{"id":9367,"date":"2016-04-22T17:00:53","date_gmt":"2016-04-22T09:00:53","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=9367"},"modified":"2016-04-22T16:49:24","modified_gmt":"2016-04-22T08:49:24","slug":"%e7%a8%8b%e4%b9%89%e4%ba%91acs-nano%ef%bc%9a%e4%bb%a5%e6%a0%91%e5%bd%a2%e9%ab%98%e5%88%86%e5%ad%90%e4%bd%9c%e4%b8%ba%e6%a8%a1%e6%9d%bf%e5%90%88%e6%88%90%e5%8f%af%e6%9c%89%e6%95%88%e7%81%ad%e6%b4%bb","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=9367","title":{"rendered":"\u7a0b\u4e49\u4e91ACS Nano\uff1a\u4ee5\u6811\u5f62\u9ad8\u5206\u5b50\u4f5c\u4e3a\u6a21\u677f\u5408\u6210\u53ef\u6709\u6548\u706d\u6d3b\u80bf\u7624\u7684\u8d85\u5c0f\u578b\u3001\u591a\u529f\u80fd\u5316\u5149\u70ed\u5242"},"content":{"rendered":"

\"111\"<\/p>\n

\u3010\u6458\u8981\u3011<\/span><\/strong><\/p>\n

\u8d85\u5c0f\u578b\u591a\u529f\u80fd\u7eb3\u7c73\u7c92\u5b50\u7528\u4e8e\u5149\u70ed\u764c\u75c7\u6cbb\u7597\u975e\u5e38\u6709\u524d\u666f\uff0c\u4f46\u8fd9\u4e9b\u7eb3\u7c73\u9897\u7c92\u7684\u5408\u6210\u4ecd\u7136\u5b58\u5728\u95ee\u9898\u3002\u6700\u8fd1\uff0c\u534e\u4e1c\u5e08\u8303\u5927\u5b66\u7a0b\u4e49\u4e91\u6559\u6388\u7b49\u4eba\u4f7f\u7528\u6811\u5f62\u805a\u5408\u7269\u4f5c\u4e3a\u6a21\u677f\u5408\u6210\u8d85\u5c0f\u5149\u70ed\u5242\uff0c\u5e76\u4f7f\u7528\u591a\u529f\u80fd\u56e2\u6539\u6027\u5904\u7406\u3002<\/p>\n

\u6811\u72b6\u5927\u5206\u5b50\u5c01\u88c5\u7eb3\u7c73\u7c92\u5b50\uff08\u5305\u62ec\u786b\u5316\u94dc\u3001\u94c2\u548c\u4e00\u4e2a\u5177\u67095nm\u5c3a\u5bf8\u7684\u94af\u7eb3\u7c73\u9897\u7c92\uff09\u663e\u793a\u51fa\u4f18\u5f02\u7684\u5149\u70ed\u6027\u80fd\u3002\u5229\u7528TAT\u6216RGD\u591a\u80bd\u5bf9\u5176\u8fdb\u884c\u6539\u6027\u4fc3\u8fdb\u7ec6\u80de\u6444\u53d6\u80fd\u529b\u548c\u5b9e\u73b0\u80bf\u7624\u9776\u5411\u7ed9\u836f\u3002\u4f7f\u7528\u8367\u5149\u63a2\u9488\u4fee\u9970\u4ee5\u5b9e\u73b0\u5b9e\u65f6\u6210\u50cf\u548c\u8ddf\u8e2a\u7c92\u5b50\u5206\u5e03\u3002\u8be5\u7814\u7a76\u8868\u660eRGD\u4fee\u9970\u7684DENPs\u5728\u8fd1\u7ea2\u5916\u7ebf\u7167\u5c04\u4e0b\u80fd\u6709\u6548\u5730\u6291\u5236\u80bf\u7624\u751f\u957f\u3002<\/p>\n

\u3010\u89e3\u8bfb\u3011<\/strong><\/p>\n

\u5149\u70ed\u6cbb\u7597\uff08PTT\uff09\u662f\u4e00\u79cd\u5f88\u6709\u524d\u9014\u7684\u764c\u75c7\u6cbb\u7597\u6a21\u5f0f\u3002\u4e3a\u4e86\u8fdb\u884c\u9ad8\u6548\u7684PTT\uff0c\u5728\u8fc7\u53bb\u5341\u5e74\u4e2d\u5df2\u5f00\u53d1\u51fa\u591a\u79cd\u5149\u70ed\u5242\uff08PAs\uff09\uff0c\u5305\u62ec\u91d1\u5c5e\u7eb3\u7c73\u7c92\u5b50\u3001\u78b3\u57fa\u6750\u6599\u3001\u534a\u5bfc\u4f53\u7eb3\u7c73\u9897\u7c92\u548c\u6709\u7eb3\u7c73\u7ed3\u6784\u7684\u805a\u5408\u7269\u3002\u8fd9\u4e9bPAs\u5747\u8868\u73b0\u51fa\u4f18\u826f\u7684\u5149\u70ed\u6548\u5e94\u548c\u5f7b\u5e95\u7684\u706d\u6d3b\u80bf\u7624\u80fd\u529b\uff0c\u4f46\u7531\u4e8e\u9897\u7c92\u5c3a\u5bf8\u76f8\u5bf9\u8f83\u5927\u3001\u591a\u529f\u80fd\u5316\u96be\u4ee5\u5b9e\u73b0\u7b49\u95ee\u9898\uff0c\u5b83\u4eec\u5f88\u5c11\u88ab\u7528\u4e8e\u4e34\u5e8a\u3002<\/p>\n

