{"id":175071,"date":"2019-05-12T06:07:54","date_gmt":"2019-05-11T22:07:54","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=175071"},"modified":"2019-05-11T11:08:41","modified_gmt":"2019-05-11T03:08:41","slug":"%e5%8d%97%e5%bc%80%e5%a4%a7%e5%ad%a6%e6%9d%a8%e5%bf%97%e8%b0%8b%e5%9b%a2%e9%98%9f-adv-mater-%e7%bb%bc%e8%bf%b0-%e5%a4%9a%e8%82%bd%e7%9a%84%e9%85%b6%e4%bf%83%e8%87%aa%e7%bb%84%e8%a3%85eisa%e5%92%8c","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=175071","title":{"rendered":"\u5357\u5f00\u5927\u5b66\u6768\u5fd7\u8c0b\u56e2\u961f Adv. Mater.\u7efc\u8ff0: \u591a\u80bd\u7684\u9176\u4fc3\u81ea\u7ec4\u88c5(EISA)\u548c\u51dd\u80f6\u5316"},"content":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011<\/strong><\/p>\n

\u4f17\u6240\u5468\u77e5\uff0c\u81ea\u7ec4\u88c5\u662f\u751f\u7269\u7cfb\u7edf\u4e2d\u666e\u904d\u5b58\u5728\u7684\u4e00\u79cd\u73b0\u8c61\uff0c\u5176\u4e3b\u8981\u662f\u901a\u8fc7\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u4f7f\u65e0\u5e8f\u7684\u5355\u4e2a\u5206\u5b50\u81ea\u53d1\u5f62\u6210\u6709\u5e8f\u7684\u8d85\u5206\u5b50\u7ed3\u6784\u7684\u8fc7\u7a0b\u3002\u56e0\u6b64\uff0c\u81ea\u7ec4\u88c5\u53ef\u4ee5\u4f7f\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u5177\u6709\u591a\u79cd\u529f\u80fd\uff0c\u662f\u4e00\u79cd\u5236\u9020\u590d\u6742\u751f\u7269\u6750\u6599\u7684\u901a\u7528\u5e73\u53f0\uff0c\u5728\u751f\u7269\u533b\u5b66\u548c\u751f\u7269\u6750\u6599\u9886\u57df\u6709\u5f88\u5927\u7684\u5e94\u7528\u4ef7\u503c\u3002\u8d85\u5206\u5b50\u6750\u6599\u7684\u6027\u8d28\u4e0e\u5176\u5206\u5b50\u6784\u7b51\u5355\u5143\u548c\u5236\u5907\u65b9\u6cd5\u5bc6\u5207\u76f8\u5173\u3002\u5728\u4f17\u591a\u7684\u6784\u7b51\u5355\u5143\u4e2d\uff0c\u77ed\u80bd\u7531\u4e8e\u5177\u6709\u826f\u597d\u7684\u751f\u7269\u6d3b\u6027\u548c\u751f\u7269\u76f8\u5bb9\u6027\u800c\u5177\u5907\u5f88\u5f3a\u7684\u53d1\u5c55\u6f5c\u529b\u77ed\u80bd\u672c\u8eab\u5177\u6709\u4f4e\u7a33\u5b9a\u6027\u548c\u5728\u4f53\u5185\u6613\u88ab\u9176\u964d\u89e3\u7684\u7f3a\u70b9\uff0c\u963b\u788d\u4e86\u5176\u8fdb\u4e00\u6b65\u8f6c\u5316\u4e3a\u836f\u7269\u6216\u8bca\u65ad\u5de5\u5177\u3002\u77ed\u80bd\u81ea\u7ec4\u88c5\u6210\u7684\u8d85\u5206\u5b50\u6750\u6599\uff0c\u80fd\u591f\u5ef6\u957f\u5176\u4f53\u5185\u534a\u8870\u671f\u548c\u63d0\u9ad8\u5176\u751f\u7269\u6d3b\u6027\u3002\u56e0\u6b64\uff0c\u81ea\u7ec4\u88c5\u591a\u80bd\u4e0d\u4ec5\u5728\u836f\u7269\u9012\u9001\u6750\u6599\u3001\u514d\u75ab\u4f50\u5242\u548c\u7ec4\u7ec7\u5de5\u7a0b\u652f\u67b6\u7b49\u9886\u57df\u6709\u5de8\u5927\u7684\u5e94\u7528\u524d\u666f\uff0c\u540c\u65f6\uff0c\u5bf9\u5176\u7ec4\u88c5\u884c\u4e3a\u7684\u7814\u7a76\u6709\u52a9\u4e8e\u6211\u4eec\u7406\u89e3\u751f\u547d\u7cfb\u7edf\u4e2d\u7684\u5929\u7136\u81ea\u7ec4\u88c5\u7ed3\u6784\u6216\u75be\u75c5\u76f8\u5173\u7684\u86cb\u767d\u5f02\u5e38\u7ec4\u88c5\u884c\u4e3a\u3002<\/p>\n

