{"id":198607,"date":"2020-04-03T06:25:04","date_gmt":"2020-04-02T22:25:04","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=198607"},"modified":"2020-04-02T21:27:26","modified_gmt":"2020-04-02T13:27:26","slug":"%e5%b7%b2%e6%a0%a1%e7%a8%bf%e4%bb%b6-%e4%b8%ad%e7%a7%91%e9%99%a2%e6%9c%b1%e6%98%8e%e6%98%ad%e5%9b%a2%e9%98%9fnat-nanotech-%e6%8a%a5%e9%81%93%ef%bc%9a%e5%8f%8c%e9%9d%b6%e5%90%91%e9%93%81%e8%9b%8b","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=198607","title":{"rendered":"\u4e2d\u79d1\u9662\u6731\u660e\u662d\u56e2\u961fNat. Nanotech.\u62a5\u9053\uff1a\u53cc\u9776\u5411\u94c1\u86cb\u767d\u7eb3\u7c73\u75ab\u82d7\u5f15\u53d1\u9488\u5bf9\u6162\u6027\u4e59\u578b\u809d\u708e\u7684\u6cbb\u7597\u6027\u6297\u4f53\u53cd\u5e94"},"content":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011<\/strong><\/p>\n

\u4e59\u578b\u809d\u708e\uff08CHB\uff09\u662f\u7531\u4e59\u578b\u809d\u708e\u75c5\u6bd2\uff08HBV\uff09\u611f\u67d3\u5f15\u8d77\u7684\u5168\u7403\u6027\u5065\u5eb7\u95ee\u9898\uff0cHBV\u4f1a\u653b\u51fb\u809d\u810f\uff0c\u4ece\u800c\u5bfc\u81f4\u6025\u6027\u548c\u6162\u6027\u75be\u75c5\u3002\u636e\u4f30\u8ba1\uff0c\u76ee\u524d\u5168\u7403\u67092.57\u4ebf\u4eba\u662fHBV\u6162\u6027\u643a\u5e26\u8005\uff0c\u5e76\u6709\u53d1\u5c55\u4e3a\u809d\u75c5\u3001\u809d\u786c\u5316\u548c\u809d\u7ec6\u80de\u764c\u7684\u9ad8\u98ce\u9669\u3002\u867d\u7136\u5e38\u89c4\u7684\u4e59\u578b\u809d\u708e\u8868\u9762\u6297\u539f\uff08HBsAg\uff09\u75ab\u82d7\u63a5\u79cd\u53ef\u5728\u5927\u591a\u6570\u5065\u5eb7\u7684\u63a5\u79cd\u4eba\u7fa4\u4e2d\u8bf1\u5bfc\u4fdd\u62a4\u6027\u6297\u4f53\uff0c\u5e76\u6709\u6548\u964d\u4f4e\u65b0\u7684HBV\u611f\u67d3\u7684\u53d1\u751f\u7387\uff0c\u4f46\u662fHBsAg\u5728\u4e34\u5e8a\u52a8\u7269\u6a21\u578b\u6216CHB\u611f\u67d3\u60a3\u8005\u4e2d\u5747\u65e0\u6cd5\u8bf1\u5bfc\u6709\u6548\u7684\u6297\u4f53\u53cd\u5e94\u3002\u56e0\u6b64\uff0c\u975e\u5e38\u9700\u8981\u4e00\u79cd\u6d88\u9664\u548c\u6839\u9664CHB\u7684\u6709\u6548\u6cbb\u7597\u7b56\u7565\u3002<\/p>\n

