{"id":269270,"date":"2025-11-05T09:42:20","date_gmt":"2025-11-05T01:42:20","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=269270"},"modified":"2025-11-05T09:42:20","modified_gmt":"2025-11-05T01:42:20","slug":"%e6%b5%8e%e5%8d%97%e5%a4%a7%e5%ad%a6%e5%bc%a0%e4%b8%9b%e4%b8%9b-%e5%88%98%e5%ae%8f%e6%95%99%e6%8e%88%e5%8c%bb%e5%b7%a5%e4%ba%a4%e5%8f%89%e5%9b%a2%e9%98%9f%ef%bc%9a%e7%ba%b3%e7%b1%b3%e9%a2%97%e7%b2%92","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=269270","title":{"rendered":"\u6d4e\u5357\u5927\u5b66\u5f20\u4e1b\u4e1b\/\u5218\u5b8f\u6559\u6388\u533b\u5de5\u4ea4\u53c9\u56e2\u961f\uff1a\u7eb3\u7c73\u9897\u7c92\u5185\u541e\u4ecb\u5bfc\u7684\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u89e6\u53d1\u7ebf\u7c92\u4f53\u4fee\u590d\u51b3\u5b9a\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u52a0\u901f\u7cd6\u5c3f\u75c5\u4f24\u53e3\u6108\u5408"},"content":{"rendered":"\n<p>\u6d88\u9664\u6d3b\u6027\u6c27\u7c07\uff08ROS\uff09\u548c\u8bf1\u5bfc\u6297\u708e\u578bM2\u578b\u5de8\u566c\u7ec6\u80de\u6781\u5316\u5bf9\u7ec4\u7ec7\u4fee\u590d\u81f3\u5173\u91cd\u8981\u3002\u7136\u800c\uff0c\u5728\u4f53\u5185\u7cbe\u786e\u8c03\u63a7\u8fd9\u4e9b\u8fc7\u7a0b\u6781\u5177\u6311\u6218\u6027\u3002\u5c3d\u7ba1\u5c0f\u7684\u751f\u7269\u5206\u5b50\u548c\u5177\u6709ROS\u6e05\u9664\u548cM2\u6781\u5316\u7535\u4f4d\u7684\u91d1\u5c5e\u79bb\u5b50\uff0c\u4f46\u5b83\u4eec\u7684\u9ad8\u6eb6\u89e3\u6027\u963b\u788d\u4e86\u7cbe\u786e\u9012\u9001\uff0c\u9650\u5236\u4e86\u6cbb\u7597\u6548\u679c\u3002<\/p>\n\n\n\n<p>\u8fd1\u65e5\uff0c\u6d4e\u5357\u5927\u5b66\u524d\u6cbf\u4ea4\u53c9\u79d1\u5b66\u7814\u7a76\u9662\u5f20\u4e1b\u4e1b\u3001\u5218\u5b8f\u6559\u6388\u4e0e\u5c71\u4e1c\u7b2c\u4e00\u533b\u79d1\u5927\u5b66\u8f9b\u6d9b\u6559\u6388\u5408\u4f5c\uff0c\u901a\u8fc7\u836f\u7269\u5206\u5b50\u548c\u79bb\u5b50\u7684\u7eb3\u7c73\u5316\uff0c\u6784\u5efa\u4e86pH\u54cd\u5e94\u7684\u7eff\u539f\u9178-\u950c\u7eb3\u7c73\u9897\u7c92\uff08CHA-Zn NPs\uff09\u3002\u8be5\u7eb3\u7c73\u9897\u7c92\u5229\u7528\u5de8\u566c\u7ec6\u80de\u7684\u5185\u541e\u4f5c\u7528\u548c\u6eb6\u9176\u4f53\u7684\u9178\u6027\uff0c\u5728\u5185\u5316\u540e3\u5c0f\u65f6\u5185\u89e6\u53d1\u7cbe\u786e\u7684\u201c\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u201d\uff0c\u6709\u6548\u5730\u6e05\u9664ROS\u548c\u4fee\u590d\u53d7\u635f\u7684\u7ebf\u7c92\u4f53\uff0c\u7279\u5f02\u6027\u6291\u5236NF-\u03baB p65\u7684\u78f7\u9178\u5316\uff0c\u540c\u65f6\u6fc0\u6d3b\u5de8\u566c\u7ec6\u80de\u5185\u7684STAT6\u4fe1\u53f7\u901a\u8def\u3002\u8fd9\u4e00\u53cc\u91cd\u4f5c\u7528\u5c06\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u4e3aM2\u8868\u578b\uff0c\u6062\u590d\u4f24\u53e3\u5fae\u73af\u5883\u4e2d\u7684\u514d\u75ab\u7a33\u6001\u3002\u4e3a\u8c03\u63a7\u5de8\u566c\u7ec6\u80de\u548c\u7ec4\u7ec7\u4fee\u590d\u63d0\u4f9b\u4e86\u4e00\u79cd\u666e\u904d\u9002\u7528\u7684\u7eb3\u7c73\u836f\u7269\u8bbe\u8ba1\u65b9\u6cd5\u3002\u7814\u7a76\u6210\u679c\u4ee5\u201cNanoparticle Endocytosis Mediated Intracellular Ion\/Small Molecule Storm Triggered Mitochondrial Repair Determined Macrophage Reprogramming for Accelerate Diabetic Wound