\u9897\u7c92\u5927\u5c0f\u663e\u8457\u5f71\u54cd\u4e86PAs\u5728\u4f53\u5185\u7684\u836f\u7269\u5b66\u884c\u4e3a\u3002PAs\u5728\u4e00\u7ef4\u4e0a\u7684\u5c3a\u5bf8\u4e00\u822c\u5927\u4e8e40nm\uff0c\u96be\u4ee5\u6df1\u5165\u80bf\u7624\u7ec4\u7ec7\u4e14\u96be\u4ee5\u88ab\u6e05\u9664\u51fa\u4f53\u5916\uff0c\u4ece\u800c\u964d\u4f4e\u4e86\u6cbb\u7597\u6548\u679c\u5e76\u589e\u52a0\u4e86\u6f5c\u5728\u7684\u6bd2\u6027\u3002\u4f46\u8fd9\u4e2a\u95ee\u9898\u53ef\u4ee5\u901a\u8fc7\u51cf\u5c0f\u7c92\u5f84\u6765\u89e3\u51b3\u3002<\/p>\n

\u636e\u8bc1\u5b9e\uff0c\u5c0f\u4e8e10nm\u7684\u7eb3\u7c73\u9897\u7c92\u53ef\u4ee5\u6e17\u900f\u5230\u80bf\u7624\u7684\u66f4\u6df1\u533a\u57df\uff0c\u4e0e\u8f83\u5927\u7c92\u5f84\u5c3a\u5bf8\u7684\u9897\u7c92\u76f8\u6bd4\uff0c\u66f4\u5bb9\u6613\u6709\u6548\u5730\u88ab\u80bf\u7624\u7ec6\u80de\u5185\u5316\uff0c\u4e5f\u53ef\u4ee5\u5feb\u901f\u6e05\u7406\u51fa\u4f53\u5185\u3002\u540c\u65f6\uff0c\u8d85\u5c0f\u7684\u4f53\u79ef\u4e5f\u4f7f\u5b83\u4eec\u80fd\u591f\u9003\u8131\u7f51\u72b6\u5185\u76ae\u7cfb\u7edf\u7684\u6355\u83b7\uff08\u4f8b\u5982\uff0c\u809d\uff0c\u813e\uff09\uff0c\u4ee5\u63d0\u9ad8\u5b83\u4eec\u5728\u80bf\u7624\u7ec6\u80de\u4e2d\u7684\u79ef\u7d2f\u3002<\/p>\n

\u591a\u529f\u80fd\u5316\u8d4b\u4e88PAs\u5f88\u591a\u9644\u52a0\u529f\u80fd\uff0c\u5305\u62ec\u8bca\u65ad\u3001\u6210\u50cf\u3001\u9776\u5411\u7ed9\u836f\u3001\u8ddf\u8e2a\u7c92\u5b50\u7684\u836f\u7269\u5b66\u884c\u4e3a\u548c\u8bc4\u4f30\u6cbb\u7597\u6548\u679c\u3002\u9274\u4e8e\u4e0a\u8ff0\u4f18\u52bf\uff0c\u591a\u529f\u80fd\u7eb3\u7c73\u7c92\u5b50\u5728\u4e2a\u6027\u5316\u6cbb\u7597\u4e2d\u5f88\u6709\u53d1\u5c55\u524d\u666f\u3002<\/p>\n

\u7136\u800c\uff0c\u9ad8\u8d28\u91cf\u591a\u529f\u80fd\u5316\u4ecd\u662f\u4e00\u4e2a\u5de8\u5927\u7684\u6311\u6218\u3002\u591a\u529f\u80fd\u610f\u5473\u7740\u9644\u52a0\u6210\u672c\u589e\u5927\u548c\u591a\u5408\u6210\u6b65\u9aa4\u589e\u591a\uff0c\u7279\u522b\u662f\u5bf9\u4e8e\u8d85\u5c0f\u7eb3\u7c73\u9897\u7c92\uff0c\u5728\u5b83\u4eec\u7684\u8868\u9762\u5f88\u96be\u6392\u5217\u4e0d\u540c\u7684\u529f\u80fd\u56e2\u3002\u56e0\u6b64\uff0c\u5408\u6210\u7684\u591a\u529f\u80fd\u7eb3\u7c73\u7c92\u5b50\u53ef\u80fd\u5177\u6709\u4e0d\u7406\u60f3\u7684\u836f\u7269\u52a8\u529b\u5b66\u884c\u4e3a\u5e76\u5728\u4f53\u5185\u5f15\u8d77\u4e25\u91cd\u7684\u4e0d\u826f\u53cd\u5e94\u3002<\/p>\n

