{"id":175394,"date":"2019-05-16T06:30:03","date_gmt":"2019-05-15T22:30:03","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=175394"},"modified":"2019-05-15T20:14:50","modified_gmt":"2019-05-15T12:14:50","slug":"%e3%80%90%e5%ae%a1%e7%a8%bf%e5%b7%b2%e5%9b%9e%e5%a4%8d%e3%80%91%e6%b5%99%e6%b1%9f%e5%a4%a7%e5%ad%a6%e5%88%98%e5%bb%ba%e9%92%8a%e8%af%be%e9%a2%98%e7%bb%84%e5%92%8c%e5%94%90%e6%9c%ac%e5%bf%a0%e3%80%81","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=175394","title":{"rendered":"\u6d59\u6c5f\u5927\u5b66\u5218\u5efa\u948a\u8bfe\u9898\u7ec4\u548c\u5510\u672c\u5fe0\u3001\u5b59\u666f\u5fd7\u8bfe\u9898\u7ec4Anal. Chem.:\u8bca\u7597\u578bAIE\u63a2\u9488\u5728\u5bf9\u80bf\u7624\u8f85\u52a9\u5de8\u566c\u7ec6\u80de\u7684\u7279\u5f02\u6027\u8bc6\u522b\u3001\u6210\u50cf\u548c\u5149\u52a8\u529b\u5b66\u6cbb\u7597\u4e2d\u7684\u5e94\u7528"},"content":{"rendered":"

\u3010\u5f15\u8a00<\/strong>\u3011<\/p>\n

\u80bf\u7624\u7ec6\u80de\u5b58\u5728\u4e8e\u590d\u6742\u7684\u5fae\u73af\u5883\u4e2d\u3002\u8fd9\u79cd\u5fae\u73af\u5883\u5305\u542b\u8840\u7ba1\uff0c\u514d\u75ab\u7ec6\u80de\uff0c\u9aa8\u9ad3\u6765\u6e90\u7684\u708e\u75c7\u7ec6\u80de\uff0c\u6dcb\u5df4\u7ec6\u80de\uff0c\u4fe1\u53f7\u5206\u5b50\u548c\u7ec6\u80de\u5916\u57fa\u8d28\u3002\u5728\u80bf\u7624\u90e8\u4f4d\u7684\u514d\u75ab\u7ec6\u80de\u4e2d\uff0c\u5de8\u566c\u7ec6\u80de\u7279\u522b\u4e30\u5bcc\u3002\u4ed6\u4eec\u5b58\u5728\u4e8e\u80bf\u7624\u751f\u957f\u7684\u6240\u6709\u9636\u6bb5\u3002\u4e0e\u5176\u4ed6\u7ec6\u80de\u4e0d\u540c\uff0c\u5de8\u566c\u7ec6\u80de\u662f\u9ad8\u5ea6\u6781\u5316\u7684\uff0c\u5e76\u4e14\u53ef\u4ee5\u901a\u8fc7\u5c40\u90e8\u5fae\u73af\u5883\u523a\u6fc0\u4eceM0\u9636\u6bb5\u6781\u5316\u5230\u4e0d\u540c\u7684\u4e9a\u578b\uff0c\u5373M1\u548cM2\u3002M1\u548cM2\u5de8\u566c\u7ec6\u80de\u8868\u73b0\u51fa\u622a\u7136\u4e0d\u540c\u7684\u529f\u80fd\u3002\u7ecf\u5178\u7684M1\u5de8\u566c\u7ec6\u80de\u53ef\u4ee5\u589e\u5f3a\u514d\u75ab\u53cd\u5e94\u5e76\u6291\u5236\u80bf\u7624\u751f\u957f\uff1b\u800cM2\u5de8\u566c\u7ec6\u80de\u6291\u5236\u6297\u80bf\u7624\u514d\u75ab\u5e94\u7b54\u5e76\u4fc3\u8fdb\u80bf\u7624\u751f\u957f\u548c\u8840\u7ba1\u751f\u6210\uff0c\u79f0\u4e3a\u80bf\u7624\u8f85\u52a9\u5de8\u566c\u7ec6\u80de\uff08TAMs\uff09\u3002\u6709\u8d8a\u6765\u8d8a\u591a\u7684\u751f\u7269\u5b66\u7814\u7a76\u8bc1\u636e\u8868\u660eTAMs\u662f\u764c\u75c7\u6cbb\u7597\u7684\u9776\u70b9\u3002<\/p>\n

