{"id":60250,"date":"2017-01-08T14:00:26","date_gmt":"2017-01-08T06:00:26","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=60250"},"modified":"2017-03-18T22:09:57","modified_gmt":"2017-03-18T14:09:57","slug":"nano-lett-%ef%bc%9a%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%e8%bd%ac%e5%90%91%e9%93%81%e6%ad%bb%e4%ba%a1-%e9%87%91%e5%b1%9e%e6%9c%89%e6%9c%ba%e7%bd%91%e7%bb%9c%e7%94%a8%e4%ba%8e%e9%ab%98%e6%95%88%e7%9a%84","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=60250","title":{"rendered":"Nano Lett.\uff1a\u7ec6\u80de\u51cb\u4ea1\u8f6c\u5411\u94c1\u6b7b\u4ea1-\u91d1\u5c5e\u6709\u673a\u7f51\u7edc\u7528\u4e8e\u9ad8\u6548\u7684\u6297\u764c\u6cbb\u7597"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/b><\/strong><\/p>\n

\u8c03\u8282\u7ec6\u80de\u6b7b\u4ea1\u7684\u5148\u8fdb\u6750\u6599\u5bf9\u6297\u764c\u6cbb\u7597\u7684\u53d1\u5c55\u6709\u7740\u91cd\u5927\u7684\u610f\u4e49\u3002\u6740\u6b7b\u764c\u7ec6\u80de\uff0c\u4f20\u7edf\u7684\u7b56\u7565\u662f\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\uff0c\u4f46\u662f\u7531\u4e8e\u764c\u7ec6\u80de\u7684\u7a81\u53d8\uff0c\u7ec6\u80de\u51cb\u4ea1\u7684\u9014\u5f84\u901a\u5e38\u662f\u53d7\u963b\u7684\u3002\u56e0\u6b64\uff0c\u8bf1\u5bfc\u7ec6\u80de\u574f\u6b7b\u8fd9\u4e00\u7b56\u7565\uff0c\u53ef\u4ee5\u7ed5\u8fc7\u7ec6\u80de\u51cb\u4ea1\u7684\u963b\u529b\u800c\u66f4\u597d\u5730\u6d88\u9664\u764c\u7ec6\u80de\u3002\u8fd1\u5e74\u6765\uff0c\u975e\u5178\u578b\u6027\u7a0b\u5e8f\u6027\u6b7b\u4ea1\u9014\u5f84\u4e2d\u7684\u94c1\u6b7b\u4ea1\uff0c\u8bc1\u660e\u53ef\u4ee5\u6709\u6548\u7684\u6740\u6b7b\u764c\u7ec6\u80de\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/b><\/strong><\/p>\n

\u6700\u8fd1\uff0c\u6b66\u6c49\u5927\u5b66\u5f20\u5148\u6b63\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/b><\/strong>\u7b49\u4eba\u53d1\u73b0\u6c27\u5316\u5e94\u6fc0\u8c03\u8282p53\u7684\u80fd\u529b\uff0c\u800c\u4e14\u82ac\u987f\u53cd\u5e94\u53ef\u4ee5\u8bf1\u5bfc\u91d1\u5c5e\u6709\u673a\u7f51\u7edc\uff08MON\uff09\u7684\u5f62\u6210\uff0c\u6b64\u7f51\u7edc\u53ef\u4ee5\u6709\u6548\u5c01\u88c5p53\u8d28\u7c92\uff08MON-p53\uff09\u901a\u8fc7\u94c1\u6b7b\u4ea1\/\u51cb\u4ea1\u6df7\u5408\u9014\u5f84\u5f7b\u5e95\u6e05\u9664\u764c\u75c7\u7ec6\u80de\u3002\u4f5c\u8005\u786e\u8ba4\u4e86MON-p53\u5f15\u8d77\u94c1\u6b7b\u4ea1\u7684\u8be6\u7ec6\u673a\u5236\u2014\u7eb3\u7c73\u9897\u7c92\u53ef\u4ee5\u5927\u5927\u6291\u5236\u8c37\u80f1\u7518\u80bd\u5408\u6210\u9014\u5f84\uff0c\u5bfc\u81f4\u751f\u7269\u819c\u7684\u8102\u8d28\u8fc7\u6c27\u5316\u800c\u5f15\u53d1\u6c27\u5316\u5e94\u6fc0\u89e6\u53d1\u94c1\u6b7b\u4ea1\uff0c\u800c\u4e14\u53d1\u73b0MON-p53\u4ecb\u5bfc\u201c\u65c1\u89c2\u8005\u6548\u5e94\u201d\u8fdb\u4e00\u6b65\u654f\u5316\u764c\u75c7\u7ec6\u80de\u8fdb\u884c\u94c1\u6b7b\u4ea1\u3002\u957f\u671f\u7684\u6297\u764c\u5b9e\u9a8c\u8868\u660eMON-p53\u6cbb\u7597\u4e0d\u4ec5\u53ef\u4ee5\u6291\u5236\u80bf\u7624\u7684\u751f\u957f\u8fd8\u53ef\u4ee5\u5ef6\u957f\u80bf\u7624\u8f74\u627f\u8001\u9f20\u7684\u5bff\u547d\u3002\u8be5\u6210\u679c\u4ee5\u201cSwitching Apoptosis to Ferroptosis: Metal\u2212Organic Network for High-Efficiency Anticancer Therapy\u201d\u4e8e12\u670828\u65e5\u53d1\u8868\u5728Nano Lett.\u671f\u520a\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/b><\/strong><\/p>\n

