{"id":265789,"date":"2025-03-28T09:20:38","date_gmt":"2025-03-28T01:20:38","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=265789"},"modified":"2025-03-28T09:20:38","modified_gmt":"2025-03-28T01:20:38","slug":"%e5%8c%97%e4%ba%ac%e5%a4%a7%e5%ad%a6science%ef%bc%9a%e5%8d%95%e7%bb%86%e8%83%9enanoatac-seq2%e6%8f%ad%e7%a4%ba%e5%b0%8f%e9%bc%a0%e6%97%a9%e6%9c%9f%e8%83%9a%e8%83%8e%e6%9f%93%e8%89%b2%e8%b4%a8%e5%8f%af","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=265789","title":{"rendered":"\u5317\u4eac\u5927\u5b66Science\uff1a\u5355\u7ec6\u80deNanoATAC-seq2\u63ed\u793a\u5c0f\u9f20\u65e9\u671f\u80da\u80ce\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u666f\u89c2"},"content":{"rendered":"<p><strong>\u4e00\u3001\u3010\u79d1\u5b66\u80cc\u666f\u3011<\/strong><\/p>\n<p>\u54fa\u4e73\u52a8\u7269\u7684\u65e9\u671f\u80da\u80ce\u53d1\u80b2\uff08\u4ece\u53d7\u7cbe\u5375\u5230\u690d\u5165\u524d\u80da\u80ce\uff09\u662f\u751f\u547d\u8d77\u59cb\u7684\u5173\u952e\u9636\u6bb5\uff0c\u8fd9\u4e00\u65f6\u671f\u80da\u80ce\u4f1a\u7ecf\u5386\u57fa\u56e0\u7ec4\u6fc0\u6d3b\u3001\u7ec6\u80de\u8c31\u7cfb\u5206\u5316\u4ee5\u53ca\u591a\u80fd\u6027\u5efa\u7acb\u7b49\u91cd\u8981\u4e8b\u4ef6\u3002\u7136\u800c\uff0c\u7531\u4e8e\u6280\u672f\u9650\u5236\uff0c\u5bf9\u4e8e\u8fd9\u4e00\u9636\u6bb5\u8868\u89c2\u9057\u4f20\u8c03\u63a7\u673a\u5236\u7684\u7406\u89e3\u4ecd\u4e0d\u5b8c\u5584\u3002\u8868\u89c2\u9057\u4f20\u8c03\u63a7\uff0c\u5c24\u5176\u662f\u67d3\u8272\u8d28\u72b6\u6001\u7684\u8c03\u63a7\uff0c\u5728\u57fa\u56e0\u8868\u8fbe\u3001\u7ec6\u80de\u547d\u8fd0\u51b3\u5b9a\u7b49\u65b9\u9762\u8d77\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4f8b\u5982\uff0c\u67d3\u8272\u8d28\u7684\u53ef\u53ca\u6027\u53d8\u5316\u4f1a\u5f71\u54cd\u8f6c\u5f55\u56e0\u5b50\u7684\u7ed3\u5408\u4ee5\u53ca\u57fa\u56e0\u7684\u8868\u8fbe\u3002\u6b64\u5916\uff0c\u5370\u8bb0\u57fa\u56e0\uff08\u57fa\u56e0\u8868\u8fbe\u53d7\u4eb2\u672c\u6765\u6e90\u5f71\u54cd\uff09\u5728\u80da\u80ce\u53d1\u80b2\u4e2d\u4e5f\u626e\u6f14\u7740\u5173\u952e\u89d2\u8272\uff0c\u5305\u62ec\u975e\u5178\u578b\u5370\u8bb0\u57fa\u56e0\uff08\u53d7\u6bcd\u672cH3K27me3\u4fee\u9970\u8c03\u63a7\u7684\u57fa\u56e0\uff09\u4ee5\u53ca\u4e0eX\u67d3\u8272\u4f53\u5931\u6d3b\uff08XCI\uff09\u76f8\u5173\u7684\u5370\u8bb0\u8c03\u63a7\u3002\u56e0\u6b64\uff0c\u6df1\u5165\u7814\u7a76\u65e9\u671f\u80da\u80ce\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7684\u67d3\u8272\u8d28\u72b6\u6001\u53ca\u5176\u52a8\u6001\u53d8\u5316\uff0c\u5bf9\u4e8e\u7406\u89e3\u80da\u80ce\u53d1\u80b2\u7684\u5206\u5b50\u673a\u5236\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n<p><strong>\u4e8c\u3001\u3010\u521b\u65b0\u6210\u679c\u3011<\/strong><\/p>\n<p>\u8fd1\u671f\uff0c<strong>\u5317\u4eac\u5927\u5b66\u6c64\u5bcc\u916c\u6559\u6388\u548c<\/strong><strong>Kehkooi Kee<\/strong><strong>\u6559\u6388<\/strong>\u8054\u5408\u5728<strong><em>Science<\/em><\/strong>\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u201c<strong>Chromatin accessibility landscape of mouse early embryos revealed by single-cell NanoATAC-seq2<\/strong>\u201d\u7684\u8bba\u6587\uff0c\u62a5\u9053\u5f00\u53d1\u4e86\u5f00\u53d1\u4e86scNanoATAC-seq2\u6280\u672f\uff0c\u8fd9\u662f\u4e00\u79cd\u4f7f\u7528\u7a00\u7f3a\u6837\u672c\u7684\u957f\u8bfb\u957f\u6d4b\u5e8f\u5bf9\u8f6c\u5ea7\u9176\u53ef\u53ca\u67d3\u8272\u8d28\u8fdb\u884c\u5355\u7ec6\u80de\u68c0\u6d4b\u7684\u65b9\u6cd5\uff0c\u5e76\u5229\u7528\u8be5\u65b9\u6cd5\u5206\u6790\u5c0f\u9f20\u7740\u5e8a\u524d\u53d1\u80b2\u5404\u5173\u952e\u9636\u6bb5\u7684\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u666f\u89c2\uff0c\u63a2\u7a76\u8f6c\u5f55\u56e0\u5b50\u5728\u5176\u4e2d\u7684\u4f5c\u7528\u673a\u5236\u3002\u8be5\u6280\u672f\u901a\u8fc7\u4f18\u5316\u5355\u7ec6\u80de\u5206\u79bb\u3001\u6838\u63d0\u53d6\u3001Tn5\u8f6c\u5ea7\u9176\u5904\u7406\u7b49\u6b65\u9aa4\uff0c\u5e76\u7ed3\u5408Oxford