{"id":167014,"date":"2019-02-12T06:39:39","date_gmt":"2019-02-11T22:39:39","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=167014"},"modified":"2019-02-16T11:51:27","modified_gmt":"2019-02-16T03:51:27","slug":"%e6%b8%85%e5%8d%8e%e7%8e%8b%e5%ae%8f%e4%bc%9f%e3%80%81%e9%99%88%e4%ba%9a%e6%a5%a0%e5%8c%97%e5%a4%a7%e5%bd%ad%e6%b5%b7%e7%90%b3jacs%ef%bc%9a%e7%94%9f%e7%89%a9%e6%b4%bb%e6%80%a7%e5%8a%9f%e8%83%bd","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=167014","title":{"rendered":"\u6e05\u534e\u738b\u5b8f\u4f1f\u3001\u9648\u4e9a\u6960&#038;\u5317\u5927\u5f6d\u6d77\u7433JACS\uff1a\u751f\u7269\u6d3b\u6027\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u52a9\u529b\u9ad8\u5206\u8fa8\u51b7\u51bb\u7535\u955c"},"content":{"rendered":"<p>\u8fd1\u5e74\u6765\uff0c\u51b7\u51bb\u7535\u5b50\u663e\u5fae\u955c\u6280\u672f\u5f97\u76ca\u4e8e\u786c\u4ef6\u548c\u8f6f\u4ef6\u7684\u6280\u672f\u9769\u65b0\uff0c\u53d1\u5c55\u8fc5\u731b\uff0c\u5df2\u7ecf\u6210\u4e3a\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u6280\u672f\u624b\u6bb5\u3002\u7136\u800c\u51b7\u51bb\u7535\u955c\u6280\u672f\u4ecd\u7136\u9762\u4e34\u7740\u4e00\u4e9b\u74f6\u9888\uff0c\u4e25\u91cd\u9650\u5236\u4e86\u8be5\u6280\u672f\u89e3\u6790\u751f\u7269\u5206\u5b50\u7ed3\u6784\u7684\u6548\u7387\u3002\u5176\u4e2d\u4e00\u4e2a\u91cd\u8981\u74f6\u9888\u662f\u51b7\u51bb\u6837\u54c1\u7684\u5236\u5907\u3002\u8be5\u8fc7\u7a0b\u9700\u8981\u5c06\u542b\u6709\u751f\u7269\u5927\u5206\u5b50\u7684\u6eb6\u6db2\u52a0\u5230\u7535\u955c\u8f7d\u7f51\u4e0a\uff0c\u7136\u540e\u8fc5\u901f\u51b7\u51bb\u5230\u6db2\u6001\u4e59\u70f7\u4e2d\uff0c\u5c06\u751f\u7269\u5927\u5206\u5b50\u77ac\u95f4\u51b7\u51bb\u5728\u4e00\u5c42\u5927\u7ea6\u51e0\u5341\u7eb3\u7c73\u539a\u7684\u73bb\u7483\u6001\u51b0\u5c42\u4e2d\u3002\u76ee\u524d\u901a\u7528\u7684\u6837\u54c1\u5236\u5907\u652f\u6491\u819c\u5bb9\u6613\u5bfc\u81f4\u751f\u7269\u5927\u5206\u5b50\u5438\u9644\u5230\u6c14\u6db2\u754c\u9762\uff0c\u4ece\u800c\u53d1\u751f\u5206\u5b50\u7ed3\u6784\u7684\u53d8\u6027\u6216\u8005\u4f18\u52bf\u53d6\u5411\u7b49\u95ee\u9898\uff0c\u9020\u6210\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u89e3\u6790\u7684\u56f0\u96be\u3002<\/p>\n<p>\u8fd1\u65e5\uff0c\u6765\u81ea\u6e05\u534e\u5927\u5b66\u751f\u547d\u5b66\u9662\u7684\u738b\u5b8f\u4f1f\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09\uff0c \u9ad8\u7cbe\u5c16\u4e2d\u5fc3\u5353\u8d8a\u5b66\u8005\u9648\u4e9a\u6960\u535a\u58eb\uff08\u901a\u8baf\u4f5c\u8005\uff09\uff0c\u5218\u6960\u535a\u58eb\u751f\uff08\u7b2c\u4e00\u4f5c\u8005\uff09\u4e0e\u5317\u4eac\u5927\u5b66\u5316\u5b66\u5206\u5b50\u5de5\u7a0b\u5b66\u9662\u7684\u5f6d\u6d77\u7433\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09\uff0c\u5f20\u91d1\u707f\u535a\u58eb\u751f\uff08\u5171\u540c\u4e00\u4f5c\uff09\u5408\u4f5c\u5728\u300aJournal of the American Chemical Society\u300b\uff08JACS\uff09\u4e0a\u53d1\u8868\u9898\u4e3a\u201cBioactive functionalized monolayer graphene for high resolution cryo-EM\u201d\u7684\u7814\u7a76\u8bba\u6587\u3002\u4f5c\u8005\u8bbe\u8ba1\u5f00\u53d1\u4e86\u4e00\u79cd\u65b0\u578b\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u7535\u955c\u652f\u6491\u819c\u7528\u4e8e\u51b7\u51bb\u7535\u955c\u9ad8\u5206\u8fa8\u7387\u4e09\u7ef4\u7ed3\u6784\u89e3\u6790\u3002\u77f3\u58a8\u70ef\u5177\u6709\u9ad8\u5f3a\u5ea6\u97e7\u5ea6\u3001\u5355\u539f\u5b50\u5c42\u8584\u3001\u5f3a\u5bfc\u7535\u5bfc\u70ed\u6027\u7b49\u4f18\u5f02\u7279\u8d28\uff0c\u662f\u4e00\u79cd\u7406\u60f3\u7684\u7535\u955c\u6837\u