{"id":218339,"date":"2021-02-28T06:02:02","date_gmt":"2021-02-27T22:02:02","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=218339"},"modified":"2021-02-27T19:02:14","modified_gmt":"2021-02-27T11:02:14","slug":"%e4%b8%ad%e7%a7%91%e9%99%a2%e8%8b%8f%e5%b7%9e%e7%ba%b3%e7%b1%b3%e6%89%80%e7%8e%8b%e5%bc%ba%e6%96%8c%e5%9b%a2%e9%98%9fnano-lett-%ef%bc%9a%e5%85%b7%e6%9c%89%e5%8f%af%e8%b0%83%e7%bb%93%e6%9e%84%e7%9a%84","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=218339","title":{"rendered":"\u4e2d\u79d1\u9662\u82cf\u5dde\u7eb3\u7c73\u6240\u738b\u5f3a\u658c\u56e2\u961fNano Lett.\uff1a\u5177\u6709\u53ef\u8c03\u7ed3\u6784\u7684\u86cb\u767d\u8d28\u6676\u4f53\u7684\u975e\u5171\u4ef7\u81ea\u7ec4\u88c5"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/b><\/strong><\/p>\n

\u64cd\u63a7\u7eb3\u7c73\u7ea7\u6784\u7b51\u57fa\u5143\u7ec4\u88c5\u6210\u9ad8\u5ea6\u6709\u5e8f\u7684\u6676\u4f53\u7ed3\u6784\u5df2\u7ecf\u5438\u5f15\u4e86\u5927\u91cf\u7684\u7814\u7a76\u5174\u8da3\u3002\u901a\u8fc7\u4f18\u5316\u65e0\u673a\u7eb3\u7c73\u9897\u7c92\u7684\u7c7b\u578b\u3001\u5c3a\u5bf8\u3001\u6676\u683c\u53c2\u6570\u548c\u6676\u4f53\u5bf9\u79f0\u6027\uff0c\u53ef\u4ee5\u5728\u70ed\u529b\u5b66\u6216\u52a8\u529b\u5b66\u4e0a\u8c03\u63a7\u5176\u81ea\u7ec4\u88c5\u8fc7\u7a0b\uff0c\u8fdb\u800c\u83b7\u5f97\u4e00\u5143\u3001\u4e8c\u5143\u548c\u4e09\u5143\u7684\u6676\u4f53\u7ed3\u6784\u3002\u6e90\u4e8e\u9ad8\u5ea6\u6709\u5e8f\u6392\u5217\u6240\u4ea7\u751f\u7684\u65b0\u5174\u7279\u6027\u548c\u591a\u6837\u7684\u5316\u5b66\u529f\u80fd\uff0c\u8fd9\u4e9b\u81ea\u7ec4\u88c5\u7ed3\u6784\u5728\u50ac\u5316\u3001\u78c1\u5b66\u3001\u5149\u5b66\u548c\u529f\u80fd\u751f\u7269\u6750\u6599\u7b49\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\u524d\u666f\u3002\u4ee5\u529f\u80fd\u86cb\u767d\u4e3a\u57fa\u5143\u7ed3\u6784\u7cbe\u786e\u6784\u5efa\u7ed3\u6784\u53ef\u8c03\u7684\u5468\u671f\u9635\u5217\u662f\u4e00\u4e2a\u975e\u5e38\u6709\u8da3\u7684\u7814\u7a76\u65b9\u5411\uff0c\u7136\u800c\uff0c\u86cb\u767d\u8d28\u8868\u9762\u7684\u5316\u5b66\u548c\u7ed3\u6784\u590d\u6742\u6027\uff0c\u7279\u522b\u662f\u5bf9\u4e8e\u975e\u7403\u5f62\u86cb\u767d\u8d28\u6a21\u5757\u800c\u8a00\uff0c\u5982\u4f55\u5c06\u5404\u5411\u5f02\u6027\u7684\u86cb\u767d\u8d28\u57fa\u5143\u53ef\u63a7\u7ec4\u88c5\u6210\u5177\u6709\u53ef\u8c03\u7ed3\u6784\u7279\u6027\u7684\u86cb\u767d\u8d28\u6676\u4f53\u4ecd\u7136\u662f\u4e00\u4e2a\u4e3b\u8981\u6311\u6218\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/b><\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u82cf\u5dde\u7eb3\u7c73\u6280\u672f\u4e0e\u7eb3\u7c73\u4eff\u751f\u7814\u7a76\u6240\u738b\u5f3a\u658c\u7814\u7a76\u5458\u56e2\u961f<\/b><\/strong>\u5229\u7528<\/b><\/strong>\u4e00\u79cd\u5404\u5411\u5f02\u6027\u7684L-\u9f20\u674e\u6811\u80f6\u7cd6-1-\u78f7\u9178\u919b\u7f29\u9176 (RhuA)\u4f5c\u4e3a\u6784\u7b51\u57fa\u5143\uff0c\u901a\u8fc7\u5728\u86cb\u767d\u8d28\u4e09\u7ef4\uff083D\uff09\u7a7a\u95f4\u4f4d\u70b9\u5f15\u5165\u7ec4\u6c28\u9178\u6b8b\u57fa\u5e76\u4e14\u7cbe\u786e\u8c03\u63a7\u5176\u76f8\u4e92\u4f5c\u7528\uff0c\u6210\u529f\u5b9e\u73b0\u4e86\u5c06\u5404\u5411\u5f02\u6027\u7684\u975e\u7403\u5f62RhuA\u86cb\u767d\u8d28\u81ea\u7ec4\u88c5\u6210\u9ad8\u5ea6\u6709\u5e8f\u7684\u3001\u5177\u6709\u4e0d\u540c\u5f62\u6001\u548c\u7ed3\u6784\u7684\u86cb\u767d\u8d28\u6676\u4f53\u3002\u57fa\u4e8e\u7ec4\u6c28\u9178\u7684\u03c0 -\u03c0\u4f5c\u7528\uff0c\u901a\u8fc7\u70ed\u529b\u5b66\u8c03\u63a7RhuA\u7684\u81ea\u7ec4\u88c5\u8fc7\u7a0b\uff0c\u83b7\u5f97\u4e863D\u7eb3\u7c73\u5e26\u548c3D\u56db\u65b9\u76f8\u6676\u4f53\u7ed3\u6784\uff1b\u901a\u8fc7\u5f15\u5165\u5f3a\u7684\u91d1\u5c5e\u79bb\u5b50-\u7ec4\u6c28\u9178\u87af\u5408\u4f5c\u7528\uff0c\u4ece\u52a8\u529b\u5b66\u4e0a\u8c03\u63a7\u4e86RhuA\u81ea\u7ec4\u88c5\u62103D\u56db\u65b9\u76f8\u6676\u4f53\u7ed3\u6784\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u5728RhuA\u6a21\u5757\u8868\u9762\u4e0a\u8bbe\u8ba1\u53e6\u4e00\u79cd\u7ec4\u6c28\u9178\u7684\u6392\u5e03\uff0c\u8fdb\u4e00\u6b65\u6784\u5efa\u4e86RhuA\u86cb\u767d\u8d28\u53cc\u87ba\u65cb3D\u8d85\u7ed3\u6784\u3002\u5229\u7528\u8367\u5149\u663e\u5fae\u955c\u3001\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\u00a0(TEM)\u3001\u539f\u5b50\u529b\u663e\u5fae\u955c(AFM) \u548c\u5c0f\u89d2\u5ea6X\u5c04\u7ebf\u6563\u5c04\u00a0(SAXS) \u6d4b\u5b9a\u4e86\u5b83\u4eec\u7684\u7ed3\u6784\u6027\u8d28\u548c\u52a8\u6001\u7ec4\u88c5\u6027\u8d28\u3002\u8fd9\u9879\u5de5\u4f5c\u65e8\u5728\u62d3\u5c55\u53ef\u8c03\u63a7\u3001\u9ad8\u5ea6\u6709\u5e8f\u7684\u86cb\u767d\u8d28\u8d85\u7ed3\u6784\u53ef\u7f16\u7a0b\u7684\u5de5\u5177\u76d2\uff0c\u5e76\u4e14\u6709\u52a9\u4e8e\u86cb\u767d\u8d28\u754c\u9762\u76f8\u4e92\u4f5c\u7528\u673a\u5236\u7684\u7406\u89e3\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a\u201c<\/b><\/strong>Noncovalent Self-Assembly of Protein Crystals with Tunable Structures<\/b><\/strong>\u201d<\/b><\/strong>\u53d1\u8868\u5728\u4e86Nano Lett.<\/b><\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n

\u56fe1. <\/b><\/strong>3D\u7eb3\u7c73\u5e26\u30013D\u86cb\u767d\u8d28\u6676\u4f53\u548c\u53cc\u87ba\u65cb\u86cb\u767d\u8d28\u8d85\u7ed3\u6784\u5f62\u6210\u7684\u793a\u610f\u56fe<\/b><\/strong>\"\"<\/p>\n