Nanopore\u6d4b\u5e8f\u5e73\u53f0\uff0c\u5b9e\u73b0\u4e86\u5bf9\u7a00\u7f3a\u6837\u672c\uff08\u5982\u5355\u4e2a\u7ec6\u80de\uff09\u7684\u9ad8\u5206\u8fa8\u7387\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u5206\u6790\u3002\u7814\u7a76\u4eba\u5458\u5229\u7528\u8be5\u6280\u672f\u5bf9\u5c0f\u9f20\u4ece\u5408\u5b50\u5230\u56ca\u80da\u5404\u4e2a\u9636\u6bb5\u7684\u80da\u80ce\u8fdb\u884c\u4e86\u5206\u6790\uff0c\u5171\u83b7\u5f97\u4e863302\u4e2a\u5355\u7ec6\u80de\u7684\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u56fe\u8c31\u3002\u672c\u7814\u7a76\u4e3a\u54fa\u4e73\u52a8\u7269\u690d\u5165\u524d\u53d1\u80b2\u548c\u8c31\u7cfb\u5206\u5316\u8fc7\u7a0b\u4e2d\u67d3\u8272\u8d28\u52a8\u529b\u5b66\u63d0\u4f9b\u4e86\u89c1\u89e3\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265790\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/0-4.png\" alt=\"\" width=\"600\" height=\"265\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/0-4.png 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/0-4-300x133.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u4e09\u3001\u3010\u56fe\u6587\u89e3\u6790\u3011<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265791\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/1-6.jpg\" alt=\"\" width=\"600\" height=\"778\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/1-6.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/1-6-231x300.jpg 231w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>1\u00a0 scNanoATAC-seq2<\/strong><strong>\u5206\u6790\u5c0f\u9f20\u7740\u5e8a\u524d\u80da\u80ce\u67d3\u8272\u8d28\u53ef\u53ca\u6027<\/strong><strong> \u00a9 2025 AAAS<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265792\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/2-5.jpg\" alt=\"\" width=\"600\" height=\"761\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/2-5.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/2-5-237x300.jpg 237w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>2\u00a0 <\/strong><strong>\u6b21\u8981<\/strong><strong>ZGA<\/strong><strong>\u548c\u4e3b\u8981<\/strong><strong>ZGA<\/strong><strong>\u7684\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u52a8\u6001<\/strong> <strong>\u00a9 2025 AAAS<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265793\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/3-6.jpg\" alt=\"\" width=\"600\" height=\"797\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/3-6.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/3-6-226x300.jpg 226w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>3\u00a0 <\/strong><strong>\u5c0f\u9f20\u7740\u5e8a\u524d\u53d1\u80b2\u4e2d\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u7684\u987a\u5f0f\u8c03\u63a7\u52a8\u529b\u5b66\u53ca\u5176\u5728\u7ec6\u80de\u547d\u8fd0\u51b3\u5b9a\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528<\/strong><strong> \u00a9 2025 AAAS<\/strong><\/p>\n<p><strong>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265794\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/4-7.jpg\" alt=\"\" width=\"600\" height=\"809\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/4-7.