\u57fa\u4e8e\u4e4b\u524d\u7684\u7814\u7a76\uff0cTAMs\u7684\u7279\u5f02\u6027\u8bc6\u522b\u548c\u6d88\u9664\u662f\u63d0\u9ad8\u764c\u75c7\u6cbb\u7597\u6548\u7387\u7684\u6709\u6548\u65b9\u5f0f\u3002 \u76ee\u524d\u5bf9TAMs\u7684\u8bc6\u522b\u4e3b\u8981\u4f9d\u8d56\u4e8e\u4f20\u7edf\u7684\u751f\u5316\u624b\u6bb5\uff0c\u6bd4\u5982qRT-PCR\uff0c\u86cb\u767d\u8d28\u5370\u8ff9\u548c\u751f\u7269\u6807\u8bb0\u7269\u7684\u514d\u75ab\u8367\u5149\u67d3\u8272\u3002\u8fd9\u4e9b\u751f\u7269\u6280\u672f\u901a\u5e38\u4f9d\u8d56\u4e8e\u8017\u65f6\u7684\u7a0b\u5e8f\uff0c\u6602\u8d35\u7684\u4eea\u5668\u548c\u751f\u7269\u8bd5\u5242\uff0c\u9650\u5236\u4e86\u5176\u5728\u5feb\u901f\u4f53\u5185\u68c0\u6d4b\u4e2d\u7684\u5e94\u7528\u3002TAMs\u7684\u6cbb\u7597\u4e3b\u8981\u5229\u7528\u5316\u5b66\u836f\u7269\uff0c\u4f46\u662f\u5316\u5b66\u6cbb\u7597\u7684\u7597\u6548\u901a\u5e38\u53d7\u5230\u8010\u836f\u6027\u95ee\u9898\u7684\u9650\u5236\u3002\u6b64\u5916\uff0c\u6700\u8fd1\u7684\u7814\u7a76\u8fd8\u8868\u660e\uff0cTAMs\u8fd8\u4e0e\u764c\u7ec6\u80de\u7684\u6297\u836f\u6027\u76f8\u5173\u3002\u56e0\u6b64\u5316\u5b66\u7597\u6cd5\u4e0d\u662f\u6d88\u9664TAMs\u7684\u6709\u6548\u65b9\u6cd5\u3002\u56e0\u6b64\u9700\u8981\u5f00\u53d1\u7528\u4e8e\u8bc6\u522b\u548c\u6d88\u9664TAMs\u7684\u66ff\u4ee3\u65b9\u6cd5\u3002<\/p>\n