(A)\u00a0\u8bbe\u8ba1H47<\/sup>RhuA-C(2H)\u00a0\u53d8\u4f53\uff0c\u5728\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u7684\u4ecb\u5bfc\u4e0b\u81ea\u7ec4\u88c5\u5f62\u62103D\u7eb3\u7c73\u5e26\u548c\u4e24\u79cd\u7c7b\u578b3D\u6676\u4f53\u7ed3\u6784\u3002<\/p>\n

(B)\u8bbe\u8ba1H47\/H157<\/sup>RhuA-C(2H)-N(2H)\u00a0\u53d8\u4f53\uff0c\u5728\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u4ecb\u5bfc\u4e0b\u81ea\u7ec4\u88c5\u5f62\u6210\u53cc\u87ba\u65cb3D\u8d85\u7ed3\u6784\u3002<\/p>\n

\u56fe2. <\/b><\/strong>H47<\/b><\/sup><\/strong>RhuA-C(2H)\u56db\u805a\u4f53\u7684\u8bbe\u8ba1\u53ca\u86cb\u767d\u81ea\u7ec4\u88c5\u62103D\u7eb3\u7c73\u5e26<\/b><\/strong>\"\"<\/p>\n

(A)D47<\/sup>RhuA\u56db\u805a\u4f53\u5361\u901a\u56fe\u3002<\/p>\n

(B)H47<\/sup>RhuA-C(2H)\u00a0\u56db\u805a\u4f53\u5361\u901a\u56fe\u3002<\/p>\n

(C)3D\u7eb3\u7c73\u5e26\u7ec4\u88c5\u8fc7\u7a0b\u5361\u901a\u56fe\u3002\u5176\u4e2d(C1-C3) \u90bb\u8fd1\u7684H47<\/sup>RhuA-C(2H)\u00a0\u56db\u805a\u4f53\u95f4H47\u3001C(2H)\u95f4\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u5bfc\u81f4\u4e863D\u7eb3\u7c73\u5e26\u7684\u5f62\u6210\u3002<\/p>\n

(D)\u00a0\u8367\u5149\u63a2\u9488\u786b\u9ec4\u7d20-T\u8d1f\u67d3\u76843D\u7eb3\u7c73\u5e26\u7684\u8367\u5149\u56fe\u3002<\/p>\n

(E\uff0cF)\u00a03D\u7eb3\u7c73\u5e26\u7684\u4f4e\u500d\u548c\u9ad8\u500d\u7684TEM\u56fe\u50cf\u3002<\/p>\n

(G)\u00a03D\u7eb3\u7c73\u5e26\u76f4\u5f84\u7edf\u8ba1\u3002\u6570\u636e\u663e\u793a\u4e3a\u5e73\u5747\u503c\u00b1SD\u00a0(n = 50)\u3002<\/p>\n

(H)\u00a03D\u7eb3\u7c73\u5e26\u7684AFM\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u6cbf\u767d\u8272\u7bad\u5934\u7684\u8f6e\u5ed3\u3002<\/p>\n

(I)\u00a03D\u7eb3\u7c73\u5e26\u7684\u7ec4\u88c5\u7ed3\u6784\u793a\u610f\u56fe\u3002<\/p>\n

(J)\u00a03D\u7eb3\u7c73\u5e26\u7684SAXS\u7ed3\u679c\u3002<\/p>\n

\u56fe3 <\/b><\/strong>H47<\/b><\/sup><\/strong>RhuA-C(2H)\u86cb\u767d\u901a\u8fc7\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u81ea\u7ec4\u88c5\u62103D\u6676\u4f53<\/b><\/strong>\"\"<\/p>\n

(A)3D\u6676\u4f53\u7684\u8367\u5149\u7167\u7247\u3002<\/p>\n

(A1)\u00a03D\u6676\u4f53\u7684\u4f4e\u500d\u89c6\u56fe\u548c (A2)\u00a0\u9ad8\u500d\u89c6\u56fe\u3002<\/p>\n

(A3) 3D\u6676\u4f53\u7684\u9ad8\u5206\u8fa8\u7387TEM\u56fe\u50cf\uff1b\u6bd4\u4f8b\u5c3a\uff1a5 nm\u3002<\/p>\n

(A4) 3D\u6676\u4f53\u7684\u5085\u7acb\u53f6\u6570\u636e\u3002<\/p>\n

(B)\u00a03D\u6676\u4f53\u7684AFM\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u6cbf\u767d\u8272\u7bad\u5934\u7684\u8f6e\u5ed3\u3002<\/p>\n

(C)\u00a0\u7ec4\u88c5\u548c\u518d\u7ec4\u88c5\u76843D\u6676\u4f53\u7684SAXS\u7ed3\u679c\u3002<\/p>\n

