{"id":207320,"date":"2020-08-13T06:41:26","date_gmt":"2020-08-12T22:41:26","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=207320"},"modified":"2020-08-12T21:41:51","modified_gmt":"2020-08-12T13:41:51","slug":"%e6%b5%99%e5%a4%a7-acs-nano%ef%bc%9a180%e5%ba%a6%e5%a4%a7%e5%8f%8d%e8%bd%ac%ef%bc%81%e6%94%b9%e5%8f%98%e8%87%aa%e5%99%ac%e4%bd%9c%e7%94%a8%e6%8f%90%e9%ab%98%e5%af%b9%e4%b9%8f%e6%b0%a7%e8%82%bf","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=207320","title":{"rendered":"\u6d59\u5927 ACS Nano\uff1a180\u5ea6\u5927\u53cd\u8f6c\uff01\u6539\u53d8\u81ea\u566c\u4f5c\u7528\u63d0\u9ad8\u5bf9\u4e4f\u6c27\u80bf\u7624\u7684\u5149\u52a8\u529b\u6cbb\u7597"},"content":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011<\/strong><\/p>\n

\u5149\u52a8\u529b\u6cbb\u7597\uff08PDT\uff09<\/strong>\u662f\u4e00\u79cd\u7528\u4e8e\u8bb8\u591a\u5c40\u90e8\u548c\u8868\u9762\u75be\u75c5\u7684\u975e\u4fb5\u88ad\u6cbb\u7597\u65b0\u65b9\u6cd5\u3002\u5728PDT\u8fc7\u7a0b\u4e2d\uff0c\u4f1a\u4ea7\u751f\u5177\u6709\u7ec6\u80de\u6bd2\u6027\u7684\u6d3b\u6027\u6c27\uff08ROS\uff09\u6765\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u3002\u540c\u65f6\uff0cROS\u4e5f\u662f\u81ea\u566c\u7684\u91cd\u8981\u8bf1\u56e0\u3002\u5176\u5b9e\uff0c\u81ea\u566c\u662f\u4e00\u79cd\u5206\u89e3\u4ee3\u8c22\u8fc7\u7a0b\uff0c\u901a\u8fc7\u6eb6\u9176\u4f53\u9014\u5f84\u964d\u89e3\u529f\u80fd\u5931\u8c03\u6216\u6709\u5bb3\u7684\u7ec6\u80de\u5668\u548c\u86cb\u767d\u8d28\u3002\u4f46\u662f\uff0c\u8fc7\u5ea6\u7684\u6fc0\u6d3b\u81ea\u566c\u4f1a\u5bfc\u81f4\u81ea\u566c\u7ec6\u80de\u6b7b\u4ea1\u3002\u56e0\u6b64\uff0c\u81ea\u566c\u4e0e\u7ec6\u80de\u51cb\u4ea1\u4e4b\u95f4\u7684\u4e32\u6270\u5bf9PDT\u7684\u6cbb\u7597\u6548\u679c\u6709\u91cd\u8981\u5f71\u54cd\u3002\u7136\u800c\uff0c\u5229\u7528\u81ea\u566c\u6291\u5236\u5242\u6765\u5b8c\u5168\u6291\u5236\u81ea\u566c\u975e\u5e38\u56f0\u96be\u3002\u56e0\u6b64\uff0c\u5408\u7406\u8bbe\u8ba1\u7eb3\u7c73\u6750\u6599\u6765\u8c03\u63a7\u81ea\u566c\u4ece\u4fc3\u751f\u5b58\u53d8\u6210\u4fc3\u6b7b\u4ea1\uff0c\u5c06\u662fPDT\u4e2d\u4e00\u4e2a\u6709\u610f\u4e49\u7684\u7814\u7a76\u65b9\u5411\u3002\u4f17\u6240\u5468\u77e5\uff0c\u7f3a\u6c27\u662f\u8bb8\u591a\u5b9e\u4f53\u7624\u7684\u663e\u7740\u7279\u5f81\u3002\u56e0\u6b64\uff0c\u5982\u4f55\u7f13\u89e3\u80bf\u7624\u7f3a\u6c27\u662fPDT\u7684\u91cd\u4e2d\u4e4b\u91cd\u3002\u76ee\u524d\uff0c\u5927\u591a\u6570\u7814\u7a76\u96c6\u4e2d\u5728\u901a\u8fc7\u5c06H2<\/sub>O2<\/sub>\u5206\u89e3\u4e3aO2<\/sub>\u6216\u76f4\u63a5\u8f93\u9001O2<\/sub>\u6765\u589e\u52a0O2<\/sub>\u7684\u4f9b\u5e94\u3002\u5176\u5b9e\uff0c\u901a\u8fc7\u51cf\u5c11\u751f\u7406O2<\/sub>\u7684\u6d88\u8017\u4e5f\u662f\u4e00\u79cd\u7f13\u89e3\u80bf\u7624\u7f3a\u6c27\u7684\u