{"id":245365,"date":"2023-01-03T09:45:12","date_gmt":"2023-01-03T01:45:12","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=245365"},"modified":"2023-01-03T09:36:40","modified_gmt":"2023-01-03T01:36:40","slug":"%e5%8c%97%e4%ba%ac%e5%a4%a7%e5%ad%a6chem-sci-%ef%bc%9a%e7%94%a8%e4%ba%8e%e7%94%9f%e7%89%a9%e5%81%b6%e8%81%94%e7%9a%84%e7%a1%ab%e9%86%87-%e6%b0%a7%e5%8c%96%e7%a1%ab%e5%8f%b6%e7%ab%8b%e5%be%b7%e5%85%89","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=245365","title":{"rendered":"\u5317\u4eac\u5927\u5b66Chem. Sci.\uff1a\u7528\u4e8e\u751f\u7269\u5076\u8054\u7684\u786b\u9187-\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5149\u70b9\u51fb\u53cd\u5e94"},"content":{"rendered":"<p><strong>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p>\u86cb\u767d\u8d28\u5316\u5b66\u4fee\u9970\u5de5\u5177\u7684\u5f00\u53d1\u4fc3\u8fdb\u4e86\u5316\u5b66\u751f\u7269\u5b66\u548c\u836f\u5b66\u7814\u7a76\u7684\u8fdb\u6b65\u3002\u5176\u4e2d\uff0c\u86cb\u767d\u8d28\u534a\u80f1\u6c28\u9178\uff08Cys\uff09\u6b8b\u57fa\u7684\u9009\u62e9\u6027\u4fee\u9970\u53d1\u6325\u7740\u6781\u4e3a\u91cd\u8981\u7684\u4f5c\u7528\u3002\u8fd1\u5e74\u6765\uff0c\u7814\u7a76\u8005\u5bf9\u53ef\u89c1\u5149\u4ecb\u5bfc\u7684\u534a\u80f1\u6c28\u9178\u9009\u62e9\u6027\u751f\u7269\u5076\u8054\u8fdb\u884c\u4e86\u5927\u91cf\u7814\u7a76\u3002\u4f46\u662f\uff0c\u4f20\u7edf\u7684\u86cb\u767d\u8d28Cys\u6b8b\u57fa\u4fee\u9970\u53cd\u5e94\u7531\u4e8e\u5176\u4ed6\u4eb2\u6838\u6027\u6b8b\u57fa\u7684\u5b58\u5728\uff0c\u9009\u62e9\u6027\u901a\u5e38\u4e0d\u9ad8\u3002\u800c\u8fd1\u5e74\u6765\u5f00\u53d1\u7684\u53ef\u89c1\u5149\u4fc3\u8fdb\u7684Cys\u6b8b\u57fa\u4fee\u9970\u53cd\u5e94\u53c8\u5b58\u5728\u53cd\u5e94\u6761\u4ef6\u82db\u523b\u3001\u751f\u7269\u76f8\u5bb9\u6027\u4f4e\u548c\u53cd\u5e94\u901f\u7387\u8f83\u6162\u7b49\u95ee\u9898\u3002<\/p>\n<p><strong>\u4e8c\u3001\u3010\u6210\u679c\u63a0\u5f71\u3011<\/strong><\/p>\n<p>\u4e3a\u4e86\u89e3\u51b3\u4ee5\u4e0a\u96be\u9898\uff0c<strong>\u5317\u4eac\u5927\u5b66\u6df1\u5733\u7814\u7a76\u751f\u9662\u674e\u5b50\u521a\u6559\u6388\u3001\u6df1\u5733\u6e7e\u5b9e\u9a8c\u5ba4\u576a\u5c71\u751f\u7269\u533b\u836f\u7814\u53d1\u8f6c\u5316\u4e2d\u5fc3\u5c39\u4e30\u7814\u7a76\u5458\u3001\u738b\u854a\u7814\u7a76\u5458<\/strong>\u8054\u5408\u62a5\u9053\u4e86\u4e00\u79cd\u65e0\u91d1\u5c5e\u7684\u786b\u9187-\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5149\u70b9\u51fb\u53cd\u5e94\uff0c\u8be5\u53cd\u5e94\u80fd\u591f\u5728\u751f\u7269\u76f8\u5bb9\u6761\u4ef6\u4e0b\u5b9e\u73b0\u9ad8\u6548\u548c\u9ad8\u9009\u62e9\u6027\u7684\u751f\u7269\u5076\u8054\u53cd\u5e94\u3002\u901a\u8fc7\u673a\u7406\u7814\u7a76\uff0c\u4f5c\u8005\u63d0\u51fa\u4e86\u4e00\u4e2a\u5149\u50ac\u5316\u6d3b\u5316\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5f97\u5230\u9ad8\u6d3b\u6027\u6c27\u5316\u950d\u76d0\u4e2d\u95f4\u4f53\u7684\u673a\u7406\u3002\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