{"id":234315,"date":"2022-03-16T06:15:04","date_gmt":"2022-03-15T22:15:04","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=234315"},"modified":"2022-03-16T10:49:17","modified_gmt":"2022-03-16T02:49:17","slug":"%e5%89%91%e6%a1%a5%e5%a4%a7%e5%ad%a6nature-chemistry%ef%bc%9a%e8%87%aa%e7%84%b6%e8%b5%8b%e4%ba%88%e7%9a%84%e7%81%b5%e6%84%9f-%e5%b0%86%e4%ba%8c%e6%b0%a7%e5%8c%96%e7%a2%b3%e8%bd%ac","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=234315","title":{"rendered":"\u5251\u6865\u5927\u5b66Nature Chemistry\uff1a\u81ea\u7136\u8d4b\u4e88\u7684\u7075\u611f\u2014\u2014\u5c06\u4e8c\u6c27\u5316\u78b3\u8f6c\u5316\u4e3a\u6e05\u6d01\u71c3\u6599\u7684\u65b0\u6982\u5ff5"},"content":{"rendered":"

\u4e00\u3001 \u3010\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u78b3\u4e2d\u6027\u7ecf\u6d4e\u7684\u53d1\u5c55\u8981\u6c42\u6211\u4eec\u7528\u53ef\u6301\u7eed\u71c3\u6599\u548c\u53ef\u518d\u751f\u80fd\u6e90\u4e2d\u7684\u5316\u5b66\u6210\u5206\u53d6\u4ee3\u5bf9\u5316\u77f3\u71c3\u6599\u7684\u4f9d\u8d56\u3002\u5229\u7528\u53ef\u518d\u751f\u7535\u529b\u4ee5\u7535\u5316\u5b66\u4e8c\u6c27\u5316\u78b3\u51cf\u6392(CO2<\/sub>R)\u5f62\u5f0f\u8fdb\u884c\u7684\u78b3\u6355\u83b7\u548c\u5229\u7528\u662f\u76ee\u524d\u5e94\u5bf9\u5168\u7403\u6c14\u5019\u9762\u6696\u7684\u4e00\u79cd\u53ef\u9884\u89c1\u7684\u529e\u6cd5\u3002\u4f46\u662f\uff0c\u5c31\u76ee\u524d\u6280\u672f\u6765\u770b\uff0c\u7535\u50ac\u5316CO2<\/sub>\u8fd8\u539f\u7684\u591a\u76f8\u50ac\u5316\u5242\uff0c\u5176\u6027\u80fd\u5728\u6240\u9700\u8f83\u5927\u8fc7\u7535\u4f4d\u4e0b\uff0c\u5bb9\u6613\u53d7\u5230\u526f\u53cd\u5e94\u548c\u8f83\u4f4e\u7684\u52a8\u529b\u5b66\u6548\u7387\u5f71\u54cd\u3002<\/p>\n

\u5728\u81ea\u7136\u754c\u4e2d\uff0c\u78b3\u9178\u9150\u9176(carbonic anhydrase, CA)\u7ecf\u5e38\u7528\u6765\u50ac\u5316CO2<\/sub>\u6c34\u5408\u4f5c\u7528\u3002CA\u662f\u4e00\u79cd\u975e\u6c27\u5316\u8fd8\u539f\u9176\uff0c\u5728\u8bb8\u591a\u4ee3\u8c22\u8fc7\u7a0b\u4e2d\u8d77\u7740\u5173\u952e\u4f5c\u7528\u3002CA\u8fd8\u80fd\u50ac\u5316HCO3<\/sub>–<\/sup>\u53cd\u5e94\u751f\u6210CO2<\/sub>\u3002\u9176\u4fc3CO2R\u7535\u50ac\u5316\u5242\uff0c\u5982\u7532\u9178\u8131\u6c22\u9176(formate dehydrogenase, FDh)\u5c55\u73b0\u51fa\u4e86\u7c7b\u4f3c\u4e8e\u975e\u5747\u76f8\u50ac\u5316\u5242\u7684\u5316\u5b66\u53cd\u5e94\u3002\u867d\u7136FDh\u5177\u6709\u9ad8\u6548\u7684\u9009\u62e9\u6027\uff0c\u4f46\u662f\u5b83\u5bf9\u73af\u5883pH\u9ad8\u5ea6\u654f\u611f\u3002<\/p>\n