65b9\u6cd5\u3002\u4f46\u662f\uff0c\u5982\u4f55\u901a\u8fc7\u6291\u5236O2<\/sub>\u7684\u6d88\u8017\u6765\u7f13\u89e3\u80bf\u7624\u7f3a\u6c27\u7684\u65b9\u6cd5\u4ecd\u672a\u5f97\u5230\u5145\u5206\u7684\u63a2\u7d22\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u6d59\u6c5f\u5927\u5b66\u7684\u91d1\u6865\u526f\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09\u548c\u8ba1\u5251\u6559\u6388\u7b49\u4eba<\/strong>\u62a5\u9053\u4e86\u4e00\u79cd\u65b0\u578b\u7684\u8d85\u5206\u5b50\u7eb3\u7c73\u5e73\u53f0\uff0c\u901a\u8fc7\u5c06\u81ea\u566c\u7684\u4f5c\u7528\u4ece\u4fc3\u8fdb\u751f\u5b58\u8f6c\u53d8\u4e3a\u4fc3\u8fdb\u6b7b\u4ea1\u6765\u589e\u5f3aPDT\u3002\u5229\u7528\u547c\u5438\u6291\u5236\u52423-\u6eb4\u4e19\u916e\u9178\uff083BP\uff09\u4f5c\u4e3a\u81ea\u566c\u542f\u52a8\u56e0\u5b50\u548c\u7f3a\u6c27\u6539\u5584\u5242\uff0c\u5c06\u5176\u6574\u5408\u5230\u5149\u654f\u5242Chlorin e6\uff08Ce6\uff09\u5305\u88f9\u7684\u7eb3\u7c73\u9897\u7c92\u4e2d\uff0c\u4ee5\u5bf9\u6297\u7f3a\u6c27\u80bf\u7624\u3002\u5176\u4e2d\uff0c3BP\u901a\u8fc7\u4e0b\u8c03HK-II\u548cGAPDH\u7684\u8868\u8fbe\u6765\u6291\u5236\u547c\u5438\u4f5c\u7528\uff0c\u4ece\u800c\u663e\u7740\u964d\u4f4e\u7ec6\u80de\u5185\u8017\u6c27\u7387\uff0c\u4ee5\u51cf\u8f7b\u80bf\u7624\u7684\u7f3a\u6c27\u800c\u589e\u5f3aPDT\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u901a\u8fc7Western\u5370\u8ff9\u3001\u514d\u75ab\u8367\u5149\u6210\u50cf\u548c\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\uff08TEM\uff09\u6d4b\u5b9a\uff0c\u53d1\u73b03BP\u548cPDT\u7684\u7ed3\u5408\u663e\u8457\u63d0\u9ad8\u4e86\u81ea\u566c\u7684\u6c34\u5e73\u3002\u8fc7\u5ea6\u6fc0\u6d3b\u7684\u81ea\u566c\u4f5c\u7528\u4fc3\u8fdb\u4e86\u7ec6\u80de\u7684\u51cb\u4ea1\uff0c\u56e0\u6b64\u7ed3\u54083BP\u7684PDT\u53ef\u4ee5\u6709\u6548\u6291\u5236\u7ec6\u80de\u589e\u6b96\uff0c\u540c\u65f6\u6740\u6b7b\u80bf\u7624\u3002\u6b64\u5916\uff0c\u901a\u8fc73BP\u51cf\u8f7b\u80bf\u7624\u7f3a\u6c27\u540e\uff0c\u53ef\u964d\u4f4e\u7f3a\u6c27\u8bf1\u5bfc\u56e0\u5b501\u03b1\uff08HIF-1\u03b1\uff09\u7684\u8868\u8fbe\uff0c\u8fdb\u800c\u901a\u8fc7\u6d88\u9664\u539f\u53d1\u80bf\u7624\u548c\u4e0b\u8c03HIF-1\u03b1\u8868\u8fbe\u6765\u6709\u6548\u6291\u5236\u80bf\u7624\u7684\u8f6c\u79fb\u3002\u603b\u4e4b\uff0c\u8fd9\u4e9b\u7ed3\u679c\u4e3a\u672a\u6765\u7684\u764c\u75c7\u6cbb\u7597\u521b\u65b0\u65b9\u6cd5\u63d0\u4f9b\u4e86\u65b0\u601d\u8def\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a\u201c3-Bromopyruvate-Conjugated Nanoplatform-Induced Pro-Death Autophagy for Enhanced Photodynamic Therapy against Hypoxic Tumor\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520aACS Nano<\/strong>\u4e0a\u3002
\n \"\"<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/strong><\/p>\n