Healing\u201d\u4e3a\u9898\u53d1\u8868\u5728\u56fd\u9645\u671f\u520aAdvanced Functional Materials (IF = 19)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"317\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/2382c315a6ba396-5.png\" alt=\"\" class=\"wp-image-269271\"\/><\/figure>\n\n\n\n<p>\u5218\u5b8f\u6559\u6388\uff0c\u5f20\u4e1b\u4e1b\u6559\u6388\u533b\u5de5\u4ea4\u53c9\u56e2\u961f\u7684\u7855\u58eb\u7814\u7a76\u751f\u5362\u5c71\uff0c\u8c22\u535a\u94a7\u548c\u5c71\u4e1c\u7b2c\u4e00\u533b\u79d1\u5927\u5b66\u7684\u82cf\u7433\u6dc7\u4e3a\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002\u5f20\u4e1b\u4e1b\u6559\u6388\u3001\u5218\u5b8f\u6559\u6388\u3001\u5c71\u4e1c\u7b2c\u4e00\u533b\u79d1\u5927\u5b66\u7684\u8f9b\u6d9b\u6559\u6388\u4e3a\u5171\u540c\u901a\u8baf\u4f5c\u8005\u3002\u539f\u6587\u94fe\u63a5\uff1a<a href=\"https:\/\/doi.org\/10.1002\/adfm.202520004\"><u>https:\/\/doi.org\/10.1002\/adfm.202520004<\/u><\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"538\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/ce6e2814f207c93-6.png\" alt=\"\" class=\"wp-image-269272\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>1. CHA-Zn NPs<\/strong><strong>\u4fc3\u8fdb\u7cd6\u5c3f\u75c5\u4f24\u53e3\u6108\u5408\u7684\u673a\u5236\u793a\u610f\u56fe<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>\u4e3b\u8981\u5185\u5bb9<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>1. CHA-Zn NPs<\/strong><strong>\u7684\u5236\u5907\u4e0e\u8868\u5f81<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u7814\u7a76\u56e2\u961f\u91c7\u7528\u4e86\u4e00\u6b65\u5236\u5907\u7b56\u7565\u901a\u8fc7\u5c06\u7eff\u539f\u9178\uff08\u5929\u7136\u690d\u7269\u63d0\u53d6\u7269\uff09\u4e0e\u950c\u79bb\u5b50\uff08Zn<sup>2+<\/sup>\uff09\u76f8\u7ed3\u5408\uff0c\u5408\u6210\u4e86\u7eff\u539f\u9178-\u950c\u7eb3\u7c73\u9897\u7c92\uff08CHA-Zn NPs\uff09\uff0c\u5f00\u53d1\u4e00\u79cd\u5177\u6709\u201c\u6d3b\u6027\u6c27\u6e05\u9664\u2014\u7ebf\u7c92\u4f53\u4fee\u590d\u2014\u5de8\u566c\u7ec6\u80de\u6781\u5316\u8c03\u63a7\u201d\u534f\u540c\u673a\u5236\u7684\u521b\u65b0\u578b\u7eb3\u7c73\u836f\u7269\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"749\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/7f88dbbcaa77a1d-5.png\" alt=\"\" class=\"wp-image-269273\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>2. CHA-Zn NPs<\/strong><strong>\u7684\u5236\u5907\u4e0e\u8868\u5f81<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>2. <\/strong><strong>\u4f53\u5916<\/strong><strong>\u91cd\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\u8868\u578b<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cf\u9006\u8f6c\u5f55\u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08RT-qPCR\uff09\u68c0\u6d4b\u4fc3\u708eM1\u578b\u57fa\u56e0\uff08\u8bf1\u5bfc\u578b\u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08iNOS\uff09\u3001\u80bf\u7624\u574f\u6b7b\u56e0\u5b50-\u03b1\uff08TNF-\u03b1\uff09\u548cCD86\uff09\u4ee5\u53ca\u6297\u708eM2\u578b\u57fa\u56e0\uff08\u7cbe\u6c28\u9178\u91761\uff08Arg1\uff09\u3001\u8f6c\u5316\u751f\u957f\u56e0\u5b50-\u03b21\uff08TGF-\u03b21\uff09\u548cCD206\uff09\u7684\u8f6c\u5f55\u6c34\u5e73\uff0c\u4ee5\u9a8c\u8bc1CHA-Zn