\u5149\u52a8\u529b\u7597\u6cd5\uff08PDT\uff09\u4f7f\u7528\u5149\u654f\u5242\u6765\u5b9e\u73b0\u5176\u6cbb\u7597\u76ee\u7684\uff0c\u5e76\u5177\u53ef\u4ee5\u6709\u6548\u514b\u670d\u8010\u836f\u6027\u95ee\u9898\u3002\u5149\u654f\u5242\u901a\u8fc7\u5149\u7167\u6d3b\u5316\u5e76\u4ea7\u751f\u6d3b\u6027\u6c27\uff08ROS\uff09\uff0c\u6740\u6b7b\u6076\u6027\u7ec6\u80de\u3002\u5927\u591a\u6570\u5149\u654f\u5242\u5177\u6709\u5149\u81f4\u53d1\u5149\u7279\u6027\uff0c\u80fd\u591f\u5728\u751f\u547d\u7cfb\u7edf\u4e2d\u4ee5\u9ad8\u5206\u8fa8\u7387\u8ddf\u8e2a\u7279\u6b8a\u7ec6\u80de\u6216\u7ec6\u80de\u8fc7\u7a0b\u3002\u539f\u5219\u4e0a\uff0c\u5149\u654f\u5242\u53ef\u4ee5\u8bbe\u8ba1\u6210\u540c\u65f6\u5177\u6709\u7ec6\u80de\u6210\u50cf\u548c\u6d88\u9664\u7684\u53cc\u91cd\u529f\u80fd\u3002\u7136\u800c\uff0c\u4f20\u7edf\u5149\u654f\u5242\u901a\u5e38\u5177\u6709\u5e73\u9762\u7ed3\u6784\uff0c\u503e\u5411\u4e8e\u901a\u8fc7\u03c0-\u03c0\u5806\u79ef\u5f62\u6210\u805a\u96c6\u4f53\u5e76\u4e14\u906d\u53d7\u805a\u96c6\u5f15\u8d77\u7684\u8367\u5149\u731d\u706d\uff08ACQ\uff09\u3002\u805a\u96c6\u4e0d\u4ec5\u5bfc\u81f4\u731d\u706d\u8367\u5149\uff0c\u8fd8\u5bfc\u81f4ROS\u4ea7\u7387\u964d\u4f4e\u3002 \u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e9b\u95ee\u9898\uff0c\u79d1\u7814\u5de5\u4f5c\u8005\u5df2\u7ecf\u505a\u51fa\u8bb8\u591a\u52aa\u529b\u6765\u89e3\u51b3ACQ\u5149\u654f\u5242\u7684\u805a\u96c6\u95ee\u9898\uff0c\u4f46\u662f\u6210\u679c\u5f88\u6709\u9650\u3002<\/p>\n

\u6700\u8fd1\uff0c\u5177\u6709\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\uff08AIE\uff09\u7279\u5f81\u7684\u8367\u5149\u56e2\u5f15\u8d77\u4e86\u5e7f\u6cdb\u7684\u7814\u7a76\u5173\u6ce8\u3002\u4e0eACQ\u67d3\u6599\u4e0d\u540c\u7684\u662f\uff0cAIE\u67d3\u6599\uff08AIEgens\uff09\u662f\u4e00\u7cfb\u5217\u5177\u6709\u87ba\u65cb\u6868\u5f62\u72b6\u7684\u5206\u5b50\u3002\u5f53AIE\u67d3\u6599\u4ee5\u5355\u5206\u5b50\u5f62\u5f0f\u6eb6\u89e3\u65f6\uff0c\u5b83\u4eec\u662f\u4e0d\u53d1\u5149\u7684\uff1b\u4f46\u5f53\u5176\u5f62\u6210\u805a\u96c6\u4f53\u65f6\uff0c\u7531\u4e8e\u5206\u5b50\u5185\u8fd0\u52a8\u53d7\u9650\uff08RIM\uff09\u800c\u5bfc\u81f4\u8367\u5149\u589e\u5f3a\u3002\u4e0eACQ\u67d3\u6599\u76f8\u6bd4\uff0cAIEgens\u901a\u5e38\u5177\u6709\u66f4\u5927\u7684\u65af\u6258\u514b\u65af\u4f4d\u79fb\uff0c\u5e76\u4e14\u53ef\u4ee5\u5bb9\u6613\u5730\u8bbe\u8ba1\u4e3a\u7528\u4e8e\u5177\u6709\u9ad8\u4fe1\u566a\u6bd4\u6210\u50cf\u7684\u70b9\u4eae\u578b\u63a2\u9488\u3002\u540c\u65f6\uff0cAIEgens\u6bd4ACQ\u67d3\u6599\u5177\u6709\u66f4\u597d\u7684\u8010\u5149\u6f02\u767d\u6027\uff0c\u56e0\u4e3a\u5b83\u4eec\u5728\u805a\u96c6\u72b6\u6001\u53ef\u4ee5\u5177\u6709\u66f4\u9ad8\u7684\u5de5\u4f5c\u6d53\u5ea6\u3002\u6b64\u5916\uff0cAIE\u5149\u654f\u5242\u5728\u805a\u96c6\u72b6\u6001\u4e0b\u663e\u793a\u51fa\u8f83\u9ad8\u7684ROS\u4ea7\u7387\uff0c\u5e76\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6210\u50cf\u8f85\u52a9\u7684\u5149\u52a8\u529b\u5b66\u6cbb\u7597\u4e2d\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb<\/strong>\u3011<\/p>\n