5316\u5408\u7269\u672c\u8eab\u7684\u6c34\u6eb6\u6027\u6781\u597d\uff0c\u800c\u4e14\u8be5\u53cd\u5e94\u4f7f\u7528\u7684\u5149\u50ac\u5316\u5242\uff08\u6838\u9ec4\u7d20\u884d\u751f\u7269\uff09\u662f\u4e00\u7c7b\u5e38\u89c1\u7684\u751f\u7269\u6d3b\u6027\u7269\u8d28\uff0c\u56e0\u6b64\u63d0\u793a\u4e86\u8be5\u53cd\u5e94\u6f5c\u5728\u7684\u4f18\u79c0\u751f\u7269\u76f8\u5bb9\u6027\u3002\u6700\u540e\uff0c\u901a\u8fc7\u751f\u7269\u7d20\u884d\u751f\u7684\u6c27\u5316\u786b\u5e95\u7269\uff0c\u5bf9\u7ec6\u80de\u88c2\u89e3\u6db2\u8fdb\u884c\u4e86\u86cb\u767d\u7ec4\u5b66\u7814\u7a76\uff0c\u53d1\u73b0\u4e86\u5176\u5bf9\u86cb\u767d\u8d28\u7ec4\u4e2dCys\u6b8b\u57fa\u7684\u9009\u62e9\u6027\u8d85\u8fc790%\uff0c\u518d\u6b21\u8868\u660e\u4e86\u8be5\u53cd\u5e94\u826f\u597d\u7684\u5316\u5b66\u9009\u62e9\u6027\u548c\u6f5c\u5728\u7684\u751f\u7269\u5b66\u5e94\u7528\u4ef7\u503c\u3002\u8be5\u8bba\u6587\u4ee5\u9898\u4e3a\u201c<strong>The thiol-sulfoxonium ylide photo-click reaction for bioconjugation\u201d<\/strong>\u53d1\u8868\u5728\u77e5\u540d\u671f\u520a<strong><em>Chem. Sci<\/em><\/strong>\u4e0a\uff0c\u5317\u4eac\u5927\u5b66\u6df1\u5733\u7814\u7a76\u751f\u9662\u4e07\u5ddd\u535a\u58eb\u3001\u7814\u7a76\u751f\u4faf\u5360\u5cf0\u548c\u4ef2\u607a\u519c\u4e1a\u5de5\u7a0b\u5b66\u9662\u6768\u51ac\u71d5\u535a\u58eb\u4e3a\u8be5\u8bba\u6587\u7684\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245366\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/0-15.png\" alt=\"\" width=\"600\" height=\"225\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/0-15.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/0-15-300x113.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/strong><\/p>\n<p>\u901a\u8fc7\u4e00\u7cfb\u5217\u6761\u4ef6\u7b5b\u9009\uff0c\u4f18\u5316\u5f97\u5230\u4e86\u4e00\u4e2a\u9ad8\u6548\u548c\u9ad8\u9009\u62e9\u6027\u7684\u5def\u57fa-\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5149\u70b9\u51fb\u53cd\u5e94\uff0c\u5e76\u63d0\u51fa\u4e86\u4e00\u4e2a\u5149\u50ac\u5316\u6d3b\u5316\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5f97\u5230\u9ad8\u6d3b\u6027\u6c27\u5316\u950d\u76d0\u4e2d\u95f4\u4f53\u7684\u673a\u7406\uff0c\u8fdb\u4e00\u6b65\u901a\u8fc7\u5bf9\u542bCys\u6b8b\u57fa\u7684\u591a\u80bd\u548c\u86cb\u767d\u8d28\u7684\u5316\u5b66\u4fee\u9970\uff0c\u9a8c\u8bc1\u4e86\u8be5\u53cd\u5e94\u7684\u6781\u5feb\u7684\u53cd\u5e94\u6548\u7387\u548c\u5316\u5b66\u9009\u62e9\u6027\u3002<\/p>\n<p><strong>\u56db\u3001\u3010\u6570\u636e\u6982\u89c8\u3011<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245367\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/1-23.png\" alt=\"\" width=\"600\" height=\"487\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/1-23.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/1-23-300x244.