\u6811\u5f62\u805a\u5408\u7269\u5177\u6709\u826f\u597d\u7684\u5f62\u72b6\u5c3a\u5bf8\u3001\u4e2d\u7a7a\u7684\u5185\u90e8\u548c\u9ad8\u5bc6\u5ea6\u5206\u5e03\u7684\u8868\u9762\u529f\u80fd\u56e2\uff0c\u4f7f\u5176\u53ef\u4f5c\u4e3a\u6a21\u677f\u5408\u6210\u5177\u6709\u9ad8\u5355\u5206\u6563\u6027\u548c\u7a33\u5b9a\u6027\u7684\u8d85\u5c0f\u7eb3\u7c73\u9897\u7c92\uff08\u901a\u5e38<5nm\uff09\u3002\u672c\u7814\u7a76\u9996\u5148\u4f7f\u7528\u6811\u5f62\u805a\u5408\u7269\u5408\u6210\u8d85\u5c0f\u7684\u5149\u70ed\u5242\uff08UPAs\uff09\uff0c\u5305\u62ec\u786b\u5316\u94dc\uff08CuS\uff09\u3001\u94c2\uff08Pt\uff09\u548c\u94af\uff08Pd\uff09\u7eb3\u7c73\u9897\u7c92\uff0c\u7136\u540e\u7528\u591a\u529f\u80fd\u57fa\u56e2\u6539\u6027\u5b83\u4eec\u3002<\/p>\n

\u6811\u5f62\u5927\u5206\u5b50\u5c01\u88c5\u94c2\u7eb3\u7c73\u7c92\u5b50\uff08DEPt\uff09\u540e\u5177\u6709\u4f18\u5f02\u7684\u5149\u70ed\u6548\u5e94\u548c\u826f\u597d\u7684\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u6210\u4e3a\u6027\u80fd\u6700\u4f18\u7684UPA\u3002DEPt\u8fdb\u4e00\u6b65\u7528\u4e0d\u540c\u80bf\u7624\u9776\u5411\u80bd\u4fee\u9970\uff08\u5373\u53cd\u5f0f\u8f6c\u5f55\u6fc0\u6d3b\u56e0\u5b50\uff08TAT\uff09\u548c\u73af\u72b6\u7cbe\u6c28\u9178-\u7518\u6c28\u9178-\u5929\u51ac\u6c28\u9178\uff08RGD\uff09\u80bd\uff09\uff0c\u5206\u522b\u5f97\u5230TAT-DEPt\u548cRGD-DEPt\u3002<\/p>\n

\u4f7f\u7528\u8367\u5149\u63a2\u9488\u8ffd\u8e2a\u4e86\u7ec6\u80de\u5185TAT-DEPt\u548cRGD-DEPt\u7684\u4f20\u9012\uff0c\u5e76\u5728\u8fd1\u7ea2\u5916\u6ce2\u6bb5\u7167\u5c04\u4e0b\uff08NIR\uff09\u5bf9\u5b83\u4eec\u7684\u4f53\u5916\u7ec6\u80de\u6740\u4f24\u529f\u6548\u8fdb\u884c\u4e86\u6d4b\u5b9a\u3002\u6df1\u5165\u7814\u7a76\u5b83\u4eec\u7684\u80bf\u7624\u9776\u5411\u6548\u7387\u548c\u706d\u6d3b\u80bf\u7624\u7684\u80fd\u529b\u3002<\/p>\n

\u3010\u5236\u5907\u65b9\u6cd5\u3011<\/strong><\/p>\n

1. G5-NH2\u805a\u915e\u80fa-\u80fa\u578b\u6811\u5f62\u9ad8\u805a\u7269\u7684\u5408\u6210<\/strong><\/p>\n

\u5c06G5-NH2\u6811\u5f62\u9ad8\u805a\u7269\uff08200mg\uff0c0.007mmol\uff09\u6eb6\u89e3\u4e8e3ml\u65e0\u6c34\u7532\u9187\uff0c\u518d\u52a0\u5165\u4e09\u4e59\u80fa\uff08127\u03bcL\uff0c0.911mmol\uff09\u548c\u4e59\u9178\u9150\uff0869\u03bcL\uff0c0.728mmol\uff09\u3002\u5c06\u8be5\u6df7\u5408\u7269\u5728\u5ba4\u6e29\u4e0b\u6405\u62cc48\u5c0f\u65f6\uff0c\u7136\u540e\u8f6c\u79fb\u5230\u900f\u6790\u888b\u4e2d\uff0c\u7528\u53bb\u79bb\u5b50\u6c34\u900f\u679010\u6b21\u3002<\/p>\n

2. \u6811\u5f62\u5c01\u88c5\u91d1\u5c5e\u7eb3\u7c73\u7c92\u5b50\u7684\u5408\u6210<\/strong><\/p>\n

DECuS\u662f\u901a\u8fc7\u5171\u6c89\u6dc0\u6cd5\u5236\u5907\u7684\u3002\u9996\u5148\uff0c\u5c060.354ml\u7684CuCl2\uff08100mM\uff09\u52a0\u5165\u52301.700mL\u7684G5-NH2-AC100\uff080.163mmol\uff09\u6c34\u6eb6\u6db2\u4e2d\uff0c\u5728\u5ba4\u6e29\u78c1\u6027\u6405\u62cc\u6761\u4ef6\u4e0b\u9010\u6ef4\u52a0\u51650.884ml\u7684NaHS\uff0880mM\uff09\uff0c\u968f\u540e\u989c\u8272\u7acb\u5373\u4ece\u6d45\u84dd\u8272\u53d8\u4e3a\u6df1\u8910\u8272\u3002<\/p>\n