\u56fe\u4e00\u3001CD-Ce6-3BP NPs\u548cCD-Ce6 NPs\u7684\u8868\u5f81<\/strong>
\n \"\"\uff08A\uff09\u5177\u6709\u4e0d\u540c\u8d28\u91cf\u6bd4\u76843BP\u4e0eCe6\u7684CD-Ce6-3BP NPs\u7684\u7c92\u5f84\u5c3a\u5bf8\uff1b<\/p>\n

\uff08B\uff09CD-Ce6-3BP NPs\u548cCD-Ce6 NPs\u7684Dh\u548cTEM\u56fe\u50cf\uff1b<\/p>\n

\uff08C\uff09\u5728\u50a8\u5b5810 d\u671f\u95f4\uff0cPBS\u7f13\u51b2\u6db2\u4e2d\u4e0d\u540c\u7eb3\u7c73\u9897\u7c92\u7684Dh\uff1b<\/p>\n

\uff08D\uff09\u5229\u7528660 nm\u7167\u5c04\u5904\u7406\u6216pH=5.5\u6eb6\u6db2\u5904\u74064 h\u540e\uff0cCD-Ce6-3BP NPs\u7684D<\/em>h<\/sub>\u53d8\u5316\u3002<\/p>\n

\u56fe\u4e8c\u3001\u7cd6\u9175\u89e3\u6291\u5236\u3001O2<\/sub>\u6d88\u8017\u51cf\u5c11\u548cCD-Ce6-3BP NPs\u7684\u4f53\u5916\u7ec6\u80de\u6bd2\u6027\u4fc3\u8fdb\u4f5c\u7528<\/strong>
\n \"\"\uff08A\uff09KB\u7ec6\u80de\u4e0e\u4e0d\u540c\u5904\u7406\u5b75\u80b26 h\u540e\uff0cWestern blot\u68c0\u6d4bHK-II\u3001GAPDH\u548cHIF-1\u03b1\u7684\u6c34\u5e73\uff1b<\/p>\n

\uff08B\uff09KB\u7ec6\u80de\u4e0e\u4e0d\u540c\u5904\u7406\u5b75\u80b26 h\u540e\uff0c\u5b9a\u91cf\u5206\u6790HK-\u2161\u7684\u76f8\u5bf9\u8868\u8fbe\uff1b<\/p>\n

\uff08C\uff09KB\u7ec6\u80de\u4e0e\u4e0d\u540c\u5904\u7406\u5b75\u80b26 h\u540e\uff0c\u5b9a\u91cf\u5206\u6790GAPDH\u7684\u76f8\u5bf9\u8868\u8fbe\uff1b<\/p>\n

\uff08D\uff09KB\u7ec6\u80de\u4e0eCD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5b75\u80b26 h\u540e\uff0c\u7ec6\u80de\u5185\u4e73\u9178\u6c34\u5e73\uff1b<\/p>\n

\uff08E\uff09KB\u7ec6\u80de\u4e0eCD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5b75\u80b26 h\u540e\uff0c\u7ec6\u80de\u5185ATP\u6c34\u5e73\uff1b<\/p>\n

\uff08F\uff09\u7528\u4e0d\u540c\u5904\u7406\u5b75\u80b26 h\u540e\uff0cKB\u7ec6\u80de\u7684\u8017\u6c27\u91cf\uff1b<\/p>\n

\uff08G\uff09KB\u7ec6\u80de\u4e0e\u4e0d\u540c\u5904\u7406\u5b75\u80b26 h\u540e\uff0c\u5b9a\u91cf\u5206\u6790HIF-1\u03b1\u7684\u76f8\u5bf9\u8868\u8fbe\uff1b<\/p>\n

\uff08H\uff09\u7528CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u3001CD-Ce6-3BP NPs\/L\u3001\u6e38\u79bb3BP\u6216CD-3BP\u5904\u7406\u540e\uff0cKB\u7ec6\u80de\u7684\u5b58\u6d3b\u7387\uff1b<\/p>\n

\uff08I\uff09\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5229\u7528\u8367\u5149\u63a2\u9488\u68c0\u6d4b\u7684\u7ec6\u80de\u6bd2\u6027\u3002<\/p>\n