\u793a\u610f\u56fe1<\/b><\/strong>\u00a0 MON-p53\u4ecb\u5bfc\u6297\u764c\u6cbb\u7597\u7684\u793a\u610f\u56fe<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(\u2160) MON-p53\u00a0\u5185\u541e\u4f5c\u7528\u3002<\/p>\n

(\u2161) MON\u5f15\u8d77\u7684\u82ac\u987f\u53cd\u5e94\u3002<\/p>\n

(\u2162) \u8f6c\u67d3\u548c\u8868\u8fbep53\u86cb\u767d\u3002<\/p>\n

(\u2163) p53\u86cb\u767d\u4ecb\u5bfc\u6291\u5236SLC7A11\u86cb\u767d\u8de8\u819c\u3002<\/p>\n

(\u2164) \u82ac\u987f\u53cd\u5e94\u8c03\u8282LPO\u79ef\u7d2f\uff0cSLC7A11\u6291\u5236\u8bf1\u5bfcGSH\u6e05\u9664\u5f15\u8d77\u94c1\u6b7b\u4ea1\uff1bp53\u86cb\u767d\u8c03\u63a7\u51cb\u4ea1\u9014\u5f84\u548c\u5f15\u8d77\u51cb\u4ea1\u3002<\/p>\n

\u56fe1 \u00a0MON-p53\u7684\u5236\u5907\u548c\u8868\u5f81\u56fe<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(a) MON-p53\u5236\u5907\u7684\u793a\u610f\u56fe\u3002<\/p>\n

(b) PEI\/p53 \u7684\u900f\u5c04\u7535\u955c\u6210\u50cf\u56fe\u3002<\/p>\n

(c) MON-p53\u7684\u900f\u5c04\u7535\u955c\u6210\u50cf\u56fe\u3002<\/p>\n

(d-f) PEI-p53\u4ecb\u5bfc\u51cb\u4ea1\u7684\u7ec6\u80de\u5f62\u6001\u56fe\uff08p53\u6d53\u5ea6\u4e3a2ug\/ml\uff09\u3002<\/p>\n

(g-i) MON-p53\u4ecb\u5bfc\u94c1\u6b7b\u4ea1\u7684\u7ec6\u80de\u5f62\u6001\u56fe\uff08p53\u6d53\u5ea6\u4e3a2ug\/ml\uff09\u3002<\/p>\n

\u56fe<\/b><\/strong>2 \u00a0MON-p53 \u8bf1\u5bfc\u94c1\u6b7b\u4ea1\u7684\u76f8\u5173\u673a\u5236\u9a8c\u8bc1<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(a) MON-p53\u8bf1\u53d1\u94c1\u6b7b\u4ea1\u7684\u63a8\u5bfc\u7684\u673a\u5236\u56fe\u3002<\/p>\n

