{"id":189612,"date":"2019-11-25T07:00:01","date_gmt":"2019-11-24T23:00:01","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=189612"},"modified":"2019-11-24T19:57:22","modified_gmt":"2019-11-24T11:57:22","slug":"%e7%ba%b3%e7%b1%b3%e6%9d%90%e6%96%99%e4%b8%9b%e6%9e%97%e4%b8%ad%e7%bf%a9%e7%bf%a9%e8%b5%b7%e8%88%9e%e7%9a%84%e5%9f%ba%e5%9b%a0%e9%ad%94%e5%89%aa","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=189612","title":{"rendered":"\u7eb3\u7c73\u6750\u6599\u4e1b\u6797\u4e2d\u7fe9\u7fe9\u8d77\u821e\u7684\u201c\u57fa\u56e0\u9b54\u526a\u201d"},"content":{"rendered":"<p>\u8fd1\u5e74\u6765\uff0c\u57fa\u4e8eCRISPR-Cas9\u7684\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5728\u6574\u4e2a\u79d1\u5b66\u9886\u57df\u90fd\u5f15\u8d77\u4e86\u4f17\u591a\u7814\u7a76\u5b66\u8005\u7684\u5174\u8da3\u3002\u4f5c\u4e3a\u4e00\u79cd\u57fa\u56e0\u526a\u8f91\u7cfb\u7edf\uff0c\u5b83\u53ef\u4ee5\u5bf9\u67d0\u4e00\u6838\u82f7\u9178\u5e8f\u5217\u4e2d\u7684\u7279\u5b9a\u57fa\u56e0\u4f4d\u70b9\u8fdb\u884c\u4eba\u4e3a\u6539\u53d8\uff0c\u63d2\u5165\u3001\u5220\u9664\u3001\u66ff\u6362\u6216\u4fee\u9970\u57fa\u56e0\u7ec4\u4e2d\u7684\u7279\u5b9a\u76ee\u7684\u57fa\u56e0\u4f7f\u5176\u8868\u8fbe\u6027\u72b6\u53d1\u751f\u6539\u53d8\u3002\u8fc4\u4eca\u4e3a\u6b62\uff0cCRISPR-Cas9\u5df2\u7ecf\u53ef\u4ee5\u7528\u4e8e\u591a\u79cd\u75be\u75c5\u7684\u6cbb\u7597\uff0c\u4f46\u662f\u5728\u4e34\u5e8a\u5e94\u7528\u5177\u6709\u53ef\u884c\u6027\u4e4b\u524d\uff0c\u4ecd\u7136\u5b58\u5728\u4e00\u4e9b\u6280\u672f\u969c\u788d\u3002\u7531\u4e8e\u5b83\u662f\u4e00\u79cd\u5927\u7684\u5206\u5b50\u590d\u5408\u7269\uff0c\u5f88\u96be\u5c06CRISPR\/Cas9\u76f4\u63a5\u9012\u9001\u5230\u7ec6\u80de\u6838\u4e2d\uff0c\u53ea\u6709\u5728\u7ec6\u80de\u6838\u4e2d\uff0c\u5b83\u624d\u80fd\u53d1\u6325\u5b83\u7684\u4f5c\u7528\uff1b\u540c\u65f6\u7531\u4e8eCRISPR\/Cas9\u76f4\u63a5\u5728\u57fa\u56e0\u5c42\u9762\u8fdb\u884c\u526a\u5207\uff0c\u5982\u4f55\u5c06\u5b83\u9776\u5411\u9012\u9001\u5230\u7279\u5b9a\u7684\u7ec4\u7ec7\u5668\u5b98\u4e5f\u662f\u4e00\u4e2a\u8f83\u5927\u7684\u96be\u9898\u3002\u56e0\u6b64\uff0c\u89e3\u51b3\u5176\u5728\u4e34\u5e8a\u5e94\u7528\u4e2d\u7684\u4f7f\u7528\u548c\u6548\u529b\u5c06\u80fd\u591f\u8fdb\u4e00\u6b65\u63a8\u52a8CRISPR\/Cas9\u7684\u53d1\u5c55\u3002<\/p>\n<p>\u76ee\u524d\uff0c\u5df2\u5b58\u5728\u4e00\u4e9b\u6280\u672f\u548c\u65b9\u6cd5\u6765\u5b9e\u73b0\u80de\u5185\u9012\u9001CRISPR\/Cas9\u590d\u5408\u7269\uff0c\u6bd4\u5982\u901a\u8fc7\u7535\u7a7f\u5b54\u53ef\u4ee5\u76f4\u63a5\u5c06CRISPR\/Cas9\u590d\u5408\u7269\u8f6c\u5bfc\u5230\u9776\u7ec6\u80de\u7684\u7ec6\u80de\u6838\uff1b\u6216\u8005\u901a\u8fc7\u75c5\u6bd2\u8f7d\u4f53\u4ecb\u5bfc\u7684\u65b9\u5f0f\u6765\u5b9e\u73b0\u4f20\u9012\u3002\u7136\u800c\u4f20\u7edf\u7684\u8fd9\u4e9b\u57fa\u56e0\u9012\u9001\u7684\u65b9\u5f0f\u4ecd\u7136\u5b58\u5728\u4e00\u4e9b\u6bd4\u5982\u5b89\u5168\u6027\u4f4e\u3001\u6548\u7387\u4e0d\u9ad8\u7684\u95ee\u9898\u3002\u56e0\u6b64\uff0c\u8fd1\u5e74\u6765\u6709\u5f88\u591a\u7814\u7a76\u91c7\u7528\u667a\u80fd\u578