\u76ee\u524d\u5df2\u7ecf\u6709\u8bb8\u591a\u7b56\u7565\u6765\u89e6\u53d1\u80bd\u7684\u81ea\u7ec4\u88c5\u548c\u5f62\u6210\u8d85\u5206\u5b50\u6750\u6599\uff0c\u4f8b\u5982\u6e29\u5ea6\u53d8\u5316\u3001pH\u8c03\u8282\u3001\u79bb\u5b50\u5f3a\u5ea6\u53d8\u5316\u7b49\u7b49\uff0c\u4f46\u662f\u5927\u591a\u6570\u7b56\u7565\u53ea\u80fd\u5728\u79bb\u4f53\u7f13\u51b2\u6eb6\u6db2\u4e2d\u4f7f\u7528\uff0c\u4e0e\u4f53\u5185\u73af\u5883\u4e0d\u540c\uff0c\u963b\u788d\u4e86\u5176\u5728\u751f\u7269\u533b\u5b66\u9886\u57df\u7684\u5e94\u7528\u3002\u6700\u8fd1\uff0c\u9176\u4fc3\u81ea\u7ec4\u88c5\uff08EISA\uff09\u7b56\u7565\u6210\u4e3a\u5728\u4f53\u5185\u73af\u5883\u4e2d\u8fdb\u884c\u539f\u4f4d\u81ea\u7ec4\u88c5\u7684\u6709\u6548\u65b9\u6cd5\u3002\u76ee\u524dEISA\u7b56\u7565\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u764c\u7ec6\u80de\u6291\u5236\u3001\u7ec4\u7ec7\u5de5\u7a0b\u4ee5\u53ca\u836f\u7269\u8f93\u9001\u7b49\u9886\u57df\u3002\u6b64\u5916\uff0c\u81ea\u7ec4\u88c5\u7684\u52a8\u529b\u5b66\u548c\u70ed\u529b\u5b66\u9014\u5f84\u5bf9\u4e8e\u81ea\u7ec4\u88c5\u80bd\u7684\u5f62\u8c8c\u548c\u529f\u80fd\u662f\u81f3\u5173\u91cd\u8981\u7684\u3002\u9176\u50ac\u5316\u5728\u52a8\u529b\u5b66\u4e0a\u662f\u53ef\u63a7\u7684\uff0c\u5b83\u80fd\u591f\u63d0\u4f9b\u66f4\u7cbe\u786e\u4e14\u53ef\u63a7\u7684\u6784\u5efa\u591a\u80bd\u7c7b\u8d85\u5206\u5b50\u6750\u6599\u7684\u65b9\u6cd5\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u5357\u5f00\u5927\u5b66\u7684\u6768\u5fd7\u8c0b\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09\u56e2\u961f<\/strong>\u62a5\u9053\u4e86\u5173\u4e8e\u9176\u4fc3\u81ea\u7ec4\u88c5\uff08EISA\uff09\u5728\u80bd\u5236\u5907\u5177\u6709\u751f\u7269\u529f\u80fd\u7684\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u548c\u6c34\u51dd\u80f6\u65b9\u9762\u7684\u5e94\u7528\u3002\u672c\u6587\u4e2d\u4f5c\u8005\u91cd\u70b9\u5173\u6ce8EISA\u7684\u72ec\u7279\u6027\u8d28\uff0c\u4ee5\u5236\u5907\u5176\u4ed6\u5e38\u89c4\u65b9\u6cd5\u65e0\u6cd5\u83b7\u5f97\u7684\u8d85\u5206\u5b50\u6750\u6599\u3002\u9996\u5148\uff0c\u4f5c\u8005\u8ba8\u8bba\u4e86\u7528\u4e8e\u6d3b\u7ec6\u80de\u4e2d\u6210\u50cf\u6216\u6cbb\u7597\u7684\u591a\u80bd\u539f\u4f4dEISA\u3002\u63a5\u7740\uff0c\u4f5c\u8005\u63a2\u8ba8\u4e86\u5728\u7531EISA\u5f62\u6210\u7684\u8d85\u5206\u5b50\u6750\u6599\u7684\u6027\u8d28\u4e2d\u524d\u4f53\u7684\u91cd\u8981\u6027\u3002\u540c\u65f6\uff0c\u4f5c\u8005\u8fd8\u4ecb\u7ecd\u4e86\u901a\u8fc7EISA\u4f7f\u591a\u80bd\u53ef\u63a7\u6298\u53e0\u548c\u81ea\u7ec4\u88c5\u7684\u7b56\u7565\u3002\u6700\u540e\uff0c\u4f5c\u8005\u5f3a\u8c03\u901a\u8fc7\u4e0e\u5176\u5b83\u65b9\u6cd5\u7684\u7ed3\u5408\uff0cEISA\u53ef\u4ee5\u4ee5\u66f4\u7cbe\u786e\u7684\u6a21\u5f0f\u548c\u65f6\u7a7a\u63a7\u5236\u5236\u5907\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u3002\u672c\u6587\u7740\u91cd\u5f3a\u8c03\u4e86EISA\u5bf9\u591a\u80bd\u81ea\u7ec4\u88c5\u7684\u91cd\u8981\u6027\uff0c\u6709\u52a9\u4e8e\u8fdb\u4e00\u6b65\u63a8\u52a8\u57fa\u4e8e\u591a\u80bd\u7684\u6750\u6599\u7684\u4e34\u5e8a\u8f6c\u5316\u3002\u8be5\u7efc\u8ff0\u4ee5\u9898\u4e3a\u201cEnzyme-Instructed Self-Assembly (EISA) and Hydrogelation of Peptides\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520aAdv. Mater.<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/strong><\/p>\n