30min\u540e\uff0c\u5c06\u53cd\u5e94\u6eb6\u6db2\u8f6c\u79fb\u5230\u6cb9\u6d74\u4e2d\u5e76\u572890\u2103\u52a0\u70ed15\u5206\u949f\u3002\u6700\u540e\uff0c\u5f97\u5230\u6697\u7eff\u8272\u6eb6\u6db2\u3002\u5728\u5ba4\u6e29\u4e0b\u51b7\u5374\u540e\uff0c\u5c06\u53cd\u5e94\u6eb6\u6db2\u8f6c\u79fb\u5230\u900f\u6790\u888b\u4e2d\u7528\u53bb\u79bb\u5b50\u6c34\u900f\u6790\u3002\u6536\u96c6\u6700\u7ec8\u4ea7\u7269\u5e76\u57284\u2103\u4e0b\u4fdd\u5b58\u3002<\/p>\n

\u6807\u51c6\u7684DEPt\u5408\u6210\u6b65\u9aa4\u662f\uff1a\u4f7f\u7528\u78c1\u529b\u6405\u62cc\uff0c\u57280.150mLG5-NH2-AC100\u6c34\u6eb6\u6db2\uff080.573mM\uff09\u4e2d\u52a0\u51650.553Ml\u7684K2PtCl6\uff0820 mM\uff09\u3002 24\u5c0f\u65f6\u540e\uff0c\u5728\u53cd\u5e94\u6eb6\u6db2\u4e2d\u52a0\u51650.553mL\u7684NaBH4\uff08200 mM\uff09\u6eb6\u6db2\u30022h\u540e\uff0c\u5c06\u53cd\u5e94\u6eb6\u6db2\u8f6c\u79fb\u5230\u900f\u6790\u888b\uff0c\u5e76\u7528\u53bb\u79bb\u5b50\u6c34\u900f\u6790\u3002\u6536\u96c6\u6700\u7ec8\u4ea7\u7269\u5e76\u57284\u2103\u4e0b\u4fdd\u5b58\u3002<\/p>\n

3. TAT\u6216RGD\u4fee\u9970\u7684\u6811\u5f62\u805a\u5408\u7269\u7684\u5408\u6210<\/strong><\/p>\n

G5-NH2AC100\uff0837.00mg\uff0c1.117\u03bcmol\uff09\u5206\u6563\u57283.0mL\u542b\u67093mM EDTA\u00b72Na\u6eb6\u6db2\uff08pH 7.2\uff09\u7684PBS\u7f13\u51b2\u6db2\u4e2d\u3002\u5c06\u6eb6\u89e3\u4e8eDMSO\u6eb6\u6db2\u4e2d\u7684SMCC\uff082.24mg\uff0c7\u03bcmol\uff09\u52a0\u5165\u4e0a\u8ff0\u6eb6\u6db2\u4e2d\uff0c\u7136\u540e\u5c06\u53cd\u5e94\u6eb6\u6db2\u5728\u5ba4\u6e29\u4e0b\u6405\u62cc24h\u3002<\/p>\n

\u5c06TAT\uff0813.93mg\uff0c8\u03bcmol\uff09\u6216RGD\uff084.85mg\uff0c8\u03bcmol\uff09\u52a0\u5165\u5230\u53cd\u5e94\u6eb6\u6db2\u4e2d\uff0c\u5e76\u5c06\u6df7\u5408\u7269\u5728\u5ba4\u6e29\u4e0b\u6405\u62cc24h\u3002\u5c06\u8be5\u6eb6\u6db2\u88c5\u5165\u900f\u6790\u888b\u7528\u53bb\u79bb\u5b50\u6c34\u900f\u6790\u4ee5\u9664\u53bb\u6e38\u79bb\u7684\u80bd\u3002<\/p>\n

4. TAT\u6811\u5f62RBITC\u548cRGD\u6811\u5f62RBITC\u7684\u5236\u5907<\/strong><\/p>\n

\u901a\u8fc7\u5728TAT-\u6811\u5f62\u805a\u5408\u7269\u6eb6\u6db2\u4e2d\uff081.10\u03bcM\uff09\u52a0\u5165RBITC\uff085.50\u03bcM\uff09\u5236\u5907TAT-\u6811\u5f62\u5927\u5206\u5b50RBITC\u3002\u8be5\u6df7\u5408\u7269\u5728\u5ba4\u6e29\uff0c\u9ed1\u6697\u73af\u5883\u4e2d\u6405\u62cc24h\u3002\u672a\u53cd\u5e94\u7684RBITC\u901a\u8fc7\u591a\u6b21\u53bb\u79bb\u5b50\u6c34\u900f\u6790\u9664\u53bb\u3002RGD-\u6811\u5f62\u5927\u5206\u5b50-RBITC\u901a\u8fc7\u76f8\u540c\u65b9\u6cd5\u5236\u5907\u3002<\/p>\n

5. TAT-DEPt\u548cRGD-DEPt\u7684\u5236\u5907<\/strong><\/p>\n

TAT-DEPt \u548c RGD-DEPt\u7684\u5236\u5907\u4e0eDEPt\u7684\u5408\u6210\u65b9\u6cd5\u76f8\u4f3c\u3002\u5c060.553mLK2PtCl6\uff0820mM\uff09\u52a0\u5165\u52300.150mLTAT-\u6811\u72b6\u7269\u6216RGD-\u6811\u72b6\u7269\uff080.573mM\uff09\u4e2d\u3002<\/p>\n