b\u7684\u7eb3\u7c73\u6750\u6599\u6765\u7a81\u7834\u4f20\u7edf\u8f7d\u4f53\u5bf9CRISPR\/Cas9\u590d\u5408\u7269\u9012\u9001\u7684\u5c40\u9650\u6027\u3002\u4e0b\u9762\uff0c\u5c31\u8ddf\u7b14\u8005\u6765\u4e00\u8d77\u770b\u770b\u8fd1\u51e0\u5e74\u6765\u57fa\u4e8eCRISPR-Cas9\u7684\u667a\u80fd\u578b\u7eb3\u7c73\u6750\u6599\u7684\u7814\u7a76\u8fdb\u5c55\u5427~<\/p>\n<p>\u7531\u4e8e\u4f20\u7edf\u75c5\u6bd2\u8f7d\u4f53\u5b58\u5728\u5f88\u5927\u7684\u5b89\u5168\u95ee\u9898\uff0c2016\u5e74\u5728<strong>ACS Nano<\/strong>\u4e0a\u9762\u62a5\u9053\u4e86\u6765\u81ea\u56db\u5ddd\u5927\u5b66\u534e\u897f\u533b\u9662\u751f\u7269\u6cbb\u7597\u4e0e\u764c\u75c7\u7814\u7a76\u4e2d\u5fc3\u56fd\u5bb6\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u7684\u4e00\u9879\u7814\u7a76\uff0c\u8be5\u7814\u7a76\u8bbe\u8ba1\u4e86\u4e00\u79cd\u591a\u529f\u80fd\u9776\u5411\u7ec6\u80de\u6838\u7684\u201c\u6838\u58f3\u7ed3\u6784\u201d\u7684\u4eff\u751f\u75c5\u6bd2\u7eb3\u7c73\u7c92\u5b50\uff08RRPHC\uff09\u6765\u5b9e\u73b0\u5bf9\u7ec6\u80de\u6838\u7684\u9776\u5411\u9012\u9001<sup>[1]<\/sup>\u3002RRPHC\u4eff\u751f\u75c5\u6bd2(RRPHC\/Cas9-hMTH1)\u7531\u4e00\u79cd\u6c1f\u5316\u805a\u5408\u7269(PF<sub>33<\/sub>)\u6765\u5b9e\u73b0\u5bf9CRISPR-Cas9\u7cfb\u7edf\u7684\u7ed3\u5408(PF<sub>33<\/sub>\/Cas9-hMTH1\u7eb3\u7c73\u7c92\u5b50)\uff0c\u548c\u4e00\u4e2a\u591a\u529f\u80fd\u7eb3\u7c73\u6750\u6599\u4fee\u9970\u7684\u5916\u58f3(RGD-R8-PEG-HA, RRPH)\u5171\u540c\u7ec4\u6210\u3002\u8fd9\u6837\u7684\u591a\u5c42\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u5916\u8868\u4fee\u9970\u4e86PEG\u4fa7\u94fe\u548c\u9776\u5411\u80bdRGD\u4fee\u9970\u7684\u900f\u660e\u8d28\u9178HA\uff0c\u8d4b\u4e88\u4e86\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u7684\u6df1\u5ea6\u7a7f\u900f\u80fd\u529b\u3002HA\u53ef\u4ee5\u591a\u79cd\u80bf\u7624\u8868\u9762\u8fc7\u8868\u8fbe\u7684CD44\u53d7\u4f53\u8d77\u5230\u9776\u5411\u4f5c\u7528\uff0c\u540c\u65f6\u5e26\u8d1f\u7535\u8377\u7684HA\u53ef\u4ee5\u5728\u7ec6\u80de\u5916\u57fa\u8d28\u4e2d\u7684\u900f\u660e\u8d28\u9178\u9176HAase\u7684\u4f5c\u7528\u4e0b\u53d1\u751f\u964d\u89e3\uff0c\u66b4\u9732\u51fa\u6b63\u7535\u8377\u7684PF<sub>33<\/sub>\/Cas9-hMTH1\u5185\u6838\u7ed3\u6784\uff0c\u4fc3\u8fdb\u7ec6\u80de\u5185\u541e\u548c\u907f\u514d\u7cfb\u7edf\u7ed9\u836f\u7684\u5feb\u901f\u6e05\u9664\u4f5c\u7528\u3002\u6b64\u5916\uff0cRGD\u591a\u80bd\u5bf9\u80bf\u7624\u8840\u7ba1\u5185\u76ae\u7ec6\u80de\u4e0a\u7684\u03b1v\u03b23\u6574\u5408\u7d20\u6709\u9776\u5411\u4f5c\u7528\uff0c\u8fdb\u4e00\u6b65\u5b9e\u73b0\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u7684\u6df1\u5c42\u7a7f\u900f\u6027\u3002\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u8fd9\u6837\u7684\u57fa\u4e8e\u591a\u5c42\u529f\u80fd\u6027\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u7684\u4eff\u751f\u75c5\u6bd2\u901a\u8fc7\u53cc\u91cd\u53d7\u4f53\u4ecb\u5bfc\u7684\u5185\u541e\u4f5c\u7528\u6709\u6548\u7684\u5c06CRISPR\/Cas9\u590d\u5408\u7269\u9776\u5411\u9012\u9001\u5230\u5375\u5de2\u764c\uff0c\u