1\u3001EISA\u539f\u4f4d\u5f62\u6210\u8d85\u5206\u5b50\u6750\u6599<\/strong><\/p>\n

\u5728\u751f\u7406\u6761\u4ef6\u4e0b\uff0c\u9176\u53ef\u4ee5\u6709\u6548\u5730\u50ac\u5316\u5f62\u6210\u548c\u8f6c\u5316\u8bb8\u591a\u751f\u7269\u5b66\u4e0a\u91cd\u8981\u7684\u5206\u5b50\u3002\u56e0\u6b64\uff0c\u5229\u7528\u9176\u4fc3\u8fdb\u5206\u5b50\u81ea\u7ec4\u88c5\u662f\u4e00\u79cd\u6709\u6548\u4e14\u6e29\u548c\u7684\u5236\u5907\u8d85\u5206\u5b50\u6750\u6599\u7684\u65b9\u6cd5\u3002\u6b64\u5916\uff0c\u9176\u7684\u8868\u8fbe\u6c34\u5e73\u4e0e\u75be\u75c5\u4e5f\u662f\u5bc6\u5207\u76f8\u5173\u3002\u7531\u4e8e\u75be\u75c5\u90e8\u4f4d\u5982\u80bf\u7624\u4e2d\u67d0\u4e9b\u9176\u7684\u8fc7\u91cf\u8868\u8fbe\uff0c\u4f7f\u5f97EISA\u53ef\u80fd\u53d1\u751f\u5728\u75be\u75c5\u90e8\u4f4d\u6216\u5f02\u5e38\u7ec6\u80de\u5185\u3002\u6240\u4ee5\uff0c\u901a\u8fc7EISA\u539f\u4f4d\u5f62\u6210\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u53ef\u4ee5\u7528\u4e8e\u75be\u75c5\u7684\u8bca\u65ad\u548c\u6cbb\u7597\u3002<\/p>\n

1.1\u3001\u764c\u7ec6\u80de\u4e2d\u78f7\u9178\u9176\u539f\u4f4d\u5f62\u6210\u8367\u5149\u7eb3\u7c73\u7ea4\u7ef4\u7528\u4e8e\u6210\u50cf<\/strong><\/p>\n

\u56fe\u4e00\u3001\u5229\u7528\u80bf\u7624\u4e2d\u8fc7\u8868\u8fbe\u7684\u78f7\u9178\u9176\u539f\u4f4d\u5f62\u6210\u7eb3\u7c73\u7ea4\u7ef4\u548c\u6c34\u51dd\u80f6<\/strong>
\n \"\"A\u3001\u901a\u8fc7\u78f7\u9178\u9176\u50ac\u5316\u7684\u53bb\u78f7\u9178\u5316\u5f62\u6210\u7684\u524d\u4f53\u53ca\u5176\u76f8\u5e94\u7684\u8367\u5149\u6c34\u51dd\u80f6\u5316\u5408\u7269\u7684\u5316\u5b66\u7ed3\u6784\uff1b<\/p>\n

B\u3001\u5728\u7ec6\u80de\u5185\u6210\u50cf\u9176\u89e6\u53d1\u7684\u8d85\u5206\u5b50\u81ea\u7ec4\u88c5\u7684\u6210\u50cf\u539f\u7406\uff1b<\/p>\n