24h\u540e\uff0c\u5c060.553mL\u7684NaBH4\uff08200 mM\uff09\u6eb6\u6db2\u52a0\u5165\u5230\u8be5\u53cd\u5e94\u6eb6\u6db2\u6405\u62cc2h\u3002\u7136\u540e\u5c06\u53cd\u5e94\u6eb6\u6db2\u8f6c\u79fb\u5230\u4e00\u4e2a\u900f\u6790\u888b\uff0c\u5e76\u7528\u53bb\u79bb\u5b50\u6c34\u900f\u6790\u3002\u6536\u96c6\u6700\u7ec8\u4ea7\u7269\u5e76\u57284\u2103\u4e0b\u4fdd\u5b58\u3002\u3002<\/p>\n

6. RGD-DEPt-Cy5.5\u7684\u5236\u5907<\/strong><\/p>\n

\u901a\u8fc7\u5728\u53bb\u79bb\u5b50\u6c34\u4e2d\u6df7\u54080.005mLCy5.5 N-\u7f9f\u57fa\u7425\u73c0\u9170\u4e9a\u80fa\u916f(0.7mM)\u548c0.15mL RGD-DEPT\uff080.7 mM\uff09\uff0c\u5728\u9ed1\u6697\u73af\u5883\u4e0b\u53cd\u5e9424\u5c0f\u65f6\u83b7\u5f97RGD-DEPT-Cy5.5\u3002<\/p>\n

\u3010\u7ed3\u679c\u7b80\u6790\u3011<\/strong><\/p>\n

1. \u8868\u5f81<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe1 \u00a0\u591a\u529f\u80fdUPAs\u7684\u5236\u5907\u793a\u610f\u56fe<\/p>\n

\u4e59\u9170\u5316\u7684\u6811\u5f62\u5927\u5206\u5b50\u5148\u5c01\u88c5\u7eb3\u7c73\u7c92\u5b50\uff08\u5305\u62ec\u786b\u5316\u94dc\u3001\u94c2\u548c\u94af\u7eb3\u7c73\u9897\u7c92\uff09\uff0c\u518d\u8868\u9762\u6539\u6027\u5904\u7406\uff0c\u6700\u7ec8\u5f97\u5230\u5177\u6709\u8d85\u5c0f\u5c3a\u5bf8\u3001\u591a\u529f\u80fd\u4e14\u9ad8\u7a33\u5b9a\u6027\u7684UPAs\u3002<\/p>\n

\"\"\u56fe2 \u00a0DECuS\u3001DEPt\u3001DEPd\u7684TEM\u56fe\u50cf<\/p>\n

\u8be5\u63d2\u56fe\u663e\u793a\u5355DENP\u7684\u9ad8\u5206\u8fa8\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\u56fe\u50cf\u3002\u7528\u5171\u6c89\u6dc0\u6cd5\u5236\u5907\u4e86\u6811\u72b6\u5927\u5206\u5b50\u5c01\u88c5\u7eb3\u7c73CuO\uff08DECuS\uff09\u3002<\/p>\n

\u8be5\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\uff08TEM\uff09\u56fe\u50cf\u793a\u51faDECuS\u67094.5\u00b13.0nm\u7684\u5e73\u5747\u8d85\u5c0f\u5c3a\u5bf8\uff0c0.33nm\u7684\u6676\u683c\u6761\u7eb9\uff0c\u8fd9\u4e0e\u516d\u89d2CuS\u7684\uff08102\uff09\u9762\u4e00\u81f4\u3002DEPt\u67091.4\u00b10.3nm\u7684\u8d85\u5c0f\u578b\u5c3a\u5bf8\uff0cDEPd\u7684\u5c3a\u5bf8\u4e3a1.5\u00b10.5nm\u3002<\/p>\n

\u9ad8\u5206\u8fa8\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\u56fe\u50cf\u663e\u793a\uff0cDEPt\u548cDEPd\u7684\u6676\u683c\u6761\u7eb9\u5206\u522b\u4e3a0.22\u548c0.21nm\u3002<\/p>\n

2. DENPs\u7684\u5149\u70ed\u6548\u7387<\/strong><\/p>\n

\"3\"<\/p>\n

\u56fe3 \u00a0\u60ac\u6d6e\u4e8e\u53bb\u79bb\u5b50\u6c34\uff08300\u00b5M\uff0c1mL\uff09\u4e2d\u7684DENPs\u7684\u6e29\u5ea6\u53d8\u5316<\/p>\n