(D)\u00a0H47<\/sup>RhuA-C(2H)\u00a0\u86cb\u767d\u81ea\u7ec4\u88c5\u62103D\u6676\u4f53\u7684\u793a\u610f\u56fe\u3002<\/p>\n

(E)\u00a0pH 2.8\u5904\u7406\u76843D\u6676\u4f53\u7684\u8367\u5149\u56fe\u548c (E1-E2)\u00a0TEM\u56fe\u50cf\u3002<\/p>\n

(F)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u8367\u5149\u56fe\u3002<\/p>\n

(F1)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u4f4e\u500d\u89c6\u56fe\u548c (F2)\u00a0\u9ad8\u500d\u89c6\u56fe\u3002<\/p>\n

(F3)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u9ad8\u5206\u8fa8\u7387TEM\u56fe\u50cf\uff1b\u6bd4\u4f8b\u5c3a\uff1a5 nm\u3002<\/p>\n

(F4)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u5085\u7acb\u53f6\u6570\u636e\u3002<\/p>\n

(G)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684AFM\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u6cbf\u767d\u8272\u7bad\u5934\u7684\u8f6e\u5ed3\u3002<\/p>\n

\u56fe4 <\/b><\/strong>Zn<\/b><\/strong>2+<\/b><\/sup><\/strong>\u8bf1\u5bfc<\/b><\/strong>H47<\/b><\/sup><\/strong>RhuA-C(2H)\u81ea\u7ec4\u88c5\u62103D\u6676\u4f53<\/b><\/strong>\"\"<\/p>\n

(A)\u00a0Zn2+<\/sup>\u8bf1\u5bfc\u76843D H47<\/sup>RhuA-C(2H) \u6676\u4f53\u7ed3\u6784\u793a\u610f\u56fe\u3002<\/p>\n

(B)\u00a0Zn2+<\/sup>\u8bf1\u5bfc\u76843D H47<\/sup>RhuA-C(2H) \u6676\u4f53\u7684\u8367\u5149\u7167\u7247\u3002<\/p>\n

(B1, B2) Zn2+<\/sup>\u8bf1\u5bfc\u76843D H47<\/sup>RhuA-C(2H) \u6676\u4f53\u7684\u4f4e\u3001\u9ad8\u500dTEM\u56fe\u50cf\u3002\u00a0<\/p>\n

(C)\u00a0Zn2+<\/sup>\u8bf1\u5bfc\u76843D H47<\/sup>RhuA-C(2H) \u6676\u4f53\u7684AFM\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u6cbf\u767d\u8272\u7bad\u5934\u7684\u8f6e\u5ed3\u3002<\/p>\n

(D)\u00a0Zn2+<\/sup>\u8bf1\u5bfc\u7684\u7ec4\u88c5\u548c\u518d\u7ec4\u88c53D H47<\/sup>RhuA-C(2H)\u6676\u4f53\u7684SAXS\u7ed3\u679c\u3002<\/p>\n

(E)\u00a010\u00a0mM EDTA\u5904\u7406\u540e\u76843D\u6676\u4f53\u7684\u8367\u5149\u56fe\u548c\u00a0(E1, E2) TEM\u56fe\u50cf\u3002<\/p>\n

(F)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684AFM\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u6cbf\u767d\u8272\u7bad\u5934\u7684\u8f6e\u5ed3\u3002<\/p>\n

(G)\u00a0\u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u8367\u5149\u56fe\u3002<\/p>\n

(G1, G2) \u518d\u7ec4\u88c53D\u6676\u4f53\u7684\u4f4e\u3001\u9ad8\u500dTEM\u56fe\u50cf\u3002<\/p>\n

\u56fe<\/b><\/strong>5<\/b><\/strong>\u00a0<\/b><\/strong>H47\/H157<\/b><\/sup><\/strong>RhuA-C(2H)-N(2H)\u56db\u805a\u4f53\u7684\u8bbe\u8ba1\u53ca\u86cb\u767d\u7684\u81ea\u7ec4\u88c5\u6210\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784<\/b><\/strong>\"\"<\/p>\n

(A)D47<\/sup>RhuA\u56db\u805a\u4f53\u5361\u901a\u56fe\u3002<\/p>\n

(B) H47\/H157<\/sup>RhuA-C(2H)-N(2H)\u00a0\u56db\u805a\u4f53\u5361\u901a\u56fe\u3002<\/p>\n

(C) H47\/H157<\/sup>RhuA-C(2H)-N(2H)\u00a0\u7ec4\u88c5\u6210\u5355\u4e2a\u7eb3\u7c73\u7ebf\u7684\u5361\u901a\u56fe\u3002<\/p>\n