(b) MON-p53\u00b1 Fer\u3001Apo\u3001Nec\u3001Aut\u3001DFO\u3001\u00a0Glu\u3001Cys\u3001GSH\u3001VC\u3001VE\uff1bPEI\/p53\u00b1 Fer\u3001Apo\u5904\u7406\u540e\u7684HT1080\u7ec6\u80de\u5b58\u6d3b\u56fe\u3002<\/p>\n

(c )\u6d82\u8986PEI\/p53\u548cEra\u7684\u4e0d\u540c\u91d1\u5c5e\u6709\u673a\u7f51\u7edc\u5904\u7406\u540e\u7684HT1080\u7ec6\u80de\u5b58\u6d3b\u56fe\u3002<\/p>\n

(d) HT1080\u7ec6\u80de\u7ecfferric chloride \u3001PEI\/p53\u3001Fe(II)-MON-p53\u3001Fe(III)-MON-p53\u5904\u7406\u540e\u7684LPO\u3001\u00a0ROS\u3001 NADP+\/NADPH \u7684\u7ec4\u5206\u56fe\u3002<\/p>\n

(e) HT1080\u3001SCC-7\u3001\u00a04T1\u3001\u00a0COS7\u7ec6\u80de\u7ecfPEI\/p53\u3001\u00a0MON-p53\u3001\u00a0Erastin\u3001\u00a0Erastin + Fe(III)\u5904\u7406\u540e\u7684\u7ec6\u80de\u5b58\u6d3b\u56fe\u3002<\/p>\n

(f) HT1080\u7ec6\u80de\u4e0ePBS\u3001PEI\/p53\u3001\u00a0MONP\u3001MON-p53\u5171\u5b75\u80b2\u540e\u7684\u7ea2\u8272\u67d3\u6599BODIPY-C11\u67d3\u8272\u7684LPO\u6210\u50cf\u56fe\u3002<\/p>\n

\u56fe3 <\/b><\/strong>\u00a0<\/b><\/strong>MON-p53\u4ecb\u5bfc\u7684\u201c\u65c1\u89c2\u8005\u6548\u5e94\u201d\u9a8c\u8bc1\u5b9e\u9a8c<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a) TA\u3001GA\u3001EG\u3001\u00a0EGCG\u3001EA based MON-p53\u5904\u7406\u540e\u7684HT1080\u7ec6\u80de\u7684DCFH-DA\u68c0\u6d4b\u6210\u50cf\u56fe\u3002<\/p>\n

(b) HT1080\u7ec6\u80de\u5728\u6c27\u5316\u5e94\u6fc0\u4e0b\u7ecfTA\u3001GA\u3001EG\u3001\u00a0EGCG\u3001EA based MON-p53\u5904\u7406\u540e\u7684DCFH-DA\u68c0\u6d4b\u6210\u50cf\u56fe\u3002<\/p>\n

(c) TA\u3001GA\u3001EG\u3001\u00a0EGCG\u3001EA based MON-p53\u5904\u7406\u540e\u7684HT1080\u7ec6\u80de\u5b58\u6d3b\u56fe\u3002<\/p>\n

(d) HT1080\u7ec6\u80de\u7ecfTA\u3001GA\u3001EG\u3001\u00a0EGCG\u3001EA based MON-p53\u5904\u7406\u540eLPO, ROS,\u00a0NADP+\/NADPH \u7ec4\u5206\u56fe\u3002<\/p>\n

(e) Boyden\u5c0f\u5ba4\u5206\u6790\u68c0\u6d4b\u793a\u610f\u56fe\u3002<\/p>\n

(f) \u4e0b\u5ba4MON-p53\u5904\u7406\u7684HT1080\u7ec6\u80de\uff0c\u4e0a\u5ba4\u5171\u5b75\u80b2\/\u672a\u5171\u5b75\u80b2NS\u3001PBS\u3001 PEI\/p53\u3001MON-p53\u9884\u5904\u7406\u7684HT1080\u7ec6\u80de \u00a0\u4e0d\u540c\u65f6\u95f4\u6bb5\u7684\u7ec6\u80de\u76f8\u5bf9\u5b58\u6d3b\u56fe\u3002<\/p>\n