\u56fe\u4e09\u3001CD-Ce6-3BP NPs\u7684\u81ea\u566c\u4f5c\u7528\u4fc3\u8fdb\u7ec6\u80de\u6b7b\u4ea1<\/strong>
\n \"\"\uff08A\uff09CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u3001CD-Ce6-3BP NPs\/L\u3001\u6e38\u79bb3BP\u6216CD-3BP\u5904\u7406KB\u7ec6\u80de6 h\u540e\uff0cWestern blot \u68c0\u6d4bLC3-I\u3001LC3-II\u548cp62\u7684\u6c34\u5e73\uff1b<\/p>\n

\uff08B\uff09\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5b9a\u91cf\u5206\u6790LC3-II\/LC3-I\uff1b<\/p>\n

\uff08C\uff09\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5b9a\u91cf\u5206\u6790p62\u7684\u76f8\u5bf9\u8868\u8fbe\uff1b<\/p>\n

\uff08D\uff09\u6709\u65e0\u6fc0\u5149\u7167\u5c041 min\u7684\u60c5\u51b5\u4e0b\uff0c\u7528CD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5904\u7406KB\u7ec6\u80de4 h\u540e\uff0c\u4ee3\u8868\u6027LC3\u70b9\u72b6\u70b9\u7684\u514d\u75ab\u8367\u5149\u56fe\u50cf\uff1b<\/p>\n

\uff08E\uff09\u6709\u65e0\u6fc0\u5149\u7167\u5c041 min\u7684\u60c5\u51b5\u4e0b\uff0c\u7528CD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5904\u7406KB\u7ec6\u80de4 h\u540e\uff0cMDC\u67d3\u8272\u7684\u8367\u5149\u56fe\u50cf\uff1b<\/p>\n

\uff08F\uff09\u6709\u65e0\u6fc0\u5149\u7167\u5c041 min\u7684\u60c5\u51b5\u4e0b\uff0c\u7528CD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5904\u7406KB\u7ec6\u80de4 h\u540e\uff0c\u5f62\u6210\u81ea\u566c\u4f53\u7684TEM\u56fe\uff1b<\/p>\n

\uff08G\uff09\uff08F\uff09\u4e2d\u653e\u5927\u500d\u6570\u7684TEM\u56fe\u50cf\uff1b<\/p>\n

\uff08H\uff09\u5728\u5b58\u5728RAPA\u62163-MA\u7684\u60c5\u51b5\u4e0b\uff0c\u7528CD-Ce6 NPs\/L\u6216CD-Ce6-3BP NPs\/L\u5904\u7406\u540e\uff0cKB\u7ec6\u80de\u7684\u5b58\u6d3b\u7387\uff1b<\/p>\n

\uff08I\uff09\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5229\u7528\u8367\u5149\u63a2\u9488\u68c0\u6d4b\u7684\u7ec6\u80de\u6bd2\u6027\u3002<\/p>\n

\u56fe\u56db\u3001CD-Ce6-3BP NPs\u589e\u52a0\u51cb\u4ea1\u6c34\u5e73<\/strong>
\n \"\"\uff08A\uff09\u7528CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u3001CDCe6-3BP NPs\/L\u3001\u6e38\u79bb3BP\u6216CD-3BP\u5904\u7406KB\u7ec6\u80de6 h\u540e\uff0cWestern blot\u68c0\u6d4bcaspase 3\u7684\u8868\u8fbe\uff1b<\/p>\n

\uff08B\uff09\u5229\u7528Annexin V-FITC\u548cPI\u5206\u6790\u6cd5\u5bf9CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u6216CD-Ce6-3BP NPs\/L\u5904\u7406KB\u7ec6\u80de\u540e\uff0c\u5bf9\u51cb\u4ea1\u7ec6\u80de\u8fdb\u884c\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u3002<\/p>\n

\u56fe\u4e94\u3001\u5bf9\u76ae\u4e0b\u80bf\u7624\u8fdb\u884c\u4f53\u5185\u6297\u80bf\u7624\u3001\u81ea\u566c\u548c\u51cb\u4ea1\u6c34\u5e73\u8bc4\u4f30<\/strong>
\n \"\"\uff08A\uff09\u6e38\u79bbCe6\u3001CD-Ce6\u548cCD-Ce6-3BP NPs\u7684\u836f\u4ee3\u52a8\u529b\u5b66\u7279\u5f81\uff1b<\/p>\n

\uff08B\uff09\u7f3a\u6c27\u548c\u8840\u7ba1\u67d3\u8272\u540e\u80bf\u7624\u5207\u7247\u7684\u4ee3\u8868\u6027\u514d\u75ab\u8367\u5149\u56fe\u50cf\uff1b<\/p>\n