\u53bb\u79bb\u5b50\u6c34\u548c\u6811\u5f62\u805a\u5408\u7269\u6eb6\u6db2\u7528\u4f5c\u5bf9\u7167\u3002\u901a\u8fc7\u66b4\u9732\u5728NIR\u5149\u4e0b\uff0cDECuS\u3001DEPt\u548cDEPd\u60ac\u6d6e\u7269\u7684\u6e29\u5ea6\u8fc5\u901f\u63d0\u9ad8\u5230\u4e00\u4e2a\u5f88\u9ad8\u503c\uff0c\u5206\u522b\u4e3a45.6\u300147.0\u548c43.8\u2103\uff0c\u800c\u53bb\u79bb\u5b50\u6c34\u548c\u6811\u5f62\u805a\u5408\u7269\u6eb6\u6db2\u663e\u793a\u589e\u5f3a\u6700\u5c0f\u3002\u7ed3\u679c\u8868\u660e\uff0cDENPs\u80fd\u9ad8\u6548\u5730\u5c06NIR\u5149\u8f6c\u5316\u4e3a\u70ed\u80fd\u3002<\/p>\n

3. \u7ec6\u80de\u6bd2\u6027<\/strong><\/p>\n

NIH3T3\u548cPC-9\u7ec6\u80de\u63a5\u79cd\u572896\u5b54\u677f\u4e2d\uff0c\u5b9e\u9a8c\u524d\u4e00\u5929\uff0c\u6bcf\u4e2a\u5b54\u7684\u5bc6\u5ea6\u4e3a10000\u4e2a\u7ec6\u80de\u3002\u7ec6\u80de\u7528PBS\u6d17\u6da4\u4e00\u6b21\uff0c\u7136\u540e\u572837\u2103\u4e0b\u4e0eDENPs\u4e00\u8d77\u57f9\u517b\u5728\u6d53\u5ea6\u8303\u56f4\u4e3a0-400\u00b5M\u7684\u5b54\u677f\u4e2d24\u5c0f\u65f6\u3002\uff08\u76f8\u5f53\u4e8e\u6811\u5f62\u805a\u5408\u7269\u7684\u6d53\u5ea6\u8303\u56f4\u662f0-3.906\u00b5M\uff09<\/p>\n

\"4\"\u56fe4 \u00a0\u4e09\u79cdDENPs\u5728NIH3T3\u7ec6\u80de\u4e2d\u7684\u7ec6\u80de\u6bd2\u6027<\/p>\n

\u901a\u8fc7\u5bf9NIH3T3\u7684\u6b63\u5e38\u7ec6\u80de\u7cfb\u548cPC-9\u7684\u764c\u7ec6\u80de\u7cfb\u4f7f\u7528\u6807\u51c6\u76843-\uff084,5-\u4e8c\u7532\u57fa\u5421\u5576-2-\u57fa\uff09-2,5-\u4e8c\u82ef\u57fa\u6eb4\u5316\u7269\uff08MTT\uff09\u6cd5\u5bf9DENPs\u7684\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u8fdb\u884c\u8bc4\u4f30\u3002\u7ed3\u679c\u8868\u660e\uff0cDEPt\u548cDEPd\u57280-400\u00b5m\u7684\u6d53\u5ea6\u8303\u56f4\u5185\u5bf9\u4e24\u79cd\u7ec6\u80de\u7cfb\u7684\u6bd2\u6027\u53ef\u5ffd\u7565\uff0c\u800cDECuS\u5728\u9ad8\u6d53\u5ea6\u65f6\u662f\u6709\u6bd2\u7684\u3002<\/p>\n

4. \u4f7f\u7528DEPt\u5149\u70ed\u6740\u4f24\u80bf\u7624\u7ec6\u80de<\/strong><\/p>\n

PC-9\u7ec6\u80de\u63a5\u79cd\u572896\u5b54\u677f\u4e2d\uff0c\u5b9e\u9a8c\u524d\u4e00\u5929\uff0c\u6bcf\u4e2a\u5b54\u7684\u5bc6\u5ea6\u4e3a10000\u4e2a\u7ec6\u80de\u3002\u5c06\u7ec6\u80de\u4e0eDEPt\u572837\u2103\uff0c200\u03bcM\u7684\u6d53\u5ea6\u4e0b\u57f9\u80b24\u5c0f\u65f6\uff08\u76f8\u5f53\u4e8e\u6811\u5f62\u805a\u5408\u7269\u7684\u6d53\u5ea61.953\u03bcM\uff0c\u5149\u5bc6\u5ea6\u4e3a808nm\uff08OD808\uff09=0.225\uff09\uff0c\u7136\u540e\u5728\u529f\u7387\u5bc6\u5ea6\u4e3a6.4 W\u00b7cm\u22122\u7684808nm\u7684\u8fd1\u7ea2\u5916\u6fc0\u5149\u7167\u5c0410\u5206\u949f\u3002<\/p>\n

\u6b64\u5916\uff0c\u6fc0\u5149\u529f\u7387\u5bc6\u5ea6\u548c\u7167\u5c04\u65f6\u95f4\u662f\u53d8\u5316\u7684\uff0c\u4ee5\u8bc4\u4f30\u764c\u75c7\u6740\u4f24\u6548\u7387\u3002 NIR\u6cbb\u7597\u540e\uff0c\u518d\u5c06\u7ec6\u80de\u572837\u2103\u57f9\u517b2\u5c0f\u65f6\uff0c\u7136\u540e\u7528\u6807\u51c6MTT\u6cd5\u8fdb\u884c\u5206\u6790\u3002 \u4e3a\u4e86\u4f7f\u7528AO \/ EB\u53cc\u91cd\u67d3\u8272\u6cd5\uff0c\u5c06\u7ec6\u80de\u5728\u8fd1\u7ea2\u5916\u8f90\u5c04\u540e\u7528\u51b7PBS\u6d17\u6da4\u4e24\u6b21\uff0c\u7136\u540e\u7528AO\uff085\u03bcg\/mL\uff09\u548cEB\uff085\u03bcg\/mL\uff09\u67d3\u8272\u5904\u74063\u5206\u949f\u3002\u5728\u6b64\u4e4b\u540e\uff0c\u5c06\u7ec6\u80de\u518d\u7528\u51b7PBS\u6d17\u6da4\u4e24\u6b21\u5e76\u7528\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u3002<\/p>\n