NPs\u7684\u6297\u708e\u7279\u6027\u3002qPCR\uff0c\u514d\u75ab\u8367\u5149\u67d3\u8272\uff0cWestern blot\u7ed3\u679c\u8868\u660e\uff0c\u5728\u6dfb\u52a0CHA-Zn\u540e\uff0cM1\u578b\u6807\u5fd7\u7269\u7684\u8868\u8fbe\u6c34\u5e73\u663e\u8457\u964d\u4f4e\uff1b\u540c\u65f6M2\u578b\u6807\u5fd7\u7269\u7684\u8868\u8fbe\u5448\u73b0\u51fa\u4e0a\u5347\u8d8b\u52bf\u3002\u8bf4\u660eCHA-Zn NPs\u53ef\u4ee5\u91cd\u65b0\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\uff0c\u5c06\u708e\u75c7\u7684M1\u578b\u6781\u5316\u8f6c\u53d8\u4e3a\u6297\u708e\u7684M2\u578b\uff0c\u6709\u52a9\u4e8e\u5efa\u7acb\u6709\u5229\u4e8e\u6291\u5236\u708e\u75c7\u548c\u4fc3\u8fdb\u7ec4\u7ec7\u4fee\u590d\u7684\u5fae\u73af\u5883\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"770\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/4e9a0ba2491dfde-5.png\" alt=\"\" class=\"wp-image-269274\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>3. CHA-Zn NPs<\/strong><strong>\u91cd\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\u8868\u578b<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>3. \u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u7814\u7a76\u56e2\u961f\u8fdb\u4e00\u6b65\u7814\u7a76\u4e86CHA-Zn NPs\u5728\u5de8\u566c\u7ec6\u80de\u5185\u7684\u91cd\u7f16\u7a0b\u673a\u5236\uff0c\u9ad8\u5206\u8fa8\u7387\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\uff08HR-TEM\uff09\u663e\u793a\uff0c\u9664\u4e86\u5c11\u91cf\u9897\u7c92\u9644\u7740\u5728\u7ec6\u80de\u819c\u8868\u9762\u5916\uff0c\u5927\u91cfCHA-Zn NPs\u5728\u6eb6\u9176\u4f53\u4e2d\u5bc6\u96c6\u805a\u96c6\u3002\u8fdb\u4e00\u6b65\u4f7f\u7528\u8367\u5149\u7d20\u5f02\u786b\u6c30\u9178\u916f\uff08FITC\uff09\u6807\u8bb0\u7684\u7eb3\u7c73\u9897\u7c92\uff08\u7eff\u8272\uff09\u4e0e\u5de8\u566c\u7ec6\u80de\u8fdb\u884c\u5171\u57f9\u517b\uff0c\u8fdb\u884c\u6eb6\u9176\u4f53\u67d3\u8272\u3002\u5728\u5b75\u80b23\u5c0f\u65f6\u540e\uff0c\u7eb3\u7c73\u9897\u7c92\u5728\u7ec6\u80de\u5185\u7684\u5206\u5e03\u8fbe\u5230\u6700\u5927\u503c\uff0c\u8367\u5149\u5f3a\u5ea6\u4e5f\u8fbe\u5230\u6700\u5927\u503c\u3002\u968f\u7740\u5b75\u80b2\u65f6\u95f4\u7684\u5ef6\u957f\uff0c\u8367\u5149\u4fe1\u53f7\u51cf\u5f31\uff0c\u8fd9\u8868\u660e\u5185\u5316\u7684\u7eb3\u7c73\u9897\u7c92\u9996\u5148\u5728\u6eb6\u9176\u4f53\u4e2d\u79ef\u7d2f\uff0c\u968f\u540e\u5728\u7ec6\u80de\u5185\u53d1\u751f\u9010\u6b65\u7684\u89e3\u79bb\u3002\u7535\u611f\u8026\u5408\u7b49\u79bb\u5b50\u4f53\u8d28\u8c31\u6cd5\uff08ICP-MS\uff09\u5728\u7ec6\u80de\u5916\u6a21\u62df\u4e86CHA-Zn NPs\u4e2d\u950c\u79bb\u5b50\u7684\u91ca\u653e\u884c\u4e3a\uff0c\u5728pH\u22485\uff0cCHA-Zn\u57286\u5c0f\u65f6\u5185\u8fc5\u901f\u5206\u89e3\uff0c\u5f15\u53d1\u950c\u79bb\u5b50\u7684\u7206\u53d1\u6027\u91ca\u653e\u2014\u201c\u950c\u79bb\u5b50\u98ce\u66b4\u201d\u3002<\/p>\n\n\n\n<p>\u8fdb\u4e00\u6b65\u89c2\u5bdf\u7ec6\u80de\u5185Zn<sup>2+<\/sup>\u6d53\u5ea6\u968f\u65f6\u95f4\u7684\u53d8\u5316\u53ca\u5176\u4e0e\u6eb6\u9176\u4f53\u7684\u76f8\u5bf9\u4f4d\u7f6e\u3002\u5728\u4e0eCHA-Zn