\u4e8c\u3001\u3010\u6210\u679c\u63a0\u5f71\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u6765\u81ea\u82f1\u56fd\u5251\u6865\u5927\u5b66<\/strong>Erwin Reisner<\/strong>\u56e2\u961f\u5728<\/strong>Nature Chemistry<\/em><\/strong>\u4e0a<\/strong>\u53d1\u8868\u6587\u7ae0\uff0c\u9898\u4e3a\u201cFast CO2<\/sub> hydration kinetics impair heterogeneous but improve enzymatic CO2<\/sub> reduction catalysis\u201d\u3002 \u5251\u6865\u5927\u5b66\u5f00\u53d1\u7684\u8fd9\u4e00\u6982\u5ff5\u7684\u8bc1\u660e\u4f9d\u8d56\u4e8e\u4ece\u7ec6\u83cc\u4e2d\u5206\u79bb\u51fa\u6765\u7684\u9176\uff0c\u4e3a\u5c06\u4e8c\u6c27\u5316\u78b3\u8f6c\u5316\u4e3a\u71c3\u6599\u7684\u5316\u5b66\u53cd\u5e94\u63d0\u4f9b\u52a8\u529b\uff0c\u8fd9\u4e00\u8fc7\u7a0b\u88ab\u79f0\u4e3a\u7535\u89e3\u3002\u9176\u6bd4\u5176\u4ed6\u50ac\u5316\u5242(\u5982\u91d1)\u66f4\u6709\u6548\uff0c\u4f46\u5b83\u4eec\u5bf9\u5316\u5b66\u73af\u5883\u9ad8\u5ea6\u654f\u611f\u3002\u5982\u679c\u6240\u5904\u73af\u5883\u4e0d\u5408\u9002\u9176\u7684\u5b58\u6d3b\uff0c\u9176\u5c31\u4f1a\u5206\u89e3\uff0c\u5316\u5b66\u53cd\u5e94\u5c31\u4f1a\u53d8\u6162\u3002\u5251\u6865\u5927\u5b66\u7684\u7814\u7a76\u4eba\u5458\u4e0e\u8461\u8404\u7259\u65b0\u91cc\u65af\u672c\u5927\u5b66\u7684\u4e00\u4e2a\u56e2\u961f\u5408\u4f5c\uff0c\u5df2\u7ecf\u5f00\u53d1\u51fa\u4e00\u79cd\u65b9\u6cd5\uff0c\u901a\u8fc7\u5fae\u8c03\u6eb6\u6db2\u6761\u4ef6\u6765\u6539\u53d8\u9176\u6240\u5904\u7684\u73af\u5883\uff0c\u4ece\u800c\u63d0\u9ad8\u7535\u89e3\u6548\u7387\u3002<\/p>\n