C\u3001\u8367\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u56fe\u50cf\uff1b<\/p>\n

D\u3001\u901a\u8fc7\u5728PBS\u4e2d\u6df7\u5408100 \u03bcL\u6bcf\u79cd\u7a00\u91ca\u7684\u7ec6\u80de\u5206\u6570\u548c\u5316\u5408\u72691\uff0c\u6bcf\u79cd\u6837\u54c1\u7684\u5149\u5b66\u56fe\u50cf\u3002<\/p>\n

1.2\u3001\u57fa\u8d28\u91d1\u5c5e\u86cb\u767d\u9176\uff08MMP\uff09\u539f\u4f4d\u5f62\u6210\u7eb3\u7c73\u7ea4\u7ef4\u6291\u5236\u764c\u7ec6\u80de<\/strong><\/p>\n

\u56fe\u4e8c\u3001\u5bf9\u80bf\u7624\u4e2d\u8fc7\u91cf\u8868\u8fbe\u7684\u57fa\u8d28\u91d1\u5c5e\u86cb\u767d\u9176-7 (MMP -7)\u7684\u5229\u7528<\/strong>
\n \"\"A\u3001MMP-7\u50ac\u5316\u7684\u81ea\u7ec4\u88c5\u548c\u524d\u4f53\uff08ER-C16\uff09\u53ca\u5176\u76f8\u5e94\u7684\u51dd\u80f6\u5242\uff08G-C16\uff09\u7684\u5316\u5b66\u7ed3\u6784\uff1b<\/p>\n

B-E\u3001\u7528ER-C16\uff08B, C\uff09\u6216G-C16\uff08D, E\uff09\u5b75\u80b218 h\u540e\uff0c\u5bf9HeLa\u7ec6\u80de\uff08B, D\uff09\u548cMvE\u7ec6\u80de\uff08C, E\uff09\u7684\u6d3b\/\u6b7b\u7387\u6d4b\u5b9a\u3002<\/p>\n

1.3\u3001\u5728\u7ebf\u7c92\u4f53\u4e2d\u539f\u4f4d\u5f62\u6210\u7eb3\u7c73\u7ea4\u7ef4\u4ee5\u6291\u5236\u764c\u7ec6\u80de<\/strong><\/p>\n

\u56fe\u4e09\u3001\u63a5\u6709\u6291\u5236\u5242\u7684\u9776\u5411\u5206\u5b50\u7528\u4e8e\u7ebf\u7c92\u4f53EISA<\/strong>
\n \"\"A\u3001\u524d\u4f53\uff08L-1P\u548cD-1P\uff09\u53ca\u5176\u76f8\u5e94\u7684\u6210\u80f6\u5206\u5b50\uff08L-1\u548cD-1\uff09\u7684\u5316\u5b66\u7ed3\u6784\uff0c\u4ee5\u53ca\u7528\u4e8e\u9776\u5411\u7ebf\u7c92\u4f53\u548c\u8bf1\u5bfc\u764c\u7ec6\u80de\u6b7b\u4ea1\u7684EISA\u673a\u7406\uff1b<\/p>\n

B\u3001\u7528L-1P\u6216D-1P\uff0850\u00d710-6<\/sup> M\uff09\u5904\u74064 h\u7684Saos2\u7ec6\u80de\u7684\u5171\u805a\u7126\u6fc0\u5149\u626b\u63cf\u663e\u5fae\u955c\u56fe\u50cf\uff0c\u7528Mito-\u5b9a\u4f4d\u67d3\u6599\u67d3\u8272\u3002<\/p>\n

2\u3001\u524d\u4f53\u53c2\u4e0eEISA<\/strong><\/p>\n

EISA\u662f\u4e00\u79cd\u6bd4\u4f20\u7edf\u65b9\u6cd5\u66f4\u590d\u6742\u7684\u5206\u5b50\u81ea\u7ec4\u88c5\u89e6\u53d1\u65b9\u6cd5\u3002\u5176\u6210\u80f6\u524d\u4f53\u53ef\u80fd\u5728\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u7684\u5f62\u6210\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002\u5728EISA\u8fc7\u7a0b\u4e2d\uff0c\u524d\u4f53\u53ef\u4ee5\u4e0e\u751f\u6210\u7684\u5206\u5b50\u534f\u540c\u7ec4\u88c5\uff0c\u4ece\u800c\u7a33\u5b9a\u5e76\u4fc3\u8fdb\u7eb3\u7c73\u7ed3\u6784\u7684\u5f62\u6210\u3002\u901a\u8fc7\u4f18\u5316EISA\u8fc7\u7a0b\u7684\u6761\u4ef6\uff0c\u5305\u62ec\u9176\u6d53\u5ea6\u548c\u6e29\u5ea6\u53d8\u5316\u4ee5\u53ca\u4e0d\u540c\u524d\u4f53\u7684\u4f7f\u7528\uff0c\u53ef\u4ee5\u5f62\u6210\u4e0d\u540c\u7684\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u3002\u4f8b\u5982\uff0c\u5f53\u4f7f\u7528\u78f7\u9178\u5316\u7684\u524d\u4f53\u548c\u4f4e\u6d53\u5ea6\u7684\u9176\u65f6\uff0c\u758f\u6c34\u6027\u80bd\u53ea\u80fd\u901a\u8fc7EISA\u5f62\u6210\u6c34\u51dd\u80f6\u3002<\/p>\n

