s.png\" alt=\"\" width=\"700\" height=\"388\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/ceshi-s.png 700w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/ceshi-s-300x166.png 300w\" 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Abbe\u7528\u6570\u5b66\u516c\u5f0f\u63a8\u5bfc\u548c\u8868\u8fbe\u4e86\u5149\u5b66\u884d\u5c04\u6781\u9650<\/p>\n<p>\u901a\u5e38\u901a\u7528\u7269\u955c\u7684NA\u6700\u5927\u503c\u4e3a1.49\uff0c\u56e0\u800c\u53ef\u4ee5\u63a8\u7b97\u51fa\u5bf9\u4e8e\u53ef\u89c1\u5149\uff0c\u5176\u5206\u8fa8\u7387\u5927\u6982\u4e3a200\u7eb3\u7c73\u3002\u56e0\u6b64\u53ef\u4ee5\u8ba4\u4e3a200\u7eb3\u7c73\u4e3a\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u5206\u8fa8\u7387\u6781\u9650\uff0c200\u7eb3\u7c73\u4ee5\u5185\u7684\u5fae\u89c2\u7ed3\u6784\u90fd\u65e0\u6cd5\u89c2\u5bdf\u3002<\/p>\n<p>\u8981\u8fbe\u5230\u5149\u5b66\u884d\u5c04\u6781\u9650\uff0c\u9700\u8981\u6ee1\u8db3\u4e24\u4e2a\u6761\u4ef6\u3002\u9996\u5148\u8981\u6c42\u7269\u4f53\u4e0e\u50cf\u5e73\u9762\u7684\u8ddd\u79bb\u8981\u8fdc\u5927\u4e8e\u5149\u7684\u6ce2\u957f\uff1b\u5176\u6b21\u70b9\u5149\u6e90\u8981\u540c\u65f6\u53d1\u5149\uff0c\u8fd9\u6837\u884d\u5c04\u5149\u6591\u624d\u53ef\u4ee5\u53e0\u52a0\u5728\u4e00\u8d77\u3002\u56e0\u6b64\uff0c\u53ea\u8981\u5149\u5b66\u63a2\u9488\u8ddd\u79bb\u7269\u4f53\u7684\u8ddd\u79bb\u8fdc\u5c0f\u4e8e\u5149\u6ce2\u957f\uff0c\u6216\u8005\u70b9\u5149\u6e90\u5728\u4e0d\u540c\u65f6\u95f4\u5206\u522b\u53d1\u5149\uff0c\u5c31\u53ef\u4ee5\u6253\u7834\u884d\u5c04\u6781\u9650\uff0c\u5b9e\u73b0\u8d85\u5206\u8fa8\u6210\u50cf\u3002<\/p>\n<p><strong><b>2.<\/b><\/strong><strong><b>\u8d85\u5206\u8fa8\u6210\u50cf\u6280\u672f<\/b><\/strong><\/p>\n<p>\u5e38\u89c4\u6240\u7528\u5230\u7684\u8d85\u5206\u8fa8\u6210\u50cf\u6280\u672f\u4e3b\u8981\u5206\u4e3a\u4e24\u7c7b\u3002\u4e00\u7c7b\u662f\u57fa\u4e8e\u8367\u5149\u5355\u5206\u5b50\u7684\u7cbe\u786e\u5b9a\u4f4d\u3002\u6bd4\u5982\u7531\u5e84\u5c0f\u5a01\u3001Eric Betzig\u7b49\u4eba\u6240\u53d1\u660e\u7684\u8367\u5149\u6d3b\u5316\u5b9a\u4f4d\u663e\u5fae\u6280\u672f\uff08fPALM\uff09\u3001\u5149\u6d3b\u5316\u5b9a\u4f4d\u663e\u5fae\u6280\u672f\uff08PALM\uff09\u548c\u968f\u673a\u5149\u5b66\u91cd\u5efa\u663e\u5fae\u6280\u672f\uff08STORM\uff09\u3002\u8fd9\u4e9b\u65b9\u6cd5\u90fd\u662f\u5229\u7528\u8367\u5149\u5355\u5206\u5b50\u968f\u673a\u7684\u53d1\u5149\uff0c\u901a\u8fc7\u591a\u62cd\u6444\u51e0\u6b21\u7684\u65b9\u6cd5\uff0c\u5c06\u8fd9\u4e9b\u8367\u5149\u5355\u5206\u5b50\u7684\u6fc0\u53d1\u7ed3\u679c\u76f8\u53e0\u52a0\u5f62\u6210\u6574\u4e2a\u56fe\u50cf\uff1b\u7b2c\u4e8c\u7c7b\u662f\u5c06\u884d\u5c04\u6591\u53d8\u5c0f\uff0c\u6bd4\u5982\u53d7\u6fc0\u53d1\u5c04\u635f\u8017\u8367\u5149\u663e\u5fae\u6280\u672f\uff08STED\uff09\u3002STED\u7cfb\u7edf\u9700\u8981\u4e