\u9176\u5df2\u7ecf\u8fdb\u5316\u4e86\u6570\u767e\u4e07\u5e74\uff0c\u53d8\u5f97\u975e\u5e38\u9ad8\u6548\u548c\u6709\u9009\u62e9\u6027\uff0c\u56e0\u4e3a\u6ca1\u6709\u4efb\u4f55\u4e0d\u60f3\u8981\u7684\u526f\u4ea7\u54c1\uff0c\u6240\u4ee5\u5b83\u4eec\u975e\u5e38\u9002\u5408\u71c3\u6599\u751f\u4ea7\u3002\u7136\u800c\uff0c\u9176\u7684\u654f\u611f\u6027\u5e26\u6765\u4e86\u4e00\u7cfb\u5217\u4e0d\u540c\u7684\u6311\u6218\u3002\u56e0\u6b64\u5728\u8bbe\u8ba1\u50ac\u5316\u5242\u7684\u540c\u65f6\u9700\u8981\u8003\u8651\u8fd9\u79cd\u654f\u611f\u6027\uff0c\u4ee5\u4fbf\u8c03\u6574\u6eb6\u6db2\u73af\u5883\u4ee5\u9002\u5e94\u9176\u7684\u7406\u60f3\u5de5\u4f5c\u6761\u4ef6\u3002\u7814\u7a76\u4eba\u5458\u6700\u65b0\u7814\u7a76\u5728\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e0b\u5c06\u71c3\u6599\u751f\u4ea7\u6548\u7387\u63d0\u9ad8\u4e8618\u500d\uff0c\u8bc1\u660e\u4e86\u6c61\u67d3\u7684\u78b3\u6392\u653e\u53ef\u4ee5\u6709\u6548\u5730\u8f6c\u5316\u4e3a\u7eff\u8272\u71c3\u6599\uff0c\u800c\u4e0d\u4f1a\u6d6a\u8d39\u4efb\u4f55\u80fd\u6e90\u3002\u4e3a\u4e86\u8fdb\u4e00\u6b65\u6539\u5584\u6eb6\u6db2\u73af\u5883\uff0c\u7814\u7a76\u5c0f\u7ec4\u5c55\u793a\u4e86\u4e24\u79cd\u9176\u5982\u4f55\u534f\u540c\u5de5\u4f5c\uff0c\u4e00\u79cd\u4ea7\u751f\u71c3\u6599\uff0c\u53e6\u4e00\u79cd\u63a7\u5236\u73af\u5883\u3002\u4ed6\u4eec\u53d1\u73b0\uff0c\u901a\u8fc7\u6dfb\u52a0\u53e6\u4e00\u79cd\u9176\uff0c\u53cd\u5e94\u901f\u5ea6\u52a0\u5feb\uff0c\u65e2\u63d0\u9ad8\u4e86\u6548\u7387\uff0c\u53c8\u51cf\u5c11\u4e86\u4e0d\u5fc5\u8981\u7684\u526f\u4ea7\u7269\u3002<\/p>\n

\u672c\u6587\u7684\u901a\u8baf\u4f5c\u8005Erwin Reisner\u8bf4\u9053\uff1a\u201c\u7535\u89e3\u5728\u51cf\u5c11\u78b3\u6392\u653e\u65b9\u9762\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u6211\u4eec\u5c55\u793a\u4e86\u4e00\u79cd\u65b0\u7684\u6982\u5ff5\uff0c\u5373\u6355\u6349\u78b3\u5e76\u4ee5\u8282\u80fd\u7684\u65b9\u5f0f\u5c06\u5176\u8f6c\u5316\u4e3a\u6709\u7528\u7684\u4e1c\u897f\uff0c\u800c\u4e0d\u662f\u6355\u6349\u548c\u50a8\u5b58\u4e8c\u6c27\u5316\u78b3\uff0c\u8fd9\u662f\u4e00\u9879\u96be\u4ee5\u7f6e\u4fe1\u7684\u80fd\u6e90\u5bc6\u96c6\u578b\u5de5\u4f5c<\/em><\/span>\u201d\u3002<\/p>\n

\"\"<\/p>\n

\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/strong><\/p>\n

\u221a\u901a\u8fc7\u78b3\u9178\u9150\u9176\u7684\u5171\u56fa\u5b9a\u5316<\/strong><\/span>\uff0c\u7814\u7a76\u4e86CO2<\/sub>\u6c34\u5408\u5bf9\u5c40\u90e8\u73af\u5883\u5f71\u54cd<\/span><\/strong>\uff0c\u4ee5\u53ca\u4ece\u7532\u9178\u8131\u6c22\u9176\u5230\u5f02\u8d28\u7cfb\u7edf\u7684\u4e00\u7cfb\u5217\u4e0d\u540cCO2<\/sub>\u8fd8\u539f\u7cfb\u7edf\u6027\u80fd\uff1b<\/p>\n

\u221a \u78b3\u9178\u9150\u9176\u5171\u56fa\u5b9a\u5316\uff0c\u589e\u52a0\u4e86\u7535\u6781\u4e0aCO2<\/sub>\u6c34\u5408\u52a8\u529b\u5b66<\/span><\/strong>\u3002\u5c3d\u7ba1CO2<\/sub>\u6d53\u5ea6\u964d\u4f4e\uff0c\u4f46\u7531\u4e8e\u5c40\u90e8pH\u53d8\u5316\u51cf\u5c11\uff0c\u8fd9\u6709\u5229\u4e8e\u9176\u4fc3CO2<\/sub>\u8fd8\u539f\u3002<\/p>\n