5e76\u4e14\u526f\u4f5c\u7528\u8f83\u5c0f\uff0c\u89e3\u51b3\u4e86\u4f8b\u5982\u817a\u75c5\u6bd2\u4e00\u7c7b\u4f20\u7edf\u7684\u75c5\u6bd2\u8f7d\u4f53\u5b89\u5168\u6027\u4ee5\u53ca\u6548\u7387\u4f4e\u7684\u9012\u9001\u95ee\u9898\u3002<\/p>\n<p><strong><b>\u56fe\u4e00\uff1a\u591a\u5c42\u529f\u80fd\u578b\u201c\u4eff\u751f\u75c5\u6bd2\u201d\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u793a\u610f\u56fe<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189623 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72471-12.png\" alt=\"\" width=\"680\" height=\"230\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72471-12.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72471-12-300x101.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p>\u7531\u4e8eCRISPR\/Cas9\u590d\u5408\u7269\u5728\u7ec6\u80de\u6838\u5185\u7ecf\u8fc7\u6eb6\u9176\u4f53\u5185\u8fc7\u9178\u6027\u7684\u73af\u5883\uff0c\u4f1a\u5f88\u5feb\u88ab\u964d\u89e3\u800c\u5931\u53bb\u4f5c\u7528\uff0c\u5927\u5927\u964d\u4f4e\u4e86\u57fa\u56e0\u7f16\u8f91\u7684\u6548\u7387\u3002\u56e0\u6b64\uff0c\u9700\u8981\u5bfb\u6c42\u4e00\u79cd\u5feb\u901f\u6eb6\u9176\u4f53\u9003\u9038\uff0c\u4fdd\u62a4\u86cb\u767d\u590d\u5408\u7269\u7684\u65b9\u5f0f\u6765\u63d0\u9ad8\u9012\u9001\u6548\u7387\u3002\u4f20\u7edf\u7684\u57fa\u56e0\u8f7d\u4f53\u4f1a\u9009\u62e9\u6b63\u7535\u6027\u8f83\u5f3a\u4e14\u80fd\u5438\u8d28\u5b50H<sup>+<\/sup>\u7684\u805a\u5408\u7269\u5982\u805a\u4e59\u70ef\u4e9a\u80faPEI\uff0c\u7136\u540ePEI\u5728\u5e94\u7528\u4e2d\u7531\u4e8e\u5b58\u5728\u8f83\u5927\u7684\u6bd2\u6027\u800c\u53d7\u5230\u4e86\u4e00\u5b9a\u7684\u9650\u5236\u3002\u56e0\u6b64\uff0c\u4e00\u4e9b\u7814\u7a76\u9010\u6e10\u805a\u7126\u4e8e\u5b89\u5168\u4e14\u6709\u6548\u7684\u9633\u79bb\u5b50\u805a\u5408\u7269\u6765\u73b0\u5b9e\u9ad8\u6548\u7684\u6eb6\u9176\u4f53\u9003\u9038\u30022017\u5e74\uff0c\u53d1\u8868\u5728\u56fd\u9645\u671f\u520a<strong>Nature\u00a0Biomedical\u00a0Engineering<\/strong>\u00a0\u4e0a\u9762\u7684\u4e00\u9879\u7814\u7a76\uff0c\u8be5\u7814\u7a76\u91c7\u7528\u4e86\u751f\u7269\u76f8\u5bb9\u6027\u597d\u7684\u91d1\u7eb3\u7c73\u7c92\u5b50AuNP\u4f5c\u4e3a\u5185\u6838\uff0c\u53ef\u4ee5\u7528\u6765\u5c01\u88c5CRISPR\/Cas9\u590d\u5408\u7269<sup>[2]<\/sup>\u3002\u5916\u58f3\u5305\u88f9\u4e00\u5c42\u751f\u7269\u5b89\u5168\u6027\u4ee5\u53ca\u7ec6\u80de\u76f8\u5bb9\u6027\u826f\u597d\u7684\u9633\u79bb\u5b50\u805a\u5929\u51ac\u9170\u80fa\u884d\u751f\u7269PASp(DET) \u4f5c\u4e3a\u5185\u6db5\u4f53\u9003\u9038\u7684\u4fdd\u62a4\u58f3\u6765\u5b9e\u73b0\u5bf9\u540c\u6e90\u4ecb\u5bfc\u7684\u53cc\u94feDNA\u4fee\u590d\uff0c\u4ece\u800c\u5bf9\u675c\u6c0f\u808c\u8425\u517b\u4e0d\u826f\u75c7\u7684\u5c0f\u9f20\u8fbe\u5230\u4e00\u4e2a\u826f\u597d\u7684\u6cbb\u7597\u3002<\/p>\n<p><strong><b>\u56fe\u4e8c\uff1a<\/b><\/strong><strong><b>CRISPR\u2013Gold<\/b><\/strong><strong><b>\u7eb3\u7c73\u590d\u5408\u7269\u5f62\u6210\u53ca\u7ec6\u80de\u5185\u5316\u539f\u7406<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189625 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72472-12.png\" alt=\"\" width=\"680\" height=\"304\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72472-12.