NPs\u5171\u57f9\u517b3\u5c0f\u65f6\u540e\uff0c\u4ec5\u68c0\u6d4b\u5230\u5fae\u5f31\u7684\u7eff\u8272\u8367\u5149\u4fe1\u53f7\uff0c\u8868\u660e\u5728\u65e9\u671f\u65f6\u95f4\u70b9\uff0c\u4ec5\u6709\u5c11\u91cf\u7eb3\u7c73\u9897\u7c92\u88ab\u6eb6\u9176\u4f53\u6d88\u5316\u3002\u5171\u57f9\u517b\u65f6\u95f4\u5ef6\u957f\u81f36\u5c0f\u65f6\u540e\uff0c\u89c2\u5bdf\u5230\u950c\u79bb\u5b50\u6709\u660e\u663e\u7684\u91cd\u65b0\u5206\u5e03\uff0c\u950c\u79bb\u5b50\u5728\u6eb6\u9176\u4f53\u5468\u56f4\u6709\u663e\u8457\u7684\u805a\u96c6\u3002\u8868\u660eCHA-Zn NPs\u5728\u5927\u7ea63\u5c0f\u65f6\u7684\u6eb6\u9176\u4f53\u6d88\u5316\u540e\u5f00\u59cb\u964d\u89e3\u5e76\u91ca\u653e\u950c\u79bb\u5b50\u3002\u572812\u5c0f\u65f6\uff0c\u950c\u79bb\u5b50\u4fe1\u53f7\u4e0e\u6eb6\u9176\u4f53\u4e4b\u95f4\u7684\u90e8\u5206\u7a7a\u95f4\u5206\u79bb\u53d8\u5f97\u660e\u663e\u3002\u523024\u5c0f\u65f6\uff0c\u89c2\u5bdf\u5230\u950c\u79bb\u5b50\u63a2\u9488\u4e0e\u6eb6\u9176\u4f53\u8ffd\u8e2a\u5668\u4e4b\u95f4\u6709\u660e\u663e\u7684\u5206\u79bb\uff0c\u8fd9\u8868\u660e\u91ca\u653e\u7684\u950c\u79bb\u5b50\u5df2\u79bb\u5f00\u6eb6\u9176\u4f53\u5e76\u5728\u7ec6\u80de\u8d28\u4e2d\u5e7f\u6cdb\u6269\u6563\u3002\u8fd9\u4e9b\u53d1\u73b0\u4e0d\u4ec5\u8bc1\u5b9e\u4e86CHA-Zn\u7eb3\u7c73\u9897\u7c92\u7684\u52a8\u6001\u6eb6\u9176\u4f53\u964d\u89e3\u8fc7\u7a0b\u4ee5\u53ca\u968f\u540e\u950c\u79bb\u5b50\u7684\u91ca\u653e\uff0c\u800c\u4e14\u8fd8\u7a81\u663e\u4e86\u950c\u79bb\u5b50\u5728\u7ec6\u80de\u5185\u7684\u5e7f\u6cdb\u5206\u5e03\u60c5\u51b5\uff0c\u5f3a\u8c03\u4e86\u5176\u6f5c\u5728\u7684\u751f\u7269\u6d3b\u6027\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"840\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/b14210e1ee30421-4.png\" alt=\"\" class=\"wp-image-269275\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>4.<\/strong><strong>&nbsp;\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4<\/strong><strong><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u6e05\u9664\u6d3b\u6027\u6c27\u548c\u6062\u590d\u7ebf\u7c92\u4f53\u7a33\u6001<\/strong><\/li>\n<\/ul>\n\n\n\n<p>\u708e\u75c7\u6761\u4ef6\u4e0b\uff0c\u7ec6\u80de\u5185\u5916\u4ea7\u751f\u7684\u8fc7\u91cfROS\u53ef\u635f\u5bb3\u7ec6\u80de\u547c\u5438\uff0c\u7834\u574f\u7ebf\u7c92\u4f53\u7ed3\u6784\uff0c\u5bfc\u81f4\u7ebf\u7c92\u4f53\u819c\u53bb\u6781\u5316\u3002MitoSOX\u6df1\u7ea2\u8272\u63a2\u9488\u67d3\u8272\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\u663e\u793a\uff0cLPS\u5904\u7406\u663e\u8457\u589e\u52a0M1\u578b\u5de8\u566c\u7ec6\u80de\u7ebf\u7c92\u4f53\u6d3b\u6027\u6c27\uff08mtROS\uff09\u7684\u4ea7\u751f\uff0c\u800cCHA-Zn NPs\u5904\u7406\u663e\u8457\u964d\u4f4eM1\u578b\u5de8\u566c\u7ec6\u80demtROS\u7684\u6c34\u5e73\uff0c\u8868\u660e\u5728\u708e\u75c7\u6761\u4ef6\u4e0b\uff0cCHA-Zn NPs\u6709\u6548\u6e05\u9664\u7ebf\u7c92\u4f53ROS\u3002\u5728ROS\u8bf1\u5bfc\u7684\u6c27\u5316\u5e94\u6fc0\u4e2d\uff0cJC-1\u805a\u96c6\/\u5355\u4f53\u6bd4\u7387\u7684\u964d\u4f4e\u53cd\u6620\u4e86\u7ebf\u7c92\u4f53\u53bb\u6781\u5316\u548c\u529f\u80fd\u969c\u788d\u91c7\u7528JC-1\u63a2\u9488\u68c0\u6d4b\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\uff08MMP\uff09\uff1b\u8367\u5149\u67d3\u8272\u663e\u793a\uff0cLPS\u5904\u7406\u540e\uff0c\u7ea2\u8272\u8367\u5149\u51cf\u5c11\uff0c\u7eff\u8272\u8367\u5149\u589e\u52a0\uff0c\u63d0\u793a\u7ebf\u7c92\u4f53\u53bb\u6781\u5316\u548c\u635f\u4f24\u3002CHA-Zn