(D-F)\u00a0\u90bb\u8fd1\u7684H47\/H157<\/sup>RhuA-C(2H)-N(2H)\u00a0\u56db\u805a\u4f53\u95f4H47\/C(2H)\u548cH157\/N(2H)\u7684\u975e\u5171\u4ef7\u76f8\u4e92\u4f5c\u7528\u5bfc\u81f4\u4e86\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784\u7684\u5f62\u6210\u3002<\/p>\n

(G)\u00a0\u5de6\u624b\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784\u7684\u793a\u610f\u56fe\u3002<\/p>\n

(H-K) \u5de6\u624b\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784\u7684\u4f4e\u3001\u9ad8\u500dTEM\u56fe\u50cf\u3002<\/p>\n

(L)\u00a0\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784\u87ba\u8ddd\u7edf\u8ba1\u7ed3\u679c\u3002\u6570\u636e\u663e\u793a\u4e3a\u5e73\u5747\u503c\u00b1SD (n = 50)\u3002<\/p>\n

\u3010\u5c0f\u7ed3<\/b><\/strong>\u3011<\/p>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u8be5\u56e2\u961f\u62a5\u9053\u4e86\u901a\u8fc7\u5229\u7528RhuA\u86cb\u767d\u7684\u6676\u4f53\u7ed3\u6784\uff0c\u7406\u6027\u8bbe\u8ba1\u548c\u6539\u9020\u5176\u8868\u9762\u7684\u529f\u80fd\u4f4d\u70b9\uff0c\u6210\u529f\u5b9e\u73b0\u4e86\u5404\u5411\u5f02\u6027\u86cb\u767d\u8d28\u57fa\u5143\u7684\u7cbe\u786e\u3001\u53ef\u63a7\u81ea\u7ec4\u88c5\uff1b\u5e76\u4e14\u6539\u53d8\u5916\u90e8\u6761\u4ef6\uff0c\u8c03\u63a7\u7ec4\u88c5\u8fc7\u7a0b\u7684\u4f5c\u7528\u529b\uff0c\u5b9e\u73b0\u4e86\u86cb\u767d\u8d28\u6676\u4f53\u7ed3\u6784\u5e94\u5bf9\u73af\u5883\u523a\u6fc0\u7684\u52a8\u6001\u54cd\u5e94\u3002\u5728\u8be5\u7814\u7a76\u4e2d\uff0c\u4e00\u4e2a\u5173\u952e\u7684\u8bbe\u8ba1\u56e0\u7d20\u662f\u5f15\u5165\u7ec4\u6c28\u9178\u7684\u03c0-\u03c0\u4f5c\u7528\uff0c\u8d4b\u4e88\u4e86\u7ec4\u88c5\u6676\u4f53\u7ed3\u6784\u7684\u7075\u6d3b\u6027\u548c\u52a8\u6001\u6027\uff0c\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u9009\u62e9\u6027\u8c03\u63a7\u5176\u4ed6\u7c7b\u578b\u7684\u76f8\u4e92\u4f5c\u7528\u529b\uff0c\u8fdb\u800c\u5b9e\u73b0\u4e86\u66f4\u52a0\u4e30\u5bcc\u7684\u81ea\u7ec4\u88c5\u7ed3\u6784\uff0c\u5305\u62ec3D\u7eb3\u7c73\u5e26\u3001\u4e24\u79cd\u7c7b\u578b\u76843D\u6676\u4f53\u548c\u53cc\u87ba\u65cb\u8d85\u7ed3\u6784\u3002\u6b63\u5982\u6211\u4eec\u7814\u7a76\u6240\u5c55\u793a\u7684\uff0c\u901a\u8fc7\u6df1\u5165\u7406\u89e3\u86cb\u767d\u8d28\u4e09\u7ef4\u7ed3\u6784\u548c\u7406\u6027\u8bbe\u8ba1\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u529b\uff0c\u5c06\u53ef\u4ee5\u5b9e\u73b0\u83b7\u5f97\u9ad8\u5ea6\u6709\u5e8f\u7684\u86cb\u767d\u8d28\u6676\u4f53\u4ee5\u53ca\u5bf9\u5176\u7ec4\u88c5\u8fc7\u7a0b\u8fdb\u884c\u52a8\u6001\u8c03\u63a7\u3002\u8fd9\u79cd\u63a7\u5236\u548c\u8c03\u63a7RhuA\u6676\u4f53\u7684\u7ed3\u6784\u6027\u8d28\u548c\u52a8\u6001\u6027\u7684\u72ec\u7279\u80fd\u529b\u5c06\u4e3a\u81ea\u9002\u5e94\u5206\u5b50\u6750\u6599\u7684\u5236\u9020\u63d0\u4f9b\u91cd\u8981\u542f\u793a\u3002<\/p>\n