\u5bf9\u6bd4HBsAg\uff0cHBV\u5927\u8868\u9762\u6297\u539f\u7684preS1\u7ed3\u6784\u533a\u57df\u88ab\u8ba4\u4e3a\u662f\u6cbb\u7597\u6027\u75ab\u82d7\u5f00\u53d1\u7684\u7406\u60f3\u514d\u75ab\u539f\u5019\u9009\u7269\u3002\u76ee\u524d\u5df2\u8bc1\u660e\u9488\u5bf9preS1\u7684\u5355\u514b\u9686\u6297\u4f53\uff08MA18\/7\u548cKR127\uff09\u5728\u4f53\u5185\u5916\u5747\u80fd\u6709\u6548\u6291\u5236HBV\u611f\u67d3\u3002\u540c\u65f6\uff0c\u6297-preS1\u8fd8\u53ef\u4ee5\u901a\u8fc7\u6297\u4f53\u4f9d\u8d56\u6027\u7ec6\u80de\u4ecb\u5bfc\u7684\u7ec6\u80de\u6bd2\u6027\u548c\u541e\u566c\u4f5c\u7528\u6d88\u9664HBV+<\/sup>\u7ec6\u80de\u3002\u6b64\u5916\uff0c\u5728CHB\u60a3\u8005\u4e2d\uff0c\u5bf9preS1\u7684\u514d\u75ab\u8010\u53d7\u6027\u4f3c\u4e4e\u6bd4\u5bf9HBsAg\u7684\u514d\u75ab\u8010\u53d7\u6027\u4f4e\u5f97\u591a\u3002\u7814\u7a76\u663e\u793a\uff0cpreS1\u6297\u539f\u75ab\u82d7\u63a5\u79cd\u800c\u975eHBsAg\u75ab\u82d7\u5728HBV\u643a\u5e26\u8005\u5c0f\u9f20\u4e2d\u5f15\u8d77\u4e2d\u548c\u6027\u6cbb\u7597\u6297\u4f53\u5e94\u7b54\u3002\u5728\u81ea\u7136\u611f\u67d3\u548c\u4e3b\u52a8\u63a5\u79cd\u4e2d\u5747\u53d1\u73b0\uff0cpreS1\u4f3c\u4e4e\u4e0d\u662f\u6709\u6548\u7684\u6297\u4f53\u8bf1\u5bfc\u5242\u3002\u56e0\u6b64\uff0c\u5982\u4f55\u63d0\u9ad8preS1\u7684\u514d\u75ab\u539f\u6027\u4ee5\u589e\u5f3a\u6cbb\u7597\u6027\u6297\u4f53\u53cd\u5e94\u662f\u5f53\u524d\u5f00\u53d1\u57fa\u4e8epreS1\u7684\u6cbb\u7597\u6027\u75ab\u82d7\u7684\u5173\u952e\u95ee\u9898\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u57fa\u4e8e\u6b64\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u751f\u7269\u7269\u7406\u6240\u7684\u6731\u660e\u662d\u7814\u7a76\u5458\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u56e2\u961f\u62a5\u9053\u4e86\u4e00\u79cd\u94c1\u86cb\u767d\u7eb3\u7c73\u7c92\u5b50\u75ab\u82d7\uff0c\u53ef\u4ee5\u5c06preS1\u9012\u9001\u81f3\u7279\u5b9a\u7684\u9aa8\u9ad3\u7ec6\u80de\uff0c\u5305\u62ecSIGNR1+<\/sup>\u6811\u7a81\u72b6\u7ec6\u80de\uff08\u6fc0\u6d3bT\u6ee4\u6ce1\u7684\u8f85\u52a9\u7ec6\u80de\uff09\u548c\u4e0e\u6dcb\u5df4\u7aa6\u76f8\u5173\u7684SIGNR1+<\/sup>\u5de8\u566c\u7ec6\u80de\uff08\u53ef\u4ee5\u6fc0\u6d3bB\u7ec6\u80de\uff09\u3002\u8fd9\u79cd\u7eb3\u7c73\u9897\u7c92\u75ab\u82d7\u53ef\u8bf1\u5bfc\u9ad8\u6c34\u5e73\u4e14\u6301\u4e45\u7684\u6297-preS1\u53cd\u5e94\uff0c\u4ece\u800c\u5728\u6162\u6027HBV\u5c0f\u9f20\u6a21\u578b\u4e2d\u5b9e\u73b0\u6709\u6548\u7684\u6e05\u9664\u75c5\u6bd2\u548c\u90e8\u5206\u8840\u6e05\u5b66\u8f6c\u5316\uff0c\u4e3a\u529f\u80fd\u6027\u6cbb\u6108\u6162\u6027\u4e59\u578b\u809d\u708e\u63d0\u4f9b\u4e86\u4e00\u79cd\u6709\u5e0c\u671b\u7684\u53ef\u8f6c\u5316\u75ab\u82d7\u63a5\u79cd\u7b56\u7565\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a\u201cDual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520aNature Nanotechnology<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/strong><\/p>\n