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72472-12-300x134.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p>\u9664\u6b64\u4e4b\u5916\uff0c2017\u5e74\u53d1\u8868\u5728<strong>ACS Nano<\/strong>\u4e0a\u9762\u7684\u6587\u7ae0\u4e5f\u62a5\u9053\u4e86\u4e00\u79cd\u57fa\u4e8e\u91d1\u7eb3\u7c73\u7c92\u5b50\u5185\u6838\u7684\u975e\u75c5\u6bd2\u7eb3\u7c73\u8f7d\u4f53<sup>[3]<\/sup>\u3002\u8be5\u7eb3\u7c73\u8fd0\u8f7d\u7cfb\u7edf\u4ee5\u7cbe\u6c28\u9178\u4fee\u9970\u7684\u91d1\u7eb3\u7c73\u7c92\u5b50\u4e3a\u5185\u6838\uff0c\u901a\u8fc7Au-S\u952e\u4f5c\u7528\u5c06Cas9\u86cb\u767d\u548csgRNA\u5171\u540c\u81ea\u7ec4\u88c5\u6210\u7eb3\u7c73\u590d\u5408\u7269\uff0c\u901a\u8fc7\u819c\u878d\u5408\u7684\u65b9\u5f0f\u88ab\u7ec6\u80de\u6444\u5165\u3002\u7531\u4e8e\u7cbe\u6c28\u9178\u5e26\u6709\u5927\u91cf\u6c28\u57fa\uff0c\u53ef\u4ee5\u5f88\u597d\u7684\u5438\u6536\u8d28\u5b50\u4fdd\u62a4\u86cb\u767d\u590d\u5408\u7269\u5728\u6eb6\u9176\u4f53\u5185\u9178\u6027\u73af\u5883\u4e0d\u53d7\u7834\u574f\uff0c\u4ece\u800c\u5b9e\u73b0\u6709\u6548\u7684\u5c06\u5176\u5730\u9001\u5230\u7ec6\u80de\u6838\u53d1\u6325\u4f5c\u7528\u3002\u8be5\u6587\u7ae0\u8868\u660e\uff0c\u57fa\u4e8e\u819c\u878d\u5408\u88ab\u7ec6\u80de\u5185\u5316\u7684\u7eb3\u7c73\u7cfb\u7edf\u53ef\u4ee5\u8fbe\u5230\u5927\u7ea690%\u7684\u9012\u9001\u6548\u7387\uff0c\u540c\u65f6\u5bf9\u4e8e\u4e0d\u540c\u7684\u7ec6\u80de\u7cfb\u53ef\u4ee5\u8fbe\u5230\u7ea630%\u7684\u57fa\u56e0\u7f16\u8f91\u6548\u7387\u3002<\/p>\n<p><strong><b>\u56fe\u4e09\uff1a<\/b><\/strong><strong><b>CRISPR\u2013A<\/b><\/strong><strong><b>rg<\/b><\/strong><strong><b>NP<\/b><\/strong><strong><b>s\u4f5c\u7528\u673a\u7406\u793a\u610f\u56fe<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189626 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72473-11.png\" alt=\"\" width=\"680\" height=\"448\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72473-11.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72473-11-300x198.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p>\u5c3d\u7ba1\u9633\u79bb\u5b50\u805a\u5408\u7269\u53ef\u4ee5\u4fdd\u62a4\u86cb\u767d\u590d\u5408\u7269\u901a\u8fc7\u6eb6\u9176\u4f53\u9003\u9038\uff0c\u4f46\u8f83\u5f3a\u7684\u6b63\u7535\u6027\u4f7f\u5f97\u5176\u5728\u7cfb\u7edf\u7ed9\u836f\u4e4b\u540e\u5f88\u5bb9\u6613\u88ab\u6e05\u9664\uff0c\u4ecd\u7136\u5b58\u5728\u4e00\u4e9b\u5c40\u9650\u6027\u4f7f\u5176\u4e0d\u80fd\u5f88\u597d\u7684\u8fdb\u5165\u4e34\u5e8a\u5e94\u7528\u30022016\u5e74\u548c2019\u5e74\u5206\u522b\u53d1\u8868\u5728<strong>PNAS<\/strong>\u548c<strong>Advanced\u00a0Materials<\/strong>\u4e0a\u9762\u7684\u4e24\u9879\u7814\u7a76\u62a5\u9053\u4e86\u8fc4\u4eca\u4e3a\u6b62\u7eb3\u7c73\u6750\u6599\u9012\u9001CRISPR\/Cas9\u590d\u5408\u7269\u6700\u4e3a\u6709\u6548\u7684\u624b\u6bb5\u4e4b\u4e00\u3002\u8fd9\u4e24\u9879\u7814\u7a76\u90fd\u62a5\u9053\u4e86\u4e00\