{"id":96877,"date":"2017-08-19T06:30:38","date_gmt":"2017-08-18T22:30:38","guid":{"rendered":"http:\/\/www.cailiaoniu.com\/?p=96877"},"modified":"2017-08-17T15:17:50","modified_gmt":"2017-08-17T07:17:50","slug":"nano-lett-%ef%bc%9a%e5%9f%ba%e4%ba%8edna%e6%b0%b4%e5%87%9d%e8%83%b6%e7%9a%84%e5%be%aa%e7%8e%af%e8%82%bf%e7%98%a4%e7%bb%86%e8%83%9e%e8%af%86%e5%88%ab%e3%80%81%e6%8e%a9%e8%94%bd%e5%92%8c%e5%89%a5","status":"publish","type":"post","link":"http:\/\/www.cailiaoniu.com\/?p=96877","title":{"rendered":"Nano Lett.\uff1a\u57fa\u4e8eDNA\u6c34\u51dd\u80f6\u7684\u5faa\u73af\u80bf\u7624\u7ec6\u80de\u8bc6\u522b\u3001\u63a9\u853d\u548c\u5265\u79bb\u7528\u4e8e\u6d3b\u4f53\u7ec6\u80de\u5206\u6790"},"content":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011<\/strong><\/p>\n

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\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u6765\u81ea\u4e0a\u6d77\u4ea4\u901a\u5927\u5b66<\/strong>\u7684\u5de6\u5c0f\u78ca\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u7b49\u4eba\u5f00\u53d1\u4e86\u4e00\u79cd\u9002\u4f53\u89e6\u53d1\u94b3\u5939\u5f0f\u6742\u4ea4\u94fe\u53cd\u5e94\uff08atcHCR\uff09\u65b9\u6cd5\uff0c\u901a\u8fc7\u591a\u5b54DNA\u6c34\u51dd\u80f6\u7528\u4e8e\u8fdb\u884c\u539f\u4f4d\u9274\u5b9a\u548c\u968f\u540e\u7684\u63a9\u853d\/\u5265\u79bb\u3002\u7136\u540e\u5c06\u8fd9\u4e9b\u5904\u7406\u8fc7\u7684CTCs\u7528\u4e8e\u6d3b\u7ec6\u80de\u5206\u6790\u3002\u5728\u6b64\u8bbe\u8ba1\u4e2d\uff0c\u9002\u4f53\u672b\u7aef\u7684DNA\u77ed\u94fe\u7279\u5f02\u6027\u8bc6\u522bCTCs\u8868\u9762\u4e0a\u7684\u4e0a\u76ae\u7ec6\u80de\u7c98\u9644\u5206\u5b50\uff08EpCAM\uff09\uff0c\u5176\u901a\u8fc7\u8d77\u59cb\u7684\u5206\u652f\u8fc1\u79fb\u89e6\u53d1\u968f\u540e\u7684atcHCR\u3002\u591a\u5b54DNA\u6c34\u51dd\u80f6\u57fa\u4e8e\u5355\/\u7c07\u7684CTCs\u5141\u8bb8\u4ee5\u6700\u5c0f\u7684\u7ec6\u80de\u635f\u4f24\u76f4\u63a5\u6355\u83b7\u6d3b\u7684CTCs\u3002\u76f8\u5173\u6210\u679c\u4ee5\u9898\u4e3a\u201cDNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis<\/strong>\u201d\u53d1\u8868\u5728\u4e86Nano Letters<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 \u57fa\u4e8eDNA\u51dd\u80f6\u5316\u7684CTC\u63a9\u853d\u548c\u5265\u79bb<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u5728\u521d\u59cb\u6eb6\u6db2\u4e2d\uff0c\u5c06MCF-7\u7ec6\u80de\u5206\u6563\u5728\u6eb6\u6db2\u4e2d<\/p>\n

\uff08b\uff09DNA\u53d1\u5939H1\u548cH2\u88ab\u6355\u83b7\u5728\u4e9a\u7a33\u6001<\/p>\n

\uff08c\uff09\u5728\u7ec6\u80de\u819c\uff08\u7eff\u8272\uff09\u4e0a\u7ecfDiO\u67d3\u8272\u7684\u8102\u8d28\u4e0e\u9002\u4f53\u5f15\u53d1\u53cc\u5d4c\u6bb5\uff08\u7ea2\u8272\uff09\u7684\u5171\u805a\u7126\u56fe\u50cf<\/p>\n

\uff08d\uff09MCF-7\u7ec6\u80de3D\u5806\u53e0\u5728DNA\u6c34\u51dd\u80f6\u4e2d\uff0cFDA\u67d3\u8272\u4e3a\u7eff\u8272\uff0c\u5728\u6c34\u51dd\u80f6\u4e2d\u663e\u793a\u591a\u5c42\u7ec6\u80de<\/p>\n

\uff08e\uff09\u901a\u8fc7\u52a0\u5165ATP\uff0c\u5c06MCF-7\u7ec6\u80de\u91ca\u653e\u5e76\u5206\u6563\u5728\u6eb6\u6db2\u4e2d<\/p>\n

\u56fe2 CTC\u63a9\u853d\u901a\u8fc7\u5149\u5b66\u663e\u5fae\u955c\u548cSEM\u6210\u50cf<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09CTC\u63a9\u853d\u793a\u610f\u56fe\u548c3D\u5171\u805a\u7126\u663e\u5fae\u955c\u7684\u8868\u5f81<\/p>\n

\uff08b-e\uff09\u5728\u7ec6\u80de\u819c\uff08\u7eff\u8272\uff09\u4e0a\u7ecfDiO\u67d3\u8272\u7684\u8102\u8d28\u4e0e\u9002\u4f53\u5f15\u53d1\u53cc\u5d4c\u6bb5\uff08\u7ea2\u8272\uff09\u7684\u5171\u805a\u7126\u56fe\u50cf<\/p>\n

\uff08f\uff09\u7528DNA\u6c34\u51dd\u80f6\u63a9\u853d\u7684MCF-7\u7ec6\u80de\u7684ESEM\u8868\u5f81<\/p>\n

\uff08g\uff09SEM\u56fe\u50cf\u663e\u793a\u51b7\u51bb\u5e72\u71e5\u7684DNA\u6c34\u51dd\u80f6\u7684\u591a\u5b54\u7ed3\u6784<\/p>\n

\u56fe3 CTC\u91cf\u5316\u4e0e\u57fa\u4e8eDNA\u51dd\u80f6\u5316\u7684CTC\u63a9\u853d<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u4f7f\u7528AuNPs\u4f5c\u4e3a\u6c34\u51dd\u80f6\u7ec4\u4ef6\u7684\u53ef\u89c6\u5316\u6307\u6807<\/p>\n

\uff08b\uff09DNA\u6c34\u51dd\u80f6\u7684AuNPs\u5305\u5c01\u80fd\u529b\u4e0e\u8bf1\u53d1\u6c34\u51dd\u80f6\u7ec4\u88c5\u7684\u7ec6\u80de\u6570\u6709\u5173<\/p>\n

\uff08c\uff0cd\uff09\u8be5\u65b9\u6cd5\u53ef\u7528\u4e8e\u68c0\u6d4b\u8840\u6db2\u6837\u54c1\u4e2d\u7684MCF-7\u7ec6\u80de<\/p>\n

\u3010\u603b\u7ed3\u3011<\/strong><\/p>\n

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\u6587\u732e\u94fe\u63a5<\/strong>\uff1aDNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis<\/a>\uff08Nano Lett.,2017,DOI:10.1021\/acs.nanolett.7b01006\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u7ec4Allen\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

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