\u56fe\u4e00\u3001\u94c1\u86cb\u767dNP\u75ab\u82d7\u7684\u5206\u5b50\u8bbe\u8ba1\u548c\u8868\u5f81<\/strong>
\n \"\"\uff08a\uff09\u57fa\u4e8e\u94c1\u86cb\u767d\u7684\u75ab\u82d7\u9012\u9001\u8f7d\u4f53\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08b\uff09SpyTag-\u94c1\u86cb\u767dNPs\u7684\u5c3a\u5bf8\u6392\u963b\u8272\u8c31\uff1b<\/p>\n

\uff08c\uff09\u57284o<\/sup>C\u4e0b\uff0c\u4ee51\uff1a0.5\u30011\uff1a1\u548c1\uff1a2\u6469\u5c14\u6bd4\u5728SpyTag-\u94c1\u86cb\u767d\u548cSC-preS1\u7684\u5171\u4ef7\u8fde\u63a5\u5e76\u8fdb\u884c\u8fc7\u591c\u7684SDS-\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\u5206\u6790\uff1b<\/p>\n

\uff08d\uff09\u94c1\u86cb\u767dNP-preS1\u7684\u5c3a\u5bf8\u6392\u963b\u8272\u8c31\uff1b<\/p>\n

\uff08e\uff09SpyTag-\u94c1\u86cb\u767dNP\u548c\u94c1\u86cb\u767dNP-preS1\u7684TEM\u56fe\u548c2D\u91cd\u5efa\uff1b<\/p>\n

\uff08f\uff09\u5728\u7b2c21\u5929\u75ab\u82d7\u514d\u75ab\u65f6\uff0c\u5229\u7528\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a\uff08ELISA\uff09\u5206\u6790\u6297-preS1\u7684\u5e94\u7b54\uff1b<\/p>\n

\uff08g\uff09\u514d\u75ab\u540e\u6297-preS1\u6c34\u5e73\u7684\u52a8\u529b\u5b66\uff1b<\/p>\n

\uff08h\uff09\u6bd4\u8f83\u7ed3\u5408\u75ab\u82d7\u548c\u672a\u7ed3\u5408\u75ab\u82d7\u5bf9\u6297-preS1\u7684\u5e94\u7b54\uff1b<\/p>\n

\uff08i\uff09\u5229\u7528ELISA\u68c0\u6d4b\u6297preS1 IgG\u5bf9\u75ab\u82d7\u514d\u75ab\u7684\u4eb2\u548c\u529b\uff1b<\/p>\n

\uff08j\uff09\u7b2c21\u5929\uff0c\u94c1\u86cb\u767dNP-preS1\u75ab\u82d7\u6216SC-preS1\u514d\u75ab\u5c0f\u9f20\u7684\u8840\u6e05\u4e2dpreS1\u7279\u5f02\u6027IgG1\u548cIgG2c\u7684\u6ef4\u5ea6\u3002<\/p>\n

\u56fe\u4e8c\u3001\u94c1\u86cb\u767dNP-preS1\u75ab\u82d7\u5bf9HBV\u7684\u4fdd\u62a4\u6027\u514d\u75ab<\/strong>
\n \"\"\uff08a\uff09\u5728\u7b2c0\u5929\u548c\u7b2c14\u5929\u7528200 pmol\u94c1\u86cb\u767dNP-preS1\u75ab\u82d7\u6216\u7b49\u6469\u5c14SC-preS1\u53ef\u6eb6\u6027\u6297\u539f\u300130 \u03bcg CpG-1826\u76ae\u4e0b\u514d\u75ab\u5904\u7406\u7684WT C57BL\/6\u5c0f\u9f20\uff1b<\/p>\n