5. \u4f53\u5916\u7ec6\u80de\u6444\u53d6TAT\u6811\u5f62RBITC\u548cRGD\u6811\u5f62RBITC<\/strong><\/p>\n

\"5\"<\/p>\n

\u56fe5 TAT\u80bd\u589e\u5f3a\u7ec6\u80de\u6444\u53d6DEPt\u3002 TAT\u5a92\u4ecb\u7ec6\u80de\u6444\u53d6DEPt\u793a\u610f\u56fe<\/p>\n

\"6\"
\n\u56fe 6 RGD\u80bd\u589e\u5f3a\u90e8\u7684\u7ec6\u80de\u6444\u53d6DEPt RGD\u5a92\u4ecb\u7ec6\u80de\u6444\u53d6DEPt\u7684\u793a\u610f\u56fe<\/p>\n

TAT\u80bd\u53ef\u4ee5\u7a7f\u900f\u7ec6\u80de\u819c\uff0c\u4fc3\u8fdb\u7ec6\u80de\u5bf9\u4e0d\u540c\u836f\u7269\u7684\u6444\u53d6\uff0c\u5728G5-NH2\u805a\u915e\u80fa-\u80fa\u578b\u6811\u5f62\u9ad8\u805a\u7269\u4e0a\u4fee\u65392.5 TAT\u80bd\u7684\u5e73\u5747\u6570\u76ee\uff0c\u8367\u5149\u63a2\u9488\u548c\u7f57\u4e39\u660eB\u5f02\u786b\u6c30\u9178\u916f\uff08BITC\uff09\u88ab\u8fdb\u4e00\u6b65\u4fee\u9970\u5728TAT-\u6811\u5f62\u805a\u5408\u7269\u4e0a\uff0c\u7528\u4e8e\u539f\u4f4d\u76d1\u63a7\uff08TAT-\u6811\u72b6RBITC\uff09\u3002<\/p>\n

\"7\"<\/p>\n

\u56fe 7 \u00a0RBITC\u53d1\u51fa\u7ea2\u8272\u7684\u8367\u5149\u3002\u808c\u52a8\u86cb\u767d\u4e1d\u88ab\u9b3c\u7b14\u73af\u80bd-FITC\uff08\u7eff\u8272\uff09\u67d3\u8272\uff0c\u7ec6\u80de\u6838\u88abDAPI\uff08\u84dd\u8272\uff09\u67d3\u8272<\/p>\n

NIH3T3\u7ec6\u80de\u548cPC-9\u7ec6\u80de\u5206\u522b\u7528TAT-\u6811\u72b6RBITC\u548c\u6811\u5f62\u5927\u5206\u5b50RBITC\u5904\u7406\u3002\u7ecf\u8fc74\u5c0f\u65f6\u7684\u6e29\u80b2\uff0c\u4ec5TAT-\u6811\u72b6RBITC\u88ab\u88ab\u4e24\u79cd\u7ec6\u80de\u66f4\u6709\u6548\u5730\u5438\u6536\u3002<\/p>\n

6. \u901a\u8fc7ICP-MS\uff08\u7535\u611f\u8026\u5408\u7b49\u79bb\u5b50\u4f53\u8d28\u8c31\uff09\u5206\u6790\u4f53\u5916\u7ec6\u80de\u6444\u53d6TAT-DEPt\u548c RGD-DEPt<\/strong><\/p>\n

\"8\"<\/p>\n

\u56fe 8 \u00a0\u6d4b\u5b9aPC-9\u7ec6\u80de\u4e2dPt\u7684\u542b\u91cf<\/p>\n

\u7528\u6811\u5f62TAT\u5236\u5907TAT-DEPt\uff0c\u5b83\u6709\u7c7b\u4f3c\u4e8eDEPt\u7684\u5927\u5c0f\u548c\u5149\u70ed\u6548\u5e94\u3002PC-9\u7ec6\u80de\u7528TAT-DEPt\u548cDEPt\u5904\u7406\uff0c\u7ec6\u80de\u5185\u7684Pt\u542b\u91cf\u901a\u8fc7\u7535\u611f\u8026\u5408\u7b49\u79bb\u5b50\u4f53\u8d28\u8c31\uff08ICP-MS\uff09\u6d4b\u5b9a\u3002\u7ed3\u679c\u663e\u793a\uff0c\u88abTAT-DEPt\u5904\u7406\u7684\u7ec6\u80de\u4e2d\u94c2\u542b\u91cf\u6bd4\u88abDEPt\u5904\u7406\u7684\u7ec6\u80de\u7684\u9ad8\u5f97\u591a\u3002<\/p>\n