2.1\u3001\u5229\u7528EISA\u5bf9\u758f\u6c34\u6027\u80bd\u8fdb\u884c\u51dd\u80f6\u5316<\/strong><\/p>\n

\u56fe\u56db\u3001\u751f\u7269\u73af\u5883\u4e2d\u758f\u6c34\u5316\u5408\u7269\u5f62\u6210\u7684\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599<\/strong>
\n \"\"A\u3001\u524d\u4f534\u53ca\u5176\u76f8\u5e94\u7684\u6210\u80f6\u5206\u5b505\u7684\u5316\u5b66\u7ed3\u6784\u548c\u524d\u4f53\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

B\u30014 \u7684 PBS\u7f13\u51b2\u6db2\uff08pH 8.0\uff09\u6eb6\u6db2\uff1b<\/p>\n

C\u3001\u5728PBS\u7f13\u51b2\u6db2\uff08pH 8.0\uff09\u4e2d\u5bf95\u8d85\u58f0\u5904\u7406\u540e\uff1b<\/p>\n

D\u3001\u901a\u8fc7\u54114\u7684\u6eb6\u6db2\u4e2d\u52a0\u5165\u78f7\u9178\u9176\u5f62\u6210\u7684\u60ac\u6d6e\u6db2\uff1b<\/p>\n

E\u3001\u901a\u8fc7\u5c06\u78f7\u9178\u9176\u52a0\u5165\u52304\u7684\u6eb6\u6db2\u4e2d\u5f62\u6210\u6c34\u51dd\u80f6I\uff1b<\/p>\n

F\u3001\u901a\u8fc7\u54114\u7684\u6eb6\u6db2\u4e2d\u52a0\u5165\u78f7\u9178\u9176\u5f62\u6210\u7684\u6c34\u51dd\u80f6II\uff1b<\/p>\n

G, H\u3001\u5728pH\u4e3a0\u548c9.0\u7684\u6c34\u6eb6\u6db2\u4e2d\u7684\u6c34\u51dd\u80f6II\u3002<\/p>\n

2.2\u3001\u901a\u8fc7\u524d\u4f53\u7684\u9884\u7ec4\u88c5\u7f29\u5c0f\u8d85\u5206\u5b50\u7ec4\u88c5\u4f53\u7684\u591a\u6837\u6027<\/strong><\/p>\n

\u56fe\u4e94\u3001\u5177\u6709\u4e0d\u540c\u78f7\u9178\u5316\u4f4d\u70b9\u7684\u524d\u4f53\u7684EISA\u8fc7\u7a0b<\/strong>
\n \"\"A\u3001\u901a\u8fc7\u78f7\u9178\u9176\u4ecepY1<\/sub>\u3001pY2<\/sub>\u548cpY3<\/sub>\u8f6c\u5316\u7684\u524d\u4f53pY1<\/sub>\u3001pY2<\/sub>\u3001pY3<\/sub>\u548cNap-YYY\u7684\u5316\u5b66\u7ed3\u6784\uff1b<\/p>\n

B\u3001pY1<\/sub>\u3001pY2<\/sub>\u548cpY3<\/sub>\u6eb6\u6db2\u7684CD\u5149\u8c31\uff1b<\/p>\n

C\u3001pY1<\/sub>\u3001pY2<\/sub>\u548cpY3<\/sub>\u6eb6\u6db2\u7684\u6fc0\u5149\u7167\u5c04\u56fe\u50cf\uff08\u6709\u5149\u8def\u8bf4\u660e\u6709\u81ea\u7ec4\u88c5\u4f53\u5f62\u6210\uff09\uff1b<\/p>\n

D\u3001\u4e09\u79cd\u524d\u4f53\u7684\u4e0d\u540c\u81ea\u7ec4\u88c5\u884c\u4e3a\u7684\u793a\u610f\u56fe\u3002<\/p>\n