NPs\u5904\u7406\u540e\u5bfc\u81f4\u7eff\u8272\u8367\u5149\u5f3a\u5ea6\u663e\u8457\u964d\u4f4e\uff0c\u7ea2\u8272\u8367\u5149\u76f8\u5e94\u589e\u52a0\u3002\u5b9a\u91cf\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u8fdb\u4e00\u6b65\u8bc1\u5b9e\uff0cCHA-Zn NPs\u90e8\u5206\u6062\u590d\u4e86LPS\u8bf1\u5bfc\u7684\u708e\u75c7\u5bfc\u81f4\u7684\u7ebf\u7c92\u4f53\u819c\u7535\u4f4d\u4e0b\u964d\u3002<\/p>\n\n\n\n<p>Mito\u7eff\u8272\u63a2\u9488\u663e\u793aLPS\u7ec4\u7ebf\u7c92\u4f53\u80bf\u80c0\uff0c\u7ed3\u6784\u6a21\u7cca\uff0c\u5448\u56ca\u6ce1\u5f62\u6001\u3002\u4e0e\u6b64\u76f8\u53cd\uff0cCHA-Zn NPs\u663e\u8457\u51cf\u8f7b\u4e86LPS\u8bf1\u5bfc\u7684\u7ebf\u7c92\u4f53\u5f62\u6001\u548c\u7f51\u7edc\u8fde\u63a5\u635f\u4f24\uff0c\u7ebf\u7c92\u4f53\u5448\u73b0\u51fa\u6e05\u6670\u7684\u4e1d\u72b6\u5206\u652f\u3002LPS+CHA-Zn\u7ec4\u7684\u5e73\u5747\u5206\u652f\u6570\u91cf\u548c\u5206\u652f\u5206\u652f\u8fde\u63a5\u6570\u91cf\u5747\u663e\u8457\u589e\u52a0\u3002TEM\u56fe\u50cf\u663e\u793aLPS\u8bf1\u5bfc\u7684M1\u5de8\u566c\u7ec6\u80de\u7ebf\u7c92\u4f53\u51fa\u73b0\u4e86\u4e25\u91cd\u7684\u7ed3\u6784\u635f\u4f24\uff0c\u8868\u73b0\u4e3a\u57fa\u8d28\u80bf\u80c0\u3001\u5d74\u65ad\u88c2\u3001\u5185\u90e8\u7a7a\u6ce1\u5316\u548c\u5185\u8d28\u7f51\uff08ER\uff09\u53d8\u6027\u3002\u4e0e\u5bf9\u7167\u7ec4\uff08Ctrl\uff09\u76f8\u6bd4\uff0c\u7ec6\u80de\u7ebf\u7c92\u4f53\u5e73\u5747\u957f\u5ea6\u51cf\u5c11\uff0c\u53d7\u635f\u7ebf\u7c92\u4f53\u6bd4\u4f8b\u589e\u52a0\u3002\u800cCHA-Zn NPs\u663e\u8457\u4fdd\u7559\u4e86\u7ebf\u7c92\u4f53\u7684\u5b8c\u6574\u6027\uff0c\u51cf\u5c11\u4e86\u53d7\u635f\u7ebf\u7c92\u4f53\u7684\u6570\u91cf\uff0c\u5e76\u7ef4\u6301\u4e86\u6b63\u5e38\u7684ER\u5f62\u6001\u3002\u8bf4\u660eCHA-Zn NPs\u901a\u8fc7\u6e05\u9664\u8fc7\u91cf\u7684ROS\u6709\u6548\u5730\u4fdd\u62a4\u7ebf\u7c92\u4f53\u548cER\u7ed3\u6784\u514d\u53d7ROS\u8bf1\u5bfc\u7684\u635f\u4f24\u3002\u4e0a\u8ff0\u7ed3\u679c\u8fdb\u4e00\u6b65\u8868\u660e\uff0cCHA-Zn NPs\u5f15\u53d1\u7684\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u5177\u6709\u8f83\u5f3a\u7684ROS\u6e05\u9664\u80fd\u529b\uff0c\u7f13\u89e3\u7ebf\u7c92\u4f53\u529f\u80fd\u969c\u788d\u548c\u6062\u590d\u7ebf\u7c92\u4f53\u7a33\u6001\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"811\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/de5bcf7d8eaea98-3.png\" alt=\"\" class=\"wp-image-269276\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>5.<\/strong><strong>&nbsp;\u7ec6\u80de\u5185\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u6e05\u9664<\/strong><strong>ROS<\/strong><strong>\u5e76\u6062\u590d\u7ebf\u7c92\u4f53\u7a33\u6001<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>5<\/strong><strong>.