{"id":146193,"date":"2018-07-17T14:00:04","date_gmt":"2018-07-17T06:00:04","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=146193"},"modified":"2018-07-15T15:24:16","modified_gmt":"2018-07-15T07:24:16","slug":"%e5%b0%a4%e6%98%8e%e6%97%ad-jacs-%e5%9f%ba%e5%9b%a0%e7%bc%96%e7%a0%81%e7%9a%84%e5%82%ac%e5%8c%96%e5%8f%91%e5%a4%b9%e7%bb%84%e8%a3%85%ef%bc%8c%e7%94%a8%e4%ba%8e%e6%b4%bb%e7%bb%86%e8%83%9e%e4%b8%ad","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=146193","title":{"rendered":"\u5c24\u660e\u65ed JACS: \u57fa\u56e0\u7f16\u7801\u7684\u50ac\u5316\u53d1\u5939\u7ec4\u88c5\uff0c\u7528\u4e8e\u6d3b\u7ec6\u80de\u4e2d\u7684\u7075\u654fRNA\u6210\u50cf"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

DNA\u548cRNA\u6298\u53e0\u6210\u7cbe\u786e\u7eb3\u7c73\u7ed3\u6784\u7684\u80fd\u529b\u5df2\u88ab\u7528\u4e8e\u5404\u79cd\u751f\u7269\u5b66\u548c\u751f\u7269\u533b\u5b66\u9886\u57df\u3002\u8fd9\u4e9b\u6838\u9178\u7cbe\u786e\u81ea\u7ec4\u88c5\u7684\u7eb3\u7c73\u7ed3\u6784\u4e3a\u53ef\u7f16\u7a0b\u5206\u5b50\u8bc6\u522b\u5de5\u5177\u7684\u5f00\u53d1\u521b\u9020\u4e86\u5f88\u597d\u7684\u5e73\u53f0\u3002\u5728\u8fc7\u53bb\u7684\u5341\u5e74\u4e2d\uff0c\u5df2\u7ecf\u8bbe\u8ba1\u4e86\u4e00\u7cfb\u5217\u590d\u6742\u7684\u57fa\u4e8e\u6838\u9178\u7684\u7535\u8def\u5e76\u5e94\u7528\u4e8e\u751f\u7269\u5206\u6790\u68c0\u6d4b\u3002\u5176\u4e2d\u50ac\u5316\u53d1\u5939\u7ec4\u4ef6\uff08CHA\uff09\u5df2\u88ab\u8bc1\u660e\u662f\u5176\u4e2d\u7684\u4e00\u79cd\u3002\u8fd9\u4e9b\u65e0\u9176\u7b49\u6e29CHA\u7535\u8def\u5177\u6709\u5feb\u901f\u6709\u6548\u7684\u4fe1\u53f7\u653e\u5927\u3001\u80cc\u666f\u4f4e\u3001\u5468\u8f6c\u7387\u9ad8\u7684\u4f18\u70b9\u3002\u57fa\u4e8e\u8fd9\u4e9b\u9ad8\u5ea6\u7684\u654f\u611f\u6027\u548c\u9776\u5411\u7279\u5f02\u6027CHA\u53cd\u5e94\u7684DNA\u7535\u8def\u5df2\u7ecf\u5e94\u7528\u4e8e\u5404\u79cd\u4f53\u5916\u65e0\u7ec6\u80de\u5206\u6790\uff0c\u5305\u62ec\u6838\u9178\u3001\u5c0f\u5206\u5b50\u548c\u86cb\u767d\u8d28\u7684\u68c0\u6d4b\