7. \u4f7f\u7528TAT-DEPt\u6216RGD-DEPt\u5149\u70ed\u6740\u6b7b\u80bf\u7624\u7ec6\u80de<\/strong><\/p>\n

\"9\"<\/p>\n

\u56fe 9 PC-9\u7ec6\u80de\u5728NIR\u7167\u5c04\u540eAO \/ EB\u53cc\u91cd\u67d3\u8272\u7ed3\u679c<\/p>\n

\u5149\u70ed\u6740\u4f24\u5b9e\u9a8c\u8868\u660e\uff0c\u5728\u8fd1\u7ea2\u5916\u7167\u5c04\u524d\u53bb\u9664\u81ea\u7531\u7684TAT-DEPt\u3002\u7528TAT-DEPt\u5904\u7406\u7684PC-9\u7ec6\u80de\u5728\u7167\u5c04\u533a\u57df\u5168\u90e8\u88ab\u6740\u6b7b\uff08\u5982\u4e0a\u56fe\u53f3\uff0c\u6709\u767d\u8272\u865a\u7ebf\u5706\u6807\u5fd7\uff09\uff0c\u800c\u7528DEPt\u5904\u7406\u7684\u7ec6\u80de\u8868\u73b0\u51fa\u53ef\u4ee5\u5ffd\u7565\u4e0d\u8ba1\u7684\u7ec6\u80de\u6b7b\u4ea1\uff08\u5982\u4e0a\u56fe\uff0c\u5de6\uff09\u3002<\/p>\n

\u8fd9\u4e9b\u7814\u7a76\u8bc1\u5b9e\u4e86TAT\u80bd\u53ef\u4ee5\u63d0\u9ad8\u7ec6\u80de\u6444\u53d6DEPt\u7684\u80fd\u529b\uff0c\u5bfc\u81f4\u4f53\u5916\u66f4\u6709\u6548\u7684\u5149\u70ed\u6740\u6b7b\u764c\u7ec6\u80de\u3002<\/p>\n

8. \u4f53\u5185\u5149\u70ed\u706d\u6d3b\u80bf\u7624<\/strong><\/p>\n

\"10\"<\/p>\n

\"10-2\"<\/p>\n

\u56fe 10 \u00a0 \u00a0PTT\u6cbb\u7597\u540e\u5207\u9664\u80bf\u7624\uff0c\u80bf\u7624\u7684\u5f62\u8c8c\u548c\u91cd\u91cf<\/p>\n

\u80bf\u7624\u7ec4\u7ec7\u4e2dRGD-DEPT\u7ec4\uff08RGD+\uff09\u94c2\u7684\u542b\u91cf\u5747\u663e\u8457\u589e\u52a0\uff0c\u4f46\u5728\u809d\u548c\u813e12h\u548c24h\u4e24\u4e2a\u65f6\u95f4\u70b9\u5904RGD-DEPT\u7ec4\uff08RGD+\uff09\u94c2\u7684\u542b\u91cf\u4e0eDEPT\u7ec4\uff08RGD-\uff09\u76f8\u6bd4\u964d\u4f4e\u4e86\u3002\u4e0a\u8ff0\u5206\u6790\u8bc1\u660eRGD\u53ef\u4ee5\u6709\u6548\u5730\u63d0\u9ad8\u9776\u5411\u9012\u9001DEPt\u5230\u80bf\u7624\u7684\u80fd\u529b\u3002<\/p>\n

\"11\"<\/p>\n

\u56fe 11 \u00a0\u901a\u8fc7\u539f\u4f4d\u672b\u7aef\u6807\u8bb0\u6d4b\u5b9a\u6cbb\u7597\u540e\u51cb\u4ea1\u7684\u80bf\u7624\u7ec6\u80de\uff08\u7ea2\u8272\uff09<\/p>\n

\u4e0a\u56fe\u8868\u660e\uff0cRGD-DEPT\u53ef\u6709\u6548\u79ef\u7d2f\u5728\u80bf\u7624\u5e76\u5b9e\u73b0\u9ad8\u6548\u7684PTT\u3002<\/p>\n

\u3010\u603b\u7ed3\u3011<\/strong><\/p>\n

DENPs\u884d\u751f\u7684UPAs\u5305\u62ecDECuS\u3001DEPt\u548cDEPd\uff0c\u5177\u6709\u5c3a\u5bf8\u8d85\u5c0f\uff0c\u5355\u5206\u6563\u6027\u597d\u3001\u7a33\u5b9a\u6027\u548c\u5149\u70ed\u8f6c\u6362\u6548\u7387\u9ad8\u7b49\u4f18\u70b9\u3002\u5f97\u76ca\u4e8e\u6811\u5f62\u805a\u5408\u7269\u8868\u9762\u4e0a\u7684\u9ad8\u5bc6\u5ea6\u7684\u529f\u80fd\u56e2\uff0cDENPs\u53ef\u4ee5\u88ab\u4e0d\u540c\u7684\u529f\u80fd\u56e2\u4fee\u9970\uff0c\u5982\u7ec6\u80de\u7a7f\u819c\u80bd\u3001\u80bf\u7624\u9776\u5411\u80bd\u548c\u8367\u5149\u63a2\u9488\uff0c\u5b83\u4eec\u7684\u6570\u91cf\u548c\u4f4d\u7f6e\u53ef\u63a7\u5236\u3002<\/p>\n

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