<\/strong><strong>&nbsp;CHA-Zn NPs<\/strong><strong>\u6297\u708e\u673a\u5236\u7684<\/strong><strong>RNA-seq<\/strong><strong>\u5206\u6790<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u7814\u7a76\u56e2\u961f\u4f7f\u7528RNA-seq\u6280\u672f\u8fdb\u884c\u8f6c\u5f55\u8c31\u5206\u6790\u7814\u7a76CHA-Zn\u7eb3\u7c73\u7c92\u5728\u7ebf\u7c92\u4f53\u4fee\u590d\u548c\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u4e2d\u4f5c\u7528\u7684\u5206\u5b50\u673a\u5236\u3002\u53d1\u73b0\u708e\u75c7\u76f8\u5173\u57fa\u56e0\u5982\u73af\u6c27\u5408\u9176-2\uff08COX-2\uff09\u548c\u8d8b\u5316\u56e0\u5b50\uff08CCL2\uff09\u7684\u8868\u8fbe\u663e\u8457\u4e0b\u8c03\uff0c\u6297\u708e\u57fa\u56e0\u5982\u767d\u7ec6\u80de\u4ecb\u7d20-10\uff08IL-10\uff09\u3001\u6eb6\u9176\u4f53\u76f8\u5173\u57fa\u56e0\uff08ACP5\uff09\u548c\u91d1\u5c5e\u786b\u86cb\u767d\u57fa\u56e0\uff08MT1\uff09\u7684\u8868\u8fbe\u5448\u4e0a\u8c03\u8d8b\u52bf\u3002\u4eac\u90fd\u57fa\u56e0\u4e0e\u57fa\u56e0\u7ec4\u767e\u79d1\u5168\u4e66\uff08KEGG\uff09\u901a\u8def\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0c\u708e\u75c7\u76f8\u5173\u901a\u8def\u53d1\u751f\u663e\u8457\u53d8\u5316\uff0c\u3002IL-17\u3001TNF\u548c\u6eb6\u9176\u4f53\u4fe1\u53f7\u901a\u8def\u663e\u8457\u6539\u53d8\u3002\u8fdb\u4e00\u6b65\u7684\u57fa\u56e0\u672c\u4f53\uff08GO\uff09\u5bcc\u96c6\u5206\u6790\u663e\u793a\uff0c\u8fd9\u4e9b\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u4e3b\u8981\u5bcc\u96c6\u5728\u6709\u673a\u73af\u5316\u5408\u7269\u548c\u950c\u79bb\u5b50\u7684\u53cd\u5e94\u4e2d\uff0c\u5e76\u4e0a\u8c03STAT\u86cb\u767d\u78f7\u9178\u5316\u8fc7\u7a0b\uff0c\u4e0b\u8c03TNF\uff0cIL-1\u03b2\u548cIL-17\u7684\u8868\u8fbe\u548c\u7ecf\u5178NF-\u03baB\u4fe1\u53f7\u901a\u8def\u7684\u4f20\u5bfc\u3002Western blot\u5b9e\u9a8c\u8868\u660e\uff0c\u4e0eLPS\u7ec4\u76f8\u6bd4\uff0cCHA-Zn NPs\u663e\u8457\u4e0b\u8c03p-NF-\u03baB p65\u7684\u8868\u8fbe\uff0c\u800c\u663e\u8457\u4e0a\u8c03p-STAT6\u7684\u8868\u8fbe\uff0cp-STAT6\u7684\u8868\u8fbe\u4e0a\u8c03\u3002\u8fd9\u4e00\u7ed3\u679c\u5f3a\u70c8\u652f\u6301NF-\u03baB\u548cJAK-STAT\u4fe1\u53f7\u901a\u8def\u53c2\u4e0e\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u3002\u57fa\u4e8e\u4e0a\u8ff0\u5b9e\u9a8c\u7ed3\u679c\uff0c\u63d0\u51faCHA-Zn NPs\u8c03\u63a7\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u7684\u4e00\u79cd\u53ef\u80fd\u7684\u751f\u7269\u5b66\u673a\u5236\uff1aCHA-Zn NPs\u901a\u8fc7\u5de8\u566c\u7ec6\u80de\u7684\u5185\u541e\u4f5c\u7528\u8fdb\u5165\u7ec6\u80de\uff0c\u5e76\u5728\u6eb6\u9176\u4f53\u5185\u8fc5\u901f\u91ca\u653eCHA\u548cZn<sup>2+<\/sup>\uff0c\u5f62\u6210\u79bb\u5b50\/\u5c0f\u5206\u5b50\u98ce\u66b4\u3002\u8fd9\u4e9b\u79bb\u5b50\u548c\u5c0f\u5206\u5b50\u540c\u65f6\u6291\u5236NF-\u03baB p65\u7684\u78f7\u9178\u5316\uff0c\u6fc0\u6d3bSTAT6\u4fe1\u53f7\u901a\u8def\uff0c\u4fc3\u8fdb\u5de8\u566c\u7ec6\u80de\u7531M1\u8868\u578b\u5411M2\u8868\u578b\u8f6c\u53d8\uff0c\u5b9e\u73b0\u5de8\u566c\u7ec6\u80de\u91cd\u7f16\u7a0b\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"783\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/aa3023e0288002c-2.png\" alt=\"\" class=\"wp-image-269277\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>6. CHA-Zn NPs<\/strong><strong>\u6297\u708e\u673a\u5236\u7684<\/strong><strong>RNA-seq<\/strong><strong>\u5206\u6790<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>6<\/strong><strong>.