u548c\u5b9a\u91cf\u5206\u6790\u3002\u4f46\u662f\u5927\u591a\u6570\u57fa\u4e8eDNA\u7684\u7535\u8def\u7684\u7ec6\u80de\u5185\u529f\u80fd\u5b58\u5728\u751f\u7269\u9012\u9001\u548c\u964d\u89e3\u56f0\u96be\u7684\u7f3a\u70b9\u3002\u800c\u53e6\u4e00\u65b9\u9762\uff0cRNA\u5206\u5b50\u662f\u53ef\u4ee5\u5728\u751f\u547d\u7cfb\u7edf\u5185\u8fdb\u884c\u9057\u4f20\u7f16\u7801\u548c\u8f6c\u5f55\u7684\u3002\u56e0\u6b64\uff0c\u57fa\u4e8eRNA\u7684\u7535\u8def\u548c\u88c5\u7f6e\u5728\u7ec6\u80de\u5185\u5e94\u7528\u5e94\u8be5\u662f\u5177\u6709\u5f88\u5927\u7684\u6f5c\u529b\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5927\u5b66\u7684\u5c24\u660e\u65ed\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u7b49\u62a5\u9053\u4e86\u4e00\u79cd\u57fa\u4e8eRNA\u7684\u50ac\u5316\u53d1\u5939\u7ec4\u88c5\u7535\u8def\u7684\u9057\u4f20\u7f16\u7801\uff0c\u7528\u4e8e\u6d3b\u7ec6\u80de\u5185\u7684\u7075\u654fRNA\u6210\u50cf\u3002 \u8367\u5149RNA\u9002\u4f53Broccoli\u7684\u5206\u88c2\u5f62\u5f0f\u7528\u4f5c\u62a5\u9053\u5206\u5b50\u3002 \u4e00\u79cd\u9776RNA\u53ef\u4ee5\u50ac\u5316\u5730\u89e6\u53d1\u6570\u5341\u81f3\u6570\u767e\u4e2aBroccoli\u7684\u8367\u5149\uff0c\u53ef\u4ee5\u7075\u654f\u5730\u9776\u5411\u7684\u68c0\u6d4bRNA\u3002\u5e76\u4e14\u8fdb\u4e00\u6b65\u4f18\u5316\u4ed6\u4eec\u8bbe\u8ba1\u7684\u7535\u8def\uff0c\u53ef\u4ee5\u8f7b\u677e\u7684\u7f16\u7a0b\u4ee5\u4fbf\u9776\u5411\u5404\u79cdRNA\u5e8f\u5217\u8fdb\u884c\u6210\u50cf\u3002 \u8be5\u8bbe\u8ba1\u539f\u7406\u4e3a\u5f00\u53d1\u7528\u4e8e\u7ec6\u80de\u5e94\u7528\u7684\u5404\u79cd\u57fa\u56e0\u7f16\u7801RNA\u7535\u8def\u5f00\u8f9f\u4e86\u65b0\u7684\u821e\u53f0\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a\u201cGenetically Encoded Catalytic Hairpin Assembly for Sensitive RNA Imaging in Live Cells\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520aJACS<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe\u4e00\u3001CHARGE \u7684\u7535\u8def\u539f\u7406\u56fe\u00a0<\/strong>\"\"<\/strong><\/p>\n

