{"id":154667,"date":"2018-10-03T06:50:21","date_gmt":"2018-10-02T22:50:21","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=154667"},"modified":"2018-10-01T20:50:41","modified_gmt":"2018-10-01T12:50:41","slug":"%e9%ba%bb%e7%9c%81%e7%90%86%e5%b7%a5%e5%ad%a6%e9%99%a2nano-lett-%ef%bc%9a%e4%bc%98%e5%8c%96%e5%8f%af%e9%99%8d%e8%a7%a3%e8%81%9a%e5%90%88%e7%89%a9-%e8%84%82%e8%b4%a8%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=154667","title":{"rendered":"\u9ebb\u7701\u7406\u5de5\u5b66\u9662Nano Lett.\uff1a\u4f18\u5316\u53ef\u964d\u89e3\u805a\u5408\u7269-\u8102\u8d28\u7eb3\u7c73\u7c92\u5b50\u7528\u4e8emRNA\u6709\u6548\u7cfb\u7edf\u9012\u9001\u5230\u80ba\u5185\u76ae\u7ec6\u80de\u548c\u80ba\u514d\u75ab\u7ec6\u80de"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u4f53\u5916\u8f6c\u5f55mRNA\u5408\u6210\u7684\u7814\u7a76\u8fdb\u5c55\u5f15\u53d1\u4e86\u7814\u7a76\u8005\u4eec\u5bf9\u901a\u8fc7mRNA\u9012\u9001\u6765\u5b9e\u73b0\u591a\u79cd\u75be\u75c5\u6cbb\u7597\u7684\u7814\u7a76\u5174\u8da3\u3002\u4ece\u5728\u4f53\u5185\u53ef\u63a7\u4ea7\u751f\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u89d2\u5ea6\u6765\u8bf4\uff0cmRNA\u80fd\u591f\u77ac\u65f6\u8868\u8fbe\uff0c\u800c\u4e14\u8fd8\u80fd\u6d88\u9664\u57fa\u56e0\u7ec4\u63d2\u5165\u7684\u98ce\u9669\uff0c\u4e0eDNA\u76f8\u6bd4\u5177\u6709\u663e\u8457\u7684\u4f18\u52bf\u3002\u800c\u6cbb\u7597\u6027mRNA\u9012\u9001\u4ecd\u7136\u9700\u8981\u7ed5\u8fc7\u8bf8\u591a\u969c\u788d\uff0c\u6bd4\u5982RNase\u4ecb\u5bfc\u7684\u964d\u89e3\u3001\u8fdb\u5165\u7ec6\u80de\u4ee5\u53ca\u5185\u4f53\u9003\u9038\u7b49\u3002\u76ee\u524d\u5df2\u7ecf\u5f00\u53d1\u51fa\u591a\u79cd\u8f7d\u4f53\u80fd\u591f\u5c06\u6838\u9178\u9012\u9001\u5230\u9776\u7ec6\u80de\u4e2d\uff0c\u5176\u4e2d\u65e0\u6bd2\u7eb3\u7c73\u7c92\u5b50\u662f\u4e00\u79cd\u91cd\u8981\u7684mRNA\u9012\u9001\u8f7d\u4f53\uff0c\u5df2\u7ecf\u5728\u514d\u75ab\u7597\u6cd5\u3001\u86cb\u767d\u8d28\u66ff\u4ee3\u548c\u57fa\u56e0\u7f16\u8f91\u7b49\u65b9\u9762\u5f97\u5230\u5e94\u7528\uff0c\u4f46\u662f\u4e3b\u8981\u7684\u7814\u7a76\u5de5\u4f5c\u8fd8\u76f8\u5bf9\u5355\u4e00\u5730\u96c6\u4e2d\u5728\u5bf9\u4e8e\u809d\u810f\u90e8\u4f4d\u7684\u9012\u9001\u3002\u56e0\u6b64\uff0c\u8981\u5b9e\u73b0\u4e34\u5e8a\u4e0a\u5e7f\u6cdb\u7684RNA\u6cbb\u7597\uff0c\u5c31\u9700\u8981\u5f00\u53d1\u80fd\u591f\u6709\u6548\u4e14\u7279\u5f02\u6027\u5730\u9012\u9001mRNA\u5230\u975e\u809d\u810f\u5668\u5b98\u7684\u9012\u9001\u8f7d\u4f53\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u7f8e\u56fd\u9ebb\u7701\u7406\u5de5\u5b66\u9662<\/strong>Daniel G. Anderson<\/strong>\u6559\u6388<\/strong>\u5229\u7528\u53ef\u964d\u89e3\u7684\u805a\uff08\u03b2-\u6c28\u57fa\u916f\uff09\u4e0e\u805a\uff08\u4e59\u4e8c\u9187\uff09-\u8102\u8d28\u7f00\u5408\u7269\u914d\u5236\u6210\u7eb3\u7c73\u7c92\u5b50\uff0c\u5e76\u901a\u8fc7\u5b9e\u9a8c\u8bbe\u8ba1\u8bc1\u5b9e\u8fd9\u79cd\u53ef\u964d\u89e3\u805a\u5408\u7269-\u8102\u8d28\u7eb3\u7c73\u7c92\u5b50\u80fd\u591f\u5728\u805a\u5408\u7269\u7684\u5408\u6210\u548c\u7eb3\u7c73\u7c92\u5b50\u914d\u5236\u65b9\u9762\u8fdb\u884c\u4f18\u5316\uff0c\u4ece\u800c\u5b9e\u73b0mRNA\u9012\u9001\u6548\u7387\u591a\u4e2a\u6570\u91cf\u7ea7\u7684\u589e\u52a0\u3002\u6b64\u5916\uff0c\u7814\u7a76\u8fd8\u8868\u660emRNA\u53ef\u4ee5\u88ab\u9012\u9001\u5230\u80ba\u5185\u76ae\u7ec6\u80de\u548c\u80ba\u514d\u75ab\u7ec6\u80de\u4e2d\uff0c\u8fdb\u4e00\u6b65\u6269\u5c55\u4e86\u8fd9\u4e9b\u7eb3\u7c73\u7c92\u5b50\u7684\u6f5c\u5728\u6548\u7528\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a”Optimization of a Degradable Polymer-Lipid Nanoparticle for Potent Systemic Delivery of mRNA to the Lung Endothelium and Immune Cells”<\/strong>\u53d1\u8868\u5728Nano Letters<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe<\/strong>1 <\/strong>\u7528\u4e8e\u4f53\u5185<\/strong>mRNA<\/strong>\u9012\u9001\u5355\u4f53\u548c\u914d\u65b9\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u57fa\u4e8e\u5148\u524d\u62a5\u9053\u7684\u7528\u4e8ePABE\u4e09\u5143\u5171\u805a\u7269\u5408\u6210\u7b5b\u9009\u7684\u5355\u4f53\uff1b<\/p>\n