2.3\u3001\u9176\u63a7\u5236\u80bd\u7ec4\u88c5\u7684\u6784\u8c61<\/strong><\/p>\n

\u56fe\u516d\u3001\u4e09\u79cd\u4e0d\u540c\u78f7\u9178\u5316\u4f4d\u70b9\u7684\u53d1\u5939\u5f62\u6210\u80bd\u7684\u524d\u4f53<\/strong>
\n \"\"A\u3001PP1\u3001PP2\u548cPP3\u7684\u5316\u5b66\u7ed3\u6784\u548c\u53d1\u5939\u80bd\u7684EISA\u8fc7\u7a0b\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

B\u3001\u52a0\u5165LPP\u540ePP1\u7684\u65f6\u95f4\u4f9d\u8d56\u6027CD\u5149\u8c31\uff1b<\/p>\n

C\u3001\u6ca1\u6709\u6dfb\u52a0LPP\u7684PP2\u7684CD\u5149\u8c31\uff1b<\/p>\n

D\u3001\u4e0d\u6dfb\u52a0LPP\u7684PP3\u7684CD\u5149\u8c31\u3002<\/p>\n

3\u3001\u901a\u8fc7EISA\u63a7\u5236\u591a\u80bd\u6298\u53e0\u548c\u7ec4\u88c5<\/strong><\/p>\n

\u4f17\u6240\u5468\u77e5\uff0c\u591a\u80bd\u7684\u6784\u8c61\u5bf9\u7eb3\u7c73\u6750\u6599\u7684\u5f62\u6001\u548c\u751f\u7269\u6d3b\u6027\u5177\u6709\u91cd\u8981\u7684\u5f71\u54cd\u3002\u7136\u800c\uff0c\u76ee\u524d\u63a7\u5236\u591a\u80bd\u7684\u6298\u53e0\u4ecd\u7136\u5177\u6709\u6311\u6218\u6027\u3002\u5c3d\u7ba1\u591a\u80bd\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u7684\u5f62\u6001\u5177\u6709\u591a\u6837\u6027\uff0c\u4f46\u5176\u529f\u80fd\u5374\u96be\u4ee5\u4f18\u5316\u3002\u5229\u7528EISA\u4ece\u524d\u4f53\u4e2d\u4ea7\u751f\u81ea\u7ec4\u88c5\u591a\u80bd\uff0c\u7279\u522b\u662f\u5728\u4f4e\u6e29\u6761\u4ef6\u4e0b\u7684\u524d\u4f53\u591a\u80bd\u7684\u6784\u8c61\u66f4\u52a0\u5747\u5300\uff0c\u56e0\u6b64\u4ea7\u751f\u7684\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u80fd\u5728\u66f4\u9ad8\u7684\u80fd\u91cf\u6c34\u5e73\u4e0a\u88ab\u52a8\u529b\u5b66\u6355\u83b7\uff0c\u4ece\u800c\u4ea7\u751f\u5177\u6709\u6700\u4f73\u751f\u7269\u6d3b\u6027\u7684\u66f4\u6709\u5e8f\u7684\u7eb3\u7c73\u6750\u6599\u3002\u540c\u4e00\u591a\u80bd\u6bb5\u5728\u4e0d\u540c\u6e29\u5ea6\u4e0b\u901a\u8fc7\u6539\u53d8\u81ea\u7ec4\u88c5\u9014\u5f84\uff0c\u4f8b\u5982\u4ece\u52a0\u70ed\u3001\u51b7\u5374\u5230EISA\uff0c\u7531\u4e8e\u591a\u80bd\u6bb5\u7684\u6784\u8c61\u4e0d\u540c\uff0c\u53ef\u80fd\u4f1a\u81ea\u7ec4\u88c5\u6210\u5177\u6709\u4e0d\u540c\u5f62\u8c8c\u7684\u7eb3\u7c73\u6750\u6599\u3002\u56e0\u6b64\uff0c\u8fd9\u662f\u4e00\u79cd\u901a\u8fc7\u4f18\u5316\u591a\u80bd\u7684\u6784\u8c61\u548c\u8d85\u5206\u5b50\u7eb3\u7c73\u6750\u6599\u7684\u7406\u5316\u6027\u8d28\u800c\u5f97\u5230\u9884\u671f\u751f\u7269\u6d3b\u6027\u6750\u6599\u7684\u7b80\u5355\u800c\u6709\u6548\u7684\u7b56\u7565\u3002<\/p>\n