\u8fd1\u65e5\uff0c\u6d59\u6c5f\u5927\u5b66\u5218\u5efa\u948a\u6559\u6388\u8bfe\u9898\u7ec4\u3001\u5510\u672c\u5fe0\u9662\u58eb\u548c\u5b59\u666f\u5fd7\u6559\u6388\u8bfe\u9898\u7ec4\u3001\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u5218\u658c\u6559\u6388\u8bfe\u9898\u7ec4\u5408\u4f5c<\/strong>\u8bbe\u8ba1\u5e76\u5408\u6210\u4e86\u4e00\u79cd\u8bca\u7597\u578bAIE\u63a2\u9488\uff0c\u5373TPE-Man\uff0c\u5e94\u7528\u4e8eTAMs\u7684\u9776\u5411\u6210\u50cf\u548c\u5149\u52a8\u529b\u5b66\u6cbb\u7597\u4e2d\u3002TPE-Man\u7531\u5177\u6709\u7ea2\u5149\u53d1\u5c04\u7684AIE\u5149\u654f\u5242\u548c\u53ef\u4ee5\u7279\u5f02\u6027\u9776\u5411TAMs\u7684\u03b1-\u7518\u9732\u7cd6\u82f7\u7ec4\u6210\u3002TPE-Man\u663e\u793a\u51fa\u5bf9TAMs\u7684\u4f18\u5f02\u9776\u5411\u7279\u5f02\u6027\uff0c\u8fd9\u5f52\u56e0\u4e8e\u7518\u9732\u7cd6\u548cCD206\u4e4b\u95f4\u7684\u7279\u5f02\u6027\u76f8\u4e92\u4f5c\u7528\u3002\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790\u548c\u5171\u805a\u7126\u6fc0\u5149\u626b\u63cf\u663e\u5fae\u955c\uff08CLSM\uff09\u6210\u50cf\u786e\u5b9a\u6210\u50cf\u7684\u7279\u5f02\u6027\u3002\u6b64\u5916\uff0cTPE-Man\u5e94\u7528\u4e8e\u7ec4\u7ec7\u5207\u7247\u6210\u50cf\uff0c\u5e76\u663e\u793a\u4e0e\u5546\u4e1a\u6297\u4f53\u514d\u75ab\u67d3\u8272\u5177\u6709\u663e\u8457\u7684\u4fe1\u53f7\u91cd\u53e0\uff0c\u8868\u660e\u63a2\u9488\u5177\u6709\u75be\u75c5\u8bca\u65ad\u7684\u6f5c\u529b\u3002\u5728\u66b4\u9732\u4e8e\u53ef\u89c1\u5149\u7167\u65f6\uff0c\u7ec6\u80de\u5185\u7684TPE-Man\u4ea7\u751fROS\u53ef\u4ee5\u6709\u6548\u5730\u6740\u6b7bTAMs\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a\u201cSpecific Targeting, Imaging, and Ablation of Tumor-Associated Macrophages by Theranostic Mannose-AIEgen Conjugates<\/strong>\u201d\u53d1\u8868\u5728Anal. Chem.<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

Scheme 1.TPE-Man<\/strong>\u63a2\u9488\u5728\u5bf9\u80bf\u7624\u8f85\u52a9\u5de8\u566c\u7ec6\u80de\u7684\u7279\u5f02\u6027\u8bc6\u522b\u3001\u6210\u50cf\u548c\u5149\u52a8\u529b\u5b66\u6cbb\u7597\u4e2d\u7684\u5e94\u7528<\/strong><\/p>\n