\u56fe\u4e8c\u3001\u4e0d\u540c\u7684\u5e8f\u5217\u8bbe\u8ba1\u4f18\u5316CHARGE\u7684\u4fe1\u566a\u6bd4\u00a0\u00a0<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

\u56fe\u4e09\u3001D2 CHARGE\u7cfb\u7edf\u7684\u4f53\u5916\u8868\u5f81\u00a0<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

\uff08a\uff09 \u6709\u6216\u65e0\u9776\u6807\u4e0b\uff0cBroccoli\u548cCHARGE\u7535\u8def\u7684\u8367\u5149\u53d1\u5c04\u5149\u8c31\uff1b<\/p>\n

\uff08b\uff09 D2 CHARGE\u5bf9\u4e0d\u540c\u6d53\u5ea6\u9776\u6807\u7684\u8367\u5149\u54cd\u5e94\uff1b<\/p>\n

\uff08c\uff09 \u52a0\u5165\u4e0d\u540c\u91cf\u7684\u76ee\u6807\u65f6CHARGE\u7535\u8def\u7684\u52a8\u529b\u5b66\u56fe\uff1b<\/p>\n

\uff08d\uff09 Mg2 +<\/sup>\u6d53\u5ea6\u5bf9CHARGE\u7535\u8def\u7684\u5f71\u54cd\u3002<\/p>\n

\u56fe\u56db\u3001\u6d3bBL21\uff08DE3\uff09*\u7ec6\u80de\u8fdb\u884c\u5171\u805a\u7126\u8367\u5149\u6210\u50cf\u00a0<\/strong><\/p>\n

\"\"<\/strong>
\n \u56fe\u4e94\u3001\u6a21\u5757\u5316CHARGE\u7cfb\u7edf\u53ef\u68c0\u6d4b\u5404\u79cdRNA\u9776\u6807\u00a0<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

\uff08a\uff09 \u57fa\u4e8e\u5206\u5b50\u4fe1\u6807\u7684\u76ee\u6807\u68c0\u6d4b\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08b\uff09 \u7528\u5206\u5b50\u4fe1\u6807\u7ed3\u5408\u7684D2 CHARGE\u4f53\u5916\u68c0\u6d4bmiR21\u548cSgrS;<\/p>\n

\uff08c\uff09 \u6dfb\u52a0\u4e0d\u540c\u91cf\u8461\u8404\u7cd6\u53ca\u5176\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\u5206\u5e03\u540e\uff0c\u8868\u8fbeH1 + H2 + MBSgrs\u7684\u7ec6\u80de\u7684\u5171\u805a\u7126\u8367\u5149\u6210\u50cf\u56fe\u3002<\/p>\n

\u56fe\u516d\u3001\u57fa\u4e8eHHR\u7cfb\u7edf\u7684\u8336\u78b1\u8c03\u8282\u7ec6\u80deRNA\u6c34\u5e73\u00a0<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

\uff08a\uff09 \u8336\u78b1\u8bf1\u5bfc\u7684HHR\u81ea\u5207\u5272\u548c\u9776\u6807RNA\u91ca\u653e\u7684\u793a\u610f\u56fe;<\/p>\n

\uff08b\uff09 \u4e0d\u540c\u6d53\u5ea6\u7684\u8336\u78b1\u7684\u4f53\u5916\u8367\u5149\u6d4b\u5b9a\uff1b<\/p>\n

\uff08c\uff09 \u52a0\u5165\u4e0d\u540c\u6d53\u5ea6\u7684\u8336\u78b12 h\u540e\uff0c10-base-long\u6291\u5236\u5242\u63ba\u5165\u7684HHR\u7cfb\u7edf\u768410\uff05\u53d8\u6027PAGE\u51dd\u80f6\u8868\u5f81\uff1b<\/p>\n