\uff08C\uff09CD-Ce6 NPs\u6216CD-Ce6-3BP NPs\u5904\u7406\u540e\uff0cWestern blot\u68c0\u6d4b\u5c0f\u9f20\u80bf\u7624\u7ec4\u7ec7\u4e2dHIF-1\u03b1\u7684\u6c34\u5e73\uff1b<\/p>\n

\uff08D\uff09\u6839\u636e\uff08C\uff09\u7684\u5c0f\u9f20\u80bf\u7624\u7ec4\u7ec7\uff0c\u5b9a\u91cf\u5206\u6790HIF-1\u03b1\u7684\u76f8\u5bf9\u8868\u8fbe\uff1b<\/p>\n

\uff08E\uff09KB\u76ae\u4e0b\u80bf\u7624\u63a5\u79cd\u3001\u7ed9\u836f\u6cbb\u7597\u65b9\u6848\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08F\uff09PBS\u3001CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CDCe6 NPs\/L\u548cCD-Ce6-3BP NPs\/L\u5904\u7406\u540e\uff0c\u5e26\u6709\u76ae\u4e0bKB\u80bf\u7624\u7684\u88f8\u9f20\u7684\u80bf\u7624\u751f\u957f\u66f2\u7ebf\uff1b<\/p>\n

\uff08G\uff09\u6bcf\u53ea\u60a3\u6709\u76ae\u4e0bKB\u80bf\u7624\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u91cd\u91cf\uff1b<\/p>\n

\uff08H\uff09\u4e0d\u540c\u5904\u7406\u540e\uff0cWestern blot\u68c0\u6d4b\u5c0f\u9f20\u80bf\u7624\u7ec4\u7ec7\u4e2dLC3-\u2160\u3001LC3-II\u3001p62\u548ccaspase 3\u7684\u6c34\u5e73\uff1b<\/p>\n

\uff08I\uff09PBS\u3001CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u548cCDCe6-3BP NPs\/L\u5904\u7406\u540e\uff0c\u5c0f\u9f20\u80bf\u7624\u7ec4\u7ec7\u4e2dLC3\u7684\u4ee3\u8868\u6027\u514d\u75ab\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

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\uff08B\uff09PBS\u3001CD-Ce6 NPs\u3001CD-Ce6-3BP NPs\u3001CD-Ce6 NPs\/L\u548cCD-Ce6-3BP NPs\/L\u5904\u7406KB\u80bf\u7624\u88f8\u9f20\u540e\u7684\u80bf\u7624\u751f\u957f\u66f2\u7ebf\uff1b<\/p>\n

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\uff08D\uff09\u6ce8\u5c04\u540e21 d\u540e\uff0c\u4ece\u5c0f\u9f20\u6536\u83b7\u7684\u539f\u4f4d\u80bf\u7624\u7684\u80bf\u7624\u56fe\u50cf\uff1b<\/p>\n

\uff08E\uff094T1\u80ba\u8f6c\u79fb\u5c0f\u9f20\u7684\u751f\u7269\u53d1\u5149\u6210\u50cf\u7167\u7247\uff1b<\/p>\n

\uff08F\uff09\u5904\u740621 d\u540e\uff0c\u80ba\u90e8\u7684\u751f\u7269\u53d1\u5149\u6210\u50cf\u7167\u7247\uff1b<\/p>\n

\uff08G\uff09\u5404\u7ec4\u80ba\u7684\u751f\u7269\u53d1\u5149\u5f3a\u5ea6\uff1b<\/p>\n

\uff08H\uff09\u6bcf\u7ec4\u80ba\u8868\u9762\u4e0a\u5b58\u5728\u7684\u80bf\u7624\u7ed3\u8282\u7684\u6570\u76ee\uff1b<\/p>\n

\uff08I\uff09\u5404\u7ec4\u80ba\u4e2d\u7684\u80bf\u7624\u8986\u76d6\u7387\uff1b<\/p>\n

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\u56fe\u4e03\u3001\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/strong>
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\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

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\u6587\u732e\u94fe\u63a5\uff1a3-Bromopyruvate-Conjugated Nanoplatform-Induced Pro-Death Autophagy for Enhanced Photodynamic Therapy against Hypoxic Tumor<\/a><\/strong>\uff08ACS Nano<\/em>, 2020<\/strong>, DOI: 10.1021\/acsnano.0c01350\uff09<\/p>\n

\u672c\u6587\u7531CQR\u7f16\u8bd1\u3002<\/p>\n

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