\u56db\u3001\u3010\u6570\u636e\u6982\u89c8\u3011<\/strong><\/p>\n

\u56fe1<\/strong> \u4ecb\u5b54\u6c27\u5316\u94df\u9521(indium tin oxide ITO)\u7535\u6781\u9176\u7684\u5171\u56fa\u5b9a\u5316 \u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"a. \u4ecb\u5b54ITO\u7535\u6781\u56fe\u7247<\/p>\n

b. \u4ecb\u5b54ITO\u7535\u6781\u7684\u626b\u63cf\u7535\u955c\u622a\u9762\u56fe<\/p>\n

c. ITO\u591a\u5b54\u7535\u6781\u56fa\u5b9a\u5316\u9176\u53ca\u5176\u50ac\u5316\u53cd\u5e94\u7684\u539f\u7406\u63cf\u8ff0<\/p>\n

\u56fe2<\/strong> \u00a0[NiFeSe]-\u6c22\u5316\u9176(H2<\/sub>ase) \u6216FDh\u4e0eCA\u5728\u5e73\u9762\u6c27\u5316\u94df\u9521ITO\u4e0a\u7684\u5171\u56fa\u5b9a\u00a0\u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"<\/p>\n

a. H2ase\u548cCA\u5171\u56fa\u5b9a\u5316<\/p>\n

b. FDh\u548cCA\u5171\u56fa\u5b9a\u5316<\/p>\n

\u56fe3<\/strong> \u5171\u56fa\u5b9a\u548c\u672a\u5171\u56fa\u5b9aH2ase\u7684\u7535\u5316\u5b66\u6027\u80fd\u548c\u6a21\u62df\u5c40\u90e8\u73af\u5883\u00a0\u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"a. \u5728\u4ecb\u5b54ITO\u7535\u6781\u4e0a\u56fa\u5b9a20 pmol H2ase\u7684\u53ef\u63a7\u7535\u4f4d\u7535\u89e3\u5236\u6c22\u7684\u5c40\u90e8\u7535\u6d41\u5bc6\u5ea6\u5b9e\u9a8c\u548c\u6a21\u62df\u66f2\u7ebf<\/p>\n

b. \u5728\u4e0d\u540c\u7535\u4f4d\u4e0b\uff0c\u6709\u9650\u5143\u8ba1\u7b97\u5f97\u5230\u7684\u7a33\u6001\u591a\u5b54\u7535\u6781\u5185\u7684\u5e73\u5747pH\u503c<\/p>\n

c. pH\u503c\u5bf9\u6d3b\u6027\u7684\u5f71\u54cd<\/p>\n

\u56fe4<\/strong> \u00a0\u6709\u548c\u6ca1\u6709\u9499\u5171\u56fa\u5b9a\u7684\u7532\u9178\u8131\u6c22\u9176\u7535\u5316\u5b66\u6027\u80fd\u548c\u6a21\u62df\u5c40\u90e8\u73af\u5883\u00a0\u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"a. HCOO–<\/sup>\u79bb\u5b50\u8272\u8c31\u5b9a\u91cf\u7684\u5c40\u90e8\u7535\u6d41\u5bc6\u5ea6\u7684\u5b9e\u9a8c\u548c\u6a21\u62df\u66f2\u7ebf<\/p>\n

b, c. \u7528\u6709\u9650\u5143\u6cd5\u8ba1\u7b97\u7a33\u6001\u4e0b\u4ecb\u5b54\u7535\u6781\u5185\u7684\u5e73\u5747pH\u503c(b)\u548cCO2<\/sub>\u6d53\u5ea6(c)<\/p>\n