24\u675f\u5149\uff0c\u4e00\u675f\u4e3a\u6fc0\u53d1\u5149\uff0c\u53e6\u4e00\u675f\u4e3a\u635f\u8017\u5149\u3002\u6fc0\u53d1\u5149\u4f7f\u884d\u5c04\u6591\u4ee5\u5185\u7684\u8367\u5149\u5206\u5b50\u4ece\u57fa\u6001\u8dc3\u8fc1\u5230\u6fc0\u53d1\u6001\uff0c\u800c\u635f\u8017\u5149\u5219\u4f7f\u90e8\u5206\u5904\u4e8e\u884d\u5c04\u6591\u5916\u56f4\u7684\u7535\u5b50\u901a\u8fc7\u53d7\u6fc0\u53d1\u5c04\u7684\u65b9\u5f0f\u56de\u5230\u57fa\u6001\u3002\u5904\u4e8e\u5149\u6591\u4e2d\u5fc3\u7684\u7535\u5b50\u4e0d\u53d7\u5f71\u54cd\uff0c\u7ee7\u7eed\u4ee5\u8367\u5149\u7684\u65b9\u5f0f\u56de\u5230\u57fa\u6001\uff0c\u56e0\u6b64\u6709\u6548\u5730\u51cf\u5c11\u4e86\u884d\u5c04\u5149\u6591\u7684\u9762\u79ef\uff0c\u5b9e\u73b0\u4e86\u884d\u5c04\u6781\u9650\u4ee5\u5185\u7684\u6210\u50cf\u3002<\/p>\n<p><strong><b>3.<\/b><\/strong><strong><b>\u7528\u4e8e\u8d85\u5206\u8fa8\u6210\u50cf\u7684\u8367\u5149\u63a2\u9488<\/b><\/strong><\/p>\n<p>\u7531\u4e8e\u8d85\u5206\u8fa8\u8367\u5149\u6210\u50cf\u7684\u5728\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u8fc5\u901f\u53d1\u5c55\uff0c\u9488\u5bf9\u8d85\u5206\u8fa8\u6210\u50cf\u8367\u5149\u63a2\u9488\u7684\u7814\u7a76\u4e0e\u5f00\u53d1\u4e5f\u53d7\u5230\u4e86\u5e7f\u6cdb\u7684\u5173\u6ce8\u3002\u8fd9\u91cc\uff0c\u4e3b\u8981\u4ecb\u7ecd\u4ee5\u4e0b\u51e0\u7c7b\u8367\u5149\u63a2\u9488\u5728\u8d85\u5206\u8fa8\u6210\u50cf\u4e2d\u7684\u5e94\u7528\u3002<\/p>\n<p><strong><b>\uff081\uff09<\/b><\/strong><strong><b>\u57fa\u4e8e\u4e8c\u82b3\u70ef\u548c\u87ba\u5421\u5583\u751f\u8272\u56e2\u7684\u5149\u5b66\u5f00\u5173\u6750\u6599<\/b><\/strong><\/p>\n<p>\u4e8c\u82b3\u70ef\u4f5c\u4e3a\u4e00\u79cd\u5e38\u89c1\u7684\u5149\u81f4\u53d8\u8272\u6750\u6599\u5728\u6570\u636e\u50a8\u5b58\u548c\u5149\u8f6c\u6362\u65b9\u9762\u90fd\u6709\u5e7f\u6cdb\u7684\u7528\u9014\u3002\u901a\u8fc7\u5c06\u82dd\u5355\u9170\u4e9a\u80fa\u548c\u4e8c\u82b3\u70ef\u5171\u4ef7\u8fde\u63a5\uff0c\u53ef\u4ee5\u514b\u670d\u4e8c\u82b3\u70ef\u8367\u5149\u91cf\u5b50\u4ea7\u7387\u8f83\u4f4e\u7684\u7f3a\u70b9\u3002\u6240\u5f62\u6210\u7684\u8367\u5149\u63a2\u9488\u540c\u65f6\u5177\u5907\u4e86\u5149\u8f6c\u6362\u6027\u8d28\u548c\u8f83\u9ad8\u7684\u8367\u5149\u91cf\u5b50\u4ea7\u7387\u3002\u5f53\u4e8c\u82b3\u70ef\u5173\u73af\u65f6\uff0c\u82dd\u5355\u9170\u4e9a\u80fa\u7684\u53d1\u5c04\u5149\u8c31\u548c\u4e8c\u82b3\u70ef\u7684\u5438\u6536\u5149\u8c31\u91cd\u53e0\uff0c\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u4f7f\u82dd\u5355\u9170\u4e9a\u80fa\u7684\u8367\u5149\u88ab\u4e8c\u82b3\u70ef\u731d\u706d\uff1b\u5f53\u4e8c\u82b3\u70ef\u5f00\u73af\u65f6\uff0c\u4e8c\u82b3\u70ef\u7684\u5438\u6536\u5149\u8c31\u79fb\u52a8\uff0c\u4e24\u8005\u4e4b\u95f4\u7684\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u6d88\u5931\uff0c\u82dd\u5355\u9170