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e00\u3001\u534a\u80f1\u6c28\u9178\u9009\u62e9\u6027\u751f\u7269\u5076\u8054\u53cd\u5e94<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\uff08a\uff09\u6587\u732e\u62a5\u9053\u534a\u80f1\u6c28\u9178\u9009\u62e9\u6027\u751f\u7269\u5076\u8054\u7684\u65b9\u6cd5\u3002<\/p>\n<p>\uff08b\uff09\u672c\u6587\u62a5\u9053\u7684\u53ef\u89c1\u5149\u8bf1\u5bfc\u7684\u5def\u57fa-\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u70b9\u51fb\u53cd\u5e94\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245368\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/2-23.png\" alt=\"\" width=\"600\" height=\"382\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/2-23.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/2-23-300x191.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e8c\u3001\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u4e0e\u5def\u57fa\u53cd\u5e94\u7684\u8303\u56f4<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\u5316\u5408\u7269<strong>1<\/strong>\uff081 mmol\uff0c2\u5f53\u91cf\uff09\uff0c\u786b\u9187<strong>2-6<\/strong>\uff080.5 mmol\uff0c1\u5f53\u91cf\uff09\uff0cRFTA\uff082% mol\uff09\u6df7\u5408\u540e\u5728\u5ba4\u6e29\u4e0b8 mL\u6c34\u4e2d\u7528\u53ef\u89c1\u5149\uff08450 nm\uff09\u4e0b\u7167\u5c0420\u5206\u949f\u53cd\u5e94\u7684\u4ea7\u7387\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245369\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/3-23.png\" alt=\"\" width=\"600\" height=\"309\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/3-23.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/3-23-300x155.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e09\u3001\u673a\u7406\u7814\u7a76<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\uff08a\uff09RFTA\u548c<strong>1a<\/strong>\u7684\u7d2b\u5916-\u53ef\u89c1\u5149\u5438\u6536\u5149\u8c31\u3002<\/p>\n<p>\uff08b\uff09\u5149\u50ac\u5316\u5242RFTA\u548c\u6c27\u5316\u786b\u53f6\u7acb\u5fb7<strong>1a<\/strong>\u4e4b\u95f4\u7684Stern-Volmer\u8367\u5149\u731d\u706d\u5b9e\u9a8c\u3002<\/p>\n<p>\uff08c\uff09\u5bf9\u7167\u5b9e\u9a8c\uff1a<strong>1a<\/strong>\u7684\u5149\u5206\u89e3\u3002<\/p>\n<p>\uff08d\uff09\u6c18\u4ee3\u6eb6\u5242\u4e2d\u7684\u6c22\u6e90\u3002<\/p>\n<p>\uff08e\uff09\u63a8\u6d4b\u7684\u53cd\u5e94\u673a\u7406\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245370\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/4-22.png\" alt=\"\" width=\"600\" height=\"518\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/4-22.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/4-22-300x259.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u56db\u3001<\/strong><strong>Cys<\/strong><strong>\u9009\u62e9\u6027\u80bd\u4fee\u9970\u7684\u8303\u56f4<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\u5728PBS\u7f13\u51b2\u6db2\uff08pH 7.4\uff0c\u52a0\u516525%MeCN\u4ee5\u6eb6\u89e3\u80bd\uff09\u4e2d\uff0c\u5ba4\u6e29\u53ef\u89c1\u5149\u7167\u5c04\u4e0b\uff0c\u5c06\u6c27\u5316\u786b\u53f6\u7acb\u5fb7<strong>1<\/strong>\uff0810 