\uff08b\uff09\u5229\u7528ELISA\u68c0\u6d4bAAV-HBV1.3\u611f\u67d3\u540e\u7684preS1\u8840\u6e05\u6c34\u5e73\uff1b<\/p>\n

\uff08c\uff09\u5728\u7b2c35\u5929\uff0c\u901a\u8fc7\u5b9e\u65f6PCR\u5b9a\u91cf\u8840\u6e05HBV DNA\u6c34\u5e73\uff1b<\/p>\n

\uff08d-f\uff09\u5728\u4ece\u94c1\u86cb\u767dNP-preS1\u6216SC-preS1\u514d\u75ab\u5c0f\u9f20\u6216WT\u5c0f\u9f20\u6536\u96c6\u7684\u514d\u75ab\u8840\u6e05\u4e2d\uff0c\u5c06HepG2-hNTCP\u7ec6\u80de\u4e0e1\u00d7107<\/sup> vg HBV\u5b75\u80b2\u3002<\/p>\n

\u56fe\u4e09\u3001\u94c1\u86cb\u767dNP-preS1\u4f5c\u4e3a\u6cbb\u7597\u6027\u75ab\u82d7<\/strong>
\n \"\"\uff08a\uff09\u7ed9C57BL\/6\u5c0f\u9f20\u9759\u8109\u6ce8\u5c041\u00d71010<\/sup> vg AAV-HBV1.3\u75c5\u6bd2\u3002<\/p>\n

\uff08b-d\uff09\u5229\u7528ELISA\u6d4b\u5b9a\u8840\u6e05\u6297preS1\uff08b\uff09\u3001preS1\u6297\u539f\uff08c\uff09\u548cHBsAg\uff08d\uff09\u7684\u6c34\u5e73\uff1b<\/p>\n

\uff08e\uff09\u7b2c147\u5929\u65f6\uff0c\u6d4b\u5b9a\u8840\u6e05HBV DNA\u3001HBsAg\u548c\u6297HBs\u6c34\u5e73\uff1b<\/p>\n

\uff08f\uff09\u7b2c147\u5929\u65f6\uff0c\u5bf9\u809d\u7ec6\u80de\u4e2d\u4e59\u578b\u809d\u708e\u6838\u5fc3\u6297\u539f\uff08HBcAg\uff09\u7684\u514d\u75ab\u7ec4\u7ec7\u8fdb\u884c\u5316\u5b66\u67d3\u8272\uff1b<\/p>\n

\uff08g\uff09\u7b2c147\u5929\u65f6\uff0c\u5229\u7528\u5b9e\u65f6PCR\u5bf9\u809d\u810f\u4e2d\u7684HBV cccDNA\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\uff1b<\/p>\n

\uff08h\uff09\u7b2c147\u5929\u65f6\uff0c\u6d4b\u5f97\u6536\u96c6\u8840\u6e05\u4e2d\u7684\u8c37\u6c28\u9178-\u4e19\u916e\u9178\u8f6c\u6c28\u9176\uff08ALT\uff09\u548c\u8c37\u6c28\u9178-\u8349\u9170\u4e59\u9178\u8f6c\u6c28\u9176\uff08AST\uff09\u3002<\/p>\n

\u56fe\u56db\u3001\u94c1\u86cb\u767dNP\u75ab\u82d7\u9776\u5411\u4e0d\u540c\u7684APCs<\/strong>
\n \"\"\uff08a\uff09\u7ed9C57BL\/6\u5c0f\u9f20\u76ae\u4e0b\u6ce8\u5c042 nmol\u94c1\u86cb\u767dNP-eGFP\uff1b<\/p>\n

\uff08b-c\uff09C57BL\/6\u5c0f\u9f20\u76ae\u4e0b\u6ce8\u5c042 nmol\u94c1\u86cb\u767dNP-eGFP\u6216\u7b49\u6469\u5c14eGFP-SpyTag\uff1b<\/p>\n