\uff08d\uff09 \u4e0d\u540c\u6d53\u5ea6\u7684\u8336\u78b1\u5904\u7406\u7684\u8336\u78b1-HHR\u6784\u5efa\u4f53\u7684\u5171\u805a\u7126\u8367\u5149\u6210\u50cf\u56fe\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u7814\u7a76\u5f15\u5165\u4e86\u4e00\u79cd\u65b0\u578b\u7684\u57fa\u56e0\u7f16\u7801RNA\u7535\u8def\uff0c\u53ef\u4ee5\u8fdb\u884c\u9ad8\u7075\u654f\u5ea6\u5730\u68c0\u6d4b\u7ec6\u80deRNA\u9776\u6807\u3002 \u8fd9\u4e9bCHARGE\u7535\u8def\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u7f16\u7a0b\uff0c\u4ee5\u5bf9\u6d3b\u7ec6\u80de\u4e2d\u7684\u5404\u79cdRNA\u9776\u6807\u8fdb\u884c\u6210\u50cf\u3002\u5373\u4f7f\u5728\u7ec6\u83cc\u7ec6\u80de\u4e2d\uff0c\u5f53\u524d\u7684\u7535\u8def\u4e5f\u5f97\u5230\u8bc1\u5b9e\uff0cCHARGE\u786e\u5b9e\u4e5f\u5177\u6709\u771f\u6838\u7ec6\u80de\u7814\u7a76\u7684\u6f5c\u529b\u3002\u7c7b\u4f3c\u4e8e\u7528\u4e8e\u4f53\u5916\u5206\u6790\u7684\u50ac\u5316\u53d1\u5939\u7ec4\u88c5\u7535\u8def\u7684\u901a\u7528\u5e94\u7528\u5e73\u53f0\uff0c\u53ef\u4ee5\u5f00\u53d1\u51fa\u5404\u79cdCHARGE\u4ee5\u8fdb\u4e00\u6b65\u68c0\u6d4b\u5176\u4ed6\u7ec6\u80deRNA\u3001\u86cb\u767d\u8d28\u548c\u5c0f\u5206\u5b50\uff0c\u4ee5\u53ca\u7528\u4e8e\u9057\u4f20\u8c03\u8282\u7684\u5f00\u5173\u3002 \u8be5\u7814\u7a76\u4e3a\u5229\u7528\u9057\u4f20\u7f16\u7801\u7684RNA\u7535\u8def\u5f00\u53d1\u7ec6\u80de\u5e94\u7528\u5f00\u8f9f\u4e86\u65b0\u7684\u65b9\u6cd5\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aGenetically Encoded Catalytic Hairpin Assembly for Sensitive RNA Imaging in Live Cells<\/a><\/strong>\u00a0\uff08JACS, 2018<\/strong>, DOI: 10.1021\/jacs.8b03956\uff09<\/p>\n

\u5c24\u660e\u65ed\u6559\u6388\u56e2\u961f\u4e8e2016\u5e749\u6708\u6210\u7acb\uff0c\u76ee\u524d\u7531\u4e24\u540d\u535a\u58eb\u540e\u548c4\u540d\u535a\u58eb\u751f\u7ec4\u6210\u3002\u8fd9\u4e9b\u8367\u5149RNA\u5206\u5b50\u662f\u5728\u535a\u58eb\u540e\u5bfc\u5e08Samie Jaffrey\u7ec4\u91cc\u9996\u5148\u53d1\u73b0\u7684\u3002\u76f8\u5173\u6587\u732e\u5305\u62ec\uff1a<\/strong><\/p>\n

http:\/\/science.sciencemag.org\/content\/333\/6042\/642.full<\/a><\/p>\n

http:\/\/science.sciencemag.org\/content\/335\/6073\/1194.long<\/a><\/p>\n

http:\/\/www.pnas.org\/content\/112\/21\/E2756.long<\/a><\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u7ec4\u5c0f\u80d6\u7eb8\u7f16\u8bd1\u3002<\/strong><\/p>\n

\u6750\u6599\u725b\u7f51\u662f\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/strong><\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com.<\/p>\n

\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaokefu.<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a><\/strong>\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 DNA\u548cRNA\u6298\u53e0\u6210\u7cbe\u786e\u7eb3\u7c73\u7ed3\u6784\u7684\u80fd\u529b\u5df2\u88ab\u7528\u4e8e\u5404\u79cd\u751f\u7269\u5b66\u548c\u751f\u7269\u533b\u5b66\u9886\u57df\u3002\u8fd9\u4e9b\u6838\u9178\u7cbe\u786e\u81ea\u7ec4\u88c5\u7684\u7eb3\u7c73\u7ed3\u6784\u4e3a\u53ef\u7f16\u7a0b\u5206\u5b50\u8bc6\u522b\u5de5\u5177\u7684\u5f00\u53d1\u521b\u9020\u4e86\u5f88\u597d\u7684\u5e73\u53f0\u3002\u5728\u8fc7\u53bb…<\/p>\n","protected":false},"author":7770,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215,2],"tags":[3416,3415],"class_list":["post-146193","post","type-post","status-publish","format-standard","hentry","category-jiedu","category-keji","tag-charge","tag-rna"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/146193"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/7770"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=146193"}],"version-history":[{"count":7,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/146193\/revisions"}],"predecessor-version":[{"id":146244,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/146193\/revisions\/146244"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=146193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=146193"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=146193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}