d. CO2<\/sub>\u6d53\u5ea6\u5bf9CO2R\u6d3b\u6027\u7684\u5f71\u54cd<\/p>\n

e. pH\u503c\u5bf9\u6d3b\u6027\u7684\u5f71\u54cd<\/p>\n

\u56fe5<\/strong> \u00a0\u57280.1 M KHCO3<\/sub>\u4e2d\u5171\u56fa\u5b9a\u548c\u672a\u5171\u56fa\u5b9aCa\u7684\u60c5\u51b5\u4e0b\uff0cFDh\u4e0eH2ase\u5171\u56fa\u5b9a\u7684\u7535\u5316\u5b66\u6027\u80fd\u00a0\u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"<\/p>\n

a. \u901a\u8fc7\u63a7\u5236\u7535\u4f4d\u7535\u89e320 pmol H2ase\u548c50 pmol FDh\u5171\u56fa\u5b9a\u5728\u4ecb\u5b54ITO\u7535\u6781\u4e0a\u7684\u603b\u7535\u6d41\u5bc6\u5ea6(\u7d2b\u8272)\u548cH2<\/sub>(\u6a59\u8272)\u548cHCOO\u2212<\/sup>(\u84dd\u8272)\u7684\u5c40\u90e8\u7535\u6d41\u5bc6\u5ea6\u7684\u5b9e\u9a8c(\u70b9)\u548c\u6a21\u62df(\u7ebf)\u66f2\u7ebf<\/p>\n

b. H2<\/sub>(\u6a59\u8272)\uff0cHCOO\u2212<\/sup>(\u84dd\u8272)\u548c\u603b(\u7d2b\u8272)FE\u7684\u5b9e\u9a8c(\u70b9)\u548c\u6a21\u62df(\u7ebf)\u66f2\u7ebf<\/p>\n

c, d. \u5e73\u5747pH (c)\u548cpH\u5bf9\u6d3b\u6027\u7684\u5f71\u54cd(d)<\/p>\n

\u56fe6<\/strong> \u57280.1 M KHCO3<\/sub>\u6eb6\u6db2\u4e2d\u6709\u548c\u6ca1\u6709Ca\uff0820 \u03bcM\uff09\u7684Au\u4e0a\u7684CO2R\u00a0\u00a9 2022 Springer Nature<\/span><\/p>\n

\"\"<\/p>\n

a. H2<\/sub>(\u6a59\u8272)\u548cCO(\u84dd\u8272)\u5728Au\u4e0a\u7528\u53ef\u63a7\u7535\u4f4d\u7535\u89e3\u65f6\u7684\u603b\u7535\u6d41\u5bc6\u5ea6(\u7d2b\u8272)\u548c\u5c40\u90e8\u7535\u6d41\u5bc6\u5ea6(\u6a59\u8272)<\/p>\n

b. H2<\/sub>(\u6a59\u8272)\uff0cCO(\u84dd\u8272)\u548c\u603bFE(\u7d2b\u8272)\u7684\u5b9e\u9a8c(\u70b9)\u548c\u6a21\u62df(\u7ebf)\u66f2\u7ebf<\/p>\n

c. \u7535\u6781\u8868\u9762CO2<\/sub>(\u84dd\u8272)\u3001HCO3<\/sub>–<\/sup>(\u6a59\u8272)\u548cCO3<\/sub>2\u2212<\/sup>(\u7d2b\u8272)\u7684\u6d53\u5ea6\u6a21\u62df<\/p>\n

d. \u7528\u6709\u9650\u5143\u6cd5\u8ba1\u7b97\u7535\u89e3\u6db2\u4e2d\u6709(\u5b9e\u7ebf)\u548c\u6ca1\u6709(\u865a\u7ebf)CA\u65f6\u7535\u6781\u8868\u9762\u7684pH\u503c<\/p>\n

\u4e94\u3001\u3010\u6210\u679c\u542f\u793a\u3011<\/strong><\/p>\n

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\u6587\u732e\u94fe\u63a5\uff1aFast CO2<\/sub> hydration kinetics impair heterogeneous but improve enzymatic CO2 reduction catalysis<\/a>. 2022, Nature Chemistry<\/em>, DOI: 10.1038\/s41557-021-00880-2<\/p>\n

\u672c\u6587\u7531\u7eb3\u7c73\u5c0f\u767d\u4f9b\u7a3f<\/p>\n

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