(b) \u63cf\u8ff0\u7528\u4e8e\u4f53\u5185mRNA\u9012\u9001\u7684\u914d\u65b9\u7b5b\u9009\u4e2d\u7684\u914d\u65b9\u90e8\u5206\u7684\u793a\u610f\u56fe\u3002<\/p>\n

\u56fe2 \u90e8\u5206\u56e0\u5b50\u7b5b\u9009\u6709\u6548\u7684\u805a\u5408\u7269<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u5728Hela\u7ec6\u80de\u4e2d\u8fdb\u884c\u4f53\u5916\u9012\u9001\u65f6\uff0c\u7528\u4e8e\u4f18\u5316PBAE\u5408\u6210\u53c2\u6570\u7684\u90e8\u5206\u56e0\u5b50\u7b5b\u9009\u4f18\u5316\u63ed\u793a\u4e86\u51e0\u79cd\u66f4\u52a0\u6709\u6548\u7684\u805a\u5408\u7269\uff1b<\/p>\n

(b) A1\u805a\u5408\u7269\u7684\u7ed3\u6784\u7279\u5f81\u3002<\/p>\n

\u56fe3 \u7f16\u7801\u8367\u5149\u7d20\u9176\u7684mRNA\u5728\u5c0f\u9f20\u4e2d\u9759\u8109\u5185\u9012\u9001\u53ca24\u5c0f\u65f6\u5404\u5668\u5b98\u53d1\u5149\u8bc4\u4f30<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u901a\u8fc7\u660e\u786e\u7684\u7b5b\u9009\u63ed\u793a\u4e86\u4e00\u4e2a\u6bd4\u57fa\u7840\u914d\u65b9\u66f4\u52a0\u6709\u6548\u7684\u914d\u65b9\uff1b<\/p>\n

(b) \u90e8\u5206\u56e0\u5b50\u7b5b\u9009\u5728\u80ba\u90e8\u5177\u6709\u66f4\u591a\u6709\u6548\u7684\u914d\u65b9\uff0c\u800c\u5728\u813e\u4e2d\u51e0\u79cd\u5236\u5242\u663e\u793a\u51fa\u9ad8\u8367\u5149\u7d20\u9176\u4fe1\u53f7\uff1b<\/p>\n

(c) \u901a\u8fc7\u4f18\u5316\u5236\u5242\u4e2dPEG-\u8102\u8d28\u7684\u6469\u5c14\u767e\u5206\u6bd4\u53ef\u4ee5\u5b9e\u73b0\u80ba\u90e8\u7684\u9ad8\u7279\u5f02\u6027\uff1b<\/p>\n