54c1\u652f\u6491\u819c\u3002\u4f5c\u8005\u5c06CVD\u751f\u957f\u7684\u5927\u5355\u6676\u77f3\u58a8\u70ef\uff08\u9762\u79ef\u8d85\u8fc710mm<sup>2<\/sup>\uff09\u5229\u7528\u65e0\u80f6\u8f6c\u79fb\u6cd5\u8f6c\u79fb\u5230\u7535\u955c\u91d1\u5c5e\u8f7d\u7f51\u4e0a\uff0c\u5e76\u7ecf\u8fc7\u9ad8\u9530\u9178\u94be\u7b49\u5f3a\u6c27\u5316\u5242\u5904\u7406\uff0c\u6d3b\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\u8868\u9762\uff0c\u7136\u540e\u5171\u4ef7\u4fee\u9970\u4e0aNi-NTA\u7b49\u751f\u7269\u5b66\u79d1\u7814\u4e2d\u5e38\u7528\u7684\u86cb\u767d\u7eaf\u5316\u914d\u4f53\u3002\u8fd9\u79cd\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\u5177\u6709\u9ad8\u4eb2\u6c34\u6027\uff08\u4eb2\u6c34\u89d2\u8fbe\u523029\u00b0\uff09\u3002\u7ecf\u8fc7\u62c9\u66fc\u5149\u8c31\u548c\u7535\u5b50\u884d\u5c04\u7b49\u8868\u5f81\u53d1\u73b0\uff0c\u5316\u5b66\u4fee\u9970\u8fc7\u7a0b\u5bf9\u5355\u6676\u77f3\u58a8\u70ef\u7684\u6676\u4f53\u7ed3\u6784\u3001\u6297\u7535\u5b50\u8f90\u7167\u635f\u4f24\u80fd\u529b\u7b49\u5f71\u54cd\u4e0d\u5927\uff0c\u6781\u5927\u5730\u4fdd\u6301\u4e86\u77f3\u58a8\u70ef\u539f\u6709\u7684\u4f18\u5f02\u7279\u8d28\u3002\u5728\u8367\u5149\u663e\u5fae\u955c\u548c\u8d1f\u67d3\u7535\u955c\u4e0b\uff0cNi-NTA\u4fee\u9970\u7684\u77f3\u58a8\u70ef\u5bf9\u5e26\u6709His\u6807\u7b7e\u7684\u7ea2\u8272\u8367\u5149\u86cb\u767d\uff08RFP\uff09\u548c\u6838\u82f7\u9178\u78f7\u9178\u5316\u9176\uff08PNPase\uff09\u5c55\u73b0\u4e86\u6781\u5f3a\u7684\u4eb2\u548c\u529b\uff0c\u80fd\u591f\u7279\u5f02\u6027\u5730\u5c06\u76ee\u6807\u86cb\u767d\u6355\u83b7\u3002\u4f5c\u8005\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e86\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u5bf9\u76ee\u6807\u86cb\u767d\u7684\u9009\u62e9\u6027\uff0c\u53d1\u73b0\u8fd9\u79cdNi-NTA\u4fee\u9970\u7684\u652f\u6491\u819c\u80fd\u591f\u4ece\u86cb\u767d\u9176\u4f53\u548c\u6838\u7cd6\u4f53\u7684\u6df7\u5408\u6eb6\u6db2\u4e2d\uff0c\u9009\u62e9\u6027\u5730\u6293\u53d6\u5e26\u6709His\u6807\u7b7e\u7684\u86cb\u767d\u9176\u4f53\uff0c\u800c\u5bf9\u4e0d\u542bHis\u6807\u7b7e\u7684\u6838\u7cd6\u4f53\u8d77\u5230\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u7684\u8fc7\u6ee4\u4f5c\u7528\u3002\u51b7\u51bb\u7535\u5b50\u65ad\u5c42\u626b\u63cf\uff08Cryo-ET\uff09\u8868\u660e\uff0c\u76ee\u6807\u86cb\u767d(\u86cb\u767d\u9176\u4f53)\u80fd\u591f\u7d27\u7d27\u7ed3\u5408\u5728\u751f\u7269\u6d3b\u6027\u914d\u4f53\u4fee\u9970\u7684\u77f3\u58a8\u70ef\u8868\u9762\uff0c\u8fdc\u79bb\u6c14\u6db2\u754c\u9762\uff0c\u4ece\u800c\u907f\u514d\u4e86\u86cb\u767d\u53d8\u6027\u7b49\u4f20\u7edf\u51b7\u51bb\u6837\u54c1\u5236\u5907\u65b9\u6cd5\u4e2d\u5e38\u89c1\u95ee\u9898\u3002\u4f5c\u8005\u5229\u7528\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\u5236\u5907\u51b7\u51bb\u6837\u54c1\uff0c\u5728Thermo Fisher Tecnai Arctica\u7535\u5b50\u663e\u5fae\u955c (200 keV\uff0c\u914d\u5907Falcon II\u76f4\u63a5\u7535\u5b50\u63a2\u6d4b\u76f8\u673a)\u4e0a\u6536\u96c6\u4e86\u51b7\u51bb\u7535\u955c\u6570\u636e\uff0c\u5e76\u6210\u529f\u89e3\u6790\u83b7\u5f97\u4e86\u86cb\u767d\u9176\u4f53\u7684\u8fd1\u539f\u5b50\u5206\u8fa8\u7387\uff083.8\u57c3\uff09\u7684\u4e09\u7ef4\u91cd\u6784\u3002<\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p>\u56fe1\uff1a\u751f\u7269\u6d3b\u6027\u914d\u4f53\u529f\u80fd\u5316\u4fee\u9970\u77f3\u58a8\u70ef\u7528\u4e8e\u51b7\u51bb\u7535\u955c\u9ad8\u5206\u8fa8\u7ed3\u6784\u89e3\u6790\u6d41\u7a0b\u793a\u610f\u56fe\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/1-5.