3.1\u3001\u901a\u8fc7EISA\u5f62\u6210\u03b1-\u87ba\u65cb<\/strong><\/p>\n

\u56fe\u4e03\u3001EISA\u5728\u8f85\u52a9\u80bd\u6298\u53e0\u548c\u4fc3\u8fdb\u7eb3\u7c73\u836f\u7269\u7684\u5f62\u6210\u7684\u4f5c\u7528<\/strong>
\n \"\"A\u30016\u548c7\u7684\u5316\u5b66\u7ed3\u6784\u4ee5\u53ca\u901a\u8fc7\u52a0\u70ed-\u51b7\u5374\u8fc7\u7a0b\u5f62\u6210\u76846\u7684\u60ac\u6d6e\u6db2\u548c\u7531\u78f7\u9178\u9176\u57284 \u2103\u50ac\u5316\u7684\u8d85\u5206\u5b50\u6c34\u51dd\u80f67\u7684\u5149\u5b66\u56fe\u50cf\uff1b<\/p>\n

B\u3001CD\u5149\u8c31\u663e\u793a\u901a\u8fc7EISA\u57284 \u2103\u5f62\u6210\u7684\u7eb3\u7c73\u7ea4\u7ef4\u4e2d\u80bd\u7684\u03b1-\u87ba\u65cb\u6784\u8c61\u548c\u901a\u8fc7\u52a0\u70ed-\u51b7\u5374\u8fc7\u7a0b\u5f62\u6210\u7684\u7eb3\u7c73\u9897\u7c92\u4e2d\u80bd\u7684\u03b2-\u6298\u53e0\u6784\u8c61\uff1b<\/p>\n

C\u3001CRB\u3001\u7eb3\u7c73\u7ea4\u7ef4\u548c\u7eb3\u7c73\u9897\u7c92\u5bf9\u4e0d\u540c\u764c\u7ec6\u80de\u7684IC50<\/sub>\u503c\uff1b<\/p>\n

D\u3001CRB\u3001\u7eb3\u7c73\u7ea4\u7ef4\u548c\u9488\u5bf94T1\u80bf\u7624\u6a21\u578b\u7684\u7eb3\u7c73\u9897\u7c92\u7684\u4f53\u5185\u6297\u764c\u6548\u7387\u3002<\/p>\n

3.2\u3001EISA\u5bf9\u80bd\u6298\u53e0\u7684\u52a8\u529b\u5b66\u63a7\u5236<\/strong><\/p>\n

\u56fe\u516b\u3001EISA\u5bf9\u80bd\u6298\u53e0\u7684\u52a8\u529b\u5b66\u7684\u5f71\u54cd<\/strong>
\n \"\"A\u3001\u524d\u4f538\u548c\u7ed3\u6784\u5355\u51439\u7684\u5316\u5b66\u7ed3\u6784\u4ee5\u53ca\u80bd\u5728\u4e0d\u540c\u6e29\u5ea6\u4e0b\u7684\u5206\u5b50\u81ea\u7ec4\u88c5\u7684\u6a21\u5f0f\uff1b<\/p>\n

B\u3001\u57284 \u2103\u4e0b\u5f62\u6210\u7684\u6c34\u51dd\u80f6\u53ca\u5176TEM\u56fe\u50cf\uff1b<\/p>\n

C\u3001\u572837 \u2103\u4e0b\u5f62\u6210\u7684\u6f84\u6e05\u6eb6\u6db2\u53ca\u5176TEM\u56fe\u50cf\uff1b<\/p>\n

D\u3001\u57284 \u2103\u4e0b\u5f62\u6210\u7684\u7eb3\u7c73\u7ea4\u7ef4\u548c37 \u2103\u4e0b\u5f62\u6210\u7684\u7eb3\u7c73\u9897\u7c92\u7684CD\u5149\u8c31\uff1b<\/p>\n

E\u3001\u5728\u4e0d\u540c\u65f6\u95f4\u70b9\u5bf9\u7eb3\u7c73\u7ea4\u7ef4\u548c\u7eb3\u7c73\u9897\u7c92\u7684\u7ec6\u80de\u6444\u53d6\u3002<\/p>\n