(g) HT1080\u7ec6\u80de\u7ecfPBS\u3001PEI\/p53\u3001MON-p53\u3001MON-p53 + SOD\u3001MON-p53 +catalase\u5904\u7406\u540e\u7684LPO\u7ec4\u5206\u56fe\u3002<\/p>\n

(h)HT1080\u7ec6\u80de\u7ecf\u4e0d\u540c\u8bd5\u5242\u5904\u7406\u540e\u7684NADPH\u7ec4\u5206\u56fe\u3002<\/p>\n

\u56fe<\/b><\/strong>4<\/b><\/strong>\u00a0<\/b><\/strong>\u80bf\u7624\u8f74\u627f\u8001\u9f20\u7684\u6297\u764c\u5b9e\u9a8c<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(a) \u6ce8\u5c04Era\u3001MONP\u3001DNA\u540eHT1080\u80bf\u7624\u8f74\u627f\u8001\u9f20\u7684\u5b58\u6d3b\u7387\u66f2\u7ebf\u56fe\u3002<\/p>\n

(b) 25\u5929\uff0c\u8001\u9f20HT1080\u80bf\u7624\u4f53\u79ef\u5927\u5c0f\u66f2\u7ebf\u56fe\u3002<\/p>\n

(c)\u00a010\u5929\u300120\u5929\u300130\u5929\u65f6\u7684HT1080\u80bf\u7624\u8f74\u627f\u8001\u9f20\u7684\u6210\u50cf\u56fe\u3002<\/p>\n

(d) \u5916\u5468\u8840\u5faa\u73af\u80bf\u7624\u7ec6\u80de\u3001\u80ba\u8f6c\u79fb\u548c\u809d\u8f6c\u79fb\u90fd\u5177\u6709\u5f88\u9ad8\u8f6c\u79fb\u76844T1-GFP\u80bf\u7624\u8f74\u627f\u8001\u9f20\u7684\u8367\u5149\u6210\u50cf\u56fe\u3002<\/p>\n

\u3010\u5c55\u671b\u3011<\/b><\/strong><\/p>\n

MON-p53\u80fd\u4fc3\u8fdb\u7ec6\u80de\u5185\u6c27\u5316\u5e94\u6fc0\u80fd\u529b\uff0c\u51cf\u5c11\u8840\u6db2\u8f6c\u79fb\u3001\u80ba\u8f6c\u79fb\u548c\u809d\u8f6c\u79fb\u3002\u5f15\u8d77\u94c1\u6b7b\u4ea1\u7684\u7eb3\u7c73\u9897\u7c92\u53ef\u4ee5\u7ed5\u8fc7\u4f20\u7edf\u6297\u764c\u8bd5\u5242\u7684\u8010\u836f\u6027\uff0c\u4e3a\u63d0\u9ad8\u4f20\u7edf\u764c\u75c7\u6cbb\u7597\u6548\u679c\u63d0\u4f9b\u4e00\u79cd\u65b0\u7684\u89c6\u89d2\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Switching Apoptosis to Ferroptosis: Metal\u2212Organic Network for High-Efficiency Anticancer Therapy <\/a>(Nano Lett., 2016, DOI: 10.1021\/acs.nanolett.6b04060)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u7ec4\u9093\u5b8f\u534e\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

\u53c2\u4e0e\u751f\u7269\u6750\u6599\u8bdd\u9898\u8ba8\u8bba\u6216\u4e86\u89e3\u751f\u7269\u6750\u6599\u5c0f\u7ec4\u62db\u52df\u8be6\u60c5\uff0c\u8bf7\u52a0\u5165\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u4ea4\u6d41\u7fa4\uff08124806506\uff09\u3002\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6750\u6599\u4eba\u7f51\u5411\u5404\u5927\u56e2\u961f\u8bda\u5fc3\u7ea6\u7a3f\uff0c\u8bfe\u9898\u7ec4\u6700\u65b0\u6210\u679c\u3001\u65b9\u5411\u603b\u7ed3\u3001\u56e2\u961f\u8bbf\u8c08\u3001\u5b9e\u9a8c\u6280\u80fd\u7b49\u7686\u53ef\u6295\u7a3f\uff0c\u8bf7\u8054\u7cfb\uff1a\u90ae\u7bb1tougao@cailiaoren.com \u6216 QQ\uff1a97482208\u3002<\/p>\n

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