u79cd\u751f\u7269\u53ef\u964d\u89e3\u7684\u5408\u6210\u8102\u8d28\u7eb3\u7c73\u7c92\u5b50\uff0c\u8102\u8d28\u7eb3\u7c73\u9897\u7c92\u901a\u8fc7\u9759\u7535\u4f5c\u7528\u5438\u9644\u4e86Cas9\u7684\u4fe1\u4f7fRNA\uff08mRNA\uff09<sup>[4,5]<\/sup>\u3002\u5f53\u8fd9\u4e9b\u5305\u542bsgRNA\u7684\u7eb3\u7c73\u9897\u7c92\u7684\u5185\u542b\u7269\u91ca\u653e\u5230\u7ec6\u80de\u4e2d\uff0c\u7ec6\u80de\u4e2d\u7684\u86cb\u767d\u5236\u9020\u5de5\u5382\u63a5\u7ba1\u8fd9\u79cdmRNA\u6a21\u677f\uff0c\u5e76\u5229\u7528\u8fd9\u79cd\u6a21\u677f\u8868\u8fbeCas9\u86cb\u767d\uff0c\u4ece\u800c\u5b9e\u73b0\u8fd9\u79cd\u57fa\u56e0\u7f16\u8f91\u5de5\u5177\u7684\u4f5c\u7528\u3002\u8fd9\u4e9b\u7eb3\u7c73\u9897\u7c92\u7684\u5171\u540c\u7279\u5f81\u5728\u4e8e\u5b83\u4eec\u662f\u7531\u8102\u80aa\u94fe\u4e2d\u542b\u6709\u4e8c\u786b\u952e\u7684\u5408\u6210\u8102\u8d28\u81ea\u7ec4\u88c5\u5f62\u6210\u7684\u3002\u5f53\u8fd9\u4e9b\u7eb3\u7c73\u7c92\u5b50\u8fdb\u5165\u7ec6\u80de\u4e2d\u65f6\uff0c\u7ec6\u80de\u4e2d\u7684\u73af\u5883\u7834\u574f\u4e86\u4e8c\u786b\u952e\u800c\u5c06\u5b83\u4eec\u62c6\u89e3\u5f00\uff0c\u4ece\u800c\u5bfc\u81f4\u5b83\u4eec\u4e2d\u7684\u5185\u542b\u7269\u5feb\u901f\u9ad8\u6548\u5730\u91ca\u653e\u5230\u7ec6\u80de\u4e2d\uff0c\u540c\u65f6\u7814\u7a76\u62a5\u9053\u8fd9\u79cd\u57fa\u4e8e\u8fd8\u539f\u54cd\u5e94\u7684\u7eb3\u7c73\u8102\u8d28\u4f53\u5bf9\u57fa\u56e0\u7f16\u8f91\u6548\u7387\u53ef\u8fbe90%\u3002<\/p>\n<p><strong><b>\u56fe\u56db\uff1a\u57fa\u4e8e\u8fd8\u539f\u54cd\u5e94\u7eb3\u7c73\u8102\u8d28\u4f53<\/b><\/strong><strong><b>CRISPR\/C<\/b><\/strong><strong><b>as9\u590d\u5408\u7269\u7684\u4f5c\u7528\u673a\u7406\u793a\u610f\u56fe<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189627 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72474-10.png\" alt=\"\" width=\"680\" height=\"364\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72474-10.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72474-10-300x161.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p>\u7531\u4e8eCRISPR\/Cas9\u662f\u5728\u57fa\u56e0\u5c42\u9762\u5bf9\u7ec6\u80deDNA\u8fdb\u884c\u5220\u9664\u6216\u8005\u63d2\u5165\uff0c\u6240\u4ee5\u5176\u4f5c\u7528\u5230\u7684\u9776\u5668\u5b98\u4e0e\u5176\u4ed6\u6b63\u5e38\u7ec4\u7ec7\u5668\u5b98\u5c06\u4f1a\u5b58\u5728\u6f5c\u5728\u7684\u81f4\u764c\u98ce\u9669\u3002\u56e0\u6b64\uff0c\u9009\u62e9\u6027\u7684\u6fc0\u6d3b\u7279\u5b9a\u7ec4\u7ec7\u6216\u5668\u5b98\u4e2d\u7684CRISPR\/Cas9\u590d\u5408\u7269\u7684\u8868\u8fbe\uff0c\u4ee5\u8fbe\u5230\u6700\u5927\u5316\u7684\u6cbb\u7597\u6548\u679c\uff0c\u5b9e\u73b0\u6700\u5c0f\u5316\u7684\u7cfb\u7edf\u6bd2\u6027\u30022019\u5e74\u53d1\u8868\u5728<strong>Nature\u00a0Biomedical\u00a0Engineering<\/strong>\u4e0a\u9762\u7684\u4e00\u9879\u7814\u7a76\u62a5\u9053\u4e86\u4e00\u79cd\u65b0\u578b\u7684\u78c1\u573a\u63a7\u5236\u7684\u57fa\u56e0\u8f7d\u4f53\u7cfb\u7edf<sup>[6]<\/sup>\u3002\u8fd9\u6837\u7684\u4e00\u79cdCRISPR\u9012\u9001\u7cfb\u7edf\u4e3b\u8981\u7531\u4e00\u79cd\u5927\u5c3a\u5bf8\u7684\u6746\u72b