4\u3001EISA\u4e0e\u5176\u4ed6\u65b9\u6cd5\u7684\u7ed3\u5408<\/strong><\/p>\n

\u867d\u7136\u8bf8\u5982\u80bf\u7624\u7b49\u75be\u75c5\u90e8\u4f4d\u7684\u67d0\u4e9b\u9176\u7684\u8868\u8fbe\u6c34\u5e73\u8f83\u9ad8\uff0c\u4f46\u8fd9\u4e9b\u9176\u5728\u6b63\u5e38\u7ec4\u7ec7\u6216\u6b63\u5e38\u7ec6\u80de\u4e2d\u4e5f\u6709\u4e00\u5b9a\u7684\u8868\u8fbe\u6c34\u5e73\u3002\u56e0\u6b64\uff0c\u5728\u75be\u75c5\u90e8\u4f4d\u9009\u62e9\u6027\u5f62\u6210\u8d85\u5206\u5b50\u7eb3\u7c73\u836f\u7269\u7684\u7a97\u53e3\u8303\u56f4\u975e\u5e38\u72ed\u7a84\u3002\u901a\u8fc7\u4e24\u79cd\u9176\u6216\u5176\u4ed6\u65b9\u6cd5\u4e0eEISA\u7ed3\u5408\uff0c\u53ef\u4ee5\u5728\u75c5\u7076\u90e8\u4f4d\u5f62\u6210\u66f4\u6709\u9009\u62e9\u6027\u3001\u66f4\u7cbe\u786e\u7684\u8d85\u5206\u5b50\u6750\u6599\u3002<\/p>\n

4.1\u3001\u7ec6\u80de\u73af\u5883\u5206\u5316\u7684\u5206\u5b50\u81ea\u7ec4\u88c5<\/strong><\/p>\n

\u56fe\u4e5d\u3001\u7ec6\u80de\u73af\u5883\u5206\u5316\u9009\u62e9\u6027\u5f62\u6210\u4e0d\u540c\u7684\u7eb3\u7c73\u836f\u7269<\/strong>
\n \"\"A\u300110\u7684\u5316\u5b66\u7ed3\u6784\u548c\u7ec6\u80de\u5916\u73af\u5883\u4e2dEISA\u7684\u793a\u610f\u56fe\u548c\u7ec6\u80de\u5185GSH\u63a7\u5236\u7684\u7f29\u5408\uff1b<\/p>\n

B\u3001\u6c34\u51dd\u80f611\u548c\u6c34\u51dd\u80f6\u4e2d\u7684\u7eb3\u7c73\u7ea4\u7ef4\uff1b<\/p>\n

C\u3001\u6c34\u51dd\u80f611-D\u548c\u6c34\u51dd\u80f6\u4e2d\u7684\u7eb3\u7c73\u7ea4\u7ef4\u3002<\/p>\n

4.2\u3001\u809d\u764c\u7ec6\u80de\u4e2d\u7684\u4e32\u8054\u5206\u5b50\u81ea\u7ec4\u88c5<\/strong><\/p>\n

\u56fe\u5341\u3001\u4e32\u8054\u5206\u5b50\u81ea\u7ec4\u88c5\u7b56\u7565\u5bf9\u809d\u764c\u7ec6\u80de\u7684\u4f5c\u7528\u6548\u679c<\/strong>
\n \"\"A\u3001\u524d\u4f5312\u548c\u76f8\u5e94\u7684\u7ed3\u6784\u5355\u514313\u548c14\u7684\u5316\u5b66\u7ed3\u6784\u4ee5\u53ca\u4e32\u8054\u81ea\u7ec4\u88c5\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

B, C\u3001\u5206\u522b\u4e0e12\uff08200\u00d710-3<\/sup> M\uff09\u5b75\u80b20.5\u548c2 h \u7684HepG2\u7ec6\u80de\u7684\u5171\u805a\u7126\u6fc0\u5149\u626b\u63cf\u663e\u5fae\u955c\u91cd\u5408\u56fe\u50cf\uff08\u84dd\u8272\u8367\u5149\uff1a\u7ec6\u80de\u6838\uff1b\u9ec4\u8272\u8367\u5149\uff1aNBD\u80bd\uff09\uff1b<\/p>\n

D\u3001\u5316\u5408\u726912, 13, 14\u548c15\u88ab\u764c\u75c7\u548c\u6b63\u5e38\u809d\u7ec6\u80de\u4e4b\u95f4\u7684\u7ec6\u80de\u6444\u53d6\u6bd4\u7387\uff1b<\/p>\n

E\u3001\u5316\u5408\u726912, 13, 14\u548c15\u5bf9\u764c\u75c7\u548c\u6b63\u5e38\u809d\u7ec6\u80de\u4e4b\u95f4\u7684\u7ec6\u80de\u6d3b\u529b\u6bd4\u7387\u3002<\/p>\n

5\u3001\u603b\u7ed3<\/strong><\/p>\n

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\u6587\u732e\u94fe\u63a5\uff1aEnzyme-Instructed Self-Assembly (EISA) and Hydrogelation of Peptides<\/a><\/strong>\uff08Adv. Mater., 2019<\/strong>, DOI: 10.1002\/adma.201805798\uff09<\/p>\n

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