\uff08d\uff0910\u5929\u540e\uff0c\u6ce8\u5c04CLL\u6216\u5bf9\u7167\u8102\u8d28\u4f53\uff08CON\uff09\uff0c\u5206\u6790DC\u6216CD11c-CD11b+<\/sup>\u5de8\u566c\u7ec6\u80de\u5bf9\u94c1\u86cb\u767dNP-eGFP\u7684\u6355\u83b7\uff1b<\/p>\n

\uff08e\uff09\u5728CLL\u6216CON\u6cbb\u759710\u5929\u540e\uff0c\u94c1\u86cb\u767dNP-eGFP\u5728pLNs\u4e2d\u84c4\u79ef2 h\u540e\uff0c\u901a\u8fc7\u514d\u75ab\u8367\u5149\u663e\u5fae\u955c\u786e\u5b9a\u6ce8\u5c04\u5242\u91cf\u3002<\/p>\n

\u56fe\u4e94\u3001\u94c1\u86cb\u767dNP\u75ab\u82d7\u8bf1\u5bfc\u6709\u6548\u7684Tfh\u548cGC\u53cd\u5e94<\/strong>
\n \"\"\uff08a\uff09\u75281\u00d7107<\/sup> Thy1.1+ OT-II\u813e\u7ec6\u80de\u8fc7\u7ee7\u8f6c\u79fb\u514d\u75ab\u7684Na\u00efve WT Thy1.2 C57BL\/6\u5c0f\u9f20\uff1b<\/p>\n

\uff08b\uff09\u5728\u514d\u75ab\u7684\u7b2c63\u5929\uff0c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u6765\u81eaiLN\u7684GC B\u7ec6\u80de\uff1b<\/p>\n

\uff08c\uff09\u7528\u6297-B220\u3001\u6297-GL7\u548cDAPI\u5bf9iLN\u7684\u51b7\u51bb\u5207\u7247\u67d3\u8272\uff1b<\/p>\n

\uff08d-e\uff09\u514d\u75ab\u63a5\u79cd12 h\u540e\uff0c\u5229\u7528rDC\uff08CD103–<\/sup>\uff09\u3001miDC\uff08CD103+<\/sup>\uff09\u3001CD169+<\/sup>\u5de8\u566c\u7ec6\u80de\uff08CD11c-CD11b+<\/sup>CD169+<\/sup>\uff09\u548cF4\/80+<\/sup>\u5de8\u566c\u7ec6\u80de\uff08CD11c-CD11b+<\/sup>CD169-F4\/80+<\/sup>\uff09dLNs\u4e0e\u672a\u7ecf\u5904\u7406\u7684OT-II T\u7ec6\u80de\u5171\u5b75\u80b2\uff1b<\/p>\n

\uff08f\uff09\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u8bc1\u5b9e\u5229\u7528DT\u5904\u7406\u7684CD11c-DTR\u9aa8\u9ad3\u5d4c\u5408\u5c0f\u9f20\u7684iLN\u4e2d\u7684DC\u6d88\u8017\uff1b<\/p>\n

\uff08g\uff09\u514d\u75ab\u540e\u7b2c10\u5929\uff0c\u5728CD11c\u7f3a\u5931\u7684\u5c0f\u9f20\u4e2d\u68c0\u6d4b\u5230PreS1\u7279\u5f02\u6027IgM\u548cIgG\u53cd\u5e94\u3002<\/p>\n

\u56fe\u516d\u3001B\u7ec6\u80de\u6fc0\u6d3b\u548c\u6297\u4f53\u4ea7\u751f\u9700\u8981\u7684SIGNR1+<\/sup>\u5de8\u566c\u7ec6\u80de<\/strong>
\n \"\"\uff08a\uff09\u5728f.p.\u7684\u7b2c3\u5929\u540e\uff0c\u4ecepLNs\u4e2d\u7b5b\u9009\u51faWT\u5c0f\u9f20\u4e2d\u7684\u514d\u75ab\u63a5\u79cd\uff0crDC\u3001miDC\u3001SIGNR1+<\/sup>\u5de8\u566c\u7ec6\u80de\u548cF4\/80+<\/sup>\u5de8\u566c\u7ec6\u80de\uff0c\u5e76\u4e0eMD4 B\u7ec6\u80de\u5b75\u80b2\uff1b<\/p>\n