(d) \u4f18\u5316\u7684PBAE\u805a\u5408\u7269\/\u5236\u5242\uff08A1-L3\uff09\u5728\u591a\u79cdmRNA\u5242\u91cf\u4e0b\u6bd4jetPEI\u66f4\u6709\u6548\u3002<\/p>\n

\u56fe<\/strong>4\u00a0<\/strong>\u4f7f\u7528<\/strong>Ai14 Cre \/ lox<\/strong>\u62a5\u544a\u5c0f\u9f20\u8f6c\u67d3\u7684\u80ba\u7ec6\u80de\u7c7b\u578b\u7684\u5206\u6790<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u7ec6\u80de\u7c7b\u578b\u7684\u767e\u5206\u6bd4\u4e3aTdTomato +\uff08\u6761\u5f62\u56fe\uff0c\u5de6\u8f74\uff09\uff0c\u8868\u660e\u4f7f\u7528A1-L3\u7eb3\u7c73\u9897\u7c92\u6210\u529f\u8f6c\u67d3Cre mRNA\uff1b<\/p>\n

(b) \u5728Ai14\u5c0f\u9f20\u4e2d\u901a\u8fc7A1-L3\u7eb3\u7c73\u9897\u7c92\u9012\u9001Cre mRNA\u540e\u8868\u8fbetdTomato\u7684\u514d\u75ab\u7ec6\u80de\uff08CD45 +\uff09\u4e9a\u578b\u7684\u9274\u5b9a\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u672c\u6587\u4e2d\u5229\u7528\u4e00\u7cfb\u5217\u5b9e\u9a8c\u6765\u5b9e\u73b0\u5728\u805a\u5408\u7269\u5408\u6210\u4ee5\u53ca\u7eb3\u7c73\u7c92\u5b50\u914d\u5236\u65b9\u9762\u5bf9\u53ef\u964d\u89e3\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u7684\u4f18\u5316\uff0c\u5e76\u6210\u529f\u5f00\u53d1\u51fa\u4e00\u79cd\u5728\u4f53\u5916\u548c\u4f53\u5185\u5b9e\u9a8c\u4e2d\u9ad8\u51fa\u4e24\u4e2a\u6570\u91cf\u7ea7\u7684\u805a\u5408\u7269\u914d\u65b9\u3002\u8fd9\u4e9b\u5b9e\u9a8c\u65b9\u6cd5\u8bbe\u8ba1\u5728\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u4e0a\u7684\u4f7f\u7528\u8fdb\u4e00\u6b65\u8bc1\u660e\u4e86\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u7528\u4e8e\u4f18\u5316\u4f53\u5185mRNA\u9012\u9001\u8f7d\u4f53\u7684\u5de8\u5927\u6f5c\u529b\u3002\u6b64\u5916\uff0c\u672c\u6587\u6240\u6d89\u53ca\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u601d\u8def\u4e5f\u53ef\u4ee5\u5e94\u7528\u5230\u9488\u5bf9\u5176\u4ed6\u5668\u5b98mRNA\u9012\u9001\u7684\u7eb3\u7c73\u7c92\u5b50\u4f18\u5316\u4e2d\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aOptimization of a Degradable Polymer-Lipid Nanoparticle for Potent Systemic Delivery of mRNA to the Lung Endothelium and Immune Cells\u00a0<\/a>\u00a0<\/strong>(Adv. Mater. 2018, DOI: 10.1021\/acs.nanolett.8b02917)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u5b66\u672f\u7ec4biotech\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u5ba1\u6838\u6574\u7406\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenkefu\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u4f53\u5916\u8f6c\u5f55mRNA\u5408\u6210\u7684\u7814\u7a76\u8fdb\u5c55\u5f15\u53d1\u4e86\u7814\u7a76\u8005\u4eec\u5bf9\u901a\u8fc7mRNA\u9012\u9001\u6765\u5b9e\u73b0\u591a\u79cd\u75be\u75c5\u6cbb\u7597\u7684\u7814\u7a76\u5174\u8da3\u3002\u4ece\u5728\u4f53\u5185\u53ef\u63a7\u4ea7\u751f\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u89d2\u5ea6\u6765\u8bf4\uff0cmRNA\u80fd\u591f\u77ac\u65f6\u8868\u8fbe…<\/p>\n","protected":false},"author":7746,"featured_media":154668,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[3591,2518,2839],"class_list":["post-154667","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-mrna","tag-2518","tag-2839"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/154667"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/7746"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=154667"}],"version-history":[{"count":3,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/154667\/revisions"}],"predecessor-version":[{"id":155363,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/154667\/revisions\/155363"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/media\/154668"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=154667"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=154667"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=154667"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}