\"\"<\/p>\n

Scheme 2.M0<\/strong>\u548c<\/strong>TAMs<\/strong>\u7684\u57f9\u80b2\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

Figure 1.<\/strong>\u6ef4\u5b9a\u5b9e\u9a8c<\/strong><\/p>\n

\"\"<\/p>\n

(a,d).TPE-Man\u548cTPE-Gal\u5206\u522b\u5bf9Con A\u548cPNA\u7684\u6d53\u5ea6\u54cd\u5e94<\/p>\n

(b,e).I\/I0<\/sub>-1\u968f\u51dd\u96c6\u7d20\u6d53\u5ea6\u7684\u53d8\u5316<\/p>\n

(c,f).TPE-Man\u548cTPE-Gal\u5bf9Con A\u548cPNA\u7684\u7279\u5f02\u6027<\/p>\n

Figure 2.TPE-Man<\/strong>\u5bf9<\/strong>TAMs<\/strong>\u7684\u7279\u5f02\u6027\u8bc6\u522b<\/strong><\/p>\n

\"\"<\/p>\n

(a).qRT-PCR\u5bf9M0\u548cTAMs\u4e2d\u6807\u8bb0\u7269\u542b\u91cf\u7684\u6d4b\u5b9a<\/p>\n

(b).TPE-Man\u548cTPE-Gal\u5206\u522b\u5bf9M0\u548cTAMs\u7684\u6210\u50cf<\/p>\n

(c).\u7528TPE-Man\u5206\u522b\u5904\u7406M0\u548cTAMs\u540e\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790<\/p>\n

Figure 3.TPE-Man<\/strong>\u5bf9<\/strong>TAMs<\/strong>\u4e0a<\/strong>CD206<\/strong>\u7684\u7279\u5f02\u6027\u8bc6\u522b<\/strong><\/p>\n

\"\"<\/p>\n

(a-c).\u4e0d\u540c\u6d53\u5ea6\u7518\u9732\u7cd6\u9884\u5904\u7406TAMs\u540e\u7684\u8367\u5149\u6210\u50cf<\/p>\n

(d-f).\u4e0d\u540c\u6d53\u5ea6\u7518\u9732\u7cd6\u9884\u5904\u7406M0\u540e\u7684\u8367\u5149\u6210\u50cf<\/p>\n

Figure 4.TPE-Man<\/strong>\u5bf9<\/strong>TAMs<\/strong>\u7684\u7279\u5f02\u6027\u8bc6\u522b<\/strong><\/p>\n

\"\"<\/p>\n

(a).TPE-Man\u7684\u5438\u6536\u548c\u53d1\u5c04\u5149\u8c31<\/p>\n

(b,c).TPE-Man\u548c\u5546\u4e1a\u67d3\u6599\u7684\u5149\u7a33\u5b9a\u6027\u5bf9\u6bd4<\/p>\n

Figure 5.TPE-Man<\/strong>\u4e0e\u514d\u75ab\u8367\u5149\u7684\u4fe1\u53f7\u5bf9\u6bd4<\/strong><\/p>\n

\"\"<\/p>\n

Figure 6.TPE-Man<\/strong>\u5bf9<\/strong>TAMs<\/strong>\u7684\u5149\u52a8\u529b\u5b66\u6cbb\u7597<\/strong><\/p>\n

\"\"<\/p>\n

(a).TAMs\u8d1f\u8f7d\u4e0d\u540c\u6d53\u5ea6\u7684TPE-Man\u7684\u7ec6\u80de\u5b58\u6d3b\u7387<\/p>\n

(b-d).TAMs\u8d1f\u8f7d\u4e0d\u540c\u6d53\u5ea6\u7684TPE-Man\u7684\u6d3b\u6b7b\u7ec6\u80de\u67d3\u8272<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

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Specific Targeting, Imaging, and Ablation of Tumor-Associated Macrophages by Theranostic Mannose-AIEgen Conjugates<\/a><\/p>\n

(Anal. Chem., 2019, DOI: 10.1021\/acs.analchem.9b01053)<\/p>\n

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