6\u75c5\u6bd2\u5b9e\u73b0\u5bf9CRISPR\u590d\u5408\u7269\u7684\u5305\u8f7d\uff0c\u540c\u65f6\u5728\u75c5\u6bd2\u8868\u9762\u9759\u7535\u590d\u5408\u4e86\u7a7f\u6a21\u80bdTAT\u4fee\u9970\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u3002\u8be5\u7cfb\u7edf\u53ef\u4ee5\u5728\u78c1\u573a\u7684\u4f5c\u7528\u4e0b\u6709\u6548\u7684\u5bcc\u96c6\u5728\u809d\u810f\u7ec4\u7ec7\u4e2d\uff0c\u540c\u65f6\u5728\u78c1\u573a\u7684\u63a7\u5236\u4e0b\u5b9e\u73b0\u5bf9\u4f53\u5185\u57fa\u56e0\u7f16\u8f91\u7684\u7a7a\u95f4\u63a7\u5236\u3002<\/p>\n<p><strong><b>\u56fe\u4e94\uff1a\u57fa\u4e8e\u78c1\u573a\u63a7\u5236\u7684<\/b><\/strong><strong><b>CRISPR\/C<\/b><\/strong><strong><b>as9\u590d\u5408\u7269\u793a\u610f\u56fe<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189628 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72475-9.png\" alt=\"\" width=\"680\" height=\"198\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72475-9.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72475-9-300x87.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p>\u9664\u4e86\u78c1\u573a\u63a7\u5236\uff0c\u8fd8\u6709\u4e00\u4e9b\u7814\u7a76\u805a\u7126\u4e8e\u5229\u7528\u5149\u63a7\u5236CRISPR\/Cas9\u590d\u5408\u7269\u5728\u7279\u5b9a\u7ec4\u7ec7\u7684\u7a7a\u95f4\u63a7\u5236\u30022019\u5e74\u53d1\u8868\u5728<strong>Science\u00a0Advances<\/strong>\u4e0a\u9762\u7684\u4e00\u9879\u62a5\u9053\uff0c\u5b9e\u73b0\u4e86\u7528\u8fd1\u7ea2\u5916\u5149\u5bf9CRISPR\/Cas9\u590d\u5408\u7269\u7684\u5b9a\u70b9\u63a7\u5236\u91ca\u653e<sup>[7]<\/sup>\u3002\u8fd9\u6837\u7684\u5149\u63a7\u5236\u8f7d\u4f53\u7cfb\u7edf\u7531\u9567\u7cfb\u5143\u7d20\u63ba\u6742\u7684\u4e0a\u8f6c\u6362\u7eb3\u7c73\u7c92\u5b50\uff08upconversion nanoparticle, UCNP\uff09\u7ec4\u6210\u3002\u5176\u4e2d\uff0cUCNP\u4fee\u9970\u4e86\u7d2b\u5916\u654f\u611f\u65ad\u952e\u7684\u5316\u5b66\u57fa\u56e2ONA\uff0c\u540c\u65f6\u590d\u5408CRISPR\/Cas9\u86cb\u767d\u5e76\u5728\u5916\u5c42\u5305\u88f9\u4e86PEI\u5b9e\u73b0\u6709\u6548\u7684\u6eb6\u9176\u4f53\u9003\u9038\u3002\u5f53\u8fd1\u7ea2\u5916\u5149\u7167\u5c04\u65f6\uff0c\u8fd9\u79cd\u5149\u88ab\u5438\u6536\u5e76\u8f6c\u6362\u6210\u7d2b\u5916\u5149\u8fdb\u800c\u5f15\u8d77\u5316\u5b66\u952e\u65ad\u88c2\uff0c\u4ece\u800c\u5b9e\u73b0\u5bf9CRISPR\/Cas9\u590d\u5408\u7269\u7684\u5207\u5272\u91ca\u653e\uff0c\u5230\u8fbe\u8fd1\u7ea2\u5916\u5149\u6fc0\u6d3b\u7684\u7a7a\u95f4\u63a7\u5236\u3002\u4e0e\u6b64\u540c\u65f6\uff0c\u5728<strong>Advanced\u00a0Materials<\/strong>\u4e0a\u9762\u4e5f\u53d1\u8868\u4e86\u5149\u63a7\u5236CRISPR\/Cas9\u8868\u8fbe\u7684\u76f8\u5173\u7814\u7a76\uff0c\u8be5\u7814\u7a76\u62a5\u9053\u4e86\u4e00\u79cd\u5237\u72b6\u7ed3\u6784\u7684\u542b\u6c1f\u5316\u805a\u4e59\u70ef\u4e9a\u80fa\uff08PF\uff09\u7684\u534a\u5bfc\u4f53\u805a\u5408\u7269\uff0c\u5176\u4e2dPF\u53ef\u4ee5\u4e0eCRISPR\/Cas9\u7ed3\u5408\uff0c\u540c\u65f6\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u5185\u6838\u5305\u8f7d\u4e86\u4e00\u79cd\u53ef\u4ee5\u4f7f\u6838\u6269\u5f20\u7684\u7cd6\u76ae\u8d