\u3010\u56e2\u961f\u4ecb\u7ecd<\/b><\/strong>\u3011<\/p>\n

\u738b\u5f3a\u658c\u8bfe\u9898\u7ec4\u81ea2008\u5e74\u6210\u7acb\u4ee5\u6765\uff0c\u4e3b\u8981\u805a\u7126\u65b0\u578b\u65e0\u673a\u534a\u5bfc\u4f53\u8fd1\u7ea2\u5916\u4e8c\u533a\u8367\u5149\u91cf\u5b50\u70b9\u53ca\u5176\u8f6c\u5316\u533b\u5b66\u7814\u7a76\u548c\u751f\u7269\u5927\u5206\u5b50\uff08DNA\u548c\u86cb\u767d\u8d28\uff09\u6307\u5bfc\u7684\u7eb3\u7c73\u7ed3\u6784\u7cbe\u786e\u81ea\u7ec4\u88c5\u7814\u7a76\uff0c\u5728\u8fd1\u7ea2\u5916\u4e8c\u533a\u8367\u5149\u91cf\u5b50\u70b9\u65b9\u9762\uff1a\u9996\u5148\u63d0\u51fa\u548c\u53d1\u5c55\u4e86\u4e00\u79cd\u65b0\u578b\u7684\u8fd1\u7ea2\u5916\u4e8c\u533a\u8367\u5149Ag2<\/sub>S\u91cf\u5b50\u70b9\u4f53\u7cfb\u53ca\u5176\u5408\u6210\u6280\u672f\uff0c\u5728\u6b64\u57fa\u7840\u4e0a\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u7a84\u5e26\u9699\u7684\u534a\u5bfc\u4f53\u91cf\u5b50\u70b9\uff0c\u5c24\u5176\u662f\u8fd1\u671f\u53d1\u5c55\u7684\u9633\u79bb\u5b50\u63ba\u6742\u6280\u672f\uff0c\u663e\u8457\u63d0\u9ad8\u94f6\u57fa\u7a84\u5e26\u9699\u534a\u5bfc\u4f53\u91cf\u5b50\u70b9\u7684\u91cf\u5b50\u6548\u7387\uff1b\u201c\u4ece\u65e0\u5230\u6709\u201d\u81ea\u4e3b\u5f00\u53d1\u4e86\u4e00\u7cfb\u5217\u8fd1\u7ea2\u5916\u4e8c\u533a\u6b63\u7f6e\/\u5012\u7f6e\u8367\u5149\u663e\u5fae\u955c\u3001\u8367\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u3001\u5c0f\u52a8\u7269\u6d3b\u4f53\u6210\u50cf\u8bbe\u5907\uff0c\u4e3a\u8fd1\u7ea2\u5916\u4e8c\u533a\u8367\u5149\u7814\u7a76\u63d0\u4f9b\u4e86\u5fae\u89c2\u3001\u4ecb\u89c2\u548c\u5b8f\u89c2\u7684\u8de8\u5c3a\u5ea6\u6210\u50cf\u6280\u672f\u5e73\u53f0\uff1b\u53d1\u5c55\u4e86\u57fa\u4e8e\u8fd1\u7ea2\u5916\u4e8c\u533a\u8367\u5149Ag2<\/sub>S\u91cf\u5b50\u70b9\u7684\u6d3b\u4f53\u5f71\u50cf\u6280\u672f\uff0c\u5b9e\u73b0\u4e86\u5728\u5c0f\u52a8\u7269\u6d3b\u4f53\u6c34\u5e73\u539f\u4f4d\u3001\u5b9e\u65f6\u3001\u9ad8\u7075\u654f\u5ea6\u548c\u9ad8\u4fe1\u566a\u6bd4\u7684\u7cbe\u51c6\u533b\u5b66\u7814\u7a76\u3002\u5728\u81ea\u7ec4\u88c5\u7814\u7a76\u65b9\u9762\uff1a\u5229\u7528DNA\u548c\u86cb\u767d\u8d28\u751f\u7269\u5206\u5b50\u7684\u53ef\u8bbe\u8ba1\u7279\u6027\uff0c\u4ece\u5316\u5b66\u7684\u89d2\u5ea6\u7cbe\u51c6\u6784\u7b51\u4e86\u4e00\u7cfb\u5217\u65b0\u578b\u7684\u81ea\u7ec4\u88c5\u7eb3\u7c73\u7ed3\u6784\uff0c\u5b9e\u73b0\u4e86\u5728\u76ee\u6807\u7eb3\u7c73\u7ed3\u6784\u4e2d\u53ef\u63a7\u8c03\u53d8\u6784\u7b51\u57fa\u5143\u7684\u4e09\u7ef4\u7a7a\u95f4\u6392\u5e03\uff0c\u5e76\u5bf9\u5176\u65b0\u9896\u7684\u7406\u5316\u6027\u8d28\u8fdb\u884c\u4e86\u6df1\u5165\u7814\u7a76\u3002\u738b\u5f3a\u658c\u7814\u7a76\u5458\u8bfe\u9898\u7ec4\u53d1\u8868\u8bba\u6587120\u4f59\u7bc7\u5305\u62ecJ. Am. Chem. Soc.<\/i><\/em>, Angew. Chem. Int. Ed.<\/i><\/em>,\u00a0Adv. Mater.<\/i><\/em>,\u00a0Nano Lett.<\/i><\/em>\u7b49\u56fd\u9645\u77e5\u540d\u671f\u520a\uff0c\u7533\u8bf7\u4e13\u522940\u4f59\u9879\uff0c\u56fd\u9645\u4e13\u52295\u9879\u3002\u4e3b\u8981\u7814\u7a76\u6210\u679c\u83b72017\u5e74\u5ea6\u6c5f\u82cf\u7701\u79d1\u5b66\u6280\u672f\u4e00\u7b49\u5956\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/b><\/strong>Noncovalent Self-Assembly of Protein Crystals with Tunable Structures<\/u><\/a>\uff08Nano Lett.,\u00a02021\uff0cDOI:10.1021\/acs.nanolett.0c04587\uff09<\/p>\n