\uff08b-d\uff09\u5728\u514d\u75ab\u94c1\u86cb\u767dNP-preS1\u75ab\u82d7\u4e4b\u524d\uff0c\u7528PBS\u6216\u6297CSF1R\u5904\u7406\u91ce\u751f\u578b\u5c0f\u9f20\uff1b<\/p>\n

\uff08e\uff09\u514d\u75ab\u540e\u7b2c3\u5929\uff0cWT\u548cCxcr5-\/-<\/sup>\u5c0f\u9f20\u7684iLN\u4e2dSIGNR1+<\/sup>\u7ec6\u80de\u7684\u5206\u5e03\uff1b<\/p>\n

\uff08f\uff09\u5728WT\uff1aCxcr5-\/-<\/sup>\u6df7\u5408\u9aa8\u9ad3\u5d4c\u5408\u5c0f\u9f20\u4e2d\u514d\u75abCpG-1826\u540e5 d\uff0ciLNs\u4e2dWT SIGNR1+<\/sup>\u7ec6\u80de\uff08CD45.1\uff09\u6216Cxcr5-\/-<\/sup>SIGNR1+<\/sup>\u7ec6\u80de\uff08CD45.2\uff09\u7684\u663e\u5fae\u955c\u89c2\u5bdf\uff1b<\/p>\n

\uff08g\uff09\u4ece\u6ce8\u5c04\u7684f.p.\u6ce8\u5c04\u7684WT\u6216Cxcr5-\/-<\/sup>\u5c0f\u9f20\u7684\u813e\u810f\u4e2d\u5206\u9009SIGNR1+<\/sup>\u5de8\u566c\u7ec6\u80de\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u672c\u6587\u7684\u6570\u636e\u8868\u660e\uff0c\u5728\u5c0f\u9f20HBV\u6a21\u578b\u4e2d\uff0c\u901a\u8fc7\u534f\u8c03\u53cc\u9776\u5411SIGNR1+<\/sup>\u6811\u7a81\u72b6\u7ec6\u80de\u548cLNs\u4e2d\u7684\u5de8\u566c\u7ec6\u80de\uff0c\u57fa\u4e8e\u94c1\u86cb\u767dNP\u7684preS1\u75ab\u82d7\u8868\u73b0\u51fa\u9ad8\u6548\u7684\u6297\u4f53\u5e94\u7b54\uff0c\u65e2\u5177\u6709\u9884\u9632\u4f5c\u7528\uff0c\u53c8\u5177\u6709\u6cbb\u7597\u4f5c\u7528\u3002\u8be5\u7814\u7a76\u53ef\u80fd\u4e3a\u4eca\u540eCHB\u6cbb\u7597\u7684\u8f6c\u5316\u7814\u7a76\u63d0\u4f9b\u4e00\u4e2a\u826f\u597d\u7684\u9009\u62e9\u548c\u65b0\u601d\u8def\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aDual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B<\/a><\/strong>\uff08Nature Nanotechnology<\/em>, 2020<\/strong>, DOI: 10.1038\/s41565-020-0648-y\uff09<\/p>\n