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/4-7-222x300.jpg 222w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p><strong>\u56fe<\/strong><strong>4\u00a0 <\/strong><strong>\u5e38\u67d3\u8272\u4f53\u975e\u7ecf\u5178\u5370\u8bb0\u57fa\u56e0\u548c\u5370\u8bb0<\/strong><strong>X<\/strong><strong>\u67d3\u8272\u4f53\u5931\u6d3b\u7684\u7b49\u4f4d\u57fa\u56e0\u7279\u5f02\u6027\u5f00\u653e\u67d3\u8272\u8d28\u666f\u89c2<\/strong><strong> \u00a9 2025 AAAS<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265795\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/5-4.jpg\" alt=\"\" width=\"600\" height=\"802\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/5-4.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/5-4-224x300.jpg 224w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>5\u00a0 <\/strong><strong>\u5c0f\u9f20\u690d\u5165\u524d\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u91cd\u590d\u5143\u4ef6\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u7684\u52a8\u529b\u5b66<\/strong><strong> \u00a9 2025 AAAS<\/strong><\/p>\n<p><strong>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-265796\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/6.jpg\" alt=\"\" width=\"600\" height=\"590\" srcset=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/6.jpg 600w, http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/03\/6-300x295.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p><strong>\u56fe<\/strong><strong>6\u00a0 Zscan4<\/strong><strong>\u540c\u6e90\u7269\u5728\u5c0f\u9f20\u665a\u671f\u4e8c\u7ec6\u80de\u80da\u80ce\u4e2d\u7684\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u548c<\/strong><strong>RNA<\/strong><strong>\u8868\u8fbe<\/strong><strong> \u00a9 2025 AAAS<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u56db\u3001\u3010\u79d1\u5b66\u542f\u8fea\u3011<\/strong><\/p>\n<p>\u7efc\u4e0a\uff0c\u672c\u7814\u7a76\u5f00\u53d1\u4e86scNanoATAC-seq2\uff0c\u53ef\u7528\u4e8e\u5206\u6790\u5373\u4f7f\u53ea\u6709\u4e00\u4e2a\u7ec6\u80de\u7684\u7a00\u7f3a\u6837\u672c\u7684\u7814\u7a76\u3002\u901a\u8fc7\u8fd9\u79cd\u65b9\u6cd5\uff0c\u7814\u7a76\u4eba\u5458\u9996\u6b21\u7cfb\u7edf\u63ed\u793a\u5c0f\u9f20\u690d\u5165\u524d\u53d1\u80b2\u4e2d\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u7684\u5168\u666f\u89c2\uff0c\u586b\u8865\u4e86\u8c31\u7cfb\u5206\u5316\u548cXCI\u52a8\u6001\u7684\u7a7a\u767d\uff0c\u5e76\u63d0\u51fa\u91cd\u590d\u5143\u4ef6\u901a\u8fc7\u987a\u5f0f\u8c03\u63a7\u53c2\u4e0eZGA\u548c\u80da\u80ce\u53d1\u80b2\u7684\u65b0\u673a\u5236\u3002\u8be5\u65b9\u6cd5\u53ef\u4f5c\u4e3a\u7814\u7a76\u73cd\u8d35\u548c\u7a00\u6709\u6837\u672c\u7684\u5de5\u5177\u3002\u5355\u7ec6\u80de\u56fe\u8c31\u4e3a\u7b2c\u4e00\u8c31\u7cfb\u5206\u5316\uff08ICM\u548c\u65e9\u671fTE\u4e4b\u95f4\uff09\u548c\u7b2c\u4e8c\u8c31\u7cfb\u5206\u5316\uff08EPI\u548cPE\u4e4b\u95f4\uff09\u7684\u8868\u89c2\u9057\u4f20\u8c03\u63a7\u63d0\u4f9b\u4e86\u6709\u4ef7\u503c\u7684\u89c1\u89e3\uff0c\u5e76\u4e3a\u672a\u6765\u7814\u7a76\u54fa\u4e73\u52a8\u7269\u690d\u5165\u524d\u53d1\u80b2\u7684\u8868\u89c2\u9057\u4f20\u5b66\u673a\u5236\u94fa\u5e73\u4e86\u9053\u8def\u3002<\/p>\n<p>\u539f\u6587\u8be6\u60c5\uff1a<a href=\"https:\/\/www.science.org\/doi\/10.1126\/science.adp4319\" target=\"_blank\" rel=\"noopener\">Chromatin accessibility landscape of mouse early embryos revealed by single-cell NanoATAC-seq2<\/a> (<em>Science<\/em> <strong>2025<\/strong>, 387, DOI: 10.1126\/science.adp4319)<\/p>\n<p>\u672c\u6587\u7531\u5927\u5175\u54e5\u4f9b\u7a3f\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u3010\u79d1\u5b66\u80cc\u666f\u3011 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