\u3010\u56e2\u961f\u5728\u8be5\u9886\u57df\u5de5\u4f5c\u6c47\u603b<\/b><\/strong>\u3011<\/p>\n

    \n
  1. Mingming Du, Kun Zhou, Runze Yu, Yufeng Zhai, Gang Chen,Qiangbin Wang*. Noncovalent Self-Assembly of Protein Crystals with Tunable Structures.\u00a0Nano Lett., 2021, DOI: 10.1021\/acs.nanolett.0c04587.<\/li>\n
  2. Kun Zhou+<\/sup>, Yihao Zhou+<\/sup>, Hongchao Yang, Huile Jin, Yonggang Ke*, Qiangbin Wang*. Interfacially Bridging Covalent Network Yields Hyperstable and Ultralong Virus-based Fibers for Engineering Functional Materials. Chem. Int. Ed., 2020, 59, 18249-18255.<\/li>\n
  3. Jinyi Dong+<\/sup>, Meng Wang+<\/sup>, Yihao Zhou,Chao Zhou*,\u00a0Qiangbin Wang*. DNA-Based Adaptive Plasmonic Logic Gates.\u00a0 Chem. Int. Ed.,\u00a02020, 59, 15038-15042.<\/li>\n
  4. Kun Zhou, Yihao Zhou, Victor Pan,Qiangbin Wang*, Yonggang Ke*. Programming Dynamic Assembly of Viral Proteins with DNA Origami.\u00a0 Am. Chem. Soc., 2020, 142, 5929-5932.<\/li>\n
  5. Mingming Du, Kun Zhou*, Xiao Wang, Jianting Zhang, Yejun Zhang, Jinchen Dong, Longlong Wu, Zhi Qiao, Gang Chen*, Qiangbin Wang*. Precise Fabrication of De Novo Nanoparticle Lattices on Dynamic 2D Protein Crystalline Lattices.Nano Lett., 2020, 20, 1154-1160.<\/li>\n
  6. Jianting Zhang, Kun Zhou, Yejun Zhang, Mingming Du,Qiangbin Wang*. Precise Self-Assembly of Nanoparticles into Ordered Nanoarchitectures Directed by Tobacco Mosaic Virus Coat Protein.\u00a0 Mater.,\u00a02019, 31,\u00a01901485.<\/li>\n
  7. Kun Zhou, Yonggang Ke, Qiangbin Wang*. Selective in Situ Assembly of Viral Protein onto DNA Origami. Am. Chem. Soc.,\u00a02018, 140, 8074-8077.<\/li>\n
  8. Chenqi Shen, Xiang Lan, Chenggan Zhu, Wei Zhang, Leyu Wang*,\u00a0Qiangbin Wang*. Spiral Patterning of Au Nanoparticles on Au Nanorod Surface to Form Chiral AuNR@AuNP Helical Superstructures Templated by DNA Origami.\u00a0Adv. Mater.,\u00a02017,\u00a029, 1606533.<\/li>\n
  9. Chenqi Shen, Xiang Lan, Xuxing Lu, Travis A. Meyer, Weihai Ni, Yonggang Ke*,\u00a0Qiangbin Wang*. Site-Specific Surface Functionalization of Gold Nanorods Using DNA Origami Clamps. Am. Chem. Soc., 2016, 138, 1764-1767.<\/li>\n
  10. Xiang Lan, Xuxing Lu, Chenqi Shen, Yonggang Ke, Weihai Ni,\u00a0Qiangbin Wang*.Au Nanorod Helical Superstructures with Designed Chirality.\u00a0J. Am. Chem. Soc.,\u00a02015, 137, 457-462.<\/li>\n<\/ol>\n