mM\uff09\u3001\u80bd<strong>7-14<\/strong>\uff081 mM\uff09\u3001RFTA\uff080.5 mM\uff09\u548c\u786b\u8132\uff0810 mM\uff09\u4f5c\u4e3a\u6eb6\u5242\uff0c\u6301\u7eed\u53cd\u5e941\u5206\u949f\uff0c\u901a\u8fc7LC-MS\u6d4b\u5b9a\u4ea7\u7387\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245371\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/5-15.png\" alt=\"\" width=\"600\" height=\"437\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/5-15.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/5-15-300x219.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e94\u3001\u86cb\u767d\u8d28\u7684\u534a\u80f1\u6c28\u9178\u7279\u5f02\u6027\u4fee\u9970<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\uff08a\uff09\u53cd\u5e94\u6761\u4ef6\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09<strong>BSA-1a<\/strong>\u52a0\u5408\u7269\u7684ESI-TOF MS\u5206\u6790\u3002<\/p>\n<p>\uff08c\uff09<strong>BSA-1m<\/strong>\u52a0\u5408\u7269\u7684ESI-TOF MS\u5206\u6790\u3002<\/p>\n<p>\uff08d\uff09\u6cdb\u7d20\u7ed3\u5408\u91762C-<strong>1a<\/strong>\u52a0\u5408\u7269\u7684ESI-TOF MS\u5206\u6790\u3002<\/p>\n<p>\uff08e\uff09Cys34\u4e0a<strong>BSA-1a<\/strong>\u7684MS\/MS\u5206\u6790\u3002<\/p>\n<p>\uff08f\uff09BSA\u52a0\u5408\u7269\u7684\u5706\u4e8c\u8272\u6027\uff08CD\uff09\u5206\u6790\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-245372\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/6-9.png\" alt=\"\" width=\"600\" height=\"562\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/6-9.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2022\/12\/6-9-300x281.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u516d\u3001\u786b\u9187<\/strong><strong>&#8211;<\/strong><strong>\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5149\u70b9\u51fb\u53cd\u5e94\u7684\u86cb\u767d\u8d28\u7ec4\u5b66\u5e94\u7528<\/strong><strong> \u00a9 2022 The Author<\/strong><\/p>\n<p>\uff08a\uff09\u57fa\u4e8e\u51dd\u80f6\u548cMS\u7684\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u5de5\u4f5c\u6d41\u7a0b\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09<strong>BSA-1m<\/strong>\u7684WB\u5206\u6790\u3002<\/p>\n<p>\uff08c\uff09<strong>1m<\/strong>\u4e0e\u7ec6\u80de\uff08HeLa\u548cMCF-7\u7ec6\u80de\u88c2\u89e3\u7269\uff09\u53cd\u5e94\u7684WB\u5206\u6790\u3002<\/p>\n<p>\uff08d\uff09\u4e0d\u540c\u6d53\u5ea6\uff082-10 mM\uff09\u4e0b<strong>1m<\/strong>\u548cHeLa\u7ec6\u80de\u88c2\u89e3\u7269\u4e4b\u95f4\u53cd\u5e94\u7684WB\u5206\u6790\u4ee5\u53ca\u534a\u80f1\u6c28\u9178\u53cd\u5e94\u6027IAM\u7684\u7ade\u4e89\u6027\u5206\u6790\u3002<\/p>\n<p>\uff08e\uff09\u86cb\u767d\u8d28\u7ec4\u4e2d\u6c28\u57fa\u9178\u9009\u62e9\u6027\u7684\u8868\u5f81\u3002<\/p>\n<p>\uff08f\uff09\u901a\u8fc7<strong>1m<\/strong>\u7684\u5171\u6709\u57fa\u5e8f\u8bc6\u522b\u3002<\/p>\n<p>\uff08g\uff09\u86cb\u767d\u8d28\u7ec4\u5b66\u7814\u7a76\u4e2d\u9274\u5b9a\u7684<strong>1m<\/strong>\u6807\u8bb0RPL37