<\/strong><strong>&nbsp;<\/strong><strong>CHA-Zn NPs<\/strong><strong>\u4fc3\u8fdb\u7cd6\u5c3f\u75c5\u521b\u9762\u4f53\u5916\u548c\u4f53\u5185\u6108\u5408<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u968f\u540e\u6df1\u5165\u7814\u7a76CHA-Zn NPs\u4fc3\u8fdb\u7cd6\u5c3f\u75c5\u5c0f\u9f20\u4f24\u53e3\u6108\u5408\u7684\u4f53\u5185\u7597\u6548\u3002\u901a\u8fc7\u9ad8\u7cd6\u9ad8\u8102\u996e\u98df\u8054\u5408\u94fe\u8132\u4f50\u83cc\u7d20\uff08STZ\uff09\u6ce8\u5c04\u5efa\u7acb\u7cd6\u5c3f\u75c5\u5c0f\u9f20\u6a21\u578b\u3002\u5c06\u521b\u9762\u5c0f\u9f20\u968f\u673a\u5206\u4e3a3\u7ec4\uff1a\u5bf9\u7167\u7ec4\uff08Ctrl\uff09\u3001GelMA\u6c34\u51dd\u80f6\u7ec4\uff08Gel\uff09\u3001\u6c34\u51dd\u80f6+CHA-Zn NPs\u7ec4\uff08Gel+CHA-Zn NPs\uff09\u3002\u5728\u7b2c0\u30013\u30015\u30017\u548c9\u65e5\u8fdb\u884c\u8089\u773c\u89c2\u5bdf\u3002\u7ed3\u679c\u8868\u660e\uff0c\u5230\u7b2c9\u5929\uff0cCtrl\u7ec4\u548cGel\u7ec4\u7684\u521b\u9762\u5c1a\u672a\u5b8c\u5168\u6108\u5408\u3002\u5176\u4e2d\uff0cGel+CHA-Zn NPs\u5904\u7406\u7ec4\u7684\u521b\u9762\u6108\u5408\u7387\u663e\u8457\u9ad8\u4e8e\u5176\u4ed6\u4e24\u7ec4\uff0c\u521b\u9762\u6108\u5408\u901f\u5ea6\u52a0\u5feb\u7ea610%\u3002<\/p>\n\n\n\n<p>\u82cf\u6728\u7cbe-\u4f0a\u7ea2\uff08H&amp;E\uff09\u67d3\u8272\u7ed3\u679c\u663e\u793a\uff0c\u5728\u7b2c5\u5929\uff0cCtrl\u7ec4\u548cGel\u7ec4\u76ae\u80a4\u7ec4\u7ec7\u51fa\u73b0\u660e\u663e\u51fa\u8840\u548c\u5e7f\u6cdb\u7684\u708e\u6027\u7ec6\u80de\u6d78\u6da6\u3002\u76f8\u53cd\uff0cCHA-Zn NPs\u5904\u7406\u7ec4\u708e\u75c7\u7ec6\u80de\u6d78\u6da6\u660e\u663e\u51cf\u5c11\u3002\u7b2c9\u5929\uff0cCtrl\u7ec4\u521b\u9762\u4ecd\u672a\u5b8c\u5168\u95ed\u5408\uff0c\u90e8\u5206\u708e\u6027\u7ec6\u80de\u6301\u7eed\u6d78\u6da6\uff1b\u800cGel+CHA-Zn NPs\u7ec4\u57fa\u672c\u6062\u590d\u6b63\u5e38\u6c34\u5e73\uff0c\u51e0\u4e4e\u6ca1\u6709\u708e\u6027\u7ec6\u80de\u6d78\u6da6\u3002\u9a6c\u677e\u4e09\u8272\u67d3\u8272\u5b9a\u91cf\u8bc4\u4f30\u80f6\u539f\u6c89\u79ef\u3002\u9a6c\u677e\u4e09\u8272\u67d3\u8272\u7ed3\u679c\u663e\u793a\uff0cGel+CHA-Zn NPs\u5904\u7406\u7684\u521b\u9762\u4e2d\u80f6\u539f\u6c89\u79ef\u660e\u663e\u9ad8\u4e8eCtrl\u7ec4\uff0c\u8868\u660eGel+CHA-Zn NPs\u53ef\u901a\u8fc7\u523a\u6fc0\u80f6\u539f\u7ea4\u7ef4\u7684\u5f62\u6210\u6709\u6548\u4fc3\u8fdb\u521b\u9762\u6108\u5408\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"801\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/e2f457ec632c1f6-1.png\" alt=\"\" class=\"wp-image-269278\"\/><\/figure>\n\n\n\n<p><strong>\u56fe7. CHA-Zn NPs\u5728\u4f53\u5185\u4fc3\u8fdb\u7cd6\u5c3f\u75c5\u5168\u5c42\u521b\u9762\u6108\u5408<\/strong><strong><\/strong><\/p>\n\n\n\n<p><strong>7<\/strong><strong>.<\/strong><strong>&nbsp;CHA-Zn NPs<\/strong><strong>\u91cd\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\u52a0\u901f\u4f53\u5185\u7cd6\u5c3f\u75c5\u521b\u9762\u6108\u5408<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u8fdb\u4e00\u6b65\u8bc4\u4f30\u5de8\u566c\u7ec6\u80de\u5728\u521b\u9762\u7ec4\u7ec7\u4e2d\u7684\u6d78\u6da6\uff0c\u5728\u4e0d\u540c\u65f6\u95f4\u70b9\u8fdb\u884c\u514d\u75ab\u8367\u5149\u67d3\u8272\u3002\u4e0eCtrl\u7ec4\u548cGel\u7ec4\u76f8\u6bd4\uff0cGel+CHA-Zn