    \u3010\u76f8\u5173\u4f18\u8d28\u6587\u732e\u63a8\u8350<\/b><\/strong>\u3011<\/p>\n

      \n
    1. Mingming Du, Kun Zhou*, Xiao Wang, Jianting Zhang, Yejun Zhang, Jinchen Dong, Longlong Wu, Zhi Qiao, Gang Chen*, Qiangbin Wang*. Precise Fabrication of De Novo Nanoparticle Lattices on Dynamic 2D Protein Crystalline Lattices.Nano Lett., 2020, 20, 1154-1160.<\/li>\n
    2. Jianting Zhang, Kun Zhou, Yejun Zhang, Mingming Du,Qiangbin Wang*. Precise Self-Assembly of Nanoparticles into Ordered Nanoarchitectures Directed by Tobacco Mosaic Virus Coat Protein.\u00a0 Mater.,\u00a02019, 31,\u00a01901485.<\/li>\n
    3. Kun Zhou, Yonggang Ke, Qiangbin Wang*. Selective in Situ Assembly of Viral Protein onto DNA Origami. Am. Chem. Soc.,\u00a02018, 140, 8074-8077.<\/li>\n
    4. Chenqi Shen, Xiang Lan, Chenggan Zhu, Wei Zhang, Leyu Wang*,\u00a0Qiangbin Wang*. Spiral Patterning of Au Nanoparticles on Au Nanorod Surface to Form Chiral AuNR@AuNP Helical Superstructures Templated by DNA Origami.\u00a0Adv. Mater.,\u00a02017,\u00a029, 1606533.<\/li>\n
    5. Chenqi Shen, Xiang Lan, Xuxing Lu, Travis A. Meyer, Weihai Ni, Yonggang Ke*,\u00a0Qiangbin Wang*. Site-Specific Surface Functionalization of Gold Nanorods Using DNA Origami Clamps. Am. Chem. Soc., 2016, 138, 1764-1767.<\/li>\n
    6. Xiang Lan, Xuxing Lu, Chenqi Shen, Yonggang Ke, Weihai Ni,\u00a0Qiangbin Wang*.Au Nanorod Helical Superstructures with Designed Chirality.\u00a0J. Am. Chem. Soc.,\u00a02015, 137, 457-462.<\/li>\n<\/ol>\n

      \u672c\u6587\u7531\u6728\u6587\u97ec<\/b><\/strong>\u7ffb\u8bd1\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002<\/p>\n

      \u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com<\/u><\/a>\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

      \u3010\u5f15\u8a00\u3011 \u64cd\u63a7\u7eb3\u7c73\u7ea7\u6784\u7b51\u57fa\u5143\u7ec4\u88c5\u6210\u9ad8\u5ea6\u6709\u5e8f\u7684\u6676\u4f53\u7ed3\u6784\u5df2\u7ecf\u5438\u5f15\u4e86\u5927\u91cf\u7684\u7814\u7a76\u5174\u8da3\u3002\u901a\u8fc7\u4f18\u5316\u65e0\u673a\u7eb3\u7c73\u9897\u7c92\u7684\u7c7b\u578b\u3001\u5c3a\u5bf8\u3001\u6676\u683c\u53c2\u6570\u548c\u6676\u4f53\u5bf9\u79f0\u6027\uff0c\u53ef\u4ee5\u5728\u70ed\u529b\u5b66\u6216\u52a8\u529b\u5b66\u4e0a\u8c03\u63a7…<\/p>\n","protected":false},"author":2109,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[2927,4583,4584],"class_list":["post-218339","post","type-post","status-publish","format-standard","hentry","category-jiedu","tag-2927","tag-4583","tag-4584"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/218339"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/2109"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=218339"}],"version-history":[{"count":2,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/218339\/revisions"}],"predecessor-version":[{"id":218477,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/218339\/revisions\/218477"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=218339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=218339"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=218339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}