28\u6fc0\u7d20<sup>[8]<\/sup>\u3002\u805a\u5408\u7269\u5916\u58f3\u4fee\u9970\u4e86\u805a\u4e59\u4e8c\u9187PEG\uff0c\u901a\u8fc7\u8d85\u5206\u5b50\u548c\u9759\u7535\u4f5c\u7528\u5f62\u6210\u534a\u5bfc\u4f53\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u3002\u5f53\u8be5\u805a\u5408\u7269\u7eb3\u7c73\u7c92\u5b50\u5728\u7279\u5b9a\u7ec4\u7ec7\u5bcc\u96c6\u4e4b\u540e\uff0c\u7ed9\u4e0e\u8fd1\u7ea2\u5916\u5149\u7167\uff0c\u534a\u5bfc\u4f53\u805a\u5408\u7269\u8fdb\u884c\u5149\u70ed\u8f6c\u6362\u4e4b\u540e\u5feb\u901f\u91ca\u653e\u51faCRISPR\/Cas9\u590d\u5408\u7269\u3002\u4ee5\u4e0a\u4e24\u9879\u7814\u7a76\u90fd\u5229\u7528\u5916\u754c\u5149\u523a\u6fc0\uff0c\u5b9e\u73b0\u4f53\u5185\u57fa\u56e0\u7f16\u8f91\u7684\u7a7a\u95f4\u63a7\u5236\uff0c\u901a\u8fc7\u65e0\u521b\u9065\u63a7\uff0c\u5de7\u5999\u5730\u5229\u7528\u667a\u80fd\u578b\u7eb3\u7c73\u6750\u6599\uff0c\u5c06\u63d0\u9ad8\u4f53\u5185CRISPR\/Cas9\u7f16\u8f91\u7684\u51c6\u786e\u6027\u548c\u5b89\u5168\u6027\u3002<\/p>\n<p><strong><b>\u56fe\u516d\uff1a\u57fa\u4e8e\u5149\u63a7\u5236<\/b><\/strong><strong><b>CRISPR\/C<\/b><\/strong><strong><b>as9\u590d\u5408\u7269\u7684\u793a\u610f\u56fe<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-189629 aligncenter\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72476-9.png\" alt=\"\" width=\"680\" height=\"641\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72476-9.png 680w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2019\/11\/\u56fe\u72476-9-300x283.png 300w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<h3><strong><b>\u603b\u7ed3\u4e0e\u5c55\u671b<\/b><\/strong><\/h3>\n<p>\u8fd1\u5e74\u6765\u5927\u91cf\u7684\u7814\u7a76\u901a\u8fc7\u667a\u80fd\u578b\u7684\u7eb3\u7c73\u7cfb\u7edf\u53bb\u5bf9CRISPR\/Cas9\u7684\u53ef\u63a7\u7f16\u8f91\u4ee5\u53ca\u9012\u9001\u6548\u7387\u4e0d\u65ad\u5730\u8fdb\u884c\u5b8c\u5584\u548c\u4f18\u5316\uff0c\u4e0d\u8bba\u662f\u901a\u8fc7\u8bbe\u8ba1\u7cbe\u5de7\u800c\u590d\u6742\u7684\u591a\u91cd\u529f\u80fd\u7684\u7eb3\u7c73\u6750\u6599\u8fd8\u662f\u5229\u7528\u5916\u754c\u523a\u6fc0\uff0c\u5982\u5149\u3001\u58f0\u3001\u78c1\u3001\u70ed\u7b49\u5bf9CRISPR\/Cas\u5b9e\u73b0\u7a7a\u95f4\u7684\u8fdc\u7a0b\u63a7\u5236\uff0c\u591a\u529f\u80fd\u7684\u667a\u80fd\u7eb3\u7c73\u6750\u6599\u5177\u6709\u53ef\u8c03\u8282\u6027\uff0c\u7075\u6d3b\u6027\uff0c\u548c\u53ef\u63a7\u6027\uff0c\u76f8\u8f83\u4e8e\u4f20\u7edf\u7684\u57fa\u56e0\u8f7d\u4f53\u5bf9CRISPR\/Cas9\u7f16\u8f91\u7cfb\u7edf\u90fd\u5b9e\u73b0\u4e86\u6709\u6548\u7684\u9012\u9001\u5e76\u4e14\u5927\u5927\u7684\u63d0\u9ad8\u4e86\u7f16\u8f91\u6548\u7387\u3002\u57fa\u4e8e\u4ee5\u4e0a\u7684\u4e00\u4e9b\u8fdb\u5c55\u68b3\u7406\uff0c\u8ba9\u6211\u4eec\u62ed\u76ee\u4ee5\u5f85CRISPR\/Cas9\u4e0e\u7eb3\u7c73\u6750\u6599\u7684\u7cbe\u5999\u7ed3\u5408\uff0c\u4f7f\u5176\u80fd\u591f\u5c3d\u65e9\u7684\u8f6c\u5316\u5230\u4e34\u5e8a\u5e94\u7528\u4e4b\u4e0a\uff01<\/p>\n<h3><strong><b>\u53c2\u8003\u6587\u732e<\/b><\/strong><\/h3>\n<p>[1] Artificial Virus Delivers CRISPR-Cas9 System for Genome Editing of Cells in Mice.