\u4e9a\u80fa\u53d1\u5149\u3002\u8fd9\u4e2a\u5149\u81f4\u53d8\u8272\u7684\u5206\u5b50\u6210\u529f\u5730\u7528\u5728\u4e86PALM\u6210\u50cf\u4e2d\uff0c\u5206\u8fa8\u7387\u9ad8\u8fbe100\u7eb3\u7c73\uff0c\u5b9e\u73b0\u4e86\u5bf9\u5d4c\u6bb5\u805a\u5408\u7269\u7684\u5fae\u89c2\u7ed3\u6784\u7684\u6210\u50cf\u3002<\/p>\n<p>\u87ba\u5421\u5583\u4f5c\u4e3a\u4e00\u79cd\u5149\u81f4\u53d8\u8272\u6750\u6599\uff0c\u67d3\u6599\u672c\u8eab\u662f\u65e0\u8272\u7684\uff0c\u5728\u7d2b\u5916\u5149\u7167\u5c04\u540e\u5f00\u73af\u5f62\u6210\u4e24\u6027\u79bb\u5b50\u90e8\u82b1\u83c1\u7ed3\u6784\u3002\u540e\u8005\u5728500-650\u7eb3\u7c73\u5177\u6709\u663e\u8457\u7684\u5438\u6536\uff0c\u53d1\u5149\u8303\u56f4\u5728\u7ea2\u5149\u5230\u8fd1\u7ea2\u5916\u533a\u95f4\u3002\u7531\u4e8e\u87ba\u5421\u5583\u7684\u5149\u5b66\u5f00\u5173\u6027\u8d28\uff0c\u6210\u529f\u7528\u4e8ePALM\u6210\u50cf\uff0c\u5e76\u5b9e\u73b0\u4e86\u5bf9\u5d4c\u6bb5\u5171\u805a\u7269\u5fae\u89c2\u7ed3\u6784\u7684\u89c2\u5bdf\u3002<\/p>\n<p><img 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loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-130718\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-EOS.jpg\" alt=\"\" width=\"600\" height=\"267\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-EOS.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-EOS-300x134.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\">Eos\u86cb\u767d\u7684\u4e0d\u53ef\u4ee5\u5149\u8f6c\u79fb<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-130719\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-dopa.jpg\" alt=\"\" width=\"600\" height=\"304\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-dopa.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2018\/03\/revised-dopa-300x152.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\">Dronpa\u86cb\u767d\u7684\u53ef\u9006\u8367\u5149\u5f00\u5173<\/p>\n<p>\u8367\u5149\u8d85\u5206\u8fa8\u6210\u50cf\u6280\u672f\u4e3a\u4eba\u7c7b\u63a2\u7d22\u5fae\u89c2\u9886\u57df\u63d0\u4f9b\u4e86\u6709\u6548\u5730\u9014\u5f84\u548c\u65b9\u6cd5\u3002\u968f\u7740\u79d1\u6280\u7684\u53d1\u5c55\uff0c\u76f8\u4fe1\u8d8a\u6765\u8d8a\u591a\u6027\u80fd\u66f4\u4f18\u8d8a\u7684\u8367\u5149\u6750\u6599\u4f1a\u88ab\u5f00\u53d1\u51fa\u6765\uff0c\u5c4a\u65f6\uff0c\u5fae\u89c2\u9886\u57df\u7684\u7814\u7a76\u6210\u679c\u4f1a\u66f4\u52a0\u70ab\u76ee\u3002<\/p>\n<p>\u53c2\u8003\u6587\u732e\uff1a<\/p>\n<p><a href=\"https:\/\/www.nature.com\/articles\/nrm2531.pdf\" target=\"_blank\" rel=\"noopener\">Fluorescent Probes for Super-Resolution Imaging in Living Cells.\u00a0<\/a><\/p>\n<p>\u672c\u6587\u7531\u6750\u6599\u4eba\u5b66\u672f\u7ec4gaxy\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002 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