\u80bd\u7684\u6ce8\u91caMS2\u3002<\/p>\n<p><strong>\u4e94\u3001\u3010\u6210\u679c\u542f\u793a\u3011<\/strong><\/p>\n<p>\u7efc\u4e0a\u6240\u8ff0\uff0c\u7814\u7a76\u4eba\u5458\u62a5\u9053\u4e86\u4e00\u79cd\u6838\u9ec4\u7d20\u884d\u751f\u7269\u50ac\u5316\u7684\u786b\u9187-\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u5149\u70b9\u51fb\u53cd\u5e94\uff0c\u8be5\u53cd\u5e94\u80fd\u591f\u5b9e\u73b0Cys\u7279\u5f02\u6027\u751f\u7269\u5076\u8054\uff0c\u540c\u65f6\u5177\u6709\u8f83\u597d\u7684\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u65e0\u9700\u91d1\u5c5e\u50ac\u5316\u5c31\u80fd\u5b9e\u73b0\u5feb\u901f\u53cd\u5e94\u52a8\u529b\u5b66\u3002\u8fd9\u79cd\u4e0d\u542b\u91d1\u5c5e\u4e14\u9ad8\u6548\u7684\u53ef\u89c1\u5149\u4fc3\u8fdb\u7684\u6c27\u5316\u786b\u53f6\u7acb\u5fb7\u6d3b\u5316\u53cd\u5e94\u7528\u4e8e\u86cb\u767d\u8d28Cys\u6b8b\u57fa\u9009\u62e9\u5316\u5b66\u4fee\u9970\u65b9\u6cd5\uff0c\u4e3a\u57fa\u4e8e\u86cb\u767d\u8d28\u5316\u5b66\u4fee\u9970\u65b9\u6cd5\u5b66\u7684\u5316\u5b66\u751f\u7269\u5b66\u548c\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u7814\u7a76\u589e\u6dfb\u4e86\u4e00\u7c7b\u5177\u6709\u5e94\u7528\u6f5c\u529b\u7684\u65b0\u5de5\u5177\u3002<\/p>\n<p>\u6587\u732e\u94fe\u63a5\uff1a<a href=\"https:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2023\/SC\/D2SC05650J\" target=\"_blank\" rel=\"noopener\">The thiol-sulfoxonium ylide photo-click reaction for bioconjugation<\/a> (<em>Chem. Sci. <\/em><strong>2022<\/strong>, DOI: 10.1039\/D2SC05650J)<\/p>\n<p>&nbsp;<\/p>\n<p>\u672c\u6587\u7531\u5927\u5175\u54e5\u4f9b\u7a3f<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011 \u86cb\u767d\u8d28\u5316\u5b66\u4fee\u9970\u5de5\u5177\u7684\u5f00\u53d1\u4fc3\u8fdb\u4e86\u5316\u5b66\u751f\u7269\u5b66\u548c\u836f\u5b66\u7814\u7a76\u7684\u8fdb\u6b65\u3002\u5176\u4e2d\uff0c\u86cb\u767d\u8d28\u534a\u80f1\u6c28\u9178\uff08Cys\uff09\u6b8b\u57fa\u7684\u9009\u62e9\u6027\u4fee\u9970\u53d1\u6325\u7740\u6781\u4e3a\u91cd\u8981\u7684\u4f5c\u7528\u3002\u8fd1\u5e74\u6765\uff0c\u7814\u7a76\u8005\u5bf9\u53ef\u89c1&#8230;<\/p>\n","protected":false},"author":1004,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[],"class_list":["post-245365","post","type-post","status-publish","format-standard","hentry","category-jiedu"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/245365"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/1004"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=245365"}],"version-history":[{"count":3,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/245365\/revisions"}],"predecessor-version":[{"id":245389,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/245365\/revisions\/245389"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=245365"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=245365"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=245365"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}