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167015\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/1-5.png\" alt=\"\" width=\"600\" height=\"420\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/1-5.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/1-5-300x210.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>\u56fe2: \u5927\u5355\u6676\u77f3\u58a8\u70ef\u7535\u955c\u8f7d\u7f51Ni-NTA\u914d\u4f53\u5316\u5b66\u4fee\u9970\u8868\u5f81\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/2-4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167016\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/2-4.png\" alt=\"\" width=\"600\" height=\"577\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/2-4.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/2-4-300x289.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>\u56fe3: \u529f\u80fd\u5316\u4fee\u9970\u540e\u7684\u77f3\u58a8\u70ef\u5177\u6709\u9ad8\u4eb2\u6c34\u6027\uff0c\u5e76\u4e14\u57fa\u672c\u4fdd\u6301\u4e86\u77f3\u58a8\u70ef\u539f\u6709\u7684\u6676\u683c\u7ed3\u6784\u548c\u6297\u8f90\u7167\u635f\u4f24\u7684\u7279\u6027\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/3-4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167017\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/3-4.png\" alt=\"\" width=\"600\" height=\"384\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/3-4.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/3-4-300x192.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>\u56fe4: \u529f\u80fd\u5316\u4fee\u9970\u7684\u77f3\u58a8\u70ef\u652f\u6491\u819c\u5bf9\u76ee\u6807\u86cb\u767d\u8d28\u5206\u5b50\u5177\u6709\u5f3a\u7279\u5f02\u4eb2\u548c\u6027\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/4-2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167018\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/4-2.png\" alt=\"\" width=\"600\" height=\"485\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/4-2.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/4-2-300x243.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>\u56fe5: \u529f\u80fd\u5316\u77f3\u58a8\u70ef\u5bf9\u76ee\u6807\u86cb\u767d\u5177\u6709\u9009\u62e9\u7ed3\u5408\u6027\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/5-2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167019\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/5-2.png\" alt=\"\" width=\"600\" height=\"565\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/5-2.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/5-2-300x283.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>\u56fe6: \u529f\u80fd\u5316\u77f3\u58a8\u70ef\u80fd\u591f\u5c06\u76ee\u6807\u86cb\u767d\u951a\u5b9a\u5728\u652f\u6491\u819c\u8868\u9762\uff0c\u6709\u52a9\u4e8e\u83b7\u5f97\u8fd1\u539f\u5b50\u5206\u8fa8\u7387\u7684\u51b7\u51bb\u7535\u955c\u4e09\u7ef4\u91cd\u6784\u3002<\/p>\n<p><a href=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/6.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-167020\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/6.png\" alt=\"\" width=\"600\" height=\"599\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/6.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/6-150x150.png 150w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/02\/6-300x300.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" 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functionalized monolayer graphene for high resolution 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