NPs\u7ec4M1\u578b\u5de8\u566c\u7ec6\u80de\u6d78\u6da6\uff08F4\/80\u548ciNOS\uff09\u660e\u663e\u51cf\u5c11\uff0cM2\u578b\u5de8\u566c\u7ec6\u80de\u6d78\u6da6\uff08F4\/80\u548cCD206\uff09\u660e\u663e\u589e\u52a0\u3002\u514d\u75ab\u7ec4\u5316\u7ed3\u679c\u663e\u793a\uff0c\u4e0eCtrl\u7ec4\u76f8\u6bd4\uff0cGel+CHA-Zn NPs\u7ec4\u5728\u7b2c5\u5929TNF-\u03b1\u7684\u8868\u8fbe\u660e\u663e\u51cf\u5c11\uff0c\u5230\u7b2c9\u5929\u51e0\u4e4e\u6062\u590d\u5230\u6b63\u5e38\u6c34\u5e73\u3002Western blot\u663e\u793a\uff0cGel+CHA-Zn NPs\u7ec4\u78f7\u9178\u5316NF-\u03baB p65\uff08p-NF-\u03baB p65\uff09\u8868\u8fbe\u4e0b\u8c03\uff0c\u78f7\u9178\u5316STAT6\uff08p-STAT6\uff09\u8868\u8fbe\u4e0a\u8c03\u3002\u4e0a\u8ff0\u5b9e\u9a8c\u7ed3\u679c\u8bc1\u5b9e\u4e86CHA-Zn NPs\u5728\u4f53\u5185\u8868\u73b0\u51fa\u826f\u597d\u7684\u751f\u7269\u76f8\u5bb9\u6027\u548c\u5f3a\u5927\u7684\u6297\u708e\u4f5c\u7528\u3002\u901a\u8fc7\u8c03\u8282NF-\u03baB p65\u548cSTAT6\u4fe1\u53f7\u901a\u8def\u548c\u91cd\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\u8868\u578b\uff0cCHA-Zn NPs\u6709\u6548\u6062\u590d\u4e86\u7cd6\u5c3f\u75c5\u521b\u9762\u5fae\u73af\u5883\u4e2d\u7684\u514d\u75ab\u7a33\u6001\uff0c\u663e\u8457\u52a0\u901f\u4e86\u521b\u9762\u6108\u5408\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"841\" src=\"http:\/\/www.cailiaoniu.com\/wp-content\/uploads\/2025\/11\/bf2e94a56e16c6a-1.png\" alt=\"\" class=\"wp-image-269279\"\/><\/figure>\n\n\n\n<p><strong>\u56fe<\/strong><strong>8. CHA-Zn NPs<\/strong><strong>\u91cd\u7f16\u7a0b\u5de8\u566c\u7ec6\u80de\u52a0\u901f\u7cd6\u5c3f\u75c5\u4f24\u53e3\u7684\u4f53\u5185\u6108\u5408<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6d88\u9664\u6d3b\u6027\u6c27\u7c07\uff08ROS\uff09\u548c\u8bf1\u5bfc\u6297\u708e\u578bM2\u578b\u5de8\u566c\u7ec6\u80de\u6781\u5316\u5bf9\u7ec4\u7ec7\u4fee\u590d\u81f3\u5173\u91cd\u8981\u3002\u7136\u800c\uff0c\u5728\u4f53\u5185\u7cbe\u786e\u8c03\u63a7\u8fd9\u4e9b\u8fc7\u7a0b\u6781\u5177\u6311\u6218\u6027\u3002\u5c3d\u7ba1\u5c0f\u7684\u751f\u7269\u5206\u5b50\u548c\u5177\u6709ROS\u6e05\u9664\u548cM2\u6781\u5316\u7535\u4f4d\u7684&#8230;<\/p>\n","protected":false},"author":31345,"featured_media":0,"comment_status":"open","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-269270","post","type-post","status-publish","format-standard","hentry","category-keji"],"_links":{"self":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/269270"}],"collection":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/users\/31345"}],"replies":[{"embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=269270"}],"version-history":[{"count":1,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/269270\/revisions"}],"predecessor-version":[{"id":269280,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=\/wp\/v2\/posts\/269270\/revisions\/269280"}],"wp:attachment":[{"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=269270"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=269270"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.cailiaoniu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=269270"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}