\u901a\u8baf\u4f5c\u8005\u7b80\u4ecb<\/strong><\/p>\n

\u6731\u660e\u662d\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\uff0c\u611f\u67d3\u4e0e\u514d\u75ab\u9662\u91cd\u70b9\u5b9e\u9a8c\u5ba4\uff0c\u7814\u7a76\u5458\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u5927\u5b66\u5c97\u4f4d\u6559\u6388\uff0c\u535a\u58eb\u751f\u5bfc\u5e08\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u201c\u767e\u4eba\u8ba1\u5212\u201d\u5f15\u8fdb\u6d77\u5916\u6770\u51fa\u4eba\u624d<\/strong>\u3002\u4e3b\u8981\u4ece\u4e8b\u6dcb\u5df4\u7ec4\u7ec7\u5fae\u73af\u5883\u548cT\u7ec6\u80de\u53d1\u80b2\u548c\u5e94\u7b54\uff0c\u75ab\u82d7\u514d\u75ab\u5b66\u7b49\u65b9\u9762\u7684\u7814\u7a76\u3002\u4e00\u65b9\u9762\uff0c\u63ed\u793a\u4e86\u6dcb\u5df4\u7ec4\u7ec7\u5fae\u73af\u5883\u591a\u79cd\u57fa\u8d28\u7ec6\u80de\u53d1\u80b2\u548c\u529f\u80fd\u7684\u8c03\u63a7\u673a\u5236\uff0c\u63ed\u793a\u4e86\u6dcb\u5df4\u7ec4\u7ec7\u5fae\u73af\u5883\u5bf9T\u7ec6\u80de\u53d1\u80b2\u548c\u5e94\u7b54\u7684\u5f71\u54cd\u548c\u4f5c\u7528\u673a\u5236\uff0c\u63ed\u793a\u4e86T\u7ec6\u80de\u53d1\u80b2\u548c\u5e94\u7b54\u7684\u8868\u89c2\u9057\u4f20\u8c03\u63a7\u673a\u5236\uff1b\u53e6\u4e00\u65b9\u9762\uff0c\u5f00\u53d1\u4e86\u57fa\u4e8eSpyTag\/SpyCatcher\u7684\u70b9\u51fb\u75ab\u82d7\u6280\u672f\uff0c\u8bbe\u8ba1\u4e86\u4e00\u7cfb\u5217\u57fa\u4e8e\u7eb3\u7c73\u80f6\u675f\u548c\u94c1\u86cb\u767d\u7eb3\u7c73\u9897\u7c92\u7b49\u8f7d\u4f53\u3001\u9776\u5411\u6dcb\u5df4\u7ed3\u548c\u6297\u539f\u5448\u9012\u7ec6\u80de\u7684\u65b0\u578b\u70b9\u51fb\u75ab\u82d7\u548c\u7eb3\u7c73\u75ab\u82d7\uff0c\u5e94\u7528\u4e8e\u80bf\u7624\u548c\u4f20\u67d3\u75c5\u7684\u514d\u75ab\u9884\u9632\u548c\u6cbb\u7597\u7814\u7a76\u3002\u7814\u7a76\u6210\u679c\u53d1\u8868\u4e8eNature, Nature Nanotechnology\uff0cNature Communications, Cell Reports, Cell Discovery, Journal of Immunology, Oncoimmunology, Nanomedicine\u7b49\u6742\u5fd7\u3002<\/p>\n

\u672c\u6587\u7531CQR\u7f16\u8bd1\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com.<\/p>\n

\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenvip\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011 \u4e59\u578b\u809d\u708e\uff08CHB\uff09\u662f\u7531\u4e59\u578b\u809d\u708e\u75c5\u6bd2\uff08HBV\uff09\u611f\u67d3\u5f15\u8d77\u7684\u5168\u7403\u6027\u5065\u5eb7\u95ee\u9898\uff0cHBV\u4f1a\u653b\u51fb\u809d\u810f\uff0c\u4ece\u800c\u5bfc\u81f4\u6025\u6027\u548c\u6162\u6027\u75be\u75c5\u3002\u636e\u4f30\u8ba1\uff0c\u76ee\u524d\u5168\u7403\u67092.57\u4ebf\u4eba\u662fH…<\/p>\n","protected":false},"author":7770,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[4342],"class_list":["post-198607","post","type-post","status-publish","format-standard","hentry","category-jiedu","tag-nppres1"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/198607"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/7770"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=198607"}],"version-history":[{"count":5,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/198607\/revisions"}],"predecessor-version":[{"id":199289,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/198607\/revisions\/199289"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=198607"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=198607"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=198607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}