\u00a0ACS Nano, 2017, 11, 95\u2212111, DOI: 10.1021\/acsnano.6b04261<\/p>\n<p>[2] Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo inducehomology-directed DNA repair. NatureBiomedical\u00a0Engineering, DOI: 10.1038\/s41551-017-0137-2<\/p>\n<p>[3] Direct Cytosolic Delivery of CRISPR\/Cas9-Ribonucleoprotein for Efficient Gene Editing. ACS Nano, 2017, 11, 2452\u22122458, DOI: 10.1021\/acsnano.6b07600<\/p>\n<p>[4] Efficient delivery of genome-editing proteins using bio-reducible lipid nanoparticles. PNAS, 2016, 2868\u20132873, doi\/10.1073\/pnas.1520244113<\/p>\n<p>[5] Fast and Effcient CRISPR\/Cas9 Genome Editing In Vivo Enabled by Bio-reducible Lipid and Messenger RNA Nanoparticles. Advanced Materials, 2019, 1902575, DOI: 10.1002\/adma.201902575<\/p>\n<p>[6] Spatial control of in vivo CRISPR\u2013Cas9 genome editing via nanomagnets. NatureBiomedical\u00a0Engineering, doi.org\/10.1038\/s41551-018-0318-7<\/p>\n<p>[7] Near-infrared upconversion\u2013activated CRISPR-Cas9 system: A remote-controlled gene editing platform. 2019; 5 eaav7199, DOI: 10.1126\/sciadv.aav7199<\/p>\n<p>[8] A Rationally Designed Semiconducting Polymer Brush for NIR-II Imaging-Guided Light-Triggered Remote Control of CRISPR\/Cas9 Genome Editing. Advanced Materials, 2019, 1901187, DOI: 10.1002\/adma.201901187<\/p>\n<p>\u672c\u6587\u7531<strong>\u867e\u7c73<\/strong>\u4f9b\u7a3f\u3002<\/p>\n<p>\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1: tougao@cailiaoren.com.<\/p>\n<p>\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenVIP.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd1\u5e74\u6765\uff0c\u57fa\u4e8eCRISPR-Cas9\u7684\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5728\u6574\u4e2a\u79d1\u5b66\u9886\u57df\u90fd\u5f15\u8d77\u4e86\u4f17\u591a\u7814\u7a76\u5b66\u8005\u7684\u5174\u8da3\u3002\u4f5c\u4e3a\u4e00\u79cd\u57fa\u56e0\u526a\u8f91\u7cfb\u7edf\uff0c\u5b83\u53ef\u4ee5\u5bf9\u67d0\u4e00\u6838\u82f7\u9178\u5e8f\u5217\u4e2d\u7684\u7279\u5b9a\u57fa\u56e0\u4f4d\u70b9\u8fdb\u884c\u4eba\u4e3a\u6539&#8230;<\/p>\n","protected":false},"author":10213,"featured_media":188163,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1187],"tags":[],"class_list":["post-189612","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-zhuanlan"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/189612"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/10213"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=189612"}],"version-history":[{"count":2,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/189612\/revisions"}],"predecessor-version":[{"id":189836,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/189